Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,958 G→T 7.3% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9580GT 7.3% 165.4 / 3.2 68intergenic (‑45/+28)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (36/27);  new base T (2/3);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

CCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGA  >  NC_000913/4470811‑4471104
                                                                                                                                                   |                                                                                                                                                  
ccGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAggg                                                                                                                                                   >  1:642923/1‑149 (MQ=255)
ccGGGATCTGACCGGAGGTGATGATAATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTAGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAggg                                                                                                                                                   <  2:474030/149‑1 (MQ=255)
 cGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgggg                                                                                                                                                  >  1:541069/1‑148 (MQ=255)
 cGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATCATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgggg                                                                                                                                                  >  1:422091/1‑148 (MQ=255)
   ggATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGc                                                                                                                                                >  1:349951/1‑149 (MQ=255)
     aTCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCa                                                                                                                                              <  1:387500/149‑1 (MQ=255)
      tCTGACCGGAGGTGATGAGCATATTGCCCAGATCAACGCCCTGTACGTAACGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAg                                                                                                                                             <  2:137245/149‑1 (MQ=255)
          aCCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGAAAGCAGCCGGGGCATTTTCCGTCGCGCGAGGTTTGCACATGATTTCTCCATTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtt                                                                                                                                         >  2:692479/1‑149 (MQ=255)
           ccGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACttt                                                                                                                                        <  1:611884/149‑1 (MQ=255)
            cGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGGTTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtttt                                                                                                                                       >  2:394821/1‑149 (MQ=255)
            cGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtttt                                                                                                                                       <  1:1022785/149‑1 (MQ=255)
               aGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttatt                                                                                                                                    >  1:39810/1‑149 (MQ=255)
                ggTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttatta                                                                                                                                   <  2:1002390/149‑1 (MQ=255)
                 gTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAc                                                                                                                                  >  2:44103/1‑149 (MQ=255)
                   gatgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCa                                                                                                                                >  2:357900/1‑149 (MQ=255)
                     tgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAAc                                                                                                                              <  2:642923/149‑1 (MQ=255)
                      gatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAAcc                                                                                                                             >  2:488255/1‑149 (MQ=255)
                       atCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGATTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCg                                                                                                                            <  1:960934/149‑1 (MQ=255)
                       atCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTACGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCg                                                                                                                            <  2:522259/149‑1 (MQ=255)
                         cATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCa                                                                                                                          >  2:606659/1‑149 (MQ=255)
                             ttGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaatta                                                                                                                      >  1:147086/1‑149 (MQ=255)
                                cccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaatt                                                                                                                   <  2:390448/149‑1 (MQ=255)
                                 ccAGCTCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGGGCATTTTCCCTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCTGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTg                                                                                                                  <  2:838176/149‑1 (MQ=255)
                                   aGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGc                                                                                                                >  2:362449/1‑149 (MQ=255)
                                    gATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGcc                                                                                                               >  1:588424/1‑149 (MQ=255)
                                                 tACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAACAACCGCAATAAATTGGCGAGCACGACATTAt                                                                                                  >  1:539092/1‑149 (MQ=255)
                                                   cGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATgg                                                                                                >  2:176561/1‑149 (MQ=255)
                                                    gTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATggg                                                                                               >  2:957578/1‑149 (MQ=255)
                                                       aGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaa                                                                                            >  2:610007/1‑149 (MQ=255)
                                                        ggACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaaa                                                                                           >  1:954804/1‑149 (MQ=255)
                                                            cGATAGCTGCCGGTGCATTTTCCGTCGCGATAGATTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActct                                                                                       <  2:270322/149‑1 (MQ=255)
                                                                  cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca                                                                                 <  2:147086/149‑1 (MQ=255)
                                                                    gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTAATACAGCGGTAAAAAGTCTGGCTCTTTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGTAAAACTCTTTTTcaca                                                                               <  2:513219/149‑1 (MQ=255)
                                                                     ccGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAg                                                                              >  1:162312/1‑149 (MQ=255)
                                                                            aTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc                                                                       >  2:312955/1‑149 (MQ=255)
                                                                            aTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATAATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc                                                                       <  1:522934/149‑1 (MQ=255)
                                                                            aTTTTCCGTCGAGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATTGGGAGCCAGACATTTATTAACAACCGCAATTAATTGGCCTGCACCACATTATGGGAAATCTCTTTTTCAGAGTATTTgt                                                                       >  2:114575/1‑148 (MQ=255)
                                                                             ttttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCa                                                                      >  2:905322/1‑149 (MQ=255)
                                                                                   gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAg                                                                >  1:368050/1‑149 (MQ=255)
                                                                                   gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAg                                                                >  2:284977/1‑149 (MQ=255)
                                                                                    tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGCCTTTTATTACCAACCGCAATTAATTGGACAGCACCACATTTTGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgc                                                               >  2:599332/1‑149 (MQ=255)
                                                                                    tCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgc                                                               >  1:1022962/1‑149 (MQ=255)
                                                                                           aGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTAGAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGAACCACATTATGGGAAATCTATTTTTCACAGTATTTGCATTTGAGCGCGattt                                                        >  1:589824/1‑147 (MQ=255)
                                                                                                tGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCAATATCATTg                                                   <  2:39810/149‑1 (MQ=255)
                                                                                                  cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc                                                 >  1:444695/1‑149 (MQ=255)
                                                                                                   tCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg                                                >  1:515792/1‑149 (MQ=255)
                                                                                                    cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcgc                                               <  2:162312/149‑1 (MQ=255)
                                                                                                      tGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGt                                             <  1:905322/149‑1 (MQ=255)
                                                                                                          ttCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAAGTCGTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGGGTTTTc                                         >  2:570678/1‑149 (MQ=255)
                                                                                                          ttCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc                                         <  1:284977/149‑1 (MQ=255)
                                                                                                          ttCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTc                                         <  1:294469/149‑1 (MQ=255)
                                                                                                           tctcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg                                        <  2:589547/149‑1 (MQ=255)
                                                                                                            ctcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGTGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGc                                       <  1:592202/149‑1 (MQ=255)
                                                                                                             tcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                      >  2:384790/1‑149 (MQ=255)
                                                                                                             tacTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                      <  1:208699/147‑1 (MQ=255)
                                                                                                               cTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACg                                    <  1:44103/149‑1 (MQ=255)
                                                                                                                    ttaCAGCGGTAAAAAGTCTGGCTCAGTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTTTGGGAAAACTCTATTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaaa                               >  2:529230/1‑149 (MQ=255)
                                                                                                                      aCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGc                             >  2:430942/1‑149 (MQ=255)
                                                                                                                       cAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCt                            >  1:540708/1‑149 (MQ=255)
                                                                                                                       cAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCt                            >  1:693065/1‑149 (MQ=255)
                                                                                                                         gCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg                          >  2:453377/1‑149 (MQ=255)
                                                                                                                          cGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCCGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGa                         <  1:692479/149‑1 (MQ=255)
                                                                                                                                 aaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc                  >  2:175569/1‑149 (MQ=255)
                                                                                                                                  aGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGCTATCATTGGCGCGTTTTCGCCCGGCAAAGCTGGATGAAACCg                 >  1:448361/1‑149 (MQ=255)
                                                                                                                                     cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt              <  2:349951/149‑1 (MQ=255)
                                                                                                                                      tGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCTCATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc             <  2:532217/149‑1 (MQ=255)
                                                                                                                                        gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATTGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg           <  2:357841/149‑1 (MQ=255)
                                                                                                                                        gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTATTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg           <  2:634814/149‑1 (MQ=255)
                                                                                                                                         cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc          <  2:954804/149‑1 (MQ=255)
                                                                                                                                             ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg      <  1:357900/149‑1 (MQ=255)
                                                                                                                                                 aGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa  >  1:491800/1‑149 (MQ=255)
                                                                                                                                                 aGGGAGCCAGACTTTTATAACCAACCGCAATTAAATCGCCAGCACAACATTATGGGAAAACTCTTTTTCACAGAATTTGCATTTGAGAGCGATATCAATGGCCCGTTTTCGCAAAGCAAAGCTGGATGATACAGGTACGCCATGGCTGa  >  1:284451/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGA  >  NC_000913/4470811‑4471104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: