Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,449,807 | C→G | 8.8% | G515R (GGT→CGT) | tynA ← | tyramine oxidase, copper‑requiring |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,449,807 | 0 | C | G | 8.8% | 139.4 / 8.4 | 57 | G515R (GGT→CGT) | tynA | tyramine oxidase, copper‑requiring |
Reads supporting (aligned to +/- strand): ref base C (27/25); new base G (3/2); total (30/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01 |
GATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGC > NC_000913/1449660‑1449955 | gATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCg < 2:869341/149‑1 (MQ=255) gAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTGATCTTTCGCCGTCTCATCGTGCAGGGTTTTCGCTTTTACACCTTTCACCGCTTCGATGCCCGTAGAACCGGCAt > 1:583391/1‑149 (MQ=255) ggCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCg < 1:597784/149‑1 (MQ=255) gCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGa < 1:246583/149‑1 (MQ=255) ataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCCTCTTTCGCCGGCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg > 2:977535/1‑149 (MQ=255) ataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTAGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg > 1:255927/1‑149 (MQ=255) aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGTTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGAATCGATGCCAATAGTGc > 1:322107/1‑149 (MQ=255) tgtgtgtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCAGGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGtt < 1:709572/144‑1 (MQ=255) tGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTCGCGCGGGTCATCTTTCGCCGTCTCATCGTGCTTGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTc < 1:493177/149‑1 (MQ=255) gtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGACGTTTTCAt > 2:37243/1‑149 (MQ=255) tgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGa > 2:773969/1‑149 (MQ=255) gtaCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGAt > 2:89891/1‑149 (MQ=255) cccACGATATTGTTATCGATACGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGATTTCGCTTTAACAACTTTCACCGCTTCGATTCCCGTAGAACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa < 1:836596/149‑1 (MQ=255) cccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa > 2:73499/1‑149 (MQ=255) cccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTATAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa > 2:876986/1‑149 (MQ=255) ccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAg < 2:990656/149‑1 (MQ=255) cACGATTTTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCCTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGt < 1:361611/149‑1 (MQ=255) cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTACCACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGt > 1:987179/1‑149 (MQ=255) cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCTTCGATGCCAATAGGGCCGTTTTCATGGAAGATCCAGt > 2:116923/1‑149 (MQ=255) aCGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGACATCTTTCGCCGTCGCAGCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCCCCGGCATCGATGCCAATAGTGACGTTTTAATGGAAGATcaattc > 1:979160/1‑144 (MQ=255) gATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAg < 1:952213/149‑1 (MQ=255) aTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt > 1:710428/1‑149 (MQ=255) tCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCCTCGTGCATGGTTTTCGCTTTAACACCTTTCACCGGTTCGATGCCCGTAGCACCGGCATCGATGGCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTc < 1:936917/149‑1 (MQ=255) cGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCAcg > 1:141272/1‑148 (MQ=255) cgcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACt < 1:119153/149‑1 (MQ=255) cgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGa < 2:88696/149‑1 (MQ=255) tCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGGCAAAAATGTAGTCACAGTTACCCACTGTACTGATccc > 2:234359/1‑148 (MQ=255) cATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAg < 1:73499/149‑1 (MQ=255) aTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgc < 1:338906/149‑1 (MQ=255) aTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCAAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAGTGATCCAgc > 2:349683/1‑149 (MQ=255) aTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGAAGCCCGTAGCACCGGCAGCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGGAGTCATAGTTACCCACTGTACTGATCCAgc < 1:206367/149‑1 (MQ=255) aTCTTTCGCCGTCTCATCGAGCATGGTTTTCGCTTTAACACCTTACACCGCTTCTATGCCCGTAGCCCCGGCATCGATGCCAATAGTGACGTTGTCATGGAAGATACAGTCTAAAATGTAGTCATAGTTACCCACAGATCTGATCCAgc > 1:294715/1‑149 (MQ=255) cTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGCTGCCAATAGTGCGGTTTTCATGGAAGATCCAGTCAAAAATGTAGACATAGTTACCCACTGTACTGATCCAgcgc > 2:713444/1‑149 (MQ=255) cTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgcgc < 1:37243/149‑1 (MQ=255) tttCGCCGTCTCATCGTGCAGGGATATCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGca < 2:853894/149‑1 (MQ=255) ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcac < 2:652368/149‑1 (MQ=255) cGCCGTATCATCGTTCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacca < 1:321789/149‑1 (MQ=255) cATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCGCGTAGCACCGGCATCGATGCCAATTGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAATGCTCCAGCGCACCACTAACTCCCGGCGtt > 1:280521/1‑149 (MQ=255) tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCg > 2:182393/1‑149 (MQ=255) tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCg < 1:471768/149‑1 (MQ=255) ttttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGCTGCCAATAGTGCCGTTTTCAAGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACAGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTa > 2:322295/1‑149 (MQ=255) tCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTg > 2:797578/1‑149 (MQ=255) gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc < 1:952682/149‑1 (MQ=255) gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCAGTTTTCATGGAAGATCCAGTCAAAATTGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACGAACTCCCGGCGGTCGGTACTGAc > 2:425675/1‑149 (MQ=255) aaCACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTgg > 2:659789/1‑149 (MQ=255) cTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTgg > 2:449415/1‑149 (MQ=255) tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTGCAGGCCTTCGGTACTGACTTTGGGCTGGc > 2:200231/1‑149 (MQ=255) tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc > 2:872038/1‑138 (MQ=255) tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc < 1:872038/138‑1 (MQ=255) cTTCCATGCCCGTAGCACCTGCATCGATGCCAATCTTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTATTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc < 1:208644/149‑1 (MQ=255) atCGATGCCGGTGCTACGGGCATCGCTGCCAATAGTGGCTTTTTCATGGAAGATCCAGTCAAAAATGTAGACAGAGTACCCCACTGTACTTAACCAGCGCACCACAACCACCCGGCCGTCCCTACAGACTATGGGCTGGCCCATTTCCt > 1:569575/2‑149 (MQ=255) atCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt > 1:895513/2‑149 (MQ=255) atCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt > 2:394626/2‑149 (MQ=255) tCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACAAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTg < 2:858607/149‑1 (MQ=255) aTGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTGATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATg < 2:500008/149‑1 (MQ=255) ccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta < 1:660345/149‑1 (MQ=255) tAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATAc < 1:773969/149‑1 (MQ=255) ccGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCCCTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTccgg > 1:751893/1‑149 (MQ=255) cGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTccggc > 2:42949/1‑149 (MQ=255) cGGAATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATTGATACCCACTCTACTGATCCCGCGCACCACTAACTCACGGCGTTAGGTACTAACGTTTGGATGGCCCCTATCGTGATGCTTATAATccgtc > 2:579096/1‑147 (MQ=255) | GATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGC > NC_000913/1449660‑1449955 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |