Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,904,212 G→A intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,904,2120GA95.1% 261.4 / NA 104intergenic (+838/+535)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (48/49);  minor base C (5/0);  total (55/49)
Fisher's exact test for biased strand distribution p-value = 5.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTTT  >  NC_000913/2904069‑2904357
                                                                                                                                               |                                                                                                                                                 
ggCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATTGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCgg                                                                                                                                              <  2:33186/149‑1 (MQ=255)
ggCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCgg                                                                                                                                              <  1:1028318/149‑1 (MQ=255)
ggCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCgg                                                                                                                                              <  1:634329/149‑1 (MQ=255)
ggCTCCGGTTTATCCCCGCTGGCGAGGGGAACACCTGTGTTCGCAAATCTATCGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGCGAACACGGGAGCACTGAAAACAAAGCAGTGAATAAGCTCGGGTAATACCCGCTGACGCgg                                                                                                                                              >  1:230247/1‑149 (MQ=255)
 gCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGTGCGAACGGAATACAAAGCCGAGGATCTGATCGGTTTATACCCGCTGACGCggg                                                                                                                                             >  2:679240/1‑149 (MQ=255)
  atCCGGTTTATCCCCGCTGTCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGTGAACTCGGGCGCACGGAATACATAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCgggg                                                                                                                                            <  1:447887/148‑1 (MQ=255)
      ggTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAaca                                                                                                                                        <  2:596269/149‑1 (MQ=255)
      ggTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAaca                                                                                                                                        <  2:771333/149‑1 (MQ=255)
        tttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACt                                                                                                                                      <  1:1068105/149‑1 (MQ=255)
        tttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACt                                                                                                                                      <  2:746741/149‑1 (MQ=255)
         ttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTg                                                                                                                                     <  2:430651/149‑1 (MQ=255)
         ttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTg                                                                                                                                     <  1:1131354/149‑1 (MQ=255)
             ccccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTAGGGACTATTGCGATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctc                                                                                                                                 >  1:1023479/1‑149 (MQ=255)
              cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctct                                                                                                                                >  2:273858/1‑149 (MQ=255)
              cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctct                                                                                                                                <  2:656407/149‑1 (MQ=255)
              cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctct                                                                                                                                <  1:480247/149‑1 (MQ=255)
              cccGCGGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctct                                                                                                                                <  2:670425/149‑1 (MQ=255)
               ccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTg                                                                                                                               >  2:1340516/1‑149 (MQ=255)
               ccGCTGGCGCGGGGAACACATTATTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGAGAAAGGAATACAAATAAATGTATGTGGTCTGTTGATCCCCGCTGTCGAGGGGAAGAATGGatcag                                                                                                                               >  2:997537/1‑147 (MQ=255)
                 gCTGGCGCGGGGAACACAAGATTTAGCTAAGATATGGACGATTGATAGTAGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCAAGGAATTAAAATACGTGTATATGGGCGGTTTATCCCCGCTGGGGGGGGGAGGACTGGCTCTggg                                                                                                                             >  2:205111/1‑147 (MQ=255)
                  cTGGCGCGGGGAACACCTGTTTTCTAAAATCTATGTACTATTGCTATTCGGTTTATCCCCGCTGGCGCGTGGAACAAGGGCGCCCGGAATACAAAGACGTGTATCTGCTCGGTTTATCCCCGCTGACGCTGGGAACACTGGCTCTTCaa                                                                                                                            <  2:1233765/149‑1 (MQ=255)
                    ggCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAca                                                                                                                          <  2:359911/149‑1 (MQ=255)
                    ggCGCGGGGAACAACTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGCCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCGCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAca                                                                                                                          <  2:791642/149‑1 (MQ=255)
                       gctgggAACACCTGTTTTCGCAAATCTATGGACTCATGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagca                                                                                                                       <  1:1425185/146‑1 (MQ=255)
                       gcgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCAGCTGACGCGGGGAACACTGGCTCTGCAAcagca                                                                                                                       >  1:1435409/1‑149 (MQ=255)
                           ggAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAc                                                                                                                   <  2:193013/149‑1 (MQ=255)
                               cacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATATGCTCGGTTTATCCCCGCTGACGAGGGGAACACTGGCTCTGCAACAGCAGCACCCAt                                                                                                               >  2:485530/1‑149 (MQ=255)
                                acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGTCTCTGCAACAGCAGCACCCATg                                                                                                              >  1:1306247/1‑149 (MQ=255)
                                acCTGTTATCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGAATCTGCTCGGTTTATACCCGCTGACGCGCGGAACACTGGCTCTCCAACAGCAGCACCCAtc                                                                                                              >  1:769425/1‑148 (MQ=255)
                                  cTGTTTTCGCAAATCTATGGACTATTGCTATTCTGTTTATCCCCGCTGGCGCGGGGAACACGTGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGCGAACACTGGCTCACCAACAGCAGCACCCAAGAc                                                                                                            >  2:822298/1‑149 (MQ=255)
                                  cTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCGGCACCCATGAc                                                                                                            <  2:313881/149‑1 (MQ=255)
                                    gTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCggg                                                                                                                                             >  2:1334066/1‑114 (MQ=255)
                                    gTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCggg                                                                                                                                             <  1:1334066/114‑1 (MQ=255)
                                    gTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCa                                                                                                          >  1:1306720/1‑149 (MQ=255)
                                     ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCTGCACCCATGACCAc                                                                                                         >  1:1062950/1‑149 (MQ=255)
                                     ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCAc                                                                                                         >  2:228039/1‑149 (MQ=255)
                                          gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCgg                                                                                                    >  2:102543/1‑149 (MQ=255)
                                           cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACCCGGGCGCACGGAATACAAAGCCGTGTAACTGCTCGGTTTATCCCCGCTGAAGAGGGGAACACTTGCTCTGCAACAGCATCCCCCATTACCCCGTCGGt                                                                                                   >  2:1107960/1‑149 (MQ=255)
                                           cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGTCGCACGGAAAACAAAGCCGTGTATCTGCTCGGTATATACACGCTCACGCGGGGAACACTGGCTCTGAAACAGCAGAACCCATGACAACGtagct                                                                                                   >  1:18744/1‑145 (MQ=255)
                                           cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                                   >  2:432717/1‑149 (MQ=255)
                                            aaaTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGTCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGtt                                                                                                  <  1:1070308/149‑1 (MQ=255)
                                            aaaTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGtt                                                                                                  <  2:347019/149‑1 (MQ=255)
                                            aaaTCTATGGACTATCGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGtt                                                                                                  <  1:122156/149‑1 (MQ=255)
                                              aTCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTa                                                                                                >  1:1190172/1‑149 (MQ=255)
                                               tCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTAt                                                                                               <  1:974852/149‑1 (MQ=255)
                                                  aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGACCACTGGCTCTGCAACAGCAGCCCCCATGACCACGTCGGTTTATccc                                                                                            >  1:518107/1‑149 (MQ=255)
                                                  aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATccc                                                                                            >  1:712170/1‑149 (MQ=255)
                                                     gagtcttGCTATTCGGCTTATCCCCCCTTGCGCGGGGAACACGGGCGCCCGGAATCCAACGCCGTGTATCTGCTCGGCTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCTCGTCGGTTTATCCCCGc                                                                                         <  2:1180488/144‑1 (MQ=255)
                                                      aCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGATCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACCGCAGCACCCATGACCACGTCGGTTTATCCCCGCt                                                                                        >  1:1098890/1‑149 (MQ=255)
                                                       cTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCACGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTg                                                                                       >  2:3602/1‑149 (MQ=255)
                                                        tATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCACCAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTgg                                                                                      >  2:241013/1‑149 (MQ=255)
                                                         aTTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCCCTGgc                                                                                     >  2:808507/1‑149 (MQ=255)
                                                             cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCgg                                                                                 >  1:606781/1‑149 (MQ=255)
                                                              tATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg                                                                                <  1:654417/149‑1 (MQ=255)
                                                              tATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg                                                                                <  1:1048573/149‑1 (MQ=255)
                                                                ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGa                                                                              >  2:1337470/1‑149 (MQ=255)
                                                                ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTGATCCCCGCTGCCGCGGGGAACACTGGCTCTGCCCCCGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGa                                                                              >  2:822166/1‑149 (MQ=255)
                                                                   ggTTTTTCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGCCCACGTCGGTTTATCCCCGCGGGCGCGGGGaccc                                                                           >  1:591176/1‑146 (MQ=255)
                                                                      ttAGCCCCGCGGGCGCGGGGAACACGGGCGCACGGCATACAACTCCGTGTATCTGCTCGGGATATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGa                                                                        <  1:798947/149‑1 (MQ=255)
                                                                        aTCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTGTGCCACCGCGGCACACAGGACCACGTCGGTTTATCACCGCTGGAGCGGGGAACACGaaa                                                                      >  2:944439/1‑149 (MQ=255)
                                                                        aTCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGAATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGaaa                                                                      <  2:603533/149‑1 (MQ=255)
                                                                           cccGCTGGCGCGGGGACCACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGCCCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGc                                                                   >  1:1095675/1‑149 (MQ=255)
                                                                             cGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTg                                                                 <  1:387441/149‑1 (MQ=255)
                                                                               cTGGCGCTGGGAACACGGGCTCACGGAATACAAAGCGGTTTATCTGCTCGGTTTATCCCCGCTGAAGCGGGGAACACTGGCTATTGAAAAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGt                                                               <  1:1141930/149‑1 (MQ=255)
                                                                                tGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTg                                                              >  2:578504/1‑149 (MQ=255)
                                                                                    gcgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCg                                                          >  1:920197/1‑149 (MQ=255)
                                                                                       gggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTTGTGAGCGTTa                                                       <  1:382552/149‑1 (MQ=255)
                                                                                        ggAACACGGGCTCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAACAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTaa                                                      <  2:464502/149‑1 (MQ=255)
                                                                                            cacGGGCGCACGGAATACGAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATTACCACGTCGTTTTATCACAGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc                                                  >  1:839428/1‑149 (MQ=255)
                                                                                            cacGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc                                                  >  1:841284/1‑149 (MQ=255)
                                                                                             acGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCACCACCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGGACACGAAATGCTGGTGAGCGTTAATGCCg                                                 >  1:1306759/1‑149 (MQ=255)
                                                                                                 gcgcACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCaaa                                             <  1:1340516/149‑1 (MQ=255)
                                                                                                     aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCATAcaca                                         >  1:798936/1‑149 (MQ=255)
                                                                                                     aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAAcaca                                         <  1:102543/149‑1 (MQ=255)
                                                                                                      cGGAATACAATGCGGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                        <  1:1164368/149‑1 (MQ=255)
                                                                                                      cGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                        >  2:897098/1‑149 (MQ=255)
                                                                                                      cGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                        <  1:753702/149‑1 (MQ=255)
                                                                                                       ggAATACAACGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAgg                                       <  1:610037/149‑1 (MQ=255)
                                                                                                        gAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTAACCCCGCGGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt                                      >  2:459936/1‑149 (MQ=255)
                                                                                                        gAATAAAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAACCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt                                      <  2:321610/149‑1 (MQ=255)
                                                                                                            aCAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTAt                                  >  1:1262209/1‑149 (MQ=255)
                                                                                                              aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTTATGCCGCAAACACAGGTTTATcc                                >  1:83039/1‑149 (MQ=255)
                                                                                                              aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcc                                >  1:1370169/1‑149 (MQ=255)
                                                                                                              aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcc                                >  1:986663/1‑149 (MQ=255)
                                                                                                                aGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcccc                              <  2:606781/149‑1 (MQ=255)
                                                                                                                  ccGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGc                            <  2:305663/149‑1 (MQ=255)
                                                                                                                          cTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCgg                    <  1:273858/149‑1 (MQ=255)
                                                                                                                           ggCTCGGTGAATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGGCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCggg                   <  2:504201/148‑1 (MQ=255)
                                                                                                                            gCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCGCCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCACACGCTATGCTGGTGAGCGGTGATGCCGCAACCACAGGTTTATACCCGCTGGGGCgggg                  >  2:817582/1‑149 (MQ=255)
                                                                                                                             cTCGGTTTATCCCCGCTGACGCGGGGACCACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACCGGTTTATCCCCGCTGGCGGGGGGa                 >  1:945309/1‑149 (MQ=255)
                                                                                                                                ggTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAATCACAGGTTTATCCCCGCTGGCGCGGGGAaca              >  1:917257/1‑149 (MQ=255)
                                                                                                                                     aTCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTa         <  1:553556/149‑1 (MQ=255)
                                                                                                                                      tCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAATCACAGGTTTATCCCCGCTGGCGCGGGGAACACATTAc        <  2:712170/149‑1 (MQ=255)
                                                                                                                                        cccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGGCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGc      <  1:1300954/149‑1 (MQ=255)
                                                                                                                                         ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGcc     >  2:237647/1‑149 (MQ=255)
                                                                                                                                         ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGAAGAAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTAGGcc     >  1:688638/1‑149 (MQ=255)
                                                                                                                                          cGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGTCCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGACGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCt    >  1:1441364/1‑149 (MQ=255)
                                                                                                                                           gCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGTTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGAGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCtt   >  2:662148/1‑149 (MQ=255)
                                                                                                                                           gCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAACTGCTGGTGAGCGTTAATGCCGCAAACACAGGGTTATCCCCGCTGGCGCGGGGAACACATTACGCCtt   <  1:1107960/149‑1 (MQ=255)
                                                                                                                                            cTGACGCGGGGCACACTGGCGCGGCAACAGCAGCACCCTTGACCACGGCGGGTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCttt  <  1:822166/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTTT  >  NC_000913/2904069‑2904357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: