Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,992,083 G→C 5.2% noncoding (35/76 nt) glyW ← tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,992,0830GC 5.2% 194.9 / 2.2 70noncoding (35/76 nt)glyWtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (28/38);  new base C (2/2);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTC  >  NC_000913/1991939‑1992229
                                                                                                                                                |                                                                                                                                                  
ggACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTAAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAg                                                                                                                                                <  1:689791/149‑1 (MQ=255)
      gACGGATTTGCAATCCGCTACATAACAGCTTTGATAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACACGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                          >  2:390744/1‑149 (MQ=255)
      gACGGATTTGCAATCCGATACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                          <  1:935740/149‑1 (MQ=255)
       ccGGATTTGCAATCCGCTACATAACCGCTTTCTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACAGCCGCTTGCCGCCGATGTCTTTTAAACTGCAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgggg                                                                                                                                         <  2:707152/148‑4 (MQ=38)
         ggATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                       >  2:564015/1‑149 (MQ=255)
         ggATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                       <  2:471143/149‑1 (MQ=255)
         ggATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                       >  2:354785/1‑149 (MQ=255)
          gATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctct                                                                                                                                      <  2:15213/149‑1 (MQ=255)
          gATTTGCAATCCGCTACATAACCGCGTTGTTAACGCCCCAAATTCTTCAGCCCTTTCAGCCTGACTTCCCCTTGACGCCGATGTCTTTTAAACTGGCGCGGGAACCGAGACTCTAACTCGCGCCCCCGACCTTGCCAAGGTCggggtcg                                                                                                                                      <  2:305280/149‑7 (MQ=255)
           aTTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAACCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTa                                                                                                                                     <  1:205126/149‑1 (MQ=255)
           aTTTGCAATCCGCTACATAACCGATTTGTTAACGCGCCAAATTCTTCCGGCCTTTCATCCAGAAATCCGCTTGACGCCGATGTCTTTTAAACTGTAGCGGGAAACGAGACTCGAACTCGAGACCCCGACCTTGGCAAGGTCGTGCTCTa                                                                                                                                     <  2:612544/149‑1 (MQ=38)
            tttGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGTCGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                                                                                    <  1:705890/149‑1 (MQ=255)
              tGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGTCGCGGATGTCTTTTAAACTGGAGCGGGATCCGAGACTCGAACTCGCGACCCCGAGCTTGGCAAGGTCGTGGTCTACCa                                                                                                                                  >  1:692408/1‑149 (MQ=38)
               gccaTCCGCTACATACCCGCTTTGTTAAGGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTGTTTTAAACTGGAGCGGGAAACGAGTCTCGAACACGCGACCCCGAACTTGGCAAGGTCGTGCTCTACCac                                                                                                                                 >  2:797680/4‑148 (MQ=37)
               gCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCATACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa                                                                                                                                 <  2:758250/149‑1 (MQ=255)
               gCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACaaa                                                                                                                                 >  1:989072/1‑149 (MQ=255)
                cAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGCCGCCGATGTCTTTTAAACTGGAGCGGGCAACGCGACTCGCACTCGCGCCCCCGACCTTGGCAAGGTCGGGctcgagcacc                                                                                                                                >  1:929900/1‑142 (MQ=255)
                 aaTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGCTGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACACCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                               <  2:579019/149‑1 (MQ=255)
                 aaTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                               >  1:620209/1‑149 (MQ=255)
                   tCCGCTACATAACCGCTTTGTTAACGCGCCAAAGTCTTCATGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGa                                                                                                                             <  2:998847/149‑1 (MQ=255)
                    ccGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                            <  1:730932/149‑1 (MQ=255)
                    ccGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                            <  1:93437/149‑1 (MQ=255)
                      gCTACATACCCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAACCGAGACTCGATCTCGCGACCCCGACCGTGGCAAGGTCGTCCTCTACCAACTGAGCt                                                                                                                          >  1:268355/1‑149 (MQ=255)
                           aTAACCGCTTTTTTAACGCGCCAAAATCTTCAGGCATTTCAGCAAGACATCCGCTTGACGCCGATGTCATTTAAACTGGAGCGGGAAACGAGAATGCAACTCACGACCCCGAACTTGGCAAGGTCGTGCTCTACCCACTGTGCGATTcc                                                                                                                     >  2:689800/1‑149 (MQ=25)
                            tAACCGCTTTGTTTAGGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                    <  2:643834/149‑1 (MQ=255)
                            tAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                    >  1:629974/1‑149 (MQ=255)
                                 gCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCACCGGCGCTATTCCCGCAtt                                                                                                               >  1:604782/1‑149 (MQ=255)
                                 gCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGCGCTATTCCCGCAtt                                                                                                               >  2:969904/1‑149 (MQ=255)
                                 gCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAtt                                                                                                               >  1:594848/1‑149 (MQ=255)
                                 gCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCGATTCCCGCAtt                                                                                                               >  1:591844/1‑149 (MQ=255)
                                      gTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcatca                                                                                                          <  2:746973/149‑1 (MQ=255)
                                       ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCaa                                                                                                         <  2:147835/149‑1 (MQ=255)
                                          aCGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCa                                                                                                      >  2:655482/1‑149 (MQ=255)
                                               ccAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGCGCTATTCCCGCAGACATCACGCACTCAg                                                                                                 >  1:706326/1‑149 (MQ=25)
                                                cAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGt                                                                                                <  1:291048/149‑1 (MQ=255)
                                                           ggCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGa                                                                                     >  1:63139/1‑149 (MQ=255)
                                                            gCCGTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAt                                                                                    <  2:768862/149‑1 (MQ=255)
                                                             ccTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAtt                                                                                   <  1:564015/149‑1 (MQ=255)
                                                                ttCAGCCAGACATCCGCTTGACGCCGATGTCTTTTATACTGGAGCGGGAAACGAGACTCGACCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTCCCAACTGAGCTATTCACGCAGTCATCAAGCAAGCAGTTAAGCACTTGCTTtta                                                                                >  2:321662/1‑149 (MQ=25)
                                                                ttCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTtta                                                                                <  1:409925/149‑1 (MQ=255)
                                                                ttCAGCCAGACATCCGCTTGACGACGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGATAATCACTTGATTtta                                                                                <  2:752430/149‑1 (MQ=255)
                                                                   taaaaGACATCGGCGTCAAGCGGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCTATCAGTTAATCACTTGATTttattt                                                                             >  2:850940/5‑148 (MQ=21)
                                                                    gCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTtattat                                                                            <  1:833638/149‑1 (MQ=255)
                                                                    gCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCGACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTtattat                                                                            >  2:833319/1‑149 (MQ=255)
                                                                     ccAGACATCCGCTTGACGACGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAACCAATCAGTTAATCACTTGATTTTATTATc                                                                           <  2:797217/149‑1 (MQ=255)
                                                                     ccAGACATCCGCGTGACGCCGATGTCTTTTAAACTGGAGCGGGAACCGAGACTCGATCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCCACTGAGCTATTCCCGCCTTCATCAAGCCAGCAGTTATTCGCTTGAGTTTATTATc                                                                           >  1:578393/1‑149 (MQ=21)
                                                                         aCATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAACCGAGACTCGACCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCt                                                                       <  2:881582/149‑1 (MQ=255)
                                                                          cATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTg                                                                      <  1:340296/149‑1 (MQ=255)
                                                                                   gaagCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGATAATCACTTGATTTAATTATCGTCTGGCAATCAGt                                                             <  2:681900/146‑1 (MQ=255)
                                                                                   gACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGt                                                             >  1:638471/1‑149 (MQ=255)
                                                                                   gACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGt                                                             <  1:258127/149‑1 (MQ=255)
                                                                                     cGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgc                                                           <  1:854184/149‑1 (MQ=255)
                                                                                      gCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgcc                                                          >  1:409419/1‑149 (MQ=255)
                                                                                                     aCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGtt                                           <  2:653036/149‑1 (MQ=255)
                                                                                                     aCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGtt                                           <  1:969302/149‑1 (MQ=255)
                                                                                                     aCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGGCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGtt                                           <  1:746339/149‑1 (MQ=255)
                                                                                                             gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTTCCAAATGAGCTATTGCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTa                                   >  2:565061/1‑149 (MQ=255)
                                                                                                               gAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACt                                 <  1:354785/149‑1 (MQ=255)
                                                                                                                     agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGAAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTg                           <  1:972949/149‑1 (MQ=255)
                                                                                                                            aaCTCTCGACCCCGACCTTGGAAAGGTCGAGATCTACCAACTGAGCTATTCCCGCATTCATCAAGCCATCAGTTAATCACTTGATTTTATTATCGTCTGTCAATCAGTGCAGACGTTCGATGCTTTGCATTCTACTTACCTGGCGCGAt                    <  2:206688/149‑1 (MQ=37)
                                                                                                                            aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGAt                    <  2:203412/149‑1 (MQ=255)
                                                                                                                            aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTAGTATCGTCTGGCAATCAGTGCCGCCGTTCGCTGCGTTGCATTCTGCTTACCTGGCGCGAt                    >  1:1009140/1‑149 (MQ=255)
                                                                                                                            aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGATATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGAt                    <  1:390744/149‑1 (MQ=255)
                                                                                                                                cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGt                <  1:555828/149‑1 (MQ=255)
                                                                                                                                   gagcacGACCTTGCCAAGGTCGTGCTCTACCACCTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCaa             >  1:42151/6‑149 (MQ=255)
                                                                                                                                    agcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAAc            >  1:102687/5‑149 (MQ=255)
                                                                                                                                           ccTTGGCAAGGTCGTGCTCTACCAACTGAGCTTTTCCCGCATTCATCATGCAATCTGTTAATCACTTGATTTTATTATCGTCTGGCTATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATAttt     <  2:929900/149‑1 (MQ=255)
                                                                                                                                           ccTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGAGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATAttt     <  1:547598/149‑1 (MQ=255)
                                                                                                                                            cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGAATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATAtttt    >  2:727036/1‑149 (MQ=255)
                                                                                                                                              tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTc  >  2:634985/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTC  >  NC_000913/1991939‑1992229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑