Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,602,990 Δ1 bp 8.3% coding (1501/1536 nt) lsrA → autoinducer 2 import ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,602,9900T. 8.3% 124.6 / 7.3 48coding (1501/1536 nt)lsrAautoinducer 2 import ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (20/23);  new base . (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

AAAATGTGGCTGTGCTGCTTATCTCCTCCGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGT  >  NC_000913/1602844‑1603137
                                                                                                                                                  |                                                                                                                                                   
aaaaTGTGGCTGTGCTGCTTATCTCCTCCGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCTATATTAATGTCGAGACTATTATGCGCGTTGCCTTc                                                                                                                                                   >  1:610009/1‑149 (MQ=255)
     gggggtgtgCTGCTAAGCTCCTCCGACCTGGAAGAGAACGAACTGATGGCAGATCGGGTGTATGTGCTGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTAGGCGCGTTGCCTTCGGCGa                                                                                                                                              <  2:691402/144‑1 (MQ=255)
       ggCTGTGCTGCTTATCTCCTCCGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATa                                                                                                                                            <  1:371114/149‑1 (MQ=255)
       ggCTGTGCTGCTTATCTCCTCCGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATa                                                                                                                                            <  2:297075/149‑1 (MQ=255)
       ggCTGTGCTGCTTATCTCCTCCGACCTGGAAGAGATCGAACTGATGGCAGATCGTGGGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATa                                                                                                                                            <  2:96758/149‑1 (MQ=255)
                gctTATCTCCTCCGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGTATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTc                                                                                                                                   >  1:681148/1‑149 (MQ=255)
                     tcacctccGACCTGGAAGTGATCGAACTGATGGCAGATCGTGTGTATGTGATGGATCAGGGAGAAATTACCCACTCTGCACTGACCGTGAGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCaggag                                                                                                                              <  1:211903/146‑1 (MQ=255)
                         ctccGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGGGTCAGGAGGCGt                                                                                                                          >  2:281540/1‑149 (MQ=255)
                         ctccGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGt                                                                                                                          >  1:612041/1‑149 (MQ=255)
                         ctccGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCC‑TCGGCGATAGTCAGCGTCAGGAGGCGTc                                                                                                                         >  1:52318/1‑149 (MQ=255)
                         ctccGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCC‑TCGGCGATAGTCAGCGTCAGGAGGCGTc                                                                                                                         >  1:408425/1‑149 (MQ=255)
                          tccTACCTGGAAGAGATCGAACTGATGGCAGATCGAGTGTATGTGATGCATCAGGGGGATATTACCCACTCTGCACTGACCGAGCGCGTTATTATTGTCGAGAGTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTc                                                                                                                         <  1:399078/149‑1 (MQ=255)
                                   gAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTATGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAg                                                                                                                >  1:694148/1‑149 (MQ=255)
                                             aaCTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGa                                                                                                      >  2:281577/1‑149 (MQ=255)
                                             aaCTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGa                                                                                                      >  1:841558/1‑149 (MQ=255)
                                                tGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGaaca                                                                                                   >  2:841026/1‑149 (MQ=255)
                                                  aTGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGCCCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGaacaac                                                                                                 >  2:306364/1‑149 (MQ=255)
                                                  aTGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGCCCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGaacaac                                                                                                 >  1:63377/1‑149 (MQ=255)
                                                  aTGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGaacaac                                                                                                 <  2:295188/149‑1 (MQ=255)
                                                     gCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGAGTCATGCTGAAGTTTATTCAGAACAACCGt                                                                                              >  1:174692/1‑149 (MQ=255)
                                                      cAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTg                                                                                             <  2:1006942/149‑1 (MQ=255)
                                                        gATCTTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGaa                                                                                           <  1:390962/149‑1 (MQ=255)
                                                             tttgtATGTGATGCATCAGGGCGAAATTACACACTCTGCACTGAACGAGAGCGATATTAATGTCGAGACTATTATTCGAGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCAc                                                                                      <  2:610009/147‑1 (MQ=255)
                                                             tgtgtATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATTCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCAc                                                                                      <  1:560958/149‑1 (MQ=255)
                                                                   tgtgATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCAct                                                                                >  2:864728/1‑149 (MQ=255)
                                                                       aTGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTg                                                                                             >  2:597394/1‑132 (MQ=255)
                                                                       aTGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTg                                                                                             <  1:597394/132‑1 (MQ=255)
                                                                                ggCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGgtggtg                                                                   >  1:465995/1‑149 (MQ=255)
                                                                                ggCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCC‑TCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGtgc                                                                  <  2:52318/149‑1 (MQ=255)
                                                                                ggCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCC‑TCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGtgc                                                                  <  2:408425/149‑1 (MQ=255)
                                                                                         aCCCACTCTGCACTGACCGAGCGCGTTATTAATGTCGAGACTTTTATGCGCGTTGCCTTCGGCGATAGGCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGttt                                                          <  2:681148/149‑1 (MQ=255)
                                                                                         aCCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGAATATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGttt                                                          <  1:1029159/149‑1 (MQ=255)
                                                                                                ctGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTAc                                                   <  1:857731/149‑1 (MQ=255)
                                                                                                      tGACCGAGCGCGATATTAATGTCGAGACTATTCTGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTATATTCAGAACAACCTTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGtt                                             <  2:901634/149‑1 (MQ=255)
                                                                                                      tGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCCGGAGGCGTCATGCGGAAGGTTATGCAGAACAACCGTGAAATCACGGCACTGCTGTCGGTGGTGCTGCTGTTTGTAGTAGCCGGtt                                             >  2:703518/1‑149 (MQ=255)
                                                                                                      tGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGtt                                             <  2:271460/149‑1 (MQ=255)
                                                                                                      tGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGtt                                             >  1:85628/1‑149 (MQ=255)
                                                                                                          agAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCATCGGCGATAGTCGGCGTCAGGAGGCTTCATTCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCGGTTTGTATTACCCGGTTTtct                                         <  1:887551/148‑1 (MQ=255)
                                                                                                                  atatTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGcc                                 >  2:519757/1‑149 (MQ=255)
                                                                                                                           tCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGATTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGGTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTaa                        <  2:750745/149‑1 (MQ=255)
                                                                                                                           gcGAGACTATTATCCGCGTTTCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACATCCGTGAAATCACGGCCCTCCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTaa                        <  2:658275/148‑1 (MQ=255)
                                                                                                                             gagaCTATTATGCGCGTTGCCTTCGGCGATAGTCCGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAgt                      <  1:120562/149‑1 (MQ=255)
                                                                                                                             gagaCTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAgt                      <  1:841026/149‑1 (MQ=255)
                                                                                                                              agaCTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTTCCCGGTTTTCTCGACCGCCAGTATTTAAgtg                     >  2:823784/1‑149 (MQ=255)
                                                                                                                               gaCTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAgtgt                    <  2:85628/149‑1 (MQ=255)
                                                                                                                                         cgcgTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATAACGGCACTTCTGGCGCAGGTGCAGCTGTTTGTATTACACGGTTTTCTAGACCGCCAGTATTTAAGAGTGCAAACGCTg          >  2:17731/1‑149 (MQ=255)
                                                                                                                                           cgTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGCACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGAc        >  1:680534/1‑149 (MQ=255)
                                                                                                                                                 cTTCGGCGATAGTCAGCGTCAGGAGGCGGCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGt  <  1:425470/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAAATGTGGCTGTGCTGCTTATCTCCTCCGACCTGGAAGAGATCGAACTGATGGCAGATCGTGTGTATGTGATGCATCAGGGCGAAATTACCCACTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTCGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGT  >  NC_000913/1602844‑1603137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑