Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,669,275 C→A 13.0% L82I (CTC→ATC)  hcaE → 3‑phenylpropionate dioxygenase, large (alpha) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,669,2750CA13.0% 85.1 / 3.5 42L82I (CTC→ATC) hcaE3‑phenylpropionate dioxygenase, large (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base C (19/17);  new base A (3/3);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCAC  >  NC_000913/2669132‑2669419
                                                                                                                                               |                                                                                                                                                
aGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAAc                                                                                                                                             <  1:288090/149‑1 (MQ=255)
 gCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAAcc                                                                                                                                            <  2:913563/149‑1 (MQ=255)
       tcGTATATTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGAAAGATCCCAAACCACGGTGATTTCTTTAACACCTACATGGGAGAAGATGAGCTTGTCGTAGTTCGTAAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATgcc                                                                                                                                      <  2:648226/148‑1 (MQ=255)
                    cGTAGCTGGCTATTTATCGCACACGAAAGCCAGATCACAAAACCCGGTGATATCTTTAACAACTACATGGGAGAAGAAGCGGTTGTCGTAGTGCTTCTGAAAGACTGCAGGATCAAGGCGCAGATCAACCAATGCCGCCACCGGGCCAt                                                                                                                         >  2:124308/1‑149 (MQ=255)
                      ttGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGc                                                                                                                       >  1:661308/1‑149 (MQ=255)
                          tGGCAATTTCTCGCCCACGAAAGCCAGATCCGAAATCCCGGTCATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTCGTGCGTCACAAAGACGGAAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCgtgt                                                                                                                   <  1:152820/149‑1 (MQ=255)
                           ggCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGtgtg                                                                                                                  >  1:20726/1‑149 (MQ=255)
                            gCTATTTCTCCCCCACGACAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGa                                                                                                                 <  1:382501/149‑1 (MQ=255)
                                tttCTCGCCCACGAAAGCCAGATCCCAATACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGGGCGTCAGAAAGACTGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTa                                                                                                             <  1:621937/149‑1 (MQ=255)
                                tttCTCGCCCACGAAAGCCAGATCCCAAACCCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGAGCGTCAGAAAGACGGCAGCATCAAGGCGTTACTCAACCAATGCCGCCACCGGGCCATTCCTGTGAGTTa                                                                                                             >  2:174936/1‑149 (MQ=255)
                                  tctcGCCCAAGAAAG‑CAGATCCCAAAACCCGGTGAATTCTTTAACACCTACATGCGAGAAGAAACGCTAGTAGTAGTCCGACAGAAACACGGCATCATCAAGGCGTTTCACAACCAATGCCGCCACCGTGCCATGCGAGTGAGTTAtcc                                                                                                          >  2:23642/1‑147 (MQ=255)
                                    tcGCCCACGAAATCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGCTGCCGTAGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGGGTGAGTTATGCa                                                                                                         >  1:772763/1‑149 (MQ=255)
                                    tagCCCACGAAAGCCAGATCCAAAAACCCGGTGATTTCTTTAACACCTACAAGCGAGAAGAAGCGGTTGTCGTAGTGCGTCAGAAAGAAGCCAGCATCAAGGCGTTTCTCAACCAATCCCCGCACCGGGCCAACCGACTGAGATATGCa                                                                                                         >  2:557251/3‑149 (MQ=255)
                                         cACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTg                                                                                                    >  1:269080/1‑149 (MQ=255)
                                             aaaGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTGGTCGTAGTGCGTCTGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGc                                                                                                <  1:951288/149‑1 (MQ=255)
                                                         cAAAACCCGGTGATTTCTTTACCCCCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCACGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGcc                                                                                    >  2:913727/1‑149 (MQ=255)
                                                          aaaaCCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCACCCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCt                                                                                   >  2:81795/1‑149 (MQ=255)
                                                                  gTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGc                                                                           <  2:139977/149‑1 (MQ=255)
                                                                              acacCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGc                                                               >  1:378364/1‑149 (MQ=255)
                                                                                acCTACATGGTAGAAGATGCGGTTGTCGTAGTGCGTCAGAACGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTTAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTg                                                             <  1:534885/149‑1 (MQ=255)
                                                                                acCTACATGGGAGAAGATGAGGGTGGCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGAGTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTg                                                             <  1:363197/149‑1 (MQ=255)
                                                                                  cTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTATCACCCAATGCCGCCCCCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGGATCACTGCTggg                                                           >  2:870043/1‑148 (MQ=255)
                                                                                  cTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTATCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGt                                                           >  2:873987/1‑149 (MQ=255)
                                                                                   tACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGGATCAAGGCGTTTCTCAACCAATTCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTc                                                          <  2:995554/149‑1 (MQ=255)
                                                                                     cATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTATCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCtt                                                        <  1:873987/149‑1 (MQ=255)
                                                                                     cATGGGAGAAGATGCGGGTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTATCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCtt                                                        <  1:870043/149‑1 (MQ=255)
                                                                                               gaTGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGCTCTTATGGCATTaa                                              >  1:979850/1‑149 (MQ=255)
                                                                                                         tCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCAAGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGCTCTTATGGCAGTAAGGGCGAGTTg                                    >  2:28028/1‑149 (MQ=255)
                                                                                                           gTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCCTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTAACTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGAt                                  >  2:352434/1‑149 (MQ=255)
                                                                                                              gTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCCTGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGa                               >  1:1012629/1‑149 (MQ=255)
                                                                                                              gTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACTCTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACAGCGAGTTGATCGa                               <  2:269080/149‑1 (MQ=255)
                                                                                                               tGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGAt                              <  2:20726/149‑1 (MQ=255)
                                                                                                                 cGTCAGAAATACTGCAGCATCAAGGCGTTTATCATCGAATGCCGCCTTCGTGCCATGCGTGTGAGTTATGAAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACTGCTGTTCTTATGGCATTAACGGCGAGTTGATCGATGt                            <  1:23642/149‑1 (MQ=255)
                                                                                                                   tCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTAc                          <  2:661308/149‑1 (MQ=255)
                                                                                                                     aGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCTTGCGTGTGAGTTATGAAGATTGCGCAATCAATCGCGACTATAAATGCAAGTATCAAGGCTGGTCTAATGGCATTAACGGAGTGTTGATCGATGTACCg                        >  2:602580/1‑149 (MQ=255)
                                                                                                                     aGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCGTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCg                        >  2:846545/1‑149 (MQ=255)
                                                                                                                      gAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCGTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGc                       >  1:319960/1‑149 (MQ=255)
                                                                                                                         aGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTgg                    <  2:69522/149‑1 (MQ=255)
                                                                                                                           aCGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGAGCGATGTACCGCTGGaa                  <  1:57193/149‑1 (MQ=255)
                                                                                                                             ggCAGCATCAAGGCGTTTCTCAACCAAAGCCGCCACCGGTCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTAAGGCATTAACTCCGAGTTGATCGATGAACCGCTGCAAcc                >  1:84219/1‑149 (MQ=255)
                                                                                                                              gcagcaTCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCAAGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTTTGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCt               >  2:202334/1‑149 (MQ=255)
                                                                                                                                           gTTTCTCAACCCATGCCGCCCCCGGGCCAGGCGTGGGAGTTATGGAGATTGCGGCAAAACTCGCGCCTTTACCTGCCGGTATCACGGGGGGTCTTATGGAATTAACGGAGAGTTGAGCGATGTACCGCTGGAACCTCGCTCCTACCcac  <  2:608903/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAGAAAGACGGCAGCATCAAGGCGTTTCTCAACCAATGCCGCCACCGGGCCATGCGTGTGAGTTATGCAGATTGCGGCAACACTCGCGCCTTTACCTGCCCGTATCACGGCTGGTCTTATGGCATTAACGGCGAGTTGATCGATGTACCGCTGGAACCTCGCGCCTACCCAC  >  NC_000913/2669132‑2669419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑