Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,817,948 G→T 5.1% E63D (GAG→GAT dinD → DNA damage‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,9480GT 5.1% 304.5 / 3.5 114E63D (GAG→GATdinDDNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base G (57/51);  new base T (4/2);  total (61/53)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 120 total aligned reads displayed.

TTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCC  >  NC_000913/3817800‑3818093
                                                                                                                                                    |                                                                                                                                                 
ttAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGCAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCgaggag                                                                                                                                                   >  2:433430/1‑149 (MQ=255)
    tGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATcc                                                                                                                                               >  2:355924/1‑149 (MQ=255)
     gCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATcca                                                                                                                                              >  1:542148/1‑148 (MQ=255)
      cAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTc                                                                                                                                             >  2:254463/1‑149 (MQ=255)
        aaCGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGa                                                                                                                                           >  2:532080/1‑149 (MQ=255)
          aggtgCATAACAATGCTCACGAAGACAACATGGGAAACTACTGGGTTATTCAGAGTATCGTCACTATATACGTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGTCCATTTCGAGGAGATCCTCGata                                                                                                                                         <  1:222355/145‑1 (MQ=255)
            gAGCAGAACAATGGTCAGCAAGACAAATTGTGATACTACTGTGTTATTCAGAGTATCGTCACTTTTTACCTGTATTAACGCGCGCCATAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATg                                                                                                                                       <  1:839032/149‑1 (MQ=255)
               cAGAACAATGGTCAGCAAGACAACTTGGGATACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTc                                                                                                                                    <  1:422172/149‑1 (MQ=255)
                   acacTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTCTCGTCACTTTATACCTGTATTAACGCGCCCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGAAGACCATATCGAGGATCTCCTCGAAATGGTCatca                                                                                                                                <  1:259138/149‑4 (MQ=255)
                   aCAATCGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCAGTGAGAACAGTGGTCACACAATTGAAGACCATATCGAGGATCACCTCGAAATGGTCatca                                                                                                                                <  1:262982/149‑4 (MQ=255)
                    cAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTAACACAAATCATGACCATTTCGAGGAGATCCTCGATATGGTCAAAAt                                                                                                                               >  1:205029/1‑149 (MQ=255)
                      aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGGCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTg                                                                                                                             <  2:665039/149‑1 (MQ=255)
                      aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTgg                                                                                                                            <  2:363983/149‑1 (MQ=255)
                             gCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaaa                                                                                                                      <  1:548035/149‑1 (MQ=255)
                              cAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAt                                                                                                                     >  2:966048/1‑149 (MQ=255)
                                 gACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCTATATGGTCAAAATTGGCTCAAATGcc                                                                                                                  >  2:826035/1‑149 (MQ=255)
                                   cAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaa                                                                                                                >  1:418607/1‑149 (MQ=255)
                                   cAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGAGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaa                                                                                                                >  1:414640/1‑149 (MQ=255)
                                    aaCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCGAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCaaaa                                                                                                               >  2:331268/1‑149 (MQ=255)
                                    aaCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACATAATACCTGTATTAACGCGAGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTAATGCCCATTTCGAGGAGATCCTCGATATGGTCAAAATTTGATCAAATGCCaaa                                                                                                               >  2:764467/1‑149 (MQ=255)
                                     aCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaaaa                                                                                                              <  2:73844/149‑1 (MQ=255)
                                      cTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAAGCCaaaat                                                                                                             >  1:211340/1‑148 (MQ=255)
                                       ttGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAaga                                                                                                            >  1:169689/1‑149 (MQ=255)
                                        tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAagag                                                                                                           <  2:553460/149‑1 (MQ=255)
                                        tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAagag                                                                                                           <  1:832328/149‑1 (MQ=255)
                                         gggAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGc                                                                                                          >  2:80110/1‑149 (MQ=255)
                                           gAAACTAATGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCTCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCTATGAGATCCTCGATATGGACAAAATTGTCTCACATGCCAAAAGAGCAt                                                                                                        >  2:581710/1‑149 (MQ=255)
                                              actactGGGTTATTCAGAGTATCTTCACTTTATACCTTTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaa                                                                                                     <  1:358740/149‑1 (MQ=255)
                                                tactGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAATCCTGTGAGATCAGTGGTCACACTATTGATGACCATTTCGAGGAGATCCTCGAGATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaaa                                                                                                   >  1:682711/1‑149 (MQ=255)
                                                   tGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGAc                                                                                                >  1:1009868/1‑149 (MQ=255)
                                                   tGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCCCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGAc                                                                                                >  2:872830/1‑149 (MQ=255)
                                                     ggTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACAt                                                                                              >  2:1025494/1‑149 (MQ=255)
                                                       ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTTATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                            <  2:27580/149‑1 (MQ=255)
                                                       ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                            >  2:614871/1‑149 (MQ=255)
                                                       ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                            >  1:27639/1‑149 (MQ=255)
                                                         aTTCAGAGTATCGTCACTTTATACCTGTATTAAAGCGCGCCAAAGTAGCCTGTGAGAACAGTGTTCACACTATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAATTGCCAAAAGAGCATTAAAAGACATCGTa                                                                                          <  2:363907/149‑1 (MQ=255)
                                                             agagTATCGTCACTTTATACCTGTATTAACGCGCGCCTAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGCTCATTAAAAGCCATCTTActct                                                                                      >  2:819619/1‑149 (MQ=255)
                                                              gagTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATTTGGTCAAAATTGTTTCAAATGACAAAAGAGCATTAAAAGACATCGTACtctc                                                                                     >  1:711423/1‑149 (MQ=255)
                                                               agTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTcc                                                                                    <  1:826574/149‑1 (MQ=255)
                                                                 tATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCAGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGTAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCg                                                                                  <  2:74995/149‑1 (MQ=255)
                                                                     gTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAt                                                                              <  2:404233/149‑1 (MQ=255)
                                                                         cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                          <  2:338348/149‑1 (MQ=255)
                                                                         cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                          <  2:179260/149‑1 (MQ=255)
                                                                          tttATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTg                                                                         >  2:340417/1‑149 (MQ=255)
                                                                             ataCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAACGACATCGTACTCTCACTCTATGCCTGTTa                                                                      >  2:921737/1‑149 (MQ=255)
                                                                               aCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTagc                                                                    >  1:866687/1‑147 (MQ=255)
                                                                               aCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                    >  1:876209/1‑149 (MQ=255)
                                                                                ccTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCt                                                                   <  2:237221/149‑1 (MQ=255)
                                                                                 cTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                  >  2:143205/1‑149 (MQ=255)
                                                                                  tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                               >  1:778400/1‑119 (MQ=255)
                                                                                  tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                               <  2:778400/119‑1 (MQ=255)
                                                                                  tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTgg                                                                 <  1:378868/149‑1 (MQ=255)
                                                                                   gTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                                <  1:262927/149‑1 (MQ=255)
                                                                                   gTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                                <  2:836692/149‑1 (MQ=255)
                                                                                    tATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgta                                                               <  1:818858/149‑1 (MQ=255)
                                                                                    tATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgta                                                               >  1:333059/1‑149 (MQ=255)
                                                                                     aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtag                                                              <  2:301730/149‑1 (MQ=255)
                                                                                      ttAACGCGCGCCAAAGAAGCCTGGGAGAACAGTTGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCAGgtagt                                                             <  1:545195/149‑1 (MQ=255)
                                                                                        aaCGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAc                                                           <  1:501182/149‑1 (MQ=255)
                                                                                          cgcgcgCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCAAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaa                                                         <  2:767971/149‑1 (MQ=255)
                                                                                           gcgcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaaa                                                        <  2:253827/149‑1 (MQ=255)
                                                                                             gcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCATCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAAc                                                      >  1:346681/1‑149 (MQ=255)
                                                                                                 cAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCg                                                  >  2:419260/1‑149 (MQ=255)
                                                                                                  aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGa                                                 <  1:30879/149‑1 (MQ=255)
                                                                                                  aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGCACAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATCCCTGTAACCTGGTAGTACAAAACGGCGa                                                 >  1:171444/1‑149 (MQ=255)
                                                                                                   aagaagCCTGTGAGAACAGTGGTCACAGAATTGATGACCATTTCTAGGAGATCCTCGATATGGTCAATATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAc                                                <  2:538533/149‑1 (MQ=255)
                                                                                                        tccTGTGAGAACAGTGGTCACTCAATTGATGTCTATTTTGAGTAGCTCGTCGATATGGTGATAATTGGCTCAAATGCCAAAAGTGTATTAAAAGACATCGTACTCTCGCGCTATGCCTGTTACCTGGTAGTACAAATCGGCGACCCTGc                                           <  2:500899/148‑1 (MQ=255)
                                                                                                            gtgAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGaaa                                       >  1:32177/1‑149 (MQ=255)
                                                                                                             ggagaACAGGGGTCACACAATTGAAGACCATTTCGATGAGATCCTAGTTATGGTCAAAATTGGCGCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAAc                                      <  1:738246/148‑1 (MQ=255)
                                                                                                              gagaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCTTTAAAAGACATCGTAGTCTCCCTCTATGCGTGTTTCCTGGTAGTACAAAACGGCGACCCTGCGAATcc                                     >  2:195166/1‑149 (MQ=255)
                                                                                                               agaACAGTGGTCACACAATTGATGACCATTTAGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAAGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCg                                    <  1:57722/149‑1 (MQ=255)
                                                                                                                taaCAGTGGTCACACAATTGATGACCATTTCGATGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCgg                                   <  2:205029/148‑1 (MQ=255)
                                                                                                                gaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCgg                                   <  1:501136/149‑1 (MQ=255)
                                                                                                                 aaCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAGAAAACGGCGACCCTGCGAAACCGGt                                  >  1:911864/1‑149 (MQ=255)
                                                                                                                  aCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTc                                 >  1:413533/1‑149 (MQ=255)
                                                                                                                        gTCACATTATTGATGACCTTTTCGAGGAGATGCTGGATATGGTCAATCTAGGCTCAAATGCCATAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGGTACCTGGTTGTCCAAAACGGCGACCCTGCGAAACCGGTCATTGCg                           <  1:486191/149‑1 (MQ=255)
                                                                                                                         tCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCgg                          >  1:1001516/1‑149 (MQ=255)
                                                                                                                             acaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATTACTAAAGAGAATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAGATGGTAGTACAAAACGGCGACCCTGCGCATCCGGTAATTGCggcagg                      >  1:487370/1‑149 (MQ=255)
                                                                                                                             acaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagg                      >  1:258997/1‑149 (MQ=255)
                                                                                                                              caATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcaggg                     <  2:545023/149‑1 (MQ=255)
                                                                                                                                aTTGATGACCATTTCGAGGAGATCCTCGATATGGCCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggca                   <  1:487470/149‑1 (MQ=255)
                                                                                                                                aTTGATGACCATTGCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggca                   <  2:614645/149‑1 (MQ=255)
                                                                                                                                    atgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGTGCAGACt               >  1:791021/1‑149 (MQ=255)
                                                                                                                                     tgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAATAGCATTAAAAGACATCGTACTATACCGCTATGCCTGTTACATGGTAGTACAAAACGGCTACCCTGCGATACCGGTCATTGCGGAAGGGCAGAcat              >  2:851597/1‑147 (MQ=255)
                                                                                                                                      gaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTa             >  2:48204/1‑149 (MQ=255)
                                                                                                                                      gaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGTAACCGGTCATTGCGGCAGGGCAGACGTa             >  1:989504/1‑149 (MQ=255)
                                                                                                                                       aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGCCCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt            >  1:315994/1‑149 (MQ=255)
                                                                                                                                       aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt            >  2:973407/1‑149 (MQ=255)
                                                                                                                                       aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt            >  1:945617/1‑149 (MQ=255)
                                                                                                                                        ccATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtt           <  1:387120/149‑1 (MQ=255)
                                                                                                                                         cATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAttt          >  2:639379/1‑149 (MQ=255)
                                                                                                                                         cATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAttt          >  1:20244/1‑149 (MQ=255)
                                                                                                                                          aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtttt         >  1:266301/1‑149 (MQ=255)
                                                                                                                                          aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtttt         <  2:43630/149‑1 (MQ=255)
                                                                                                                                           tttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAATGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTg        <  1:1019759/149‑1 (MQ=255)
                                                                                                                                            ttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGc       >  1:430970/1‑149 (MQ=255)
                                                                                                                                             tCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTTGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAttgttga      <  1:254463/149‑6 (MQ=255)
                                                                                                                                               gaggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCAATGCCTGTTAACTGGTCGTACAAAACGGCGACCCTGCGAAACCGGGCATTGCGGCAGGGCAGACTTATTTTGCTAt    <  2:900274/149‑1 (MQ=255)
                                                                                                                                                aggagATCCTCGATATGTTCAAATTTGGCTCAAATGACAAAAGAGGATTATAAGAAATCGTACTCTCCCGCTTTGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATc   <  1:764467/149‑1 (MQ=255)
                                                                                                                                                 ggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGATACCTGCTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATcc  <  1:355924/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCC  >  NC_000913/3817800‑3818093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: