Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,074,894 G→T 6.1% intergenic (+14/+26) rutR → / ← putA rut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,074,8940GT 6.1% 1008.9 / 35.2 280intergenic (+14/+26)rutR/putArut operon transcriptional repressor for/fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (118/144);  new base T (9/9);  total (127/153)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAA  >  NC_000913/1074745‑1075043
                                                                                                                                                     |                                                                                                                                                     
ttCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGgaggag                                                                                                                                                       <  2:1238499/150‑1 (MQ=255)
 tCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGaggagg                                                                                                                                                      >  1:2717736/1‑150 (MQ=255)
 tCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGaggagg                                                                                                                                                      <  1:764915/150‑1 (MQ=255)
  ccACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGt                                                                                                                                                     <  2:2588649/150‑1 (MQ=255)
      tCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTa                                                                                                                                                 <  1:477985/150‑1 (MQ=255)
       cAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTaa                                                                                                                                                <  1:3014096/150‑1 (MQ=255)
         aCATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACa                                                                                                                                              <  1:5058672/150‑1 (MQ=255)
           aTTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTACAATCAAACGGTTGAAAACGAGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtc                                                                                                                                            <  1:439840/150‑1 (MQ=255)
              aCGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctc                                                                                                                                         >  2:4105424/1‑150 (MQ=255)
               cGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGTGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctcc                                                                                                                                        >  1:2864722/1‑150 (MQ=255)
               cGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCtcctcc                                                                                                                                        <  1:3030456/150‑1 (MQ=255)
               cGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctcc                                                                                                                                        <  2:3091786/150‑1 (MQ=255)
               cGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACAtcctcc                                                                                                                                        >  1:3443043/1‑150 (MQ=255)
                gCCGATTTCGCCCCTCAGGTGGAGGCGGTGCCAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCg                                                                                                                                       <  2:3971834/150‑1 (MQ=255)
                gCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCg                                                                                                                                       <  1:425649/150‑1 (MQ=255)
                   gATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCt                                                                                                                                    <  2:546098/150‑1 (MQ=255)
                         gCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGACGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGATGTTACAACCTCCTCCGGcatcttta                                                                                                                              <  2:2087847/150‑8 (MQ=255)
                          ccccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTCCCTGtt                                                                                                                             >  2:460838/1‑150 (MQ=255)
                          ccccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGtt                                                                                                                             <  2:898286/150‑1 (MQ=255)
                           cccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGttt                                                                                                                            >  2:1612935/1‑150 (MQ=255)
                           cccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGttt                                                                                                                            >  2:3835448/1‑150 (MQ=255)
                           cccTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGttt                                                                                                                            >  2:3930360/1‑150 (MQ=255)
                              tCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAAc                                                                                                                         >  2:277645/1‑150 (MQ=255)
                              tCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAAc                                                                                                                         <  2:4956361/150‑1 (MQ=255)
                              tCAGGTGGAGGCGGTGACAGGCGCGACGGTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAAc                                                                                                                         >  1:1152187/1‑150 (MQ=255)
                                  gTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCtat                                                                                                                     >  2:2906546/1‑150 (MQ=255)
                                   tGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCtata                                                                                                                    <  2:2157900/150‑1 (MQ=255)
                                    ggAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAg                                                                                                                   <  2:1109198/150‑1 (MQ=255)
                                    ggAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTAGTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAg                                                                                                                   <  1:927348/150‑1 (MQ=255)
                                        gCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCAt                                                                                                               <  2:4423494/150‑1 (MQ=255)
                                            tGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAg                                                                                                           >  2:8049/1‑150 (MQ=255)
                                             gACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGc                                                                                                          <  1:121242/150‑1 (MQ=255)
                                                aGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTgg                                                                                                       <  1:377341/150‑1 (MQ=255)
                                                aGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTgg                                                                                                       >  2:1459117/1‑150 (MQ=255)
                                                 ggCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGc                                                                                                      <  2:5012011/150‑1 (MQ=255)
                                                 ggCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGc                                                                                                      <  2:3309291/150‑1 (MQ=255)
                                                  gcgcGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCg                                                                                                     >  2:1966064/1‑150 (MQ=255)
                                                      gACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTAc                                                                                                 >  1:3925949/1‑150 (MQ=255)
                                                                gATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggc                                                                                       <  2:3719406/150‑1 (MQ=255)
                                                                   gAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagc                                                                                    <  1:4646822/150‑1 (MQ=255)
                                                                    aGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcggcagcg                                                                                   <  2:661058/150‑1 (MQ=255)
                                                                       tATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTa                                                                                >  1:659659/1‑150 (MQ=255)
                                                                        aTTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAt                                                                               >  2:2300043/1‑150 (MQ=255)
                                                                        aTTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAt                                                                               <  2:3414570/150‑1 (MQ=255)
                                                                        aTTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAt                                                                               <  2:1536148/150‑1 (MQ=255)
                                                                         tttttCAATCAACCGGTTGAAAACGTGCAGCGGTTTTTTTTTGTGGGGATTCGACCACTTTAAAGTTGCCGGAGGTGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAtt                                                                              <  1:4040130/150‑1 (MQ=255)
                                                                         tttttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAtt                                                                              <  2:2230882/150‑1 (MQ=255)
                                                                           tttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca                                                                            >  1:237525/1‑150 (MQ=255)
                                                                           tttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTca                                                                            <  1:4081728/150‑1 (MQ=255)
                                                                            ttCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTcac                                                                           >  1:2459093/1‑150 (MQ=255)
                                                                               actcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTACAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACt                                                                        <  2:880610/148‑1 (MQ=255)
                                                                                 tcaaACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTCTTCACACTCa                                                                      >  1:4841418/1‑150 (MQ=255)
                                                                                   aaaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGc                                                                    <  2:4488219/150‑1 (MQ=255)
                                                                                    aaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCg                                                                   >  1:3707082/1‑150 (MQ=255)
                                                                                    aaCGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCg                                                                   >  1:4446156/1‑150 (MQ=255)
                                                                                      cGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGaa                                                                 <  1:3591875/150‑1 (MQ=255)
                                                                                         ttGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTCCCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGc                                                              >  1:4530402/1‑150 (MQ=255)
                                                                                           gAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTc                                                            <  2:238490/150‑1 (MQ=255)
                                                                                           gAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTc                                                            <  2:4708723/150‑1 (MQ=255)
                                                                                           gAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTc                                                            <  1:4520056/150‑1 (MQ=255)
                                                                                           gAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGATTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTc                                                            <  1:2625548/150‑1 (MQ=255)
                                                                                             aaaCGTGCAGCGGATTATTATTGAGTGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGa                                                          <  2:2864722/150‑1 (MQ=255)
                                                                                             aaaCGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGa                                                          <  1:1459117/150‑1 (MQ=255)
                                                                                             aaaCGTGCAGCGGATTAGTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACGCTCAGCGAACGCTCGa                                                          <  2:1031171/150‑1 (MQ=255)
                                                                                                 gTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGAtata                                                      <  2:4840263/150‑1 (MQ=255)
                                                                                                     aGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGc                                                  >  2:4095771/1‑150 (MQ=255)
                                                                                                     aGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGc                                                  <  2:354593/150‑1 (MQ=255)
                                                                                                              ttattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGa                                         <  2:852406/150‑1 (MQ=255)
                                                                                                               tattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGaa                                        <  2:4176648/150‑1 (MQ=255)
                                                                                                               tattGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGaa                                        <  2:4795120/150‑1 (MQ=255)
                                                                                                                attGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAg                                       >  1:2197372/1‑150 (MQ=255)
                                                                                                                 ttGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAgg                                      >  2:2879752/1‑150 (MQ=255)
                                                                                                                   gAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGat                                    >  1:2009492/1‑150 (MQ=255)
                                                                                                                    aGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGata                                   <  2:627669/150‑1 (MQ=255)
                                                                                                                      gggATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGAAAtt                                 >  1:82365/1‑150 (MQ=255)
                                                                                                                        gATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGc                               >  1:3011697/1‑150 (MQ=255)
                                                                                                                          ttCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCtt                             >  1:4948987/1‑150 (MQ=255)
                                                                                                                             gACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCTGACGGATATTGCTTTCg                          >  1:3620336/1‑150 (MQ=255)
                                                                                                                             gACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCg                          >  2:3137471/1‑150 (MQ=255)
                                                                                                                              aCCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGc                         <  2:1296762/150‑1 (MQ=255)
                                                                                                                                  cGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACg                     <  2:2347206/150‑1 (MQ=255)
                                                                                                                                   gTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACgg                    <  2:3313969/150‑1 (MQ=255)
                                                                                                                                        aGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCaaa               >  1:1898678/1‑150 (MQ=255)
                                                                                                                                           tGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAcc            <  1:4742778/150‑1 (MQ=255)
                                                                                                                                            gCCGGAGGAGGTTGTAACATCCTCCGGCTACCAGTTTAACCTCTAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGACGGATATTGCTTTCGCCACGGGCACAAccc           >  1:3134961/1‑150 (MQ=255)
                                                                                                                                             ccGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt          >  1:337342/1‑150 (MQ=255)
                                                                                                                                             ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt          >  1:1787899/1‑150 (MQ=255)
                                                                                                                                             ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt          <  1:1379327/150‑1 (MQ=255)
                                                                                                                                             ccGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt          <  1:4574706/150‑1 (MQ=255)
                                                                                                                                              cGGAGGATGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTg         >  2:2763364/1‑150 (MQ=255)
                                                                                                                                              cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCACAACCCTg         >  2:1731092/1‑150 (MQ=255)
                                                                                                                                              cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAccccg         <  1:4198949/150‑3 (MQ=255)
                                                                                                                                              cGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTg         <  1:4009089/150‑1 (MQ=255)
                                                                                                                                               ggaggaTGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGc        >  1:1662469/1‑150 (MQ=255)
                                                                                                                                               ggaggaTGTTACAACCTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGc        >  1:1732323/1‑150 (MQ=255)
                                                                                                                                               ggaggaGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTACCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGc        >  2:1135912/1‑150 (MQ=255)
                                                                                                                                                 aggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAc      <  2:2729708/150‑1 (MQ=255)
                                                                                                                                                 aggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAc      <  1:108169/150‑1 (MQ=255)
                                                                                                                                                   gaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCg    >  2:3505577/1‑150 (MQ=255)
                                                                                                                                                    aggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGa   <  1:3172307/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAA  >  NC_000913/1074745‑1075043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: