Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA100.0% 147.4 / NA 54V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/31);  total (23/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACAC  >  NC_000913/2019316‑2019601
                                                                                                                                                |                                                                                                                                             
aGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGCCGATACGCTTCCGGTTCCCAtctc                                                                                                                                           <  1:356628/149‑1 (MQ=255)
 gTGGCCTTCAGTTATGGCAAAGCAACATCCGTGGCGAAAGCCTTAGTGGTCCGCAGCAGGCCGCTTCCCAGCATCAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATctct                                                                                                                                          <  2:984667/149‑1 (MQ=255)
    gCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                                                                       <  1:475685/149‑1 (MQ=255)
     cATTCAGTTAGGGCAAAGCAACATCTCTGGCGAACGCTGTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                                      <  2:794434/149‑1 (MQ=255)
      aTTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAACCCATGAACCTCTGGCGGGGGAAGACGCCGCTACGCTTCCGGTGACCATCTCTGTACa                                                                                                                                     >  2:186581/1‑149 (MQ=255)
           gTTAGGCCAAAGCAACATCAGTGGCGAATGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCTACGCACAGCAAACCATGAACCTCTGGCGGGGGATGACTACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                                                                <  2:154994/149‑1 (MQ=255)
            ttAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGCACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                               >  1:962431/1‑149 (MQ=255)
               gggCAAAGCAACATCAGTGGCTAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                                                                            <  1:165001/149‑1 (MQ=255)
                 gCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                          >  2:552553/1‑149 (MQ=255)
                  cAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAAc                                                                                                                         >  1:291294/1‑149 (MQ=255)
                   aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACa                                                                                                                        <  2:189560/149‑1 (MQ=255)
                      ccAACATCAGTGGCCTAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCTACTCCAACGCACACCAAACCATCAACCTCTCGCGGGGGAAGACGACGATACTCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                                     <  2:1008727/148‑1 (MQ=255)
                         aCATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAAc                                                                                                                  >  1:637605/1‑149 (MQ=255)
                           aTCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                                >  2:137278/1‑149 (MQ=255)
                           aTCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCGTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                                >  1:836986/1‑149 (MQ=255)
                                  gCGAAAGCTTTAGTGGTCAGCACCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGAAGACCATACGCTTCCGGTTCCAATCTCTTTACAAGGGAGGGTAACAGGCAACAgcggcgat                                                                                                         >  2:445230/1‑147 (MQ=255)
                                    gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                       <  1:14271/149‑1 (MQ=255)
                                        gcgttAGAGGTCAGCAGAAGGCCGCTTCCCAGCAACAGCAAAGCCAAAGCACAGAAAACCATTCACCTCACGCGGGGGAAGACGACTATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                                                                   <  1:562281/146‑1 (MQ=255)
                                         cTTTAGTGTTCAGCAGCAGGCCGCTTCCCAGCAAGAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTCCAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                                                                                  <  1:21252/149‑1 (MQ=255)
                                             aGTGGTCATCAGCAGGACGCTTCCCAGCAACATCAAAGACAACTCAGATCAAACCATGAACCTCTTGCTGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                              <  1:662115/149‑1 (MQ=255)
                                             aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGACGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                              <  2:1022977/149‑1 (MQ=255)
                                             aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACTAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                              <  2:165715/149‑1 (MQ=255)
                                                  tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAACCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                                                                                         >  1:135699/1‑149 (MQ=255)
                                                   cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCCAACCAAGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATTTTTTCGCCTACCg                                                                                        >  1:164790/1‑149 (MQ=255)
                                                            gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGtt                                                                               >  1:391716/1‑148 (MQ=255)
                                                             ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg                                                                              >  1:152646/1‑149 (MQ=255)
                                                              cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTACCGTCAGAGGTAGc                                                                             >  2:872721/1‑149 (MQ=255)
                                                              cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTACCGTCAGAGGTAGc                                                                             >  1:258444/1‑149 (MQ=255)
                                                              cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGc                                                                             >  1:550642/1‑149 (MQ=255)
                                                              cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCACATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGc                                                                             >  2:868890/1‑149 (MQ=255)
                                                                 ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGCTATTTTCGCCTAAGGTCAGAGGTAGCAcc                                                                          >  1:812442/1‑149 (MQ=255)
                                                                  tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTGACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAGTTTCGCCTACCGTCAGAGGTAGCACCg                                                                         >  1:649811/1‑149 (MQ=255)
                                                                    ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGACAGAGGTAGCACCGTa                                                                       >  1:1015704/1‑149 (MQ=255)
                                                                      agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt                                                                     >  2:292750/1‑149 (MQ=255)
                                                                          acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                 <  1:818343/149‑1 (MQ=255)
                                                                          acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                 <  1:484660/149‑1 (MQ=255)
                                                                             gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTc                                                              >  2:82220/1‑149 (MQ=255)
                                                                                  gCCAACGTACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                         <  2:165994/149‑1 (MQ=255)
                                                                                  gCCAACGCACAGCAAACCATGAACCTCGGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCATCAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                         <  1:445230/149‑1 (MQ=255)
                                                                                     aaagCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGTTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGAATAACGTCTGAGGTAGCACCGTAATCCGCGGCTTTTcccc                                                      <  1:198387/146‑1 (MQ=255)
                                                                                           caGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttg                                                <  2:265355/149‑1 (MQ=255)
                                                                                           caGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttg                                                <  2:544809/149‑1 (MQ=255)
                                                                                                     tGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGCTCCCATCTCTTTACAAGGGCGGGTAACAGGCAACAGCGGCGGTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCaa                                      <  2:159637/149‑1 (MQ=255)
                                                                                                           tctGGCGGGGTAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                                <  2:546201/149‑1 (MQ=255)
                                                                                                                   gggAAGACGACTATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAAtt                        >  2:101576/1‑149 (MQ=255)
                                                                                                                    ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTa                       <  2:43642/149‑1 (MQ=255)
                                                                                                                     gAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCACGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAg                      <  2:550642/149‑1 (MQ=255)
                                                                                                                         acgactATCCGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCGAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGCCGCAGGATAATTAGcctc                  >  2:90720/1‑147 (MQ=255)
                                                                                                                             cgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCGCAAGACGCAGGATAATTAGCCGATAAg              <  2:817694/149‑1 (MQ=255)
                                                                                                                             cgaTACGCTTCCGGGTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAg              <  1:766096/149‑1 (MQ=255)
                                                                                                                                    cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGc       <  2:258444/149‑1 (MQ=255)
                                                                                                                                      tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGa     <  2:291294/149‑1 (MQ=255)
                                                                                                                                        cGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGaca   <  1:552553/149‑1 (MQ=255)
                                                                                                                                         ggTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGacac  <  2:152646/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
AGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACAC  >  NC_000913/2019316‑2019601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑