Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,743 C→T 38.1% M1M (ATG→ATA) † yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,7430CT38.1% 35.2 / 47.8 48M1M (ATG→ATA) †yjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base C (14/15);  new base T (10/8);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

CCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGCTGAC  >  NC_000913/4633598‑4633882
                                                                                                                                                 |                                                                                                                                           
ccTCCCTGCCCACCGGCTTTTCCGGTCCACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa                                                                                                                                          <  2:844922/149‑1 (MQ=255)
  tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATCGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                                                                                                                        <  1:107278/149‑1 (MQ=255)
          aaaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTa                                                                                                                                >  2:830031/1‑149 (MQ=255)
           aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTAc                                                                                                                               <  2:512922/149‑1 (MQ=255)
             gagCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCGTGCTGAATGGCCTGCATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGGGTATAATCAGCATTACTc                                                                                                                             >  1:216635/3‑149 (MQ=255)
                       ggTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCTAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa                                                                                                                   <  1:300343/149‑1 (MQ=255)
                       ggTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa                                                                                                                   >  1:528806/1‑149 (MQ=255)
                              cGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTAca                                                                                                            <  1:346965/149‑1 (MQ=255)
                              cGCTCTCGACGGCAACGGATGCAATAAGGCAGGATCCTTCGCCGAAGATCTCGTGAAAAGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGCCAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAca                                                                                                            >  1:637735/1‑149 (MQ=255)
                                        ggCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCCGCATTACTCAGAAAATTAACGTTACAGCAGTATACg                                                                                                  >  2:870719/1‑149 (MQ=255)
                                         gCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACgg                                                                                                 >  1:382042/1‑149 (MQ=255)
                                          cAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGtt                                                                                                               <  2:959324/134‑1 (MQ=255)
                                          cAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGtt                                                                                                               >  1:959324/1‑134 (MQ=255)
                                                aTGCAATATTGAAGGAAGATTCGCCGTAGATATGGTGAATTGCCTGATGATTGGGCTGAATTTTAGCGTGATTGGTGGTCGCAGATACAACTTGGTGAATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGaaaaaaa                                                                                          <  2:944157/149‑1 (MQ=255)
                                                 tGCAATATGGCAGGATCCTTCTCCGAAGATCTCGAGAAATGCCTGCAGAATGGCCTGAATTTTAGCGTTATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAg                                                                                         <  1:380515/149‑1 (MQ=255)
                                                  gCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGc                                                                                        <  2:616641/149‑1 (MQ=255)
                                                    aaTATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCAt                                                                                      >  1:517925/1‑149 (MQ=255)
                                                      tatGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGt                                                                                    >  1:73781/1‑149 (MQ=255)
                                                               aTCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATTGCCTGAATTTTAGCGGGTTTGGTGGGCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCATTATACGGAAAAAAAGCATGTTACAGGtat                                                                           <  2:456848/149‑1 (MQ=255)
                                                                 ccTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACTGAAAAAAAGCATGTTACAGGTATAc                                                                         <  1:800674/149‑1 (MQ=255)
                                                                  cTTCGCCGAAGATCTCGTGAAATTCCTGCAGAATGGCCTGAATAATAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGTTATAcc                                                                        >  1:682706/1‑149 (MQ=255)
                                                                   ttCGCCGAAGATCTCGTGAAATGCCTGCAGAATGTCCTGAAATTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATCTTACAGGAATAcat                                                                       >  1:1005338/1‑147 (MQ=255)
                                                                    tCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATAACTa                                                                      >  1:924622/1‑149 (MQ=255)
                                                                     cGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGGTGTAGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAg                                                                     <  2:246925/149‑1 (MQ=255)
                                                                        cGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTcc                                                                  >  2:575446/1‑149 (MQ=255)
                                                                           aGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGcc                                                               <  2:447466/149‑1 (MQ=255)
                                                                             aTCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCAc                                                             >  1:3018/1‑149 (MQ=255)
                                                                              tctcGTGAAATGCCTGCAGAATGGCATGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAGAAAAAGCATGTTACAGGTATACCTAGTCCGCCACg                                                            <  1:860212/149‑1 (MQ=255)
                                                                                tcGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGt                                                          >  1:794410/1‑149 (MQ=255)
                                                                                tcGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGt                                                          <  1:455040/149‑1 (MQ=255)
                                                                                  gTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGa                                                        <  1:878237/149‑1 (MQ=255)
                                                                                  gTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGGTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGa                                                        <  1:656158/149‑1 (MQ=255)
                                                                                    gAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTAGCACATAAAACTTTGTTCATATTCAGCATTACTGAGAAACTTAACTGTACAGGAGTATACGGATAAAAAGCATGTTACAGGTATACCTAGTACGCCTCGGTGaaa                                                      <  2:254931/149‑1 (MQ=255)
                                                                                           cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTg                                               >  1:141348/1‑149 (MQ=255)
                                                                                           cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTg                                               >  1:236988/1‑149 (MQ=255)
                                                                                           cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAATAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGCTATACCTAGTCCGACACGGTGAAAAGCactt                                               >  1:586356/1‑146 (MQ=255)
                                                                                               aGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAAc                                           <  1:830031/149‑1 (MQ=255)
                                                                                                gAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTACCGTTACAGCAGTATACGGAAACACAGCATGTTACAGCTATACCTAGTCCGCCACGGGGAACCGCAGTGGACCg                                          >  1:334506/1‑149 (MQ=255)
                                                                                                        tGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCTTTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGCTATACCTAGTCCGACACTGAGAAACGCAGTGGACCGCCGAGCGa                                  >  2:260110/1‑149 (MQ=255)
                                                                                                         taaTTTTCGCTGGATTGTTGGGTTAACAGAGAAATTGGTGTATCATGAGCTTATCTCAGAAAATTAACGTTACAGAAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCAGCCACGTTGAAACGCAGTGTAACGCCGAGCGAc                                 <  2:965112/148‑1 (MQ=255)
                                                                                                               tAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTc                           >  1:564756/1‑149 (MQ=255)
                                                                                                               tAGCGGGATAGGTGGTCGCACAGAAAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGGTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTc                           <  1:414317/149‑1 (MQ=255)
                                                                                                                 gCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCAATACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGATACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAg                         <  2:528806/149‑1 (MQ=255)
                                                                                                                   gggATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATCCAggg                       <  1:870719/149‑1 (MQ=255)
                                                                                                                       ttGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAg                   >  2:956506/1‑149 (MQ=255)
                                                                                                                         ggtggtCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTc                 <  1:359482/149‑1 (MQ=255)
                                                                                                                                    aGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGATACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATAACTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGc      >  2:207487/1‑149 (MQ=255)
                                                                                                                                        aaCTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTCAAACGCAGTGTAACGCAGAGCGACGTATTCAGCGCCAGTCTGACAGCCCGCTGAc  >  1:11482/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
CCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGCTGAC  >  NC_000913/4633598‑4633882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: