Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,706,114 G→A 8.0% V116V (GTG→GTA)  rsxA → SoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,706,1140GA 8.0% 134.2 / 3.2 50V116V (GTG→GTA) rsxASoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base A (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

CCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAACGCCTTGCTGTGGCTGATGTCCCG  >  NC_000913/1705987‑1706258
                                                                                                                               |                                                                                                                                                
cacTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTATGGTGGGGATTTTTTTGACGCATATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAat                                                                                                                             >  2:387245/3‑149 (MQ=255)
 ccTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAata                                                                                                                            >  2:933924/1‑149 (MQ=255)
 ccTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAata                                                                                                                            <  1:1110727/149‑1 (MQ=255)
  cTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGTTTTTTTTTCCGCTTATCACCACCCACTGTGCAGTACTCGGAGTGGGGTTGCTCAatat                                                                                                                           >  2:1047056/1‑149 (MQ=255)
  cTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTACTCGGCGTGGCGTTGCTGAatat                                                                                                                           >  2:1078454/1‑149 (MQ=255)
          tATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCg                                                                                                                   <  1:146612/149‑1 (MQ=255)
           tttCTGGGGAGAGCTGTGGTCGTGCAGTTCACCGAGACGGTGGTGGGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGCGAGTTTTTTGCCGCTTATCAGCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCgg                                                                                                                  <  1:92837/148‑1 (MQ=255)
           aTTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCgg                                                                                                                  >  2:638611/1‑149 (MQ=255)
            atCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCggg                                                                                                                 <  1:727423/148‑1 (MQ=255)
                 gTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTCCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACaa                                                                                                            >  2:967714/1‑149 (MQ=255)
                     ttGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCGGAATATCAATCTCGGGCACAATTTc                                                                                                        <  1:1047532/149‑1 (MQ=255)
                     ttGCTGTGGGCGTGCAGTTCACCGAGATGGTGGGGCGCAAAACCAGCCCGGGGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTc                                                                                                        <  2:106971/149‑1 (MQ=255)
                      tGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCt                                                                                                       <  1:199158/149‑1 (MQ=255)
                      tGCTGTGGTCGTGCAGTTCACCGAGATGGTGGAGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTAATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCt                                                                                                       <  1:704486/149‑1 (MQ=255)
                       gCTGTGGTCGTGCAGATCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCtt                                                                                                      <  1:58585/149‑1 (MQ=255)
                        cTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGGCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTg                                                                                                     >  1:709679/1‑149 (MQ=255)
                            ggTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGt                                                                                                 >  2:363102/1‑149 (MQ=255)
                                gTGCAGTTCACCGAGATGGTGGTGCGCACAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCACTCTCGGGCACAATTTCTTGCAGTCGgc                                                                                             >  1:42981/1‑149 (MQ=255)
                                gTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGgc                                                                                             >  1:481198/1‑149 (MQ=255)
                                  gCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCCCCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGgcgc                                                                                           >  1:288636/1‑149 (MQ=255)
                                      ttCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTa                                                                                       >  1:544768/1‑149 (MQ=255)
                                      ttCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCGCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTa                                                                                       <  1:982039/149‑1 (MQ=255)
                                        gaCCGAGATGGTGGTGCGCAAATCCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGGGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACg                                                                                     <  2:1046092/148‑1 (MQ=255)
                                          ccGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGTCACAATTTCTTGCAGTCTTCGCTGTACGGt                                                                                   <  2:599375/149‑1 (MQ=255)
                                          ccGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGt                                                                                   >  2:171218/1‑149 (MQ=255)
                                           cGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCCCCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGtt                                                                                  >  2:1021386/1‑149 (MQ=255)
                                           cGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGGGCTCGGCGTGGCGTTGCTGAATATCAATGTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGtt                                                                                  <  1:853348/149‑1 (MQ=255)
                                             agaTGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGtttt                                                                                >  1:797718/1‑149 (MQ=255)
                                               aTGGTGGTGCGCAAAACCAGCCCGGGGCTTTACCGTCTCCTGGGGAGTGTATTGCCGCTTATCACCACCAACTGGGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTc                                                                              <  2:641236/149‑1 (MQ=255)
                                                         gcAAAACCAGCCCGGGGCTTTACCGGCTGCTGGGGAGTTTTTTGCCGCTTATCACCACCAACTGTGCTGTGCTCGGCGGGGCGTTGCTGAATAGCAATCTCGGGCACAATTTCTTGCAGGCGGCGCTGTACGGTTTTTCCGCCGCTGTc                                                                    <  1:890570/149‑1 (MQ=255)
                                                           aaaaCCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCgg                                                                  <  2:979428/149‑1 (MQ=255)
                                                           aaaaCCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGGACGGTTTTTCCGCCGCTGTCgg                                                                  >  2:896714/1‑149 (MQ=255)
                                                           aaaaCCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCGCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCgg                                                                  <  1:1122569/149‑1 (MQ=255)
                                                               ccAGCCGGGTGCTTTCCCGGCTGCTGGGGATTTTGATGCCGCTTATGCCCACCTACTGTGCAGAGCGCGGCGTGGCGGTGCTGAAGATCAATCTGGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCTCCGCTGTCGGTTtc                                                              <  1:817922/149‑1 (MQ=255)
                                                               ccAGCCCGGTGCTTTACCGTCTGCTGGGGAGGTTTATTCCGCTTATCACCACCCACTCTGCAGGGCTCGGCGTGGCGGTGCGGCATATGCATCTCGGGCACCATTTGTTGCAGTCGGCGCGGGTCGGTGTTTCCGCCGCTGTCggggtc                                                              >  2:1059207/1‑145 (MQ=255)
                                                                cAGCCCGGTGCTTTACCGGCTGCTGGGGATTTTTTTGAGGCTTATCACCACCAACTGTGCAGTGCTCGTCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGTCGCTGTACGGTTTTTCGTCCGCTGTCGGTTtct                                                             <  2:463258/149‑1 (MQ=255)
                                                                   cccGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGc                                                          >  2:376647/1‑149 (MQ=255)
                                                                              aCCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTg                                               >  1:1000096/1‑149 (MQ=255)
                                                                                        ggggATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTcgccg                                     >  1:377696/1‑149 (MQ=255)
                                                                                           gATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCa                                  <  1:638611/149‑1 (MQ=255)
                                                                                            aTTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCTTTGCTGAATATCAATCTCGGGCAGAATTTCTTGCAGTCGGCGCTGTCCGGAGTTTCCGCCGCTGTCGGTTTCTCGCTGGGGATGGTGCTCTTCGCCTCCaa                                 >  1:660026/1‑148 (MQ=255)
                                                                                                    gCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAAc                         >  2:676603/1‑149 (MQ=255)
                                                                                                        cTTATCACCACCAACTGTGCAGTACTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAACGCCt                     <  1:1078454/149‑1 (MQ=255)
                                                                                                        cTTATCACCACCAACTGTGCAGAACTCGGCGTCGAGTTGCTGAATATCAATCTCGGGCACAAATTCTTGCAGTGGGCGCTGTACGGTTTTTCCGGCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAACGCCt                     <  1:1047056/149‑1 (MQ=255)
                                                                                                           aTCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCCTCCGCGAACGCCTTGc                  >  1:259337/1‑149 (MQ=255)
                                                                                                           aTCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCAATCCGCGAACGCCTTga                  >  1:1016418/1‑148 (MQ=255)
                                                                                                              acctccAACTGTGCAGTGCGCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGGCGCCATGCGCGAACGCCTTGCtgt               <  2:717776/149‑1 (MQ=255)
                                                                                                                caccaACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAACGCCTTGCTGTgg             >  1:928834/1‑149 (MQ=255)
                                                                                                                     aCTGTGCAGTGCGCGGCGTGGCGGTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCGGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAACGCCTTGCTGTGGCTGAt        <  1:606211/149‑1 (MQ=255)
                                                                                                                           cAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAACGCCTTGCTGTGGCTGATGTCCCg  <  2:288636/149‑1 (MQ=255)
                                                                                                                               |                                                                                                                                                
CCCTGGCATTTATTCTGGTGATTGCTGTGGTCGTGCAGTTCACCGAGATGGTGGTGCGCAAAACCAGCCCGGTGCTTTACCGTCTGCTGGGGATTTTTTTGCCGCTTATCACCACCAACTGTGCAGTGCTCGGCGTGGCGTTGCTGAATATCAATCTCGGGCACAATTTCTTGCAGTCGGCGCTGTACGGTTTTTCCGCCGCTGTCGGTTTCTCGCTGGTGATGGTGCTCTTCGCCGCCATCCGCGAACGCCTTGCTGTGGCTGATGTCCCG  >  NC_000913/1705987‑1706258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑