Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,851,603 | C→T | 7.4% | S201S (AGC→AGT) | hypB → | GTP hydrolase involved in nickel liganding into hydrogenases |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,851,603 | 0 | C | T | 7.4% | 147.8 / 3.2 | 56 | S201S (AGC→AGT) | hypB | GTP hydrolase involved in nickel liganding into hydrogenases |
Reads supporting (aligned to +/- strand): ref base C (26/25); new base T (2/2); total (29/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 |
CGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAAC > NC_000913/2851459‑2851741 | cGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCg < 2:661579/149‑1 (MQ=255) gCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGAt > 1:924018/1‑149 (MQ=255) cacaCCAGCGATGCCGGTGACCACCGGTAAAGGCTGCCATCTTGACTCAAAGATGATTGCCGACGACGCACCGCGTCTGCCACTGGTCGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGAtc < 2:323216/149‑1 (MQ=255) acCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGCCGATATCGGTATTATGTTTATCGAACACGTTGGCAACCTCGTATCCCCGGCCAGCTTCGATCTCg > 1:568089/1‑149 (MQ=255) cAGCGCTTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGt < 1:397298/149‑1 (MQ=255) cAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGCCGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACGGGACGATAACGGTATTCTGTTTATCTAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCGCGGt > 2:786950/1‑149 (MQ=255) cAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGt > 1:111520/1‑149 (MQ=255) cAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGt > 1:115295/1‑149 (MQ=255) aGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTg > 2:242778/1‑149 (MQ=255) gCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTCTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCATCTTCGATATCCGTGa > 2:740740/1‑149 (MQ=255) cGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGaa < 2:598036/149‑1 (MQ=255) tCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAac < 1:176069/149‑1 (MQ=255) gTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAACCGTTGGCAACCTCGTATGCCCGGCCAGCTTCGGACTAGGTGAAAAACACaa > 2:578250/1‑149 (MQ=255) gTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACaa < 1:560773/149‑1 (MQ=255) ggTAAAGGCTCCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAAACTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGt > 2:328335/1‑149 (MQ=255) gTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTg > 1:24216/1‑149 (MQ=255) tAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGc > 2:595134/1‑149 (MQ=255) aaGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGACAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCtt > 2:17494/1‑149 (MQ=255) ggCTGCCATCTTTACGCACATATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTAGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTc < 2:707376/149‑1 (MQ=255) ggCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTc < 2:709029/149‑1 (MQ=255) tGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGt > 1:497817/1‑149 (MQ=255) cATCTTGACGCACAGATGATTGCCGACGCCGCACCTCGTCTGCCACTGGACGATAACGGTATTATGTTTATCGAAAACGTTGGCAACCTCGTATGCACGGCCGGCGTCGATCTCGCGGAAAAACCCGAAGTGGCGGTGCTTTCCGTTAc > 2:204245/1‑149 (MQ=255) tGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAg < 1:976907/149‑1 (MQ=255) tGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAg < 1:764283/149‑1 (MQ=255) cGCACAGAGGATAGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTg < 1:184112/149‑1 (MQ=255) cacaGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGaa < 1:28592/149‑1 (MQ=255) ttGCCTACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGAATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCa < 1:928170/149‑1 (MQ=255) gCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACt < 2:111520/149‑1 (MQ=255) gCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACt < 2:115295/149‑1 (MQ=255) ccGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCGAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAACCCACTg > 2:849824/1‑149 (MQ=255) ccGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGTTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTg > 1:591998/1‑149 (MQ=255) ccGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGTTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTg > 1:591996/1‑149 (MQ=255) cGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGa < 2:367920/149‑1 (MQ=255) cGACGCCGCACCCCGGCTGCCACTGGACGATTACGGTAATCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCTATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGAATAACCACTGa < 2:196661/149‑1 (MQ=255) ccgcACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAatat < 2:734477/149‑1 (MQ=255) cgTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCatat < 2:330260/149‑1 (MQ=255) gCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTTGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACAAAAAGTGGCGGTGCTTTCCGTTACCTAAGGTGAAGACAAAACACTGAAATATCCTCATATGttta > 1:274100/1‑148 (MQ=255) gCCACTGGACGATAACGGTATTCTCTTTATCGAAAACGATGGCAACCTCGTATGCACCGCCAGCTTCGATCTCGCTGAAAAACACAACGTGGCGGTGCTTTCCGTTTCCTAAGGTGAAGAAAAACCACTGAAATATCCGCATATGtttt > 2:891326/1‑148 (MQ=255) cTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGCCAAGCCACTGAAATCTCCGCATATGTTTgccgc > 2:768819/1‑149 (MQ=255) tAAAGGTATTCTGTTTAACGAAAACGTTGGCAACCTCTTATGCCCGGACATCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGc < 1:578250/149‑1 (MQ=255) ggTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGAt > 2:51285/1‑149 (MQ=255) gTATTCTGTTAATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAACCACAACGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGAtg < 2:8718/149‑1 (MQ=255) ttCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGGTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCGGCCGACTCGctgctgctg > 2:791140/1‑149 (MQ=255) cGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTGTCCGTTACCGAAGGGGAAGACCAACCACTGAAATATCCGCATATGTTTGCCGCCGCCGCGCTGATGCTGCTCACCAAAg > 1:865027/1‑149 (MQ=255) gAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAACCACTGAAAGATCCGCATATGTTTGCCGCCGCCGCGCTGATGCTGCTCAACAAAGt > 2:599358/1‑149 (MQ=255) aaaaCTTTGGGAACAGCGTATTACCGGCCAGCTTCGAGCTCGGTGAAAAACACAACGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGtt < 1:943943/149‑1 (MQ=255) aaaaCGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGtt > 2:423803/1‑149 (MQ=255) aaaaCGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGtt < 1:511517/149‑1 (MQ=255) aaaaCGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGtt > 1:476411/1‑149 (MQ=255) ggCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGtt < 2:24216/149‑1 (MQ=255) gCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTg > 1:1016242/1‑149 (MQ=255) ccgcGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCGTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGt < 2:924018/146‑1 (MQ=255) ccTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAATACACAAAGTGGAGGTGCTTTCCGTTACAGAAGGTGAAGACAAACCACTGCTATATCCGCATATGTTTGCCGCCGCGTCGCTGATGCTGCTCAACAAAGTTGACATGGTGCCGt > 2:87544/1‑149 (MQ=255) cGTATGCCCGGCCAGTTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAtc < 2:591998/149‑1 (MQ=255) cGTATGCCCGGCCAGTTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAtc < 2:591996/149‑1 (MQ=255) gCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAAc > 2:652470/1‑149 (MQ=255) | CGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAAC > NC_000913/2851459‑2851741 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |