Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,189,448 C→T Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,189,4480CT90.9% 357.2 / 24.1 124Q1367* (CAG→TAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (1/10);  new base T (49/61);  total (52/72)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01

Only 100 of 126 total aligned reads displayed.

CGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC  >  NC_000913/4189312‑4189583
                                                                                                                                        |                                                                                                                                       
cgcgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                                                                      >  1:1190082/1‑140 (MQ=255)
cgcgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                                                                      >  2:396585/1‑140 (MQ=255)
  cgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCACACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                    >  1:109131/1‑140 (MQ=255)
  cgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACTTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                    >  2:342845/1‑140 (MQ=255)
  cgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                    >  2:252655/1‑140 (MQ=255)
  cgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                    >  1:636246/1‑140 (MQ=255)
     cATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGt                                                                                                                                 <  2:1202413/140‑1 (MQ=255)
     cATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGt                                                                                                                                 <  2:618815/140‑1 (MQ=255)
        cGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                                                              <  2:1266088/140‑1 (MQ=255)
        cGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                                                              <  2:1263567/140‑1 (MQ=255)
        cGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                                                              <  2:243047/140‑1 (MQ=255)
        cGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                                                              <  2:243826/140‑1 (MQ=255)
        cGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                                                              <  2:77511/140‑1 (MQ=255)
        cGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                                                              <  2:740836/140‑1 (MQ=255)
          ttCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCg                                                                                                                            <  1:480860/140‑1 (MQ=255)
          ttCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCg                                                                                                                            <  1:481232/140‑1 (MQ=255)
             cAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGACAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCg                                                                                                                         <  2:1235414/140‑1 (MQ=255)
              aGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGc                                                                                                                        <  1:977906/140‑1 (MQ=255)
              aGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGc                                                                                                                        <  1:978254/140‑1 (MQ=255)
               ggAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGcc                                                                                                                       >  1:1186628/1‑140 (MQ=255)
               ggAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGcc                                                                                                                       >  1:1186906/1‑140 (MQ=255)
                   aCCACTCGCGTGCTGACCGAAGCAGCCGTTGCGTGCAAACGCGACGAACTGCGCGGCCTGATAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgc                                                                                                                   >  2:470244/1‑140 (MQ=255)
                   aCCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgc                                                                                                                   >  2:1656780/1‑140 (MQ=255)
                   aCCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgc                                                                                                                   >  2:868499/1‑140 (MQ=255)
                    ccACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgct                                                                                                                  <  1:739222/140‑1 (MQ=255)
                    ccACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGtgct                                                                                                                  <  1:699065/140‑1 (MQ=255)
                     cACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgctg                                                                                                                 >  2:1191643/1‑140 (MQ=255)
                     cACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgctg                                                                                                                 >  2:294619/1‑140 (MQ=255)
                     cACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgctg                                                                                                                 >  1:1116159/1‑140 (MQ=255)
                     cACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtgctg                                                                                                                 >  1:1659373/1‑140 (MQ=255)
                     cACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGagctg                                                                                                                 >  2:1192201/1‑140 (MQ=255)
                          gcgTGCTGACCGAAGCAGCCGTTGCGGGCACACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGAGGGTCGTCTGATCCCGGCCGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCgggg                                                                                                            >  2:239734/1‑139 (MQ=255)
                          gcgTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGt                                                                                                            <  1:545359/140‑1 (MQ=255)
                               ccgACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGc                                                                                                       <  1:714461/138‑1 (MQ=255)
                                  aCCGAAGCAGCCGTTGCGGGCAAACGAGACGAACTTCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACAAGGATCGTATGAGTCGCCGTGCTTCGGGTGAAGCTcc                                                                                                    >  2:1352362/1‑140 (MQ=255)
                                       agcagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTg                                                                                               <  1:1233776/140‑1 (MQ=255)
                                            ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCg                                                                                          >  2:88835/1‑140 (MQ=255)
                                            ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCg                                                                                          <  2:109187/140‑1 (MQ=255)
                                            ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCg                                                                                          >  1:1601354/1‑140 (MQ=255)
                                            ccGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCg                                                                                          <  2:1159809/140‑1 (MQ=255)
                                              gTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCa                                                                                        <  2:53357/140‑1 (MQ=255)
                                                 gCGGGCAAACGCGACGAACTGCGCGGCCTGCAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGt                                                                                     <  1:473913/140‑1 (MQ=255)
                                                   gggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGa                                                                                   <  2:6921/140‑1 (MQ=255)
                                                        aaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCa                                                                              <  1:1571143/140‑1 (MQ=255)
                                                            cgacgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCagaag                                                                          <  1:1300303/140‑1 (MQ=255)
                                                                   cTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTCGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATc                                                                   <  2:630669/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:49025/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:151473/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:924772/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:539182/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:924557/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:708830/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:387392/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:98503/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:295685/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:379033/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:1147099/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGTTCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  1:368571/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  1:368467/140‑1 (MQ=255)
                                                                    tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCAGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCt                                                                  <  2:1367598/140‑1 (MQ=255)
                                                                      cgcgGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGc                                                                >  1:1250643/1‑140 (MQ=255)
                                                                      cgcgGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGc                                                                <  2:1189754/140‑1 (MQ=255)
                                                                       gcgGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCTCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCa                                                                                        <  2:1348299/115‑1 (MQ=255)
                                                                              gAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTgg                                                        <  2:125671/140‑1 (MQ=255)
                                                                              gAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTgg                                                        <  2:125412/140‑1 (MQ=255)
                                                                              gAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGAGACTGCAGAAGACGCATCTGCCAGCCTgg                                                        >  1:1778083/1‑140 (MQ=255)
                                                                                     aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAct                                                 >  1:1032291/1‑140 (MQ=255)
                                                                                     aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAct                                                 >  1:1562182/1‑140 (MQ=255)
                                                                                     aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAct                                                 >  1:1609074/1‑140 (MQ=255)
                                                                                     aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAct                                                 >  1:410611/1‑140 (MQ=255)
                                                                                     aaCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAct                                                 >  2:1749074/1‑140 (MQ=255)
                                                                                     aaCGTTAACGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAAct                                                 >  2:1749019/1‑140 (MQ=255)
                                                                                        gTTATCGTGGGTCGTCTGTTCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct                                              <  1:947024/140‑1 (MQ=255)
                                                                                        gTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct                                              <  1:947012/140‑1 (MQ=255)
                                                                                        gTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct                                              <  2:457961/140‑1 (MQ=255)
                                                                                        gTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct                                              <  2:1208675/140‑1 (MQ=255)
                                                                                        gTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgct                                              <  1:1676770/140‑1 (MQ=255)
                                                                                         ttATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAActgctg                                             >  2:365419/1‑140 (MQ=255)
                                                                                            tCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAAc                                          >  1:1182470/1‑140 (MQ=255)
                                                                                             cGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACg                                         <  2:1197821/140‑1 (MQ=255)
                                                                                             cGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACg                                         <  1:1311292/140‑1 (MQ=255)
                                                                                                  gtcgtcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGt                                    <  2:1143202/140‑1 (MQ=255)
                                                                                                      tcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTgg                                >  2:881023/1‑140 (MQ=255)
                                                                                                      tcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGAAGGTCTgg                                >  2:278909/1‑140 (MQ=255)
                                                                                                          aTCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCgg                            >  2:265242/1‑140 (MQ=255)
                                                                                                           tCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGt                           >  2:485644/1‑140 (MQ=255)
                                                                                                             ccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCACCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTc                         <  2:1601641/140‑1 (MQ=255)
                                                                                                               ggCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCTGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTg                       >  2:838158/1‑140 (MQ=255)
                                                                                                               ggCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTg                       >  2:837846/1‑140 (MQ=255)
                                                                                                               ggCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTg                       >  1:1536810/1‑140 (MQ=255)
                                                                                                                gCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGa                      >  1:1098693/1‑140 (MQ=255)
                                                                                                                  cggcaCCGGCTCCGCGCACCACACGGAGCGTCCGCGACGCCGAGCAGCGGGAGAAGCTCCGGCGGCACCGCAGCTGCCCGCAGAAGACGCAACTGCCAGCCTGGCAGAACAGCCGACCGCAGGGCTGGGCGGTTCAGATa                    <  2:1592896/136‑1 (MQ=255)
                                                                                                                  cggTACCGGTTTCGCGTACCACTAGGCTCGTATGCGTCGCCGTGCTGCGGGAGCCGCTCCGGCTGCACCGCAGGTGACTGCAGACGACGCATCTGCCAGCCTGGCAGAACTGCTGCACGCAGGTCTGGGCGGTTCTGATa                    <  2:1594627/139‑1 (MQ=255)
                                                                                                                  aGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATa                    <  1:1753418/140‑1 (MQ=255)
                                                                                                                  aGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATa                    <  2:1299609/140‑1 (MQ=255)
                                                                                                                          gTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTaa            >  2:885824/1‑140 (MQ=255)
                                                                                                                               gcgTACCACTAGGATCGAATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGtt       >  2:147599/1‑140 (MQ=255)
                                                                                                                                  tACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAAt    >  1:1478219/1‑140 (MQ=255)
                                                                                                                                    ccactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATcc  >  1:1745156/1‑140 (MQ=255)
                                                                                                                                    ccactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATcc  >  1:1745712/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC  >  NC_000913/4189312‑4189583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑