Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,448,424 T→A 5.8% intergenic (+189/‑23) ytfP → / → chpS GGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,448,4240TA 5.8% 278.9 / 3.2 99intergenic (+189/‑23)ytfP/chpSGGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (34/58);  new base A (2/4);  total (37/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATT  >  NC_000913/4448276‑4448572
                                                                                                                                                    |                                                                                                                                                    
gagaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACtt                                                                                                                                                      <  2:365740/149‑1 (MQ=255)
gagaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACtt                                                                                                                                                      >  1:187193/1‑149 (MQ=255)
 agaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACGAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACttt                                                                                                                                                     <  1:467844/149‑1 (MQ=255)
 agaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACttt                                                                                                                                                     <  2:388462/149‑1 (MQ=255)
 agaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACttt                                                                                                                                                     <  2:231191/149‑1 (MQ=255)
  gacgacTCGCATTCTTTTTTGTACTTCCCTCACCTTTTGCGATTCTATCCGAGCCGATTTCCATATCTATTAACGCATAAAAACCTCTGCTCGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTg                                                                                                                                                    <  2:568438/149‑1 (MQ=255)
   acgacTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGt                                                                                                                                                   >  2:1020636/1‑149 (MQ=255)
     gacTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGtat                                                                                                                                                 <  2:185879/149‑1 (MQ=255)
        tCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAAc                                                                                                                                              >  1:321518/1‑149 (MQ=255)
          gCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACtt                                                                                                                                            >  1:871383/1‑149 (MQ=255)
          gCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACtt                                                                                                                                            <  2:63284/149‑1 (MQ=255)
           cATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACAAAGTATAACtca                                                                                                                                           <  1:976639/149‑3 (MQ=255)
           cATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACAAAGTATAACtca                                                                                                                                           <  1:1020597/149‑3 (MQ=255)
            aTTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACTCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACAAAGTATAACtcac                                                                                                                                          <  1:218192/149‑4 (MQ=255)
            aTTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTACCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAACTGCGCAGTGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTaa                                                                                                                                          <  2:477354/149‑1 (MQ=255)
               cTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACTCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTATAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAagga                                                                                                                                       <  1:449306/149‑1 (MQ=255)
                tGTTTTGAAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTATCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAaggag                                                                                                                                      <  2:263363/149‑1 (MQ=255)
                   tttGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTg                                                                                                                                   >  1:205198/1‑149 (MQ=255)
                   tttGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTg                                                                                                                                   >  1:11768/1‑149 (MQ=255)
                    ttGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGc                                                                                                                                  <  1:97405/149‑1 (MQ=255)
                    ttGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGc                                                                                                                                  <  2:497317/149‑1 (MQ=255)
                        aaTTCCCTCACCTTTTGCTTTTCTCCCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACAGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGAt                                                                                                                              <  1:477812/149‑1 (MQ=255)
                            cccTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTACATATCTATTAACGCATAAAAAACTCTGCTTGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGt                                                                                                                          <  2:805323/149‑1 (MQ=255)
                            cccTCACCTTTTGCTGTGCTCTCCGAGCCGCGTTCCATATCTATTAACGCATAATAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGCAGCACCGTGAGGTATACTTTGTATAACTTAAGGAGGTGCAGATGCGt                                                                                                                          <  2:681645/149‑1 (MQ=255)
                             ccTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTa                                                                                                                         <  1:934293/149‑1 (MQ=255)
                               tCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTAtt                                                                                                                       <  2:321518/149‑1 (MQ=255)
                                cACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATACACAACTGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTTAGTTATACTCTGTATAACTTAAGGAGGTGCAGATGCGTATTa                                                                                                                      >  2:901687/1‑149 (MQ=255)
                                  ccTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAcc                                                                                                                    <  2:111514/149‑1 (MQ=255)
                                  ccTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAcc                                                                                                                    <  1:97956/149‑1 (MQ=255)
                                        gCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATaaaa                                                                                                              <  1:20342/149‑1 (MQ=255)
                                           tttCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGa                                                                                                           <  2:484222/149‑1 (MQ=255)
                                           tttCTCACCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGATATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGa                                                                                                           >  1:613809/1‑149 (MQ=255)
                                            ttCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGTTGCAGATGCGTATTACCATAAAAAGAt                                                                                                          >  1:996321/1‑149 (MQ=255)
                                              ctctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgg                                                                                                        <  1:60007/149‑1 (MQ=255)
                                                 tcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTTTACTTTGTATATCTTATGGAGGTGCAGATGCGTATTACCATAAAAAGATggggg                                                                                                     >  2:10290/1‑149 (MQ=255)
                                                 tcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACAATAAAAAGATgtggg                                                                                                     >  1:427543/1‑149 (MQ=255)
                                                   cGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCATGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGaa                                                                                                   >  1:821772/1‑149 (MQ=255)
                                                     aGCCGCGTTCCATATCTATTAACGCAAAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTAAAACTTAAGGACGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACa                                                                                                 <  2:721211/149‑1 (MQ=255)
                                                         gCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGc                                                                                             >  1:367587/1‑149 (MQ=255)
                                                                 tatCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATgg                                                                                     <  1:135061/149‑1 (MQ=255)
                                                                 tatCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATAAGTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATgg                                                                                     >  1:827638/1‑149 (MQ=255)
                                                                  atCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGt                                                                                    <  1:898290/149‑1 (MQ=255)
                                                                    cTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCa                                                                                  >  2:134410/1‑149 (MQ=255)
                                                                         aaCGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTccc                                                                             >  1:524336/1‑149 (MQ=255)
                                                                            gCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAat                                                                          >  1:683511/1‑149 (MQ=255)
                                                                               tAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATc                                                                       <  1:474101/149‑1 (MQ=255)
                                                                               tAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATc                                                                       <  2:378713/149‑1 (MQ=255)
                                                                                 aaaaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGt                                                                     >  1:959105/1‑149 (MQ=255)
                                                                                   aaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTAGGGTCATTCCCAATATCGTaa                                                                   <  1:210813/149‑1 (MQ=255)
                                                                                    accgctcctgGCATTCACAAATGCGCAGGGGTACAACGTTTCCTTTAGCACCGTGAGTTATACTTTGTATAACTTACGGAGGTGCAGATGCGTATTACCATAAATAGAGGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAAt                                                                  <  1:258891/142‑1 (MQ=255)
                                                                                      ctctGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATAACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGa                                                                <  1:378661/149‑1 (MQ=255)
                                                                                          gctgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTGAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGa                                                            >  1:213796/1‑149 (MQ=255)
                                                                                           ctgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaa                                                           <  2:1001403/149‑1 (MQ=255)
                                                                                            tgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaac                                                          <  1:484033/149‑1 (MQ=255)
                                                                                            tgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaac                                                          <  1:856838/149‑1 (MQ=255)
                                                                                                    acaAATGCGCAGGGGTAAAACGTTTCCTGTATCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCTGATGCGTATTACAATAAAAAGATGGGGGAACAGTGCATGTATGTTCATTCCAAATATCGTAATGAAAGAacttaactta                                                  >  2:251604/1‑149 (MQ=255)
                                                                                                    acaAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAacttaactta                                                  >  1:15903/1‑149 (MQ=255)
                                                                                                    acaAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAacttaactta                                                  >  1:914638/1‑149 (MQ=255)
                                                                                                    acaAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAacttaactta                                                  <  1:639539/149‑1 (MQ=255)
                                                                                                    acaAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACGTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATCGTCATTCCCAATATCGTAATGAAAGAacttaactta                                                  >  1:101119/1‑149 (MQ=255)
                                                                                                    acaAATGAGCAGGGGTAAAAAGTTTCCTGTAGCACCGTGAGTTATACTATGTATAACTTAAGGATGTGCAGATGCGTATTACCATAAAAAGATGGGCGAACAGTGCAGGTTTGGTCATTCCCAATATCTTAATGAAAGAacttaactta                                                  >  1:788945/1‑149 (MQ=255)
                                                                                                      aaaTGCGCAGGGGTAAAACGTTTCCTTTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACa                                                <  1:39444/149‑1 (MQ=255)
                                                                                                      aaaTGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACa                                                >  2:739438/1‑149 (MQ=255)
                                                                                                        aTGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGc                                              <  1:420290/149‑1 (MQ=255)
                                                                                                             cAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCgggg                                         <  1:38439/149‑1 (MQ=255)
                                                                                                             cAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCgggg                                         <  2:524336/149‑1 (MQ=255)
                                                                                                                gggTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCACAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCag                                      >  1:920497/1‑149 (MQ=255)
                                                                                                                 ggTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAACGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCaga                                     <  1:50040/149‑1 (MQ=255)
                                                                                                                  gTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGAGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTAAAGCCGGGCCagag                                    >  2:647036/1‑149 (MQ=255)
                                                                                                                    aaacCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCTGTTGCGTATTACCAGAAAATGATGGGGGAACAGTGCAGGTATGGTGATGCCCAATATCGTAATGAATGAACTTACCTTACAGCCGGGGCTGAGCg                                  >  2:151049/1‑149 (MQ=255)
                                                                                                                    aaaaCGTTTCCTGTAGCCCCGTGAGTTATACTTTGTATAACTGAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGCACTTAACTTACAGCCGGGGCAGAGCg                                  >  2:128999/1‑149 (MQ=255)
                                                                                                                     aaaCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTCCCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGt                                 <  2:683511/149‑1 (MQ=255)
                                                                                                                     aaaCGTTTCCTGAAGCACCGTGAGTTAAACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGt                                 <  2:6022/149‑1 (MQ=255)
                                                                                                                      aaCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTg                                <  2:874391/149‑1 (MQ=255)
                                                                                                                      aaCGGTTCCTGTAGCACCGTGAGTTATATTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATAGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGtt                                <  2:500207/149‑2 (MQ=255)
                                                                                                                       aCGTTTCCTGTAGCTCCGGGAGTTATACTTTGTTTATCTTAAGGTGGTGCAGTTGCGTTTTAGCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGttg                               <  2:300094/149‑3 (MQ=255)
                                                                                                                         gTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTggtg                             >  2:419497/1‑147 (MQ=255)
                                                                                                                             ccTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGgcgc                         <  1:426613/149‑1 (MQ=255)
                                                                                                                              cTGTAGCACCGTGAGTTATACTTTGTATAACTGAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCa                        >  1:806133/1‑149 (MQ=255)
                                                                                                                                 tatcagcGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACGATAAAAAGATGGGGGAACAGTGCATGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGt                     <  1:763028/143‑1 (MQ=255)
                                                                                                                                 tAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGt                     >  2:657576/1‑149 (MQ=255)
                                                                                                                                   gCACCGTTAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGa                   <  2:926844/149‑1 (MQ=255)
                                                                                                                                   gCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGa                   <  2:149925/149‑1 (MQ=255)
                                                                                                                                   ccACCGTGAGTTATACTATGTATAACTTAAGGAGGTGCAGATGCGTATAACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTTATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGa                   <  1:901687/148‑1 (MQ=255)
                                                                                                                                       cGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaa               <  1:193064/149‑1 (MQ=255)
                                                                                                                                         tGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaaca             <  2:513042/149‑1 (MQ=255)
                                                                                                                                            gTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATc          <  1:1020636/149‑1 (MQ=255)
                                                                                                                                              tataCTTTGTATAACTTAATGAGGTGCAGATGCGTATTACCATAAAAACATGGTGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGCGGCAGAGCGTTGAGGCGCAAGTGAGCAACAATCaa        <  1:1005861/149‑1 (MQ=255)
                                                                                                                                              tataCTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCaa        <  2:253883/149‑1 (MQ=255)
                                                                                                                                              tataATTTGTATAACTTAATGATTTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGGATGGTCATTCAAAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCaa        <  1:848935/149‑1 (MQ=255)
                                                                                                                                               ataCTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAAc       <  1:309002/149‑1 (MQ=255)
                                                                                                                                               ataCTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAAc       <  2:367587/149‑1 (MQ=255)
                                                                                                                                                taCTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACTATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACt      >  2:341929/1‑149 (MQ=255)
                                                                                                                                                  cTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAAAATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGa    >  2:471705/1‑149 (MQ=255)
                                                                                                                                                   tttGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGAt   <  1:134410/149‑1 (MQ=255)
                                                                                                                                                   tttGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGAt   >  2:624408/1‑149 (MQ=255)
                                                                                                                                                   tttGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGAt   <  2:187193/149‑1 (MQ=255)
                                                                                                                                                   tttGTATAACTTAAGGAGGAGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGCCAGAGCGTGGAGGCCCAAGTGAGCAACAATCACCTGa  >  2:161847/1‑148 (MQ=255)
                                                                                                                                                   tttGTATAACGTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTTAt   >  2:883401/1‑149 (MQ=255)
                                                                                                                                                    ttGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGAAGGGGGAACAGTGCAGGAATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGAtt  <  2:66603/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATT  >  NC_000913/4448276‑4448572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: