Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,460,565 G→A 6.2% G32D (GGC→GAC)  paaI → hydroxyphenylacetyl‑CoA thioesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,460,5650GA 6.2% 182.0 / 2.9 64G32D (GGC→GAC) paaIhydroxyphenylacetyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base G (35/25);  new base A (2/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

AACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCA  >  NC_000913/1460417‑1460711
                                                                                                                                                    |                                                                                                                                                  
aaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAgg                                                                                                                                                    <  2:288263/149‑1 (MQ=255)
     tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTg                                                                                                                                               <  2:830429/149‑1 (MQ=255)
          cccATGTTCATTGCTGCGCCAACTGGCGCTTCTGGAGAGCGGTTATGAGTCATAATGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTa                                                                                                                                          <  1:912774/149‑1 (MQ=255)
              tGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGa                                                                                                                                      <  1:851021/149‑1 (MQ=255)
                ttCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                                                                                                                                    >  1:736345/1‑149 (MQ=255)
                ttCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                                                                                                                                    >  2:202619/1‑149 (MQ=255)
                ttCATTGCTGCGCCAACGGGCGCTTCTGGAGAGAGGTTATGAGTCATTAGGCCTGGCAATATGCCCATGCAATGTATGAGAACGATGCCTGCGCCTAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                                                                                                                                    >  2:901318/1‑149 (MQ=255)
                   aTTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATg                                                                                                                                 >  1:293084/1‑149 (MQ=255)
                    ttGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCAACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGa                                                                                                                                >  2:813093/1‑149 (MQ=255)
                      gctgcGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGAcc                                                                                                                              >  2:803097/1‑149 (MQ=255)
                       ctgcGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCg                                                                                                                             >  1:849204/1‑149 (MQ=255)
                        tgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCga                                                                                                                            >  2:170175/1‑148 (MQ=255)
                           gcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAACGCGCTTGGCATCGACATTATCTCAATGGATGAAGACTTTGCTGTAGTGACCATGACCGTCAc                                                                                                                         >  2:692597/1‑149 (MQ=255)
                           gcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGACTTTGCTGTAGTGACCATGACCGTCAc                                                                                                                         >  2:67667/1‑149 (MQ=255)
                                       ttCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGTCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCt                                                                                                             >  2:103646/1‑149 (MQ=255)
                                       ttCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCt                                                                                                             >  2:729944/1‑149 (MQ=255)
                                       ttCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCt                                                                                                             >  2:73385/1‑149 (MQ=255)
                                          tGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATTCCTGCGCCAAAGCGCTTTGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTTACCATGACCGTCACTGAACAAAAGCTTaa                                                                                                          >  1:472793/1‑149 (MQ=255)
                                           ggAGAGCGGATATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTGGGCATCGACATTATCTCAATGGAAGAAGGCTTTGCTGTAGTGACCATGACCGTCAGTGCACAAATGCTTAAc                                                                                                         >  2:125041/1‑149 (MQ=255)
                                               cgCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGtc                                                                                                     <  1:423975/148‑1 (MQ=255)
                                                gCGGATATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGtca                                                                                                    <  1:1001943/149‑1 (MQ=255)
                                                  ggTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGAACGTCACTGCACAAATGCTTAACGGtcatc                                                                                                  >  1:131938/1‑149 (MQ=255)
                                                    ttttGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCaa                                                                                                >  1:909942/4‑149 (MQ=255)
                                                       tGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGt                                                                                             >  2:305757/1‑149 (MQ=255)
                                                        gAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCAAAAGCGCTTGGCATCGACATTATCTCAATGGAGGAAGGCTTTGCTGTAGTGACCCTGACCGTCAATGCACAAATTCTTATCGGTCATCAAAGta                                                                                            >  1:691264/1‑148 (MQ=255)
                                                             aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCAc                                                                                       >  2:307564/1‑149 (MQ=255)
                                                               aaGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGAGACCATGACCGTCACTGCACAAATGCTTAACGGTCAACAAAGTTGCCAcgg                                                                                     >  2:856732/1‑149 (MQ=255)
                                                                  gCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCAcggcgg                                                                                  <  1:339202/149‑1 (MQ=255)
                                                                    cTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATAATCTCAAAGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACCGTCCTCAAAGTTGCCACGGCGGGc                                                                                >  1:24060/1‑149 (MQ=255)
                                                                         aaaaTGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCAAAACTGCTTAACGGTCATCAAATTTGCCGCGGCGGGCAGCTa                                                                           >  2:608225/1‑149 (MQ=255)
                                                                              gCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTc                                                                      >  2:373553/1‑149 (MQ=255)
                                                                                ccATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCAc                                                                    >  2:471630/1‑149 (MQ=255)
                                                                                 cATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACt                                                                   >  2:203355/1‑149 (MQ=255)
                                                                                   tGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTA‑TTTCACTGGc                                                                <  1:100711/149‑1 (MQ=255)
                                                                                    gCAATGTATGAGAACGATGCCTGCGCCAAAGCGCGTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGc                                                                <  1:240030/149‑1 (MQ=255)
                                                                                    ccAATGTATCAGAACGATGCCTGAGCCAATGCGCTTGGCATCGACTATATCTCAATGGATGAAGGCGTTTCTGTAGTGACCATGACCGTCACTGGACATATGCTTAAGGGTCATCAAAGTTTCCACGGCGGGCAGCTATTTTCACTGGc                                                                <  1:584883/148‑1 (MQ=255)
                                                                                     ccaTGTAGGAGACCGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGAGGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCt                                                               <  1:950842/147‑1 (MQ=255)
                                                                                        tGTATGAGTACCATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGAt                                                            <  2:224692/149‑1 (MQ=255)
                                                                                           aTGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACt                                                         >  1:524379/1‑149 (MQ=255)
                                                                                            tGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCTCTGGCTGATACTg                                                        >  1:755446/1‑149 (MQ=255)
                                                                                                  cGATGTCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTg                                                  <  1:103646/149‑1 (MQ=255)
                                                                                                  agATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTTACCTTGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCTGCTATTTTCAGTGGCTGATACTGCCTTTg                                                  <  1:227778/148‑1 (MQ=255)
                                                                                                   gATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCCATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGGCATCAAAGGTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGc                                                 <  2:895302/149‑1 (MQ=255)
                                                                                                   gATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGc                                                 >  1:896509/1‑149 (MQ=255)
                                                                                                   gATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGc                                                 <  1:374245/149‑1 (MQ=255)
                                                                                                         tGCTCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGGCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGc                                           <  2:21093/149‑1 (MQ=255)
                                                                                                            gcCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTg                                        <  1:202619/149‑1 (MQ=255)
                                                                                                               aaaGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCAATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCaa                                     >  1:948784/1‑149 (MQ=255)
                                                                                                                 aGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATa                                   >  2:660703/1‑149 (MQ=255)
                                                                                                                      ttGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAg                              >  1:14737/1‑149 (MQ=255)
                                                                                                                       tGGCATCGACATTATCTCAATGGATGAAGACTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAgg                             <  1:67667/149‑1 (MQ=255)
                                                                                                                       gggCATCGACATTATCTCAATGGATGAAGACTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAgg                             <  1:692597/148‑1 (MQ=255)
                                                                                                                           aTCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCCCTGCACAAATGCTTAACGTTCATCAAAGTTGCCACGGCCGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCt                         >  1:713114/1‑149 (MQ=255)
                                                                                                                                cATTATCTCAATGGATTAAGGCTTTGCTGTAGTGACCAAGACCGTCACTGCACAAAATCTTAACGGTGATCAAAGTTGACACTGAGAGCAGCAATTTTAACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGccg                    >  2:284800/1‑147 (MQ=255)
                                                                                                                                  ttATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGcc                  <  2:293084/149‑1 (MQ=255)
                                                                                                                                  ttATCTCAATGGAGGAAGGCTTTGCTGTAGTGACCCTGACCGGCACTGCACAAATGCTTAACGGTCAGCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGcc                  <  1:59236/149‑1 (MQ=255)
                                                                                                                                     actcAATGGCTGAAGGCGTTGCTGAAGCGACCATGACCGTCACTGCACAAATGCGTAACGGGCATCAACGTTGCCCCGGCCGGCAGCTAGTTTCACTCGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCCGGGGCTGGCAGCCGTc               <  2:193304/148‑1 (MQ=255)
                                                                                                                                      ctcAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCGGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCg              <  1:729944/149‑1 (MQ=255)
                                                                                                                                       tcAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATCCTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGc             >  2:708636/1‑149 (MQ=255)
                                                                                                                                         aaTGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCtt           >  2:766986/1‑149 (MQ=255)
                                                                                                                                          ttgtaTGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACTACTGCTCAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCGGTTACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTc          <  2:725641/145‑1 (MQ=255)
                                                                                                                                           tGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTct         <  2:322052/149‑1 (MQ=255)
                                                                                                                                             gATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCGTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTctgc       <  1:40366/149‑1 (MQ=255)
                                                                                                                                                gAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTctgcctg    >  1:402278/1‑149 (MQ=255)
                                                                                                                                                  aGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCa  <  1:766986/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCAAAGTTGCCACGGCGGGCAGCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCA  >  NC_000913/1460417‑1460711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑