Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 6.9% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 6.9% 516.6 / 18.9 191noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (90/83);  new base A (9/4);  total (103/88)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAG  >  NC_000913/1287001‑1287296
                                                                                                                                                    |                                                                                                                                                   
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                    >  2:2266130/1‑150 (MQ=255)
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                    >  1:4572753/1‑150 (MQ=255)
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                    <  1:1558569/150‑1 (MQ=255)
          gggCAGAATATTTGTTTGGGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCtt                                                                                                                                          <  2:4620021/150‑1 (MQ=255)
          gggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCTTCGGGTCGTGGCCTGCGCCAACGCACTCTCGCAGGCGCGCGAGGCGAACCtt                                                                                                                                          >  2:1838766/1‑150 (MQ=14)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                                                                                                                                        >  2:3125119/1‑150 (MQ=255)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                       >  2:4590364/1‑150 (MQ=255)
             cAGAATATTTGATTGCGGATTCGATTGAGATTTCAGGGACTTTAGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                       <  1:8811/150‑1 (MQ=255)
             cAGAATATTTGATTGCGGAGTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                       <  2:5465403/150‑1 (MQ=255)
                  tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACCCGCTCTCGCTGGCGCGCGAGTCGAACCATGGTCGAAg                                                                                                                                  >  1:5131294/1‑150 (MQ=14)
                  tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACACACGCGGGCGCACGAGTCGAACCCGGGTCGTAg                                                                                                                                  >  1:5131297/1‑150 (MQ=14)
                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAATCGAACCTTAGTCGAAGCt                                                                                                                                >  2:3496533/1‑150 (MQ=255)
                      tGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                              <  2:3338818/150‑1 (MQ=255)
                       gATTGCGGATTCGCTTGAGCGTTCAGGGACTTTTGAATGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtct                                                                                                                             <  1:964142/150‑1 (MQ=255)
                       gATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCACGCGGGCGCTCGAGTCGAAACATGGTCGAAGCGtca                                                                                                                             >  2:2393282/1‑149 (MQ=14)
                           gCGGGTTCGGTGGATCGTTCTGCGACGTTTGCAAGTGATGTGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                         <  1:2534929/150‑1 (MQ=11)
                             ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCt                                                                                                                       >  2:1818349/1‑150 (MQ=255)
                                   gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGAGCATCGCCCGTCGGGTCGATGCCTGCGGCAACGCGCTCTCGCTGGCGCGCGAGTCGCACCTTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                 >  2:2924173/1‑150 (MQ=14)
                                    cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCGTGGTCGTATCTTCTCAACCGTCACCGc                                                                                                                >  1:2209257/1‑150 (MQ=14)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTCACGCTGGCCCTCCCGTCGAACCGTGGTCGAATCATCACATCCATCCCCGCa                                                                                                               >  2:3245126/1‑150 (MQ=14)
                                       gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCTGCGGCAACGCTCACGCGCTGGCGCGCGTGTCGGAACATGGTCGAAGCTTATCAACCATCACCGCATg                                                                                                             >  1:1033595/1‑150 (MQ=14)
                                           gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACGAGTCGAACCTTGGTCGAAGCTTATCATCCTTACCCGCAACGGCa                                                                                                         >  2:3795060/1‑150 (MQ=14)
                                            ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCGTCCCCGCGTGGGccg                                                                                                        >  2:4426674/1‑148 (MQ=255)
                                            ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCATACATACCCGCAAGGGccg                                                                                                        >  1:4854533/1‑148 (MQ=14)
                                                gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAAAGCACTCTCGCTGGCGCTCCAGTCCAACCCTTGTAGAACCATCTCATCCATACCCCCAATGGCAGaaaa                                                                                                    >  2:1010084/1‑148 (MQ=11)
                                                 ggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCAAATGGGCAGCatat                                                                                                   >  2:452019/1‑150 (MQ=14)
                                                 ggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCACGCGGGCGCGCGAGTCGAACCGTGGTCGAAGCTTCTCATACATCCCCCCAGGGGCCGAataa                                                                                                   >  1:4887241/1‑149 (MQ=14)
                                                 ggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCACACGCAGGCGCGCGAGTGGAACCATGGTCGTAGCAGCTCATCCTTCCCCCCAGGGGCAGaaaga                                                                                                   >  1:5106505/1‑147 (MQ=14)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                               <  1:5214062/150‑1 (MQ=38)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                               <  1:3215221/150‑1 (MQ=38)
                                                      tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGTGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt                                                                                              >  2:4035493/1‑150 (MQ=255)
                                                      tttGAAAGTGAGGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCCACCCTCTCTCGCTGGCGCTCGAGGCGCACCGTGGTCGAAGCGTCTCATCCGTCCCCGCCAGGGCCGAATCTTTCAt                                                                                              >  2:1696490/1‑150 (MQ=11)
                                                         ctctgTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                           <  1:732722/146‑1 (MQ=255)
                                                          aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCACGCTGGCGCTCCACGCGAACCATGGTCGAAGCGTCTCATCCATCCCCGCAAGGGCCCCAGAATTAATTGCg                                                                                          >  2:1250701/1‑150 (MQ=11)
                                                           aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCGCACAAGCAGGCGCGCGAGTCGTAACGTGGTCGAAGCATCACAACAATACCCCCCGGGGCAGAAGAATTaaattggg                                                                                         >  1:2586279/1‑144 (MQ=2)
                                                            aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGa                                                                                        <  1:1470124/150‑1 (MQ=21)
                                                                  ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCACGCTGGCGCACGATTCGAACCTTGGACGAAGCATCTCATCCTTCCCCGCTTGGTCAGAATATTTAAATGCGGATTCGtt                                                                                  >  2:4583755/1‑150 (MQ=11)
                                                                        gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                            <  1:4494446/150‑1 (MQ=255)
                                                                        gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                            <  1:4494410/150‑1 (MQ=255)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                    <  1:3838575/150‑1 (MQ=255)
                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTAATTGCGGATTCGTTGGGGCTTTCAgggta                                                                   >  2:2625915/1‑148 (MQ=255)
                                                                                     cTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                               <  2:2987988/150‑1 (MQ=255)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                               >  2:3903716/1‑150 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGGCGAACCTTATCATACTTCCCCGCAGGGGCAGAATATTTAATGTCGGTTTCGTTGGGAAGTTAAGGGACTTTTg                                                              >  2:1995926/1‑150 (MQ=17)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                           <  2:4334788/150‑1 (MQ=255)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCCGGGAGGGTGGAAAGTgg                                                       >  2:5303601/1‑149 (MQ=14)
                                                                                               cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAATGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                     >  2:4382781/1‑150 (MQ=255)
                                                                                                   cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtgg                                                 <  1:2173768/150‑1 (MQ=255)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggt                                                <  1:1818349/150‑1 (MQ=255)
                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                              <  1:2965418/150‑1 (MQ=255)
                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                              <  2:493889/150‑1 (MQ=255)
                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                              <  1:2965286/150‑1 (MQ=255)
                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                              <  1:5184438/150‑1 (MQ=255)
                                                                                                           ttCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCAACCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCATGGACTTTTGAAAGTGATGGTGGGGGGGGaa                                         >  2:741462/1‑150 (MQ=17)
                                                                                                              agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGTAAGTGATGGGGGTGGGGGCAGGa                                      >  1:4292680/5‑150 (MQ=17)
                                                                                                                gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                    <  2:5131294/150‑1 (MQ=255)
                                                                                                                gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                    <  2:4007493/150‑1 (MQ=255)
                                                                                                                agcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGTTGGTGGTGGGGGAAGGAtt                                    >  2:2765184/3‑150 (MQ=255)
                                                                                                                agcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTGTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                    >  2:1927235/3‑150 (MQ=255)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCCCGCTGGCGCTCGAGACGAACCTTGGTCGAAGCTTCCCATCCTTCCCCGCATGGTCAGAATATTTAATTGCGGTTTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                   <  2:843063/150‑1 (MQ=14)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                <  1:4397897/103‑1 (MQ=255)
                                                                                                                    gtGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCCTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                <  2:3656698/149‑1 (MQ=255)
                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                   >  1:175661/1‑50 (MQ=38)
                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                   <  2:5148953/50‑1 (MQ=38)
                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                   <  2:175661/50‑1 (MQ=38)
                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                   <  2:4731660/50‑1 (MQ=38)
                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                  <  1:5442234/96‑1 (MQ=40)
                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                  >  2:2724600/1‑96 (MQ=18)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                           >  2:2967188/1‑150 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                           <  1:5155587/150‑1 (MQ=255)
                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCATCCATACCCCCAAGGGCAGAATATTTAAATTCGGATTAGGTGGGAAGTTCAGGGGCTTTTTTAAGTGATTGTGGTGGGGGTAGGATTAGAAccatag                          >  1:4961106/1‑146 (MQ=17)
                                                                                                                           cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGACGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGa                         >  2:4825689/1‑150 (MQ=255)
                                                                                                                             gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAACCATCTCATCCTTCCCCGAATGGGCAGAATATTTAATAGCGGATTCGTTGGGAAGATAAGGGACTTTTGAAAGTGATGGTGGTGGTGGAAGGATTCGAAACTACGAAg                       >  2:337632/1‑150 (MQ=17)
                                                                                                                               aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                          >  2:1433025/1‑65 (MQ=40)
                                                                                                                               aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                    <  1:1133212/103‑1 (MQ=255)
                                                                                                                               aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGGTGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTc                     >  2:5338371/1‑150 (MQ=255)
                                                                                                                               aaCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                    >  2:1133212/1‑103 (MQ=255)
                                                                                                                                 cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGATTAGAACCATCGAAGTCGa                   >  1:556819/1‑150 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                          <  1:2003836/78‑1 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                          >  2:2003836/1‑78 (MQ=21)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                          >  2:1506973/1‑78 (MQ=21)
                                                                                                                                    tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                              <  2:4056147/120‑1 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                              >  1:4056147/1‑120 (MQ=255)
                                                                                                                                      tctcGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg              <  1:2747982/150‑1 (MQ=255)
                                                                                                                                       ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAGTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                           <  1:4141958/104‑1 (MQ=255)
                                                                                                                                       ctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAGTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                           >  2:4141958/1‑104 (MQ=255)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGTAGTCGATGACGGc            >  2:2058760/1‑150 (MQ=255)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc            >  2:2058917/1‑150 (MQ=255)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGATTAGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc            >  1:5392806/1‑150 (MQ=255)
                                                                                                                                         cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa           <  1:3413811/150‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                >  2:2480601/1‑95 (MQ=255)
                                                                                                                                           cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                <  1:2480601/95‑1 (MQ=255)
                                                                                                                                             ggCGCTCGAGGCGAACCTTGGTCGACGCTTCTCAGCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt       <  1:5247749/150‑1 (MQ=255)
                                                                                                                                              gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                         >  2:3183815/1‑115 (MQ=255)
                                                                                                                                              gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                         <  1:3183815/115‑1 (MQ=255)
                                                                                                                                              gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                         <  1:3544185/115‑1 (MQ=255)
                                                                                                                                              gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                         >  2:3544185/1‑115 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc    <  1:4613238/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGATCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa   <  2:3148216/150‑1 (MQ=255)
                                                                                                                                                  tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGt                                                                                   >  1:1295609/1‑69 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAG  >  NC_000913/1287001‑1287296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: