Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,732,136 A→C 5.6% intergenic (+13/‑65) xylF → / → xylG D‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,732,1360AC 5.6% 264.4 / 3.9 109intergenic (+13/‑65)xylF/xylGD‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base A (46/54);  new base C (2/5);  total (48/61)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

Only 100 of 109 total aligned reads displayed.

CAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAA  >  NC_000913/3731990‑3732283
                                                                                                                                                  |                                                                                                                                                   
cAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAgc                                                                                                                                                   >  1:51745/1‑149 (MQ=255)
 aaaaGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAgcg                                                                                                                                                  >  1:439589/1‑149 (MQ=255)
            ccACACTGAATATTGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCggggg                                                                                                                                       >  2:599550/1‑149 (MQ=255)
             cacaCTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGc                                                                                                                                      <  1:388466/149‑1 (MQ=255)
             cacaCTGAATAATGGCCTGAAAGATGGCCCCTCCCTCCTCCTGACACCGATCGATGTGAATAAATACATCTTGAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGc                                                                                                                                      <  1:563240/149‑1 (MQ=255)
                acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGAAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTg                                                                                                                                   <  2:721011/149‑1 (MQ=255)
                 cTGAATAATGGCCTGACAGATCTCCCCACCCGCCACCTGACACCGATCGATGCGAATAAAAACAACCTCAAAGATCCGGAAAGTAAAGACGGAGTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGa                                                                                                                                  <  1:388668/149‑1 (MQ=255)
                  tGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTTAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                 <  2:685315/149‑4 (MQ=255)
                  tGAATAATGGCCTGAAAGATGACCCCTCCCGCCACCTGACCCCGATCGATGTGAATAAAAACAACATCAAAGATACGGTACTTAAAGACGGATTCCACAAACAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                 <  1:355898/149‑1 (MQ=255)
                    aataatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTCAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGTGTGAttt                                                                                                                               >  2:673946/1‑149 (MQ=255)
                      taatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGAGAATAAAAACAACATCAACGATACGGTACTTAAAGACGGATTCCTCAACGAGATCGAGCTGTAAGCGTCACGCCCCCGCTTCGCGCTGGGGCGtaacgctt                                                                                                                             <  2:766305/149‑8 (MQ=255)
                       aatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACACCATCAAAGATAGGGTAATTAAAGACGGATTCCACAAAGAGCGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctc                                                                                                                            <  2:349186/149‑1 (MQ=255)
                        atGGCCTGACAGATGCCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAACAACAACATCAAAGATACGCTAATTAAAGACGGAGTCCACAAAGCGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctct                                                                                                                           <  1:742848/149‑1 (MQ=255)
                         tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGTGGAGCGGGGGCGTGATTtctctc                                                                                                                          >  1:344904/1‑149 (MQ=255)
                                aaaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcg                                                                                                                   >  1:350878/1‑149 (MQ=255)
                                aaaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGAGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcg                                                                                                                   >  2:520577/1‑149 (MQ=255)
                                 atgATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATCCGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgc                                                                                                                  <  1:142807/147‑1 (MQ=255)
                                 aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTACTTAAAGACGGATTCCACAACGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgc                                                                                                                  <  1:277609/149‑1 (MQ=255)
                                  aGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                 <  1:919202/149‑1 (MQ=255)
                                    aTGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                               <  1:484642/149‑1 (MQ=255)
                                      gTCCCCTCCCGCCTCCTGACACCGTTCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGTCGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaa                                                                                                             <  2:377946/149‑1 (MQ=255)
                                       tCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCTAAGATACGGTAATTAAAGACGGATTCCACACAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaat                                                                                                            <  2:472990/149‑1 (MQ=255)
                                       tCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaat                                                                                                            <  1:599550/149‑1 (MQ=255)
                                        ccccTCCCGCCTCCTGCCACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCAAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatg                                                                                                           >  1:78066/1‑149 (MQ=255)
                                           cTCCCGCCTCCTGCCACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGCCGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTgaatgaat                                                                                                        <  1:872880/149‑1 (MQ=255)
                                           cTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTgaatgaat                                                                                                        >  2:973129/1‑149 (MQ=255)
                                            tCCCGCCTCCTCACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACCGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGGGATTTCTCTCCATGCCGCGTGAATGAAtt                                                                                                       >  1:29874/1‑149 (MQ=255)
                                                    cctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAACGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAgg                                                                                               <  1:42003/149‑1 (MQ=255)
                                                    cctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAgg                                                                                               <  2:898587/149‑1 (MQ=255)
                                                    cctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAgg                                                                                               <  1:45940/149‑1 (MQ=255)
                                                     ctGAAACCGAGCGATGTGAATAAAAACAACATCAAAGATACGGTAATACAAGACGGATTCCACAACGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGt                                                                                              <  2:1014081/149‑1 (MQ=255)
                                                      tGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTg                                                                                             <  2:136018/149‑1 (MQ=255)
                                                      tGACACCGAGCGATGTGAATAAAACCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCGTAGGTg                                                                                             <  2:532836/149‑1 (MQ=255)
                                                        acacCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGa                                                                                           >  1:541321/1‑149 (MQ=255)
                                                        acacCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGa                                                                                           >  1:858210/1‑149 (MQ=255)
                                                         cacCGATCGATGTGAATAAAAACAACATCAAAGATACGGGAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGac                                                                                          <  2:384643/149‑2 (MQ=255)
                                                          acCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCTTGAATGAATTGGCTTAGGTGGAGt                                                                                         >  2:956191/1‑149 (MQ=255)
                                                            cgagcgatGAGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCg                                                                                       <  1:879355/149‑1 (MQ=255)
                                                                  atgccaaTCAACACAACCTCAAAGATACCGTACTTAAAGCCGGATTCCACAAAGAGAGCGAGCTGTCAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGc                                                                                 <  1:166616/144‑1 (MQ=255)
                                                                  agctgAATAAAAACAACATCAAAGATACCGAAATTAAAGACGGATTCCCCAAAGAGAGCGAGCTGTACGCGATACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGc                                                                                 <  1:354843/146‑1 (MQ=255)
                                                                   tgtgAATAAAAACAACATCAAAGATACGGTAATTAAACACGGATTCCACAAAGAGAGCTAGCTGTATGCGTTAGGCCCCTGCGCTGAGGGGGGGCGTGTTTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATggc                                                                                <  1:328933/149‑3 (MQ=255)
                                                                    gtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCt                                                                               >  2:849508/1‑149 (MQ=255)
                                                                     ggAATAAAAACAACCTGATCGATACGGCAATAAACGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAAATGGCTTAGGTGGAGTCGTTATGCCtt                                                                              <  2:3803/148‑1 (MQ=255)
                                                                      gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGTTTCCACAAAGAGAGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGCGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTa                                                                             >  1:476689/1‑149 (MQ=255)
                                                                      gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTa                                                                             >  1:506686/1‑149 (MQ=255)
                                                                      gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTa                                                                             >  1:382692/1‑149 (MQ=255)
                                                                      gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCACAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGCATTGGCTTAGGTGGAGTCGTTATGCCTTa                                                                             >  1:215977/1‑149 (MQ=255)
                                                                      gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGAAAGCGTTACGCACAATCGCGTAGCCGGGGCGTGATTTCTCTCCCTGCCTCGTAAATTAATAGGATGAGCTGGAGTCTTTATGCATTa                                                                             >  1:467079/1‑149 (MQ=255)
                                                                      gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTAt                                                                            >  1:134954/1‑149 (MQ=255)
                                                                       aaTAAAAACATCATCAAAGATCGGGTAATTAAAGACGGATTCAACAAAGAGATCGAGCTGTAAGTGTTAAGCCCCAGCGCGGTGCGGGTGAGTGATTTCTCTCCTTGCCGCGTGATTGAATTGGATTAGGTGGAGTCGTTATGCCTTAt                                                                            <  2:950242/149‑1 (MQ=255)
                                                                       aaTAAAAACAACATCACAGATACGGTATTTAACCACGGATTCCACAAAGAGAGCGAGCTGTAAGCGGTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCAAGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTAt                                                                            <  2:772241/149‑1 (MQ=255)
                                                                       aaTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTAt                                                                            >  1:757899/1‑149 (MQ=255)
                                                                       aaTAAAAACAACATAAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGAAAGCGTTACGCCCCCGCGCGGTGCGGGGGCGTGATTTCTCTGCCTGCCGCGTGTATGAATTGGCTTAGGTGGAGTCGTTATGCATTAt                                                                            >  2:868498/1‑149 (MQ=255)
                                                                         tAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                          >  1:113138/1‑149 (MQ=255)
                                                                         tAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                          <  1:120382/149‑1 (MQ=255)
                                                                            aaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCGTATCTACt                                                                       >  1:881728/1‑149 (MQ=255)
                                                                             aacaacTTCAAAGAAACGGAAATTAAAGTCGGAATCCACAAAGAGTCCGCTCAGTAAGCGTTACGCCCCATCGCGGCGGGGGGGCGTGATTTCTCTCCATCCCGCGAGAATGAATTGGCTTAGGTGGAGGCGTTATGCCTTATCTACtt                                                                      <  1:903686/149‑1 (MQ=255)
                                                                             aacaacATCAAAGTTACGGTAATTAAAGACGGTTTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGAGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACtt                                                                      <  1:160100/149‑1 (MQ=255)
                                                                             aacaacATCAAAGATACGGTAATTAAAGACGGATTCCTCAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCTGGGGCGTGATTTCACTACATGCCGCGTGAATCAATTGGCTTAGGTGGAGGGGTTATGCCTTATCTAcct                                                                      >  1:699997/1‑147 (MQ=255)
                                                                               caacaTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGATCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGa                                                                    <  2:597477/149‑1 (MQ=255)
                                                                                    tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGATCTGTAAGCGTTACGCCCCAGCGCGGATCGGGGGCGTGATTTCTCTCCCTGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTCATATga                                                               >  2:64224/1‑149 (MQ=255)
                                                                                    tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATga                                                               <  1:829220/149‑1 (MQ=255)
                                                                                    tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATga                                                               <  1:869979/149‑1 (MQ=255)
                                                                                     cAAAGATACGGTCATTAAAGACGGATTCCACATAGATAGCGATCTGTAAGCGTTACGCCACAGCGCGGAGCGGGGGCGTGATTTCTCTCCATCCCGCGTGAATTTAATGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                              <  1:775745/149‑1 (MQ=255)
                                                                                     cAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                              <  2:433854/149‑1 (MQ=255)
                                                                                      aaaGATACGGTTATTAAAGACGGATTCCACAAAGAGAGAGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaag                                                             <  2:143940/149‑1 (MQ=255)
                                                                                       aaGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaaga                                                            >  2:371047/1‑149 (MQ=255)
                                                                                        aGATACGGAAATAAAAGCCGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGTTTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaagaa                                                           <  1:256325/149‑1 (MQ=255)
                                                                                               gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAcc                                                    >  1:805284/1‑149 (MQ=255)
                                                                                                  aTTAAAGACGTATTCCACATAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGAGTGATTTCTCTACATGACGCGTTATTGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCaaa                                                 <  1:73433/149‑1 (MQ=255)
                                                                                                  aTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGGAATGCCTTATCTACTTGAAATGAAGAACATTACCaaa                                                 <  1:972790/149‑1 (MQ=255)
                                                                                                     aaaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAAcc                                              >  1:196970/1‑149 (MQ=255)
                                                                                                       aGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGGGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCtt                                            >  2:485190/1‑149 (MQ=255)
                                                                                                         aCGGATTCCACCAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAATAACATTACCAAAACCATCg                                          <  2:912597/149‑1 (MQ=255)
                                                                                                          cGGATTCCACAATGAGCGCGAGCTGTACGCGTTACTCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCgg                                         <  1:695117/149‑1 (MQ=255)
                                                                                                          cGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCgg                                         <  2:123654/149‑1 (MQ=255)
                                                                                                          cGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGACCCAGCGCGGAGCGGGGGTGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCgg                                         <  1:673946/149‑1 (MQ=255)
                                                                                                           ggATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCATCGGc                                        >  2:845002/1‑149 (MQ=255)
                                                                                                              ttCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCCTGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAgt                                     >  1:450837/1‑149 (MQ=255)
                                                                                                                 cacaAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGCTTTCTCTCCATGCGGCGTGATTGAATTGGCTTAGGTGGCGGCGTTATGCCTTATCTACTTGAAATGAATAACATTACCAAAACCTTCGGCAGtgtg                                  >  2:814761/1‑149 (MQ=255)
                                                                                                                     aaGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGTGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAgg                              <  2:344904/149‑1 (MQ=255)
                                                                                                                      agagagCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTTTGCCTTATCTACTTGATATGAAGAACATTACCAAACCCTTCGGCAGTGTGAAggg                             >  1:739282/1‑148 (MQ=255)
                                                                                                                        tgagcgagcTGTATGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTTAATTAATTGGATTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTTTGAAGGCGa                           <  2:455336/148‑1 (MQ=255)
                                                                                                                          agcgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGAtt                         <  1:943099/149‑1 (MQ=255)
                                                                                                                            cgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTGATGTACTTGAAATGAAGAGCATTACCAAAACCTTCGGCAGTGTGAAGGCGCTTGa                       >  1:708976/1‑149 (MQ=255)
                                                                                                                                cTGTAAGCGTTACGCCCCAGCGCGGCGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAAc                   >  1:126083/1‑149 (MQ=255)
                                                                                                                                 tGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCCCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTAATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACg                  <  2:577110/149‑1 (MQ=255)
                                                                                                                                    aaGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGAGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCt               <  1:332288/149‑1 (MQ=255)
                                                                                                                                     aGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTg              >  1:788338/1‑149 (MQ=255)
                                                                                                                                     aGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAAATGGCTTAGGGGGAGTCGTTATGCCTTATGTACTTGAAATGATGAACATTACCAAAACCTTCGGCAGTGTGCAGGCGATTGATAACGTCTg              >  1:312662/1‑149 (MQ=255)
                                                                                                                                      gCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGc             <  2:858210/149‑1 (MQ=255)
                                                                                                                                         ttACGCCCAAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTAAGGTGTAGGCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAAAGTCTGCTTg          >  2:252964/1‑149 (MQ=255)
                                                                                                                                          caCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTTCTTGc         >  2:975177/2‑149 (MQ=255)
                                                                                                                                           aCGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGTAATGAAGAACATTACCGAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCg        >  1:707936/1‑149 (MQ=255)
                                                                                                                                           aCGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAAGGAATTGGCTTAGGGGGCGGCGTTATGCCTTATCTCCTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCg        <  1:668487/149‑1 (MQ=255)
                                                                                                                                            cGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCATAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTATGCATGCgg       >  1:53555/1‑149 (MQ=255)
                                                                                                                                             gCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTGTGCTTGCGGt      >  2:235474/1‑149 (MQ=255)
                                                                                                                                             gCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATCAAATGGCTTAGGTGCAGTCGTTATGCCATATCTACTTGCAATGAAGAACATTACCAAAACATTCGGCAGTGAGAAGGCGATTGATAACGTCTGCTTGCGGt      >  2:1894/1‑149 (MQ=255)
                                                                                                                                                 cAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGaa  <  2:419540/149‑1 (MQ=255)
                                                                                                                                                 cAGCGCGGAGCGGGGGCGTGATTTCTCTCAATGCAGCCTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACATTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCTGTTGaa  >  1:608101/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAA  >  NC_000913/3731990‑3732283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: