Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 659,204 C→T 7.1% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913659,2040CT 7.1% 182.3 / 4.6 71intergenic (+54/+47)tatE/lipATatABCE protein translocation system subunit/lipoate synthase
Reads supporting (aligned to +/- strand):  ref base C (40/25);  new base T (2/3);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

AAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGC  >  NC_000913/659056‑659352
                                                                                                                                                    |                                                                                                                                                    
aaGGGTTCAAGAAGGCGATGAATCATGACGATGCTGCGTCGAAAAAATGCGCAGACGTTGAAATTCAGGCTCAAAAGCTCTCTCAGAAAGAGTGACGTAGCGAGCAGGCCGCTCCCTCAATTACTTGTTCGATACAAACAACCAGCTTc                                                                                                                                                      >  2:666832/1‑149 (MQ=255)
 aGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACACGCTTCa                                                                                                                                                     >  2:136134/1‑149 (MQ=255)
    gTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCaaaa                                                                                                                                                  >  1:865282/1‑149 (MQ=255)
     ttCAAGAAGGCGAGGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTATCATAAAGAGTGACGTGGCTAGCACGACGCTCCCTCAAAATCTTGTTAGATAAAAAAAACCCGCTACaaaaa                                                                                                                                                 >  1:87283/1‑149 (MQ=255)
             ggCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttt                                                                                                                                         <  2:551919/149‑1 (MQ=255)
                gATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCGCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGtttttt                                                                                                                                      <  1:788104/149‑1 (MQ=255)
                 atgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttttttg                                                                                                                                     <  1:306767/149‑2 (MQ=255)
                 atgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTa                                                                                                                                     >  2:234702/1‑149 (MQ=255)
                          gaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATcagacaga                                                                                                                            >  1:360848/1‑149 (MQ=255)
                             gATGCTGCGGCGAAAAAAGGAGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCAGAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCGATAGCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgt                                                                                                                         >  1:599283/1‑149 (MQ=255)
                                    cggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAat                                                                                                                  >  2:428978/1‑149 (MQ=255)
                                     ggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAACGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTACGTAatt                                                                                                                 <  2:514403/149‑1 (MQ=255)
                                      gcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCGCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAatta                                                                                                                <  2:649981/149‑1 (MQ=255)
                                        gAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCCATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAagtaagtagt                                                                                                              >  2:740424/1‑147 (MQ=255)
                                            aaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAg                                                                                                          <  1:292272/149‑1 (MQ=255)
                                                  gcAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTAc                                                                                                    <  1:692063/149‑1 (MQ=255)
                                                    aGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACtt                                                                                                  <  1:648381/149‑1 (MQ=255)
                                                      aCGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTaa                                                                                                >  2:280689/1‑149 (MQ=255)
                                                         ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                             <  1:399404/149‑1 (MQ=255)
                                                           gATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGGTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTcc                                                                                           >  1:308440/1‑149 (MQ=255)
                                                            aTCTTCAGTCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                          <  1:239504/149‑1 (MQ=255)
                                                            aTCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                          <  1:243271/149‑1 (MQ=255)
                                                             tCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCAt                                                                                         <  2:459557/149‑1 (MQ=255)
                                                              cTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATc                                                                                        >  2:838542/1‑149 (MQ=255)
                                                               ttCAGGCTGAAAAGCTCTCTCATAAAGAGTGACTTTGCGAGCAGGACGGTGCATCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATAATTACTGGATTACTTAACTTCCATcc                                                                                       <  2:925192/149‑1 (MQ=255)
                                                                tCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACATAAAACCCGCTTCAAAACGCGGGTTATTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACATCCAtaac                                                                                      >  2:568473/1‑146 (MQ=255)
                                                                tCAGGATGAACATCTCGCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCATCAATATCTTGGTCGATACCAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATccc                                                                                      <  2:921775/149‑1 (MQ=255)
                                                                      tGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGc                                                                                >  1:410437/1‑149 (MQ=255)
                                                                       gAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGcc                                                                               >  1:826501/1‑149 (MQ=255)
                                                                        aaaaGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCt                                                                              >  1:79484/1‑149 (MQ=255)
                                                                                tctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGGTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCg                                                                      <  2:55303/149‑1 (MQ=255)
                                                                                    ttAAACAGTGACGTGGCGAGCATGACGCTCCCTCATTATCTTGTTCGATACTAAAAACCGGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAACTCGGCGt                                                                  <  2:960845/148‑1 (MQ=255)
                                                                                     tAAAGCGTGACGTGGCGAGCAGGACGCTCCCTCAATATCGTGTTCGATACAACAAACCCGCTTCAAAAAGCGGGTTTGTGAGCAGACAGATGTACGTAAGTATTCCAGGATTACGTACCTTCCATCCCTTTCGCCGGCACATCGGCggg                                                                 >  1:932709/1‑147 (MQ=255)
                                                                                     tAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGCTTACTTAACTTCAATCCCTTTCGCCTGCAAATCGGCGTg                                                                 >  1:737452/1‑149 (MQ=255)
                                                                                     tAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCATAACGCGGGTTTTTGATCAGACAGATGTAAGTAATTATTACTGTTTTACTTAACTTCCATCCCTTTCGCCTGCAATTGGGGGTg                                                                 >  2:986806/1‑149 (MQ=255)
                                                                                      aaaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCCACCCTTTCGCCTCCAAATCGGCGTgg                                                                >  1:550862/1‑149 (MQ=255)
                                                                                       aaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCCGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCAACCCTTTCGCCTGCAAATCGGCGTGGt                                                               >  1:868172/1‑149 (MQ=255)
                                                                                         gagTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaa                                                             <  2:454722/149‑1 (MQ=255)
                                                                                              aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAagaaga                                                        >  1:835642/1‑149 (MQ=255)
                                                                                              aCGTCGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATCTAAGAAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGAAagaaga                                                        >  2:887549/1‑149 (MQ=255)
                                                                                               cggggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAagag                                                       <  2:699345/146‑1 (MQ=255)
                                                                                                gTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGc                                                      <  2:435552/149‑1 (MQ=255)
                                                                                                  ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCAATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCgg                                                    >  2:455913/1‑149 (MQ=255)
                                                                                                   gCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGa                                                   <  1:455913/149‑1 (MQ=255)
                                                                                                     gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACa                                                 >  1:592769/1‑149 (MQ=255)
                                                                                                     gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTGTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGAGGACa                                                 >  1:871292/1‑149 (MQ=255)
                                                                                                         aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACTGATGTAAGTAATGATTACCGGATTACTTATATTCCCGCCCTTTCGCGGGCACATCGGCGAGGTAATCAGAGCGGACACACg                                             >  2:337887/1‑149 (MQ=255)
                                                                                                           gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                           <  2:865282/149‑1 (MQ=255)
                                                                                                           gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                           >  1:41356/1‑149 (MQ=255)
                                                                                                             cGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGAGAAACGGAcc                                         >  2:197113/1‑149 (MQ=255)
                                                                                                              gCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTAAAGGATTAATTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCTGACAAACGGCCCg                                        >  2:711594/1‑149 (MQ=255)
                                                                                                                tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCa                                      >  2:309044/1‑149 (MQ=255)
                                                                                                                   cTCAATATCTTGTTCGATACAAAAAACCCGCTTCATAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgc                                   >  1:204864/1‑149 (MQ=255)
                                                                                                                      aaTATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAACCGGACCGCATgcagc                                >  1:817677/1‑149 (MQ=255)
                                                                                                                        tatCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCAt                              <  1:280689/149‑1 (MQ=255)
                                                                                                                         atCTTGTTCGATACAAAAAACCCGCTTCAAACAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATg                             <  2:589427/149‑1 (MQ=255)
                                                                                                                          tattGTTAGATACAAAAAACCCGCTTCAAAAAGCTGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                            <  2:871292/147‑1 (MQ=255)
                                                                                                                          tCTTGGTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                            <  2:249144/149‑1 (MQ=255)
                                                                                                                            ttGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATAAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGt                          >  2:765058/1‑149 (MQ=255)
                                                                                                                               ttCGATACAAAAAACCCGCTTAAAAACGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTaaa                       <  2:692347/149‑1 (MQ=255)
                                                                                                                                   tgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                   >  2:277327/5‑149 (MQ=255)
                                                                                                                                   aTACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                   <  2:300281/149‑1 (MQ=255)
                                                                                                                                   aTACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                   >  1:457640/1‑149 (MQ=255)
                                                                                                                                      taaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCAAGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAATCACATc                >  2:79923/2‑149 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATTCAGCATGGGTAAAGACCATCg               >  2:996302/1‑149 (MQ=255)
                                                                                                                                       aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCg               >  1:776666/1‑149 (MQ=255)
                                                                                                                                        aaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGc              >  2:543041/1‑149 (MQ=255)
                                                                                                                                           aaaccGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAg           <  1:234702/146‑1 (MQ=255)
                                                                                                                                            aCCCGCTTCGAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAAATATTACAGGATTACTTAACTTCCATCCCTTTCGCATGCAAATCGGCGTGGTAAGAAGAGCGGACAAACCGACCCCATGCAGCATGGGTAAAGCCCATCGCCCgc          >  1:193999/1‑149 (MQ=255)
                                                                                                                                                 cTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTc     >  2:297565/1‑149 (MQ=255)
                                                                                                                                                 cTTCAAAAAGCGGGTTTTTTACCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTc     >  2:573612/1‑149 (MQ=255)
                                                                                                                                                  ttCAAAAAGCGTGTTTTTAATCAGATAGATGTAAGTAATTATTTCAGGAGTACTTAAGTTCCACCCCTATCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCCGCATGGGTAAAGCCCATCGCCAGCGCTTCg    <  1:666832/149‑1 (MQ=255)
                                                                                                                                                    cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGc  <  2:1024050/149‑1 (MQ=255)
                                                                                                                                                    cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGAGCTTCGGc  >  1:458146/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGC  >  NC_000913/659056‑659352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑