Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 659,207 A→G 7.1% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913659,2070AG 7.1% 191.2 / 5.0 70intergenic (+57/+44)tatE/lipATatABCE protein translocation system subunit/lipoate synthase
Reads supporting (aligned to +/- strand):  ref base A (38/27);  new base G (2/3);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTT  >  NC_000913/659060‑659355
                                                                                                                                                   |                                                                                                                                                    
gTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCaaaa                                                                                                                                                     >  1:865282/1‑149 (MQ=255)
 ttCAAGAAGGCGAGGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTATCATAAAGAGTGACGTGGCTAGCACGACGCTCCCTCAAAATCTTGTTAGATAAAAAAAACCCGCTACaaaaa                                                                                                                                                    >  1:87283/1‑149 (MQ=255)
         ggCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttt                                                                                                                                            <  2:551919/149‑1 (MQ=255)
            gATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCGCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGtttttt                                                                                                                                         <  1:788104/149‑1 (MQ=255)
             atgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttttttg                                                                                                                                        <  1:306767/149‑2 (MQ=255)
             atgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTa                                                                                                                                        >  2:234702/1‑149 (MQ=255)
                      gaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATcagacaga                                                                                                                               >  1:360848/1‑149 (MQ=255)
                         gATGCTGCGGCGAAAAAAGGAGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCAGAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCGATAGCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgt                                                                                                                            >  1:599283/1‑149 (MQ=255)
                                cggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAat                                                                                                                     >  2:428978/1‑149 (MQ=255)
                                 ggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAACGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTACGTAatt                                                                                                                    <  2:514403/149‑1 (MQ=255)
                                  gcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCGCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAatta                                                                                                                   <  2:649981/149‑1 (MQ=255)
                                    gAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCCATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAagtaagtagt                                                                                                                 >  2:740424/1‑147 (MQ=255)
                                        aaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAg                                                                                                             <  1:292272/149‑1 (MQ=255)
                                              gcAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTAc                                                                                                       <  1:692063/149‑1 (MQ=255)
                                                aGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACtt                                                                                                     <  1:648381/149‑1 (MQ=255)
                                                  aCGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTaa                                                                                                   >  2:280689/1‑149 (MQ=255)
                                                     ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                                <  1:399404/149‑1 (MQ=255)
                                                       gATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGGTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTcc                                                                                              >  1:308440/1‑149 (MQ=255)
                                                        aTCTTCAGTCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                             <  1:239504/149‑1 (MQ=255)
                                                        aTCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                             <  1:243271/149‑1 (MQ=255)
                                                         tCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCAt                                                                                            <  2:459557/149‑1 (MQ=255)
                                                          cTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATc                                                                                           >  2:838542/1‑149 (MQ=255)
                                                           ttCAGGCTGAAAAGCTCTCTCATAAAGAGTGACTTTGCGAGCAGGACGGTGCATCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATAATTACTGGATTACTTAACTTCCATcc                                                                                          <  2:925192/149‑1 (MQ=255)
                                                            tCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACATAAAACCCGCTTCAAAACGCGGGTTATTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACATCCAtaac                                                                                         >  2:568473/1‑146 (MQ=255)
                                                            tCAGGATGAACATCTCGCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCATCAATATCTTGGTCGATACCAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATccc                                                                                         <  2:921775/149‑1 (MQ=255)
                                                                  tGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGc                                                                                   >  1:410437/1‑149 (MQ=255)
                                                                   gAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGcc                                                                                  >  1:826501/1‑149 (MQ=255)
                                                                    aaaaGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCt                                                                                 >  1:79484/1‑149 (MQ=255)
                                                                            tctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGGTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCg                                                                         <  2:55303/149‑1 (MQ=255)
                                                                                ttAAACAGTGACGTGGCGAGCATGACGCTCCCTCATTATCTTGTTCGATACTAAAAACCGGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAACTCGGCGt                                                                     <  2:960845/148‑1 (MQ=255)
                                                                                 tAAAGCGTGACGTGGCGAGCAGGACGCTCCCTCAATATCGTGTTCGATACAACAAACCCGCTTCAAAAAGCGGGTTTGTGAGCAGACAGATGTACGTAAGTATTCCAGGATTACGTACCTTCCATCCCTTTCGCCGGCACATCGGCggg                                                                    >  1:932709/1‑147 (MQ=255)
                                                                                 tAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGCTTACTTAACTTCAATCCCTTTCGCCTGCAAATCGGCGTg                                                                    >  1:737452/1‑149 (MQ=255)
                                                                                 tAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCATAACGCGGGTTTTTGATCAGACAGATGTAAGTAATTATTACTGTTTTACTTAACTTCCATCCCTTTCGCCTGCAATTGGGGGTg                                                                    >  2:986806/1‑149 (MQ=255)
                                                                                  aaaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCCACCCTTTCGCCTCCAAATCGGCGTgg                                                                   >  1:550862/1‑149 (MQ=255)
                                                                                   aaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCCGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCAACCCTTTCGCCTGCAAATCGGCGTGGt                                                                  >  1:868172/1‑149 (MQ=255)
                                                                                     gagTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaa                                                                <  2:454722/149‑1 (MQ=255)
                                                                                          aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAagaaga                                                           >  1:835642/1‑149 (MQ=255)
                                                                                          aCGTCGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATCTAAGAAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGAAagaaga                                                           >  2:887549/1‑149 (MQ=255)
                                                                                           cggggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAagag                                                          <  2:699345/146‑1 (MQ=255)
                                                                                            gTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGc                                                         <  2:435552/149‑1 (MQ=255)
                                                                                              ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCAATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCgg                                                       >  2:455913/1‑149 (MQ=255)
                                                                                               gCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGa                                                      <  1:455913/149‑1 (MQ=255)
                                                                                                 gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACa                                                    >  1:592769/1‑149 (MQ=255)
                                                                                                 gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTGTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGAGGACa                                                    >  1:871292/1‑149 (MQ=255)
                                                                                                     aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACTGATGTAAGTAATGATTACCGGATTACTTATATTCCCGCCCTTTCGCGGGCACATCGGCGAGGTAATCAGAGCGGACACACg                                                >  2:337887/1‑149 (MQ=255)
                                                                                                       gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                              >  1:41356/1‑149 (MQ=255)
                                                                                                       gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                              <  2:865282/149‑1 (MQ=255)
                                                                                                         cGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGAGAAACGGAcc                                            >  2:197113/1‑149 (MQ=255)
                                                                                                          gCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTAAAGGATTAATTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCTGACAAACGGCCCg                                           >  2:711594/1‑149 (MQ=255)
                                                                                                            tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCa                                         >  2:309044/1‑149 (MQ=255)
                                                                                                               cTCAATATCTTGTTCGATACAAAAAACCCGCTTCATAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgc                                      >  1:204864/1‑149 (MQ=255)
                                                                                                                  aaTATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAACCGGACCGCATgcagc                                   >  1:817677/1‑149 (MQ=255)
                                                                                                                    tatCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCAt                                 <  1:280689/149‑1 (MQ=255)
                                                                                                                     atCTTGTTCGATACAAAAAACCCGCTTCAAACAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATg                                <  2:589427/149‑1 (MQ=255)
                                                                                                                      tattGTTAGATACAAAAAACCCGCTTCAAAAAGCTGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                               <  2:871292/147‑1 (MQ=255)
                                                                                                                      tCTTGGTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                               <  2:249144/149‑1 (MQ=255)
                                                                                                                        ttGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATAAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGt                             >  2:765058/1‑149 (MQ=255)
                                                                                                                           ttCGATACAAAAAACCCGCTTAAAAACGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTaaa                          <  2:692347/149‑1 (MQ=255)
                                                                                                                               tgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                      >  2:277327/5‑149 (MQ=255)
                                                                                                                               aTACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                      <  2:300281/149‑1 (MQ=255)
                                                                                                                               aTACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                      >  1:457640/1‑149 (MQ=255)
                                                                                                                                  taaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCAAGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAATCACATc                   >  2:79923/2‑149 (MQ=255)
                                                                                                                                   aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATTCAGCATGGGTAAAGACCATCg                  >  2:996302/1‑149 (MQ=255)
                                                                                                                                   aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCg                  >  1:776666/1‑149 (MQ=255)
                                                                                                                                    aaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGc                 >  2:543041/1‑149 (MQ=255)
                                                                                                                                       aaaccGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAg              <  1:234702/146‑1 (MQ=255)
                                                                                                                                        aCCCGCTTCGAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAAATATTACAGGATTACTTAACTTCCATCCCTTTCGCATGCAAATCGGCGTGGTAAGAAGAGCGGACAAACCGACCCCATGCAGCATGGGTAAAGCCCATCGCCCgc             >  1:193999/1‑149 (MQ=255)
                                                                                                                                             cTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTc        >  2:297565/1‑149 (MQ=255)
                                                                                                                                             cTTCAAAAAGCGGGTTTTTTACCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTc        >  2:573612/1‑149 (MQ=255)
                                                                                                                                              ttCAAAAAGCGTGTTTTTAATCAGATAGATGTAAGTAATTATTTCAGGAGTACTTAAGTTCCACCCCTATCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCCGCATGGGTAAAGCCCATCGCCAGCGCTTCg       <  1:666832/149‑1 (MQ=255)
                                                                                                                                                cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGc     <  2:1024050/149‑1 (MQ=255)
                                                                                                                                                cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGAGCTTCGGc     >  1:458146/1‑149 (MQ=255)
                                                                                                                                                 aaaaaGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCt    >  2:368528/1‑149 (MQ=255)
                                                                                                                                                  aaaaGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCtt   <  1:309044/149‑1 (MQ=255)
                                                                                                                                                   aaaGGGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTATGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCttt  <  2:610060/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTT  >  NC_000913/659060‑659355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑