Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,415,525 G→T 11.3% E417D (GAG→GAT)  aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,415,5250GT11.3% 97.8 / 3.6 48E417D (GAG→GAT) aidBDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (19/23);  new base T (4/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCG  >  NC_000913/4415379‑4415672
                                                                                                                                                  |                                                                                                                                                   
aaaGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGGGGCCGAAGCGATGGAGGGGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGAt                                                                                                                                                   <  1:476355/149‑1 (MQ=255)
     agCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGTCATTGGTTATTGCGACGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCgg                                                                                                                                              <  1:691041/149‑1 (MQ=255)
      gCCCTGTGGGCGAGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGt                                                                                                                                             <  2:741651/149‑1 (MQ=255)
         cTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaa                                                                                                                                          <  2:374988/149‑1 (MQ=255)
          tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAAc                                                                                                                                         <  1:100082/149‑1 (MQ=255)
             gggCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGt                                                                                                                                      >  1:947656/1‑149 (MQ=255)
             gggCGCGTTTATTTACGCCTGCCGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGCGCTGCATTGGTTATTGCGAGGAGAGTGAGCTGCCTCGGCTTTACCGGGAGATGCCGGTAAACAGt                                                                                                                                      >  2:35350/1‑149 (MQ=255)
                     ttatttaCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGa                                                                                                                              <  1:938109/149‑1 (MQ=255)
                         ttaCGCCTGCGGCAAAATTTGTGATCTGCCTATGAGGTATGCCGTTTGTGGCCGAAGCGATGGTGGTTCTGGGCGGCATTGGGTAGTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAATCAGTATTTGGGAAGGt                                                                                                                          >  2:645293/1‑149 (MQ=255)
                             gCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATTCCGGTAAACAGTAATTGGGAAGGTTCCg                                                                                                                      >  2:765554/1‑149 (MQ=255)
                              ccTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCgg                                                                                                                     >  1:53287/1‑149 (MQ=255)
                               cTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGc                                                                                                                    >  1:982597/1‑149 (MQ=255)
                                 gCGGCAAAATTTGTGATCTGCAAAAGATGTATGCCGTTTCTGGCCGAAGCGATGGAGGTGCTTGGCGGCATTGGTTATTGCGAGGAGAGTGAGCGGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCaa                                                                                                                  <  2:452647/149‑1 (MQ=255)
                                    gCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGTACGCAGCGATGGAGGTGCTGGGCGGCATAGGTTATTGCGAGGAGAGTGAGCTGCCGAGTCTTTACCGGGAGCTGCCGGTAATCAGTATGTGGGAAGGATCCTGCAatat                                                                                                               >  1:740496/1‑149 (MQ=255)
                                       aactttGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGCGGTGCTGGGCGGCTTTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtattat                                                                                                            <  2:573149/146‑1 (MQ=255)
                                       aaaTTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtattat                                                                                                            <  1:81201/149‑1 (MQ=255)
                                       aaaTTTGGGAGCTGCAAAAGCCGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtattat                                                                                                            <  1:559683/149‑1 (MQ=255)
                                        aagttGGGATCTGCCGAAGTGGTATGCGGGGTGTGGCCGATGGTATTGAGGTGCGGGGCGGCTTTGGGTTCTGGGAGGAGTGTGCGCTGCCGGGGCTTTACCGGGAGATGCCGGTATTCAGTATTTGGGTAGGTTCCGGCACTATTAtg                                                                                                           <  1:566875/146‑1 (MQ=255)
                                          tttGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgtg                                                                                                         >  1:232100/1‑149 (MQ=255)
                                              tgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGAGCTGGGCGGCTTTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGTTAAACAGAATTTGGGAAGGTTCCGGCAATATTATGTGCCTg                                                                                                     >  2:63310/1‑149 (MQ=255)
                                               gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTgg                                                                                                    >  2:85820/1‑149 (MQ=255)
                                                   tGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATATGGGAAGGTTCCGGCAATATTATGTGCCTGGAtgt                                                                                                <  1:160239/149‑1 (MQ=255)
                                                      aaaaGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGtt                                                                                             >  1:660064/1‑149 (MQ=255)
                                                        aaGAGGTATGCCGTTTGTGGCCGAAGCGAGGGAGGTGCGGGGCGGCATTGGTTATTGCGAGGAGAGGGAGCTGCCGCGGCGTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTgc                                                                                           <  2:404449/149‑1 (MQ=255)
                                                             gTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGtt                                                                                      >  2:363357/1‑149 (MQ=255)
                                                                  ccGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCaa                                                                                 <  1:793541/149‑1 (MQ=255)
                                                                   cGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGATCTCAAt                                                                                >  1:778903/1‑149 (MQ=255)
                                                                     tgtgtgGCCGAAGCGATGGCGGTGCGGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGATCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaa                                                                              <  2:649934/147‑1 (MQ=255)
                                                                       tgtgGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCTGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagc                                                                            <  1:765554/149‑1 (MQ=255)
                                                                             cGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                      <  1:546842/149‑1 (MQ=255)
                                                                               aaGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATTTGTTGCGCGTTCTCAATAAGCAAGCGGGc                                                                    >  1:140053/1‑149 (MQ=255)
                                                                                   gATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGTCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGtat                                                                >  1:838564/1‑149 (MQ=255)
                                                                                        aGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGAc                                                           <  1:46041/149‑1 (MQ=255)
                                                                                                ggcggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTc                                                   <  2:53287/149‑1 (MQ=255)
                                                                                                      aTTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCATTAAGCAAGCGGGCGTATACGACTTATTGTCGGAAga                                             >  1:858759/1‑148 (MQ=255)
                                                                                                      aTTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGc                                             >  2:29822/1‑149 (MQ=255)
                                                                                                               tGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGa                                    <  1:339600/149‑1 (MQ=255)
                                                                                                                gCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGaa                                   >  1:924692/1‑149 (MQ=255)
                                                                                                                gCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGaa                                   <  2:2122/149‑1 (MQ=255)
                                                                                                                    ggagAGGGAGCCGCCGCGGCTTTACCGGGAGATGCCGGGAAACAGTATTTGGGAATGTGCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGa                               <  2:712610/149‑1 (MQ=255)
                                                                                                                                   gcgGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCt                >  2:640133/1‑149 (MQ=255)
                                                                                                                                      tgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATCGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt             >  1:24638/3‑149 (MQ=255)
                                                                                                                                      gCTTTACCGGGAGATGCCGGTAAAGAGTATTTGGGAAGGTTCCTGCATTATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt             <  1:370817/149‑1 (MQ=255)
                                                                                                                                        tttACCGGGAGATGCCGGTATACAGTATTTGGGACGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTGTCAATAAGCATGCGGGGGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt           <  1:35350/149‑1 (MQ=255)
                                                                                                                                        tttACCGGCATCTCCCTGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTAAATAAGCAAGCGGGCGTAAAAGACATATTGTCGGAAGCATTTGTGGAAGTGAATGGGCACGATCGCTAtttt           >  2:709439/1‑149 (MQ=255)
                                                                                                                                        tttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt           >  1:760533/1‑149 (MQ=255)
                                                                                                                                        tttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt           >  1:644717/1‑149 (MQ=255)
                                                                                                                                           atcGGGATATGCCTGTAAACAGTCTTTGGGAATGTTCCGGCAATATTATGTTCCTGGATGTGTTGAGCGTTCTCAATAAGAAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt        <  2:947656/147‑1 (MQ=255)
                                                                                                                                           accttgagaTGCCGGTAAACAGTATTTGGGAAGTTTCCGGCAATATTATGTGCCTTGATGTGTTGCGCGTTCTCAATAAGCAAGCGTGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt        <  2:660064/144‑1 (MQ=255)
                                                                                                                                                 agaTGCCGGTAAACAGTATTTGGGAAGCTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcgcgcg  <  2:376829/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCG  >  NC_000913/4415379‑4415672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑