Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,409,156 G→A 6.7% noncoding (28/122 nt) fnrS → FNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,409,1560GA 6.7% 176.6 / 2.7 67noncoding (28/122 nt)fnrSFNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base A (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGA  >  NC_000913/1409010‑1409300
                                                                                                                                                  |                                                                                                                                                
ttGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTATCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCg                                                                                                                                                >  1:247193/1‑149 (MQ=255)
        tagtaAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTc                                                                                                                                        >  2:976002/1‑149 (MQ=255)
          gtaAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCTTTGCGCTcaa                                                                                                                                      >  2:664382/1‑147 (MQ=255)
            aaaTGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGGAAAGTTTGAGCGAAGTCAATAAAGTCTCTACCCATTCAGGGCAATATGTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGAGGAGCGCCata                                                                                                                                    >  2:526448/1‑149 (MQ=255)
             aaTGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAACCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCatat                                                                                                                                   >  1:592002/1‑149 (MQ=255)
                 gTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTc                                                                                                                               <  2:259818/149‑1 (MQ=255)
                        cAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTAGAGCGATGTCAATTAACTCTCTACCCAGTGACCGCCATAGCTCTCTAGCTGGTGAATGGAACGTCAAGCGATGGCCGTGGCGCTCCAGATTGTcttccttc                                                                                                                        >  2:921364/1‑149 (MQ=255)
                          aaaGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGGGTGAGCGAAGTCAATAAACTCTCTACCCAATCAGGGCAATATCTCTCTTGAAGGTGAATGCAACGTCAAGCGATGGGCGTTGAGCTCCATAACGTATTACTTcat                                                                                                                      >  1:680958/1‑147 (MQ=255)
                           aaGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtt                                                                                                                     >  2:554644/1‑149 (MQ=255)
                              cAATTTTTCCGGCCGTCTGTATACAAACACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCttttt                                                                                                                  <  2:372191/149‑1 (MQ=255)
                               aaTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGATTGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtttttt                                                                                                                 <  1:664382/149‑1 (MQ=255)
                               aaTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtttttt                                                                                                                 <  2:981970/149‑1 (MQ=255)
                               aaTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtttttt                                                                                                                 <  2:500122/149‑1 (MQ=255)
                                aTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCACTATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGGCTTACTTCCTTTTTTg                                                                                                                <  2:700954/149‑1 (MQ=255)
                                aTTTTTCCGGCTGTCTGAATACAAAAACGCAGCAAAGTTTGAGCGAATACAATAAACTCTCTACCCATTCAGGGAAATATATCTCTTGCAGCTGAATGCAACGTCCAGCAATGGTCGTTGCTCTACATATTTTATTACTTACttatttt                                                                                                                >  2:415322/1‑148 (MQ=255)
                                  ttttCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGaa                                                                                                              <  1:387419/149‑1 (MQ=255)
                                    ttCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAAtt                                                                                                            <  2:143156/149‑1 (MQ=255)
                                       cGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACt                                                                                                         >  1:586997/1‑149 (MQ=255)
                                       cGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACt                                                                                                         <  2:388738/149‑1 (MQ=255)
                                            gtcttgataCAAAAACGCCGCACAGTTTGAGGGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCTTa                                                                                                    <  2:844766/143‑1 (MQ=255)
                                                gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                           >  1:173661/1‑105 (MQ=255)
                                                gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                           <  2:173664/106‑2 (MQ=255)
                                                gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                           <  2:173661/106‑2 (MQ=255)
                                                gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTAAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                           >  1:173664/1‑105 (MQ=255)
                                                  ataCAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACaa                                                                                              >  1:597714/1‑149 (MQ=255)
                                                  ataCAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGACAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTACTTTTTTGAATTACTGCATAGCACaa                                                                                              >  1:993226/1‑149 (MQ=255)
                                                    aCAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAAtt                                                                                            <  1:1047293/149‑1 (MQ=255)
                                                      aaaaaCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGa                                                                                          >  1:412277/1‑149 (MQ=255)
                                                       aaaaCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGAt                                                                                         <  1:254832/149‑1 (MQ=255)
                                                         aaCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTc                                                                                       >  2:1121967/1‑149 (MQ=255)
                                                            gccgcAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTa                                                                                    <  1:976002/149‑1 (MQ=255)
                                                             ccgaAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTTCATGTGAATGCAACGTCAATCGATTGGCGTTGCGATCCATATTGTCTTACTTCCTTTTTTGAATTACTTCATCGCACAATTGATTCGTac                                                                                   <  2:135053/149‑1 (MQ=255)
                                                               gcAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTacga                                                                                 <  2:730526/149‑1 (MQ=255)
                                                                cAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTacgac                                                                                >  1:995708/1‑149 (MQ=255)
                                                                     tttGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCgc                                                                           >  1:816482/1‑148 (MQ=255)
                                                                           cGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTtga                                                                     <  2:968258/149‑1 (MQ=255)
                                                                            gAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCCTCGCACAATTGATTCGTACGACGCCGACTTtgat                                                                    <  2:336505/149‑1 (MQ=255)
                                                                               gTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAg                                                                 >  1:776794/1‑149 (MQ=255)
                                                                                tCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGt                                                                >  1:611767/1‑149 (MQ=255)
                                                                                  aaTAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCg                                                              >  1:297431/1‑149 (MQ=255)
                                                                                      aaCTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATTTTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGAGGAGTCGGCtt                                                          >  2:185588/1‑149 (MQ=255)
                                                                                          ctgtACCCATTCAGGGAAATATCTCTCTTGGAGGTTAATGCAACGTGAAGCGATGGGCGTTGCGCTTGATATTGTCTTACTTCCTTTTTTGAATTTCTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttt                                                      <  2:58940/146‑1 (MQ=255)
                                                                                          ctctACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttt                                                      <  1:368916/149‑1 (MQ=255)
                                                                                           tctACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCttttttt                                                     <  1:554644/149‑1 (MQ=255)
                                                                                            ctACCCATTCATGGCAATATCTCTCTTGCCGGTGACAGCAACGTCAAGCGATGGGCGTTGCGCTGCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttttt                                                    <  1:689623/149‑1 (MQ=255)
                                                                                              aCCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGc                                                  >  2:237654/1‑149 (MQ=255)
                                                                                              aCCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGGCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGc                                                  <  2:237524/149‑1 (MQ=255)
                                                                                                ccATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCt                                                >  1:64784/1‑149 (MQ=255)
                                                                                                     cAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGTTGGTCGTTGCGCTCCATATTGTATTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGTCGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGttat                                           <  2:72698/149‑1 (MQ=255)
                                                                                                      aGGTCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTGCCTGttattt                                         <  1:526448/149‑1 (MQ=255)
                                                                                                         gCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACTCCGACTTTGATGACTCGGCTGTTTTTTGCCAGttatttat                                       >  2:896070/1‑149 (MQ=255)
                                                                                                               tctctcTTGCAGGTGAATGCAACGTCTAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTGCTTGTTTGAATTACTGGATAGCCCAATTGATTCGTACGACGCCGAGTGTGATGAGTCGGCTTTTTTTGGCCTGTTATTTATGAGCgg                                 >  2:138748/1‑148 (MQ=255)
                                                                                                                    ctTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTAcc                            >  1:1119677/1‑149 (MQ=255)
                                                                                                                     ttGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTAccc                           >  2:536331/1‑149 (MQ=255)
                                                                                                                      tGCAGGTGAATGCTACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTACTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCt                          <  1:273807/149‑1 (MQ=255)
                                                                                                                              aaTGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTTTCAGCGTCTACCCTTTAAGAGt                  >  2:340619/1‑149 (MQ=255)
                                                                                                                                tGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTcc                >  1:257239/1‑149 (MQ=255)
                                                                                                                                 gCAACGTCAAGCGATTGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCa               <  2:310477/149‑1 (MQ=255)
                                                                                                                                 gCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCCGCGTCTACCCTTTAAGAGTCCa               >  2:963035/1‑149 (MQ=255)
                                                                                                                                   aaCGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCCCAATTGATTCGTACTACGCCGACTTTGATGAGTCGGCGTTTTTTTGCCTGTTATTTATCAGCGTCTGCCCTTTAAGACTCcccc             >  2:797345/1‑149 (MQ=255)
                                                                                                                                       tCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAAt         >  1:372609/1‑149 (MQ=255)
                                                                                                                                       tCAAGAGATTGTCGTTGCGCTCCATATCGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTACGAGTCCACCCAAt         <  2:680958/149‑1 (MQ=255)
                                                                                                                                         aaGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCATCGTCTACCCTTTAATAGTCCACCCAATGa       >  1:527156/1‑149 (MQ=255)
                                                                                                                                          aGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGAc      <  2:257239/149‑1 (MQ=255)
                                                                                                                                          aGCGATGCGCGTTGCGCGCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGAc      <  2:397745/149‑1 (MQ=255)
                                                                                                                                           gctatcggCGGTGCGCACCATATTGTCTTACATCCTTTTTTGTATTACTGCAAAGCACAATTGATTCGTACGACGCAGACTTTGATGAGGCGGCTTTTTTTTGACTGTTAATTATCAGCGTCTACCCTTTAATAGTCCACCCAATGAcc     <  1:921364/143‑1 (MQ=255)
                                                                                                                                             gATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCag   >  1:1178755/1‑149 (MQ=255)
                                                                                                                                              aTGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCaga  <  2:297431/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGA  >  NC_000913/1409010‑1409300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: