Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,837 G→C 5.5% intergenic (‑94/+17) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8370GC 5.5% 216.0 / 3.9 73intergenic (‑94/+17)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (36/33);  new base C (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

TACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGC  >  NC_000913/1441690‑1441984
                                                                                                                                                   |                                                                                                                                                   
tacACATCCCGCCATCAGCATGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGTGATTATCTGAATCAGCTCCCCTGGAATGc                                                                                                                                                    <  1:443433/149‑1 (MQ=255)
tacACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGTCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGc                                                                                                                                                    <  1:1033543/149‑1 (MQ=255)
  cacaTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                                                                  <  1:962506/149‑1 (MQ=255)
     aTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTGATTGGGGATTATCTGAATCCGCTCCCCTGGAATGCAgggg                                                                                                                                               >  1:69107/1‑149 (MQ=255)
        ccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                                                                            <  1:689269/149‑1 (MQ=255)
           ccATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctga                                                                                                                                         <  2:454452/149‑4 (MQ=255)
            cATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCa                                                                                                                                        >  2:954349/1‑149 (MQ=255)
               cagcagGCTTAGCTCAAAAAACGCGGGTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATATAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                                                                     <  2:380843/149‑1 (MQ=255)
                 gcagGCTTAGCGCAACAACCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                                   <  1:943275/149‑1 (MQ=255)
                 gcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                                   >  2:273765/1‑149 (MQ=255)
                    ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                                                                                <  1:495183/149‑1 (MQ=255)
                    ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                                                                                <  1:435503/149‑1 (MQ=255)
                    ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                                                                                <  2:106781/149‑1 (MQ=255)
                     gCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                                                                               <  2:873867/149‑1 (MQ=255)
                            gcAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                                                                        >  2:627475/1‑149 (MQ=255)
                              aacaaACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgtt                                                                                                                      >  2:636944/1‑149 (MQ=255)
                              aacaaACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgtt                                                                                                                      <  1:1167693/149‑1 (MQ=255)
                                      cgGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAgg                                                                                                              <  2:723888/149‑1 (MQ=255)
                                            cTTTCTTCATTGGGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGAGGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCGGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGc                                                                                                        <  2:1063273/149‑1 (MQ=255)
                                             tttCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGAGGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                                                                       <  1:262043/149‑1 (MQ=255)
                                              ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCt                                                                                                      >  1:887792/1‑149 (MQ=255)
                                                   cATTGTGTTGATCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTTGTGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTc                                                                                                 <  2:880727/149‑1 (MQ=255)
                                                   cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTc                                                                                                 >  1:1031549/1‑149 (MQ=255)
                                                    aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                                                                                >  1:1154877/1‑149 (MQ=255)
                                                     ttGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCa                                                                                               >  1:1150854/1‑149 (MQ=255)
                                                      tgtgGTTGTCAATTACAGTTTCTGACTCATGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAg                                                                                              <  1:250971/149‑1 (MQ=255)
                                                      tgtgGTGCGCAATTACAGTTTCTGACTCAGGACTATTTTCAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAg                                                                                              <  1:555546/149‑1 (MQ=255)
                                                            tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCt                                                                                        >  1:314230/1‑149 (MQ=255)
                                                               cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                                                                     <  1:352746/149‑1 (MQ=255)
                                                                    aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                                                                                >  1:598676/1‑149 (MQ=255)
                                                                     cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                                                                               >  1:973855/1‑149 (MQ=255)
                                                                     cAGTGTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                                                                               <  1:610963/149‑1 (MQ=255)
                                                                        tttCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAg                                                                            <  2:567881/149‑1 (MQ=255)
                                                                            tGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTa                                                                        <  1:350628/149‑1 (MQ=255)
                                                                             gACTGAGGACTATTTTAAGAATTGAGGATGATTGGTCATTGGGGATGATCTGAATCAGCTCCCCTGGATTGGAGGGTAGCGGAAAGATTAAACCAGTTGGTTGGTGCAGGTGTCGGCGTTGTCCAGATTGATTATGTGTTGAAGCGGAg                                                                       >  2:1154352/1‑149 (MQ=255)
                                                                              aCTCATAACTATTTTAAGAAAAGAGGATGAAAGGTCATTGGGGATTAGCTCAATCAGCACCCCGGGAATGCAGGGGAGCGGCAAGATTAACCCAGTTCGTTCGGGCAGGTTTCGCCGTTTTCAAGATTGCATTAGTTGTGCAGCGTAGt                                                                      >  2:1039046/1‑149 (MQ=255)
                                                                              aCTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                                                                      >  2:416647/1‑149 (MQ=255)
                                                                                tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCt                                                                    >  1:825728/1‑149 (MQ=255)
                                                                                tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTCCTTAAGTTTTGCAGCGTAGTCt                                                                    >  2:246085/1‑149 (MQ=255)
                                                                                 cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                                   >  2:591235/1‑149 (MQ=255)
                                                                                   ggAATATTTTAAGAATATAGGATGAAAGGGCATTGGGGATTATCAGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                                                                 <  2:501831/149‑1 (MQ=255)
                                                                                      cTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa                                                              >  1:107098/1‑149 (MQ=255)
                                                                                         ttttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc                                                           >  1:912064/1‑149 (MQ=255)
                                                                                            tAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg                                                        >  1:193319/1‑149 (MQ=255)
                                                                                               gAATAGAGGATGAAAGGTCCTTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCa                                                     <  2:225555/149‑1 (MQ=255)
                                                                                                    gagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCt                                                >  1:33167/1‑149 (MQ=255)
                                                                                                      ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTATTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTCAGAAATACTGGTCAGAGCTTc                                              >  1:1120923/1‑149 (MQ=255)
                                                                                                       gATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTct                                             <  2:598676/149‑1 (MQ=255)
                                                                                                        aTGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctg                                            <  2:1150854/149‑1 (MQ=255)
                                                                                                             aGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTc                                       <  1:31961/149‑1 (MQ=255)
                                                                                                              gggcaagggggAGTAGCTGAATCACCTCCCCTGCCATGCCGGGGAGCGGCAAGATTAAACCACATCGTTCGGGCAGGCGGCGCCTTTTTCCAGATTGCTTAAGGTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCa                                      <  2:944201/142‑1 (MQ=255)
                                                                                                               gTCATTGGGGATTATCTGCATCAGCTCCCCTGGAATGCAGGGGAGCGGCACGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAg                                     <  1:665452/149‑1 (MQ=255)
                                                                                                                tCATTGGGGATTATCTGAATCAGCTCCCCTCGAATGCAGGGGAGCGACAAGATTAAACCAGTACGTTCGGGCAGTTTACGCATATTTCAAGATTGCTTAAAATTACCACCGTAATCTGAAAAATACTGGTAATAGCTTCTGCTCTCacg                                    >  2:772075/1‑147 (MQ=255)
                                                                                                                      gggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGcc                              <  1:954349/149‑1 (MQ=255)
                                                                                                                        gATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAACTACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTg                            >  2:835632/1‑149 (MQ=255)
                                                                                                                        gATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTg                            <  2:107098/149‑1 (MQ=255)
                                                                                                                                   tCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTaa                 >  1:737238/1‑149 (MQ=255)
                                                                                                                                     aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc               <  1:636944/149‑1 (MQ=255)
                                                                                                                                     aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGGAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc               >  1:1166235/1‑149 (MQ=255)
                                                                                                                                      gCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACa              >  2:1165762/1‑149 (MQ=255)
                                                                                                                                      gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGCTTGCTTATGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTGCTGCTGTCAGGAATGCCTGGTGCCCGGtttaca              >  2:949229/1‑144 (MQ=255)
                                                                                                                                      gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTATTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGATCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACa              >  2:615372/1‑149 (MQ=255)
                                                                                                                                         ccccTGGAATGCAGGGGCGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTATCCAGcc           >  2:1030903/1‑148 (MQ=255)
                                                                                                                                          cccGGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCAc          >  1:660325/1‑149 (MQ=255)
                                                                                                                                           ccTGGAAGGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACg         >  1:703544/1‑149 (MQ=255)
                                                                                                                                            cTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgct                                          >  1:1027773/1‑115 (MQ=255)
                                                                                                                                            cTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgct                                          <  2:1027773/115‑1 (MQ=255)
                                                                                                                                             tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGtt       <  1:180452/149‑1 (MQ=255)
                                                                                                                                              ggAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGTATGCCTGGTGCCCGGTAAACAGCACGTtg      >  2:721828/1‑149 (MQ=255)
                                                                                                                                              ggAATGAAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGGTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTtg      >  2:812953/1‑149 (MQ=255)
                                                                                                                                                 aTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTgg   <  2:408737/149‑1 (MQ=255)
                                                                                                                                                  tGCAGGGGAGCGGCAAGATTAACCCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGGAGTCTGAGAAATACTGGGCAGAGCTTCTGCTGGCCGGAATGCCTGGTGCCCGGTAAACAGCTCGTTGTggc  >  1:1061561/1‑149 (MQ=255)
                                                                                                                                                  tGCAGGGGAGCGGCAAGAGTAAACCAGTGCGTTCGGGCAGGTGTCGCCTTTTTCCAGATTGCTGAAGTTTTGCCGCGGAGTCGGAGAAATACTGGTCAGAGCTTCTGCGGTAAGGAATGCCTGGTGCCCGGTAAACAGCACGGTGTtgc  >  2:982010/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGC  >  NC_000913/1441690‑1441984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: