Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,633,743 | C→T | 38.1% | M1M (ATG→ATA) † | yjjX ← | non‑canonical purine NTP phosphatase, ITPase/XTPase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,633,743 | 0 | C | T | 38.1% | 35.2 / 47.8 | 48 | M1M (ATG→ATA) † | yjjX | non‑canonical purine NTP phosphatase, ITPase/XTPase |
Reads supporting (aligned to +/- strand): ref base C (14/15); new base T (10/8); total (24/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.66e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 |
CCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGCTGAC > NC_000913/4633598‑4633882 | ccTCCCTGCCCACCGGCTTTTCCGGTCCACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa < 2:844922/149‑1 (MQ=255) tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATCGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt < 1:107278/149‑1 (MQ=255) aaaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTa > 2:830031/1‑149 (MQ=255) aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTAc < 2:512922/149‑1 (MQ=255) gagCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCGTGCTGAATGGCCTGCATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGGGTATAATCAGCATTACTc > 1:216635/3‑149 (MQ=255) ggTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCTAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa < 1:300343/149‑1 (MQ=255) ggTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTaa > 1:528806/1‑149 (MQ=255) cGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTAca < 1:346965/149‑1 (MQ=255) cGCTCTCGACGGCAACGGATGCAATAAGGCAGGATCCTTCGCCGAAGATCTCGTGAAAAGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGCCAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAca > 1:637735/1‑149 (MQ=255) ggCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCCGCATTACTCAGAAAATTAACGTTACAGCAGTATACg > 2:870719/1‑149 (MQ=255) gCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACgg > 1:382042/1‑149 (MQ=255) cAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGtt < 2:959324/134‑1 (MQ=255) cAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGtt > 1:959324/1‑134 (MQ=255) aTGCAATATTGAAGGAAGATTCGCCGTAGATATGGTGAATTGCCTGATGATTGGGCTGAATTTTAGCGTGATTGGTGGTCGCAGATACAACTTGGTGAATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGaaaaaaa < 2:944157/149‑1 (MQ=255) tGCAATATGGCAGGATCCTTCTCCGAAGATCTCGAGAAATGCCTGCAGAATGGCCTGAATTTTAGCGTTATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAg < 1:380515/149‑1 (MQ=255) gCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGc < 2:616641/149‑1 (MQ=255) aaTATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCAt > 1:517925/1‑149 (MQ=255) tatGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGt > 1:73781/1‑149 (MQ=255) aTCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATTGCCTGAATTTTAGCGGGTTTGGTGGGCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCATTATACGGAAAAAAAGCATGTTACAGGtat < 2:456848/149‑1 (MQ=255) ccTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACTGAAAAAAAGCATGTTACAGGTATAc < 1:800674/149‑1 (MQ=255) cTTCGCCGAAGATCTCGTGAAATTCCTGCAGAATGGCCTGAATAATAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGTTATAcc > 1:682706/1‑149 (MQ=255) ttCGCCGAAGATCTCGTGAAATGCCTGCAGAATGTCCTGAAATTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATCTTACAGGAATAcat > 1:1005338/1‑147 (MQ=255) tCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATAACTa > 1:924622/1‑149 (MQ=255) cGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGGTGTAGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAg < 2:246925/149‑1 (MQ=255) cGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTcc > 2:575446/1‑149 (MQ=255) aGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGcc < 2:447466/149‑1 (MQ=255) aTCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCAc > 1:3018/1‑149 (MQ=255) tctcGTGAAATGCCTGCAGAATGGCATGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAGAAAAAGCATGTTACAGGTATACCTAGTCCGCCACg < 1:860212/149‑1 (MQ=255) tcGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGt > 1:794410/1‑149 (MQ=255) tcGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGt < 1:455040/149‑1 (MQ=255) gTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGa < 1:878237/149‑1 (MQ=255) gTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGGTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGa < 1:656158/149‑1 (MQ=255) gAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTAGCACATAAAACTTTGTTCATATTCAGCATTACTGAGAAACTTAACTGTACAGGAGTATACGGATAAAAAGCATGTTACAGGTATACCTAGTACGCCTCGGTGaaa < 2:254931/149‑1 (MQ=255) cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTg > 1:141348/1‑149 (MQ=255) cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTg > 1:236988/1‑149 (MQ=255) cTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAATAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGCTATACCTAGTCCGACACGGTGAAAAGCactt > 1:586356/1‑146 (MQ=255) aGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAAc < 1:830031/149‑1 (MQ=255) gAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTACCGTTACAGCAGTATACGGAAACACAGCATGTTACAGCTATACCTAGTCCGCCACGGGGAACCGCAGTGGACCg > 1:334506/1‑149 (MQ=255) tGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCTTTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGCTATACCTAGTCCGACACTGAGAAACGCAGTGGACCGCCGAGCGa > 2:260110/1‑149 (MQ=255) taaTTTTCGCTGGATTGTTGGGTTAACAGAGAAATTGGTGTATCATGAGCTTATCTCAGAAAATTAACGTTACAGAAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCAGCCACGTTGAAACGCAGTGTAACGCCGAGCGAc < 2:965112/148‑1 (MQ=255) tAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTc > 1:564756/1‑149 (MQ=255) tAGCGGGATAGGTGGTCGCACAGAAAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGGTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTc < 1:414317/149‑1 (MQ=255) gCGGGATTGGTGGTCGCACAGACAACTTGGTGTATAATCAGCAATACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGATACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAg < 2:528806/149‑1 (MQ=255) gggATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATCCAggg < 1:870719/149‑1 (MQ=255) ttGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAg > 2:956506/1‑149 (MQ=255) ggtggtCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTc < 1:359482/149‑1 (MQ=255) aGACAACTTGGTGTATAATCAGCATTACTCAGAAAATTAACGATACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATAACTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGc > 2:207487/1‑149 (MQ=255) aaCTTGGTGTATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTCAAACGCAGTGTAACGCAGAGCGACGTATTCAGCGCCAGTCTGACAGCCCGCTGAc > 1:11482/1‑149 (MQ=255) | CCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGCATGTTACAGGTATACCTAGTCCGCCACGGTGAAACGCAGTGGAACGCCGAGCGACGTATTCAGGGCCAGTCTGACAGCCCGCTGAC > NC_000913/4633598‑4633882 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |