Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,765,157	T→A	11.2%	intergenic (‑35/+65)	ydiH ← / ← menI	uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,765,157	0	T	A	11.2%	111.9 / 5.9	45	intergenic (‑35/+65)	ydiH/menI	uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base T (25/15);  new base A (3/2);  total (28/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

CAAGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGT  >  NC_000913/1765014‑1765301                                                                                                                                                |                                                                                                                                                 cAAGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAacggt                                                                                                                                             >  2:1164330/1‑146 (MQ=255)       cTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTa                                                                                                                                       <  1:399820/149‑1 (MQ=255)              tttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCa                                                                                                                                <  2:1178909/149‑1 (MQ=255)               ttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAACGGTGGTATACTGTGCAg                                                                                                                               <  1:1164330/149‑1 (MQ=255)                  aGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGGTGGGTGGGATCAAGTTGAGTAGACATAGCAACCTCGCTTTTAGACAAGACCTGCTCAGTATACCACCGTTTACTGTGCAGATa                                                                                                                            >  1:203262/1‑149 (MQ=255)                       tttGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGAc                                                                                                                       >  1:533169/1‑149 (MQ=255)                                        gTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTc                                                                                                      >  1:246556/1‑149 (MQ=255)                                         tGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTca                                                                                                     >  1:747366/1‑149 (MQ=255)                                         tGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTca                                                                                                     <  2:284378/149‑1 (MQ=255)                                                ttGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACtttt                                                                                              <  2:67274/149‑1 (MQ=255)                                                  tctcagttCACGACGTAAAAATTCAATGGCCAGCTGGGGGGGATCAATTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGGCACACTTTTCt                                                                                            <  2:777890/142‑1 (MQ=255)                                                   ctctGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTg                                                                                           <  2:747366/149‑1 (MQ=255)                                                   ctctGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTg                                                                                           <  1:509293/149‑1 (MQ=255)                                                              gCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTc                                                                                >  2:1023442/1‑149 (MQ=255)                                                                gTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTcac                                                                              <  2:199765/149‑1 (MQ=255)                                                                   aaaaTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACaaa                                                                           >  2:906402/1‑149 (MQ=255)                                                                      aTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATg                                                                        <  2:14617/149‑1 (MQ=255)                                                                       ttCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATgg                                                                       <  1:679898/149‑1 (MQ=255)                                                                         cAATGCCCAGCGGGGTGGGATCCCGATGATTACTCATATCATCCTCGCGTATAGACAAGAACAGCACAGTATACCACCGGTTACTCTGCAGATACTGCCCAAAAGCAATAGGCGTCACACTTTTCTGCTGACAACGTCACAAAATGGCg                                                                     <  2:765914/149‑1 (MQ=255)                                                                                      ggtgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTg                                                        >  2:738702/1‑149 (MQ=255)                                                                                           gATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACgca                                                   >  1:133702/1‑147 (MQ=255)                                                                                           gATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGaa                                                   <  1:141906/149‑1 (MQ=255)                                                                                             tCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACa                                                 >  2:723446/1‑149 (MQ=255)                                                                                             tCAAGTTGAGTAGACAAAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACAGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACATTTCTGGTGACAACGTCACAAAATCGCGGTCGTCAATCGTGACGaaaa                                                 >  1:698208/1‑147 (MQ=255)                                                                                                aGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACAAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGca                                              >  1:997778/1‑149 (MQ=255)                                                                                                 gTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcac                                             >  1:1126812/1‑149 (MQ=255)                                                                                                    gAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACaaa                                          >  1:1175905/1‑149 (MQ=255)                                                                                                     tgTAGTCATAGCATGCTCGCTTTTAGACAAGACCTGCACAGTATACCAACGTTTACTGTGCAGATAATGTCCAAAAGCAATATGCGTCAGACTTTTCTGGTGACATCGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACTAAc                                         <  2:1142661/148‑1 (MQ=255)                                                                                                      gTAGAAATAGCATCCTCGCTTTTAGACAAGACCTGCTCAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACg                                        <  2:297985/149‑1 (MQ=255)                                                                                                       tAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACCACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGc                                       >  1:437898/1‑149 (MQ=255)                                                                                                                     tCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGCACAGCACAAACGCCCTTTCTCATCGaa                         >  1:254706/1‑149 (MQ=255)                                                                                                                       gCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAACCGCCCTTTCTCATCGAAGa                       >  2:957327/1‑149 (MQ=255)                                                                                                                       gCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGa                       >  1:1023809/1‑149 (MQ=255)                                                                                                                        cTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAACCGCCCTTTCTCGTCGAAGAt                      >  1:1099566/1‑149 (MQ=255)                                                                                                                               aCAATACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCACTTTCTCATCGAAGATTTCAATc               >  1:259121/1‑149 (MQ=255)                                                                                                                               aCAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATc               >  2:847387/1‑149 (MQ=255)                                                                                                                                cattatcTGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                             <  1:973635/128‑1 (MQ=38)                                                                                                                                cattatcTGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                             >  2:973635/7‑134 (MQ=38)                                                                                                                                  aGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGc            <  2:997778/149‑1 (MQ=255)                                                                                                                                      cTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAgc        >  2:252686/1‑148 (MQ=255)                                                                                                                                      cTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGa        >  1:629502/1‑149 (MQ=255)                                                                                                                                      cTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGa        >  1:276040/1‑149 (MQ=255)                                                                                                                                       tGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGAc       >  2:144718/1‑149 (MQ=255)                                                                                                                                       tGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGATGATTTCAATCTGCCAGAc       >  2:7009/1‑149 (MQ=255)                                                                                                                                            aGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGCCCTGGt  >  1:1039214/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                 CAAGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGT  >  NC_000913/1765014‑1765301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑