Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,798 T→A 7.0% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,7980TA 7.0% 508.2 / 6.2 215intergenic (+405/+310)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (92/94);  new base A (15/3);  total (115/100)
Fisher's exact test for biased strand distribution p-value = 6.27e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

GGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTT  >  NC_000913/2304650‑2304947
                                                                                                                                                    |                                                                                                                                                     
   tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                                                                                                                                   <  2:3548628/150‑1 (MQ=37)
     aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGc                                                                                                                                                 <  1:384141/150‑1 (MQ=12)
      ggCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGcc                                                                                                                                                >  1:1065326/1‑150 (MQ=37)
      ggCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGcc                                                                                                                                                >  2:1931530/1‑150 (MQ=37)
       gCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCATGATGCGCCGCTGGGGGGTCTTATCTGGCCTTCTCCGCTGTGATTTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                                                               <  1:267163/150‑1 (MQ=11)
       gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACTCCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                                                               <  1:1253865/150‑1 (MQ=37)
       gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                                                               >  1:176810/1‑150 (MQ=37)
           ttACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTTGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                                                                                                                                           <  1:1064195/150‑1 (MQ=255)
           ttACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                                                                                                                                           <  1:1064074/150‑1 (MQ=37)
            tccgccgcATCCGACATCCAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACTCCGCTGTGAAGTTCTGCACCCCGTAGGTTTGATTAGGCTGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                                                                                                                                          <  2:1084465/148‑1 (MQ=11)
            tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                                                                                                                                          <  2:3706987/150‑1 (MQ=37)
              cgccgcATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                                                                                                                                        <  1:45815/150‑1 (MQ=37)
              cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                                                                                                                                        <  1:4405985/150‑1 (MQ=21)
                 cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGATGGGGAGTTGACGACCACGTAGGTTGGATGAGGCGGTTACGCCGCATCCGACATCCTACGTCGGAGCCGGTTGCCTGAt                                                                                                                                     >  2:1801806/1‑150 (MQ=11)
                 cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAACGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt                                                                                                                                     >  1:934219/1‑150 (MQ=37)
                 cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCAACACCCATGCGGCGGGCGCCACCCCCGAGGTTGGCGGAGGCGGTTACGCAGCAACCGACATCCAACGTCCGAGCCGGTTGCCTGAt                                                                                                                                     >  2:4809089/1‑150 (MQ=11)
                 cgcATCCGACATCGAACGCCCGAGCCGTTTGCCTGATGCTAAGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt                                                                                                                                     <  2:32016/150‑1 (MQ=11)
                     tCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGa                                                                                                                                 <  2:472107/150‑1 (MQ=37)
                     tCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGa                                                                                                                                 <  2:441436/150‑1 (MQ=37)
                     tCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGa                                                                                                                                 >  1:3143193/1‑150 (MQ=37)
                      ccGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGAc                                                                                                                                >  2:739777/1‑150 (MQ=37)
                       cGACATCTAACGCCCAAGCTGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACg                                                                                                                               >  2:2258613/1‑150 (MQ=12)
                       cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACg                                                                                                                               >  1:3495138/1‑150 (MQ=37)
                             cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGTAGTGCTCCACCCCGTATGTTGGATAAGTCGGTTACGCCGCATCCGACATCAAACGTCCGAGCCGGATGCCTGATGCGACGCTGgcg                                                                                                                         >  2:2422641/1‑150 (MQ=11)
                              tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                        <  1:1429055/150‑1 (MQ=37)
                                aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                      <  1:1134748/150‑1 (MQ=37)
                                aCGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGGAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                      <  2:4845450/150‑1 (MQ=255)
                                aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                      <  2:5012270/150‑1 (MQ=37)
                                 cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                     >  2:432540/1‑150 (MQ=37)
                                   cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                   <  1:2049841/150‑1 (MQ=37)
                                   cccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTggt                                                                                                                                     <  1:3712928/132‑3 (MQ=37)
                                   cccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                   >  2:2430304/1‑150 (MQ=37)
                                    ccgaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTa                                                                                                                  <  1:2016252/147‑1 (MQ=37)
                                    ccAAGCCGGTTGCCAGATGCGACGCTGGCGCGTCTTATAAGGCCTAAACCCCAGTGAATTGCTCCACCCCCTAGGTTGGATAATGCGGTTACCCCGCAACCCACAACCAACAGACCAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTa                                                                                                                  >  1:982876/1‑150 (MQ=11)
                                     cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAATACACACCTCAGGAGTGCGCCACCCCGTAGGGTGGATAAGGCGGTAGCGCCGCAGCCGACAGCCATCGCCCCAGCCGGGGGCCTGATGCGCCGCTGGCGCGTCTTAt                                                                                                                 >  2:4392494/1‑150 (MQ=2)
                                      aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                >  1:3399544/1‑150 (MQ=21)
                                               gCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                       <  2:1483526/150‑1 (MQ=39)
                                                    aTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCt                                                                                                  <  1:1990131/150‑1 (MQ=39)
                                                       cGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                               >  2:4371075/1‑150 (MQ=39)
                                                        gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCAACCCCGTATGTAGGATAAGTAGGATACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTgaga                                                                                              >  1:5064874/1‑150 (MQ=11)
                                                         aCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                             <  1:432540/150‑1 (MQ=39)
                                                         aCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                             <  1:1693078/150‑1 (MQ=39)
                                                               gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                        >  1:2366289/1‑117 (MQ=39)
                                                                    tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTATTCAGGCCTACACACCTGTGAAGTGCTCCAccc                                                                                  >  2:3497533/1‑150 (MQ=11)
                                                                    tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCAGCCGCGTCTACTCAGGCCTACACCGCAGAGAAGTGCTCCAccc                                                                                  >  2:2870444/1‑150 (MQ=11)
                                                                     cTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAcccc                                                                                 <  1:67024/150‑1 (MQ=39)
                                                                       tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGt                                                                               >  1:3310337/1‑150 (MQ=39)
                                                                       tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                                                                                >  1:2509855/1‑101 (MQ=17)
                                                                         tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                             >  2:4521216/1‑150 (MQ=37)
                                                                           aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                              >  1:2554867/1‑115 (MQ=39)
                                                                           aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                              <  2:2554867/115‑1 (MQ=39)
                                                                              ccTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCgg                                                                        <  1:580420/150‑1 (MQ=255)
                                                                               cTACACCGCTGTGAAGTTCTCCACCCCGTAGGTATGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                                                       <  2:2596635/150‑1 (MQ=255)
                                                                                tACACCGCTGTGTAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCGCTGAGAAGTGCTCCACCCCGTATGGCGGAt                                                                      >  1:343052/1‑150 (MQ=11)
                                                                                tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGag                                                                      >  2:2819337/1‑149 (MQ=255)
                                                                                    ccGCTGTGAAGTGCGGCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                  <  2:887973/150‑1 (MQ=255)
                                                                                     cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACACCCCTGAGAAGTGCTCCACCCCGTAGGTCGGATAAGGc                                                                 >  2:1734468/1‑150 (MQ=255)
                                                                                           gAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCTCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAc                                                           <  1:4621676/150‑1 (MQ=255)
                                                                                           gAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTCAGAAGTGCACCACCCCGTCGGTCGGATCAGGCGGTTAc                                                           >  2:3322983/1‑150 (MQ=14)
                                                                                             aGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTAcgc                                                         >  2:310287/1‑150 (MQ=255)
                                                                                               tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                       <  2:3143193/150‑1 (MQ=255)
                                                                                               tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGCCGCGTCTTATCAGGCATACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATCGGGCGGTTAcgcag                                                       >  2:250161/1‑148 (MQ=255)
                                                                                                   ccACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGATCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCaac                                                   >  1:4369031/1‑148 (MQ=255)
                                                                                                   ccACCCCGTAGGTTCGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATc                                                   <  2:4318152/150‑1 (MQ=255)
                                                                                                    cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                  >  1:4119893/1‑150 (MQ=255)
                                                                                                    cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                  >  1:4771601/1‑150 (MQ=255)
                                                                                                     aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCg                                                 >  2:1105410/1‑150 (MQ=255)
                                                                                                     aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCCCTCAGACGTTCACCACCCCGTAGGTCGGATAATGCGGATACGCAGCAACCg                                                 >  1:3863991/1‑150 (MQ=14)
                                                                                                       gcgttAGGTTGGATATGGCGTTTACGCCGCATCCGACATCCACCGTCCGAGCGGGTTGCCTTTTGCGACGCTGGCGCGTATTATCAGGCCTACACGGCTGTGATGTGCTCCACCCCGTAGGTCGTTTATGGGGTTTACGCCGCATCCGAc                                               <  1:1542675/146‑1 (MQ=14)
                                                                                                       cccGTAGGTACGATAAGGCGGTCACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGAc                                               <  2:3838573/150‑1 (MQ=255)
                                                                                                        ccGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACa                                              <  2:593248/150‑1 (MQ=255)
                                                                                                           tAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                           >  1:2276051/1‑150 (MQ=255)
                                                                                                               ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                       >  2:538922/1‑150 (MQ=39)
                                                                                                               ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                       >  1:3522601/1‑150 (MQ=39)
                                                                                                               ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                       <  2:3495138/150‑1 (MQ=39)
                                                                                                                  gATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGccc                                    >  2:2067289/1‑150 (MQ=37)
                                                                                                                  gATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGccc                                    >  2:40244/1‑150 (MQ=37)
                                                                                                                   aTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTGACGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAGCTAACGcccc                                   >  2:1853887/1‑149 (MQ=255)
                                                                                                                   aTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                   >  1:856851/1‑150 (MQ=35)
                                                                                                                        gCGGTTACCCCGAATCCTACATCAAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGACTTAACAGCCCTACACCGCTGTCAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                              <  1:5011402/150‑1 (MQ=11)
                                                                                                                         cGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACGCCCAAGCCgg                             >  2:4281427/1‑150 (MQ=21)
                                                                                                                           gTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                           >  1:2091250/1‑150 (MQ=17)
                                                                                                                            ttACGCCGCATCCGACATCATACGACCGAGCCTGTTGCCTGATGCGACGCTGGAGGGTCTTATGAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                          <  2:1685791/150‑1 (MQ=11)
                                                                                                                              aCGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                                                            <  1:3025181/66‑1 (MQ=35)
                                                                                                                                 ccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                        >  1:1936481/1‑51 (MQ=35)
                                                                                                                                 ccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCGACCCCGCTGTGGAGTGCTCCCCCCCGTAGGTCGGCTAAGGCGGGGACGCCGCCGCCGCCCGCGCACGCCCCCGCCGGTGGccggg                     >  1:1450069/1‑147 (MQ=11)
                                                                                                                                 ccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCGTATCAGGCCTACACCGCTGTGTAGTGCTCCACCCCGGAGGGCGGATAAGGCGGTTACGCCGCATCCGACAGCTAACGCCCCAGCCGGTTGCCTgt                     >  1:4388766/1‑149 (MQ=11)
                                                                                                                                  cgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                    >  2:417290/1‑150 (MQ=32)
                                                                                                                                  agcATGCGACATACACGGTGCGAGCCGGTTGCGTGATGCGAATCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                    <  2:2630014/149‑1 (MQ=11)
                                                                                                                                      tCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTTTCAGGCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                >  1:613396/1‑150 (MQ=12)
                                                                                                                                      tCCGACATCCAACGTCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGCGACGGCGGTTACGCCGCAGCCGACCGCTAACGCCCCCGCCGGTTGCCTGAGGCGa                >  2:3722778/1‑150 (MQ=11)
                                                                                                                                      tCCGACATCCAACGTCCGAGCCGGTTGCCGGATGCGACGCAGGCGCGGCGTATCAGGCCGACACCGCTGGGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCCTCCGACAGCTAACGCCCCAGCCGGTTGCCTGATGCgc                >  1:1215984/1‑149 (MQ=11)
                                                                                                                                        cGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACg              >  2:3873054/1‑150 (MQ=17)
                                                                                                                                         gACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc             >  1:2918100/1‑150 (MQ=32)
                                                                                                                                         gACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc             >  1:2918051/1‑150 (MQ=32)
                                                                                                                                         gACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCCCTGTGAAGTGCGCCACCCCGTTGGTCGGATAAGGCCGTTACGCCGAAACCGACATCTAACGCCCACGCCGGTTGCCTGATGCGACGc             >  1:3255264/1‑150 (MQ=11)
                                                                                                                                               cAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTCGGATAAGGCGGATACGCCGCATCCGACATCTAACGCCCAAGCCGGATGCCTGATGCGACGCTGgcgc       >  2:1984633/1‑150 (MQ=12)
                                                                                                                                               cAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc       >  1:2766522/1‑150 (MQ=32)
                                                                                                                                               cAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc       >  2:786733/1‑150 (MQ=32)
                                                                                                                                                    tCCGAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCTTATCAGGCCTACTCCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt  <  2:4947901/150‑1 (MQ=11)
                                                                                                                                                    |                                                                                                                                                     
GGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTT  >  NC_000913/2304650‑2304947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: