Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,062,893 A→G 10.6% S295P (TCG→CCG)  cbpA ← DnaK co‑chaperone; curved DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,062,8930AG10.6% 83.6 / 3.8 39S295P (TCG→CCG) cbpADnaK co‑chaperone; curved DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (15/17);  new base G (2/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

CGCGGTTCAACCACCCCCAAACCGACAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGC  >  NC_000913/1062749‑1063037
                                                                                                                                                 |                                                                                                                                                
cgcgGTTCAACCACCCCCAAACCGAAAATTTCATTCAACTCATCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGAGCAAACGACGACTgg                                                                                                                                              <  1:967376/149‑1 (MQ=255)
     ttCAACCACCCCCAAACCGACAATTTCATTCCACTCCGCTTCAGAGATGCCGGTATGCAGGCAAAAATTCGGTAATAGTAAAACTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGt                                                                                                                                          >  2:480403/1‑149 (MQ=255)
     ttCAACCACCCCCAAACCGACAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACAGTAACAATAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGGCTGGGCGTc                                                                                                                                         >  2:305336/1‑149 (MQ=255)
     ttCAACCACCCCCAAACCGACAATTTCATTCAACTCCTATTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCAACGTAACATTAGCAATTATGGTTTCCCCCAATCTTTACCTGGATCAAAAGACGGCTGGGCGta                                                                                                                                         >  2:301172/1‑148 (MQ=255)
        aaCCACCCCCAAACCGACAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAACGACGACTGGGCGTCTGc                                                                                                                                      <  1:913224/149‑1 (MQ=255)
         accaccCCCAAACCGCCAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTAACCGTAACATTCGCCAGTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGCCAGGGCGTCTGcc                                                                                                                                     >  1:197521/1‑149 (MQ=255)
          ccaccCCCAAACCGACAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTGTCCCCCAATCTTTACGTGGATCATAAGACGACTGGGCGTCTGGCa                                                                                                                                    >  2:679426/1‑149 (MQ=255)
           caccCCCAAACCGACAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑TAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAg                                                                                                                                   >  1:5366/1‑149 (MQ=255)
                  aacccGACAATTTCATTCAACTCCTCTTCAGAGATGCCGGAAAGCAGGC‑AAACTTCGCTAATAGTAAAATTCACCGTACCATAAGCCTTTATGCTGTCCCCCAATCTTTACGTGTATCAAAAGACGACTGGGCGTCTGCCAGTtgctgc                                                                                                                            <  2:971565/146‑1 (MQ=255)
                        aCAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGGAATAGTTAAAGTCAACGTAACATAAGCCATTATGCTTTCCCCCATTCGGTACGTGGATAAAAAGACTACTGGGCGTCTGACAGTTGCTGCCACAga                                                                                                                      >  2:1022706/1‑148 (MQ=255)
                          aaTTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAgcgc                                                                                                                    >  2:630420/1‑149 (MQ=255)
                            ttttATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCgg                                                                                                                  >  2:130262/1‑149 (MQ=255)
                                attcaACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAgt                                                                                                              >  1:876918/1‑149 (MQ=255)
                                         ctctTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATc                                                                                                     >  1:281235/1‑149 (MQ=255)
                                                 agaTGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcg                                                                                             >  2:739572/1‑149 (MQ=255)
                                                  gaTGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcgg                                                                                            >  1:308553/1‑149 (MQ=255)
                                                   ttGCCTGTATGCAGGC‑AAAATTCGGTAATAGTTAAAGTCACCGTAACATTAGCCATTATGCTTTCCCACAATCTTTACGTGGAGCAAAAGCCGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggc                                                                                           <  1:722334/148‑1 (MQ=255)
                                                   aTGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGGCATTATGCTTTCGCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggc                                                                                           <  2:506873/149‑1 (MQ=255)
                                                   aTGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggc                                                                                           <  1:814216/149‑1 (MQ=255)
                                                   aTGACGGTATGAAGGC‑AAAATTGGGTAATAGTAAAAGTCACCGTAACATTCGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTcggc                                                                                           <  2:884308/149‑1 (MQ=255)
                                                         gTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAAGCTTTACGTGGATCAAAAGACGCCTGGGCGTCTGCAAGTTGATGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGAATc                                                                                     >  2:747627/1‑149 (MQ=255)
                                                                 gC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGAtttt                                                                             <  1:42274/149‑1 (MQ=255)
                                                                    aaaaTTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCa                                                                           >  2:536892/1‑149 (MQ=255)
                                                                               aTAGTAAAAGCCACCGTAACATTCGCCATTATGCATTCCCCCAATCTTTACGGCGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATAc                                                                <  1:679426/149‑1 (MQ=255)
                                                                                   tAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAgcg                                                            >  1:883947/1‑147 (MQ=255)
                                                                                      aaGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGCCTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGCTTTCGGCGGCTACACGAGTTTCAGTACCGCATACAGATCGc                                                         >  1:379159/1‑149 (MQ=255)
                                                                                        gTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCg                                                       <  1:235771/149‑1 (MQ=255)
                                                                                               tAACATGAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGtt                                                >  1:947506/1‑149 (MQ=255)
                                                                                                   aTTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGtttttt                                            <  2:654620/149‑1 (MQ=255)
                                                                                                   aTTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGtttttt                                            <  2:780591/149‑1 (MQ=255)
                                                                                                                  ttCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCttt                             <  2:150150/149‑1 (MQ=255)
                                                                                                                   tCCCCGAATGTTTACGTGGTTCATAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGTCATCAAGATTTTCAGTACCGAATACTTATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTg                            <  1:1022706/149‑1 (MQ=255)
                                                                                                                            cTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAAcg                   <  2:552835/149‑1 (MQ=255)
                                                                                                                              ttccGTGGCGCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCTGCAGTGTTTTCATCCGGTTTCGCCGGCATCACGATTTTCAGTCCCGCATACAGAGCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAAcgcg                 <  2:684041/146‑1 (MQ=255)
                                                                                                                                   tGGATCAAAATACGGCTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGGCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATc            <  1:301172/149‑1 (MQ=255)
                                                                                                                                   gggATCAAAAGACGGCTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATc            <  1:305336/148‑1 (MQ=255)
                                                                                                                                      aTCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGtt         >  2:552370/1‑149 (MQ=255)
                                                                                                                                       tCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTg        <  2:947506/149‑1 (MQ=255)
                                                                                                                                           aaGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGAGGCAGTGTTTTCATCCGGATTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGACTCTTTTTTGGTCACCAGACCTTTGCCTTTAAAGCGCAATCGTTGCCCg    >  2:483377/1‑149 (MQ=255)
                                                                                                                                             gacgacTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGATGCCCTGc  >  1:691288/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCGGTTCAACCACCCCCAAACCGACAATTTCATTCAACTCCTCTTCAGAGATGCCGGTATGCAGGC‑AAAATTCGGTAATAGTAAAAGTCACCGTAACATTAGCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGC  >  NC_000913/1062749‑1063037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑