Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,044,721 G→A 8.1% intergenic (+97/‑217) asnT → / → yeeJ tRNA‑Asn/putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,044,7210GA 8.1% 151.1 / 5.4 63intergenic (+97/‑217)asnT/yeeJtRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base A (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

GTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTAT  >  NC_000913/2044582‑2044869
                                                                                                                                           |                                                                                                                                                    
gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                             <  1:1000604/149‑1 (MQ=255)
 ttAATCCGTATGTCACTGGTTCGAGTCAAGTCAGATGAGCCAAAATCTAAAAAATCGCTTTTTTATCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATgg                                                                                                                                            <  1:251703/149‑1 (MQ=39)
   aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGTAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                          <  1:599119/149‑1 (MQ=255)
    aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                          <  2:510961/148‑1 (MQ=255)
    aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                          >  1:510961/1‑148 (MQ=255)
     tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGTGTTTTTAACATCTTAAGCGCCCTCGACATTTATGGTTGa                                                                                                                                        >  2:943525/1‑149 (MQ=255)
       cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCTATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                      <  2:851091/149‑1 (MQ=255)
       cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                      <  2:444660/149‑1 (MQ=255)
        gTATGGCACTGGTTAGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTATAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAggg                                                                                                                                     <  2:608372/149‑1 (MQ=38)
         tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGc                                                                                                                                    <  1:686208/149‑1 (MQ=39)
         tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGc                                                                                                                                    <  1:949127/149‑1 (MQ=39)
            gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGATTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGtt                                                                                                                                 >  1:503058/1‑149 (MQ=255)
               aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTg                                                                                                                              <  2:1004677/149‑1 (MQ=255)
                   gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                          >  2:765397/1‑149 (MQ=255)
                   gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTAAGCGCAATGTCACTCACCTTAGTTGAACATTGTTTTTTAACTGATAGAGGGTTTTTAACATCTTAATCGCCCTCGACATTTATGCTTGAGGGCGTTATGCTAt                                                                                                                          >  1:16351/1‑149 (MQ=255)
                       gAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGGTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAAc                                                                                                                      <  2:82074/149‑1 (MQ=255)
                            cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAAGATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAGCATTATGGTTGCGGGCGTTTTGCTATTAACGCCAt                                                                                                                 >  1:25847/1‑149 (MQ=255)
                            cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                 >  1:390299/1‑149 (MQ=255)
                            cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                 <  1:10975/149‑1 (MQ=255)
                              gTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCa                                                                                                               <  1:114165/149‑1 (MQ=255)
                                 agagGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCa                                                                                                            >  2:394611/1‑149 (MQ=255)
                                   aggagCCAAATTCTAAAAAATCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTTTTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAtt                                                                                                          <  1:271715/149‑1 (MQ=255)
                                      agCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTc                                                                                                       <  2:438117/149‑1 (MQ=255)
                                        ccAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                     >  1:809320/1‑149 (MQ=255)
                                          aaaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGATATGAACGCCATCACCATTTTCCCCt                                                                                                   >  1:105584/1‑149 (MQ=255)
                                           aaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTc                                                                                                  >  1:96633/1‑149 (MQ=255)
                                               cTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGagt                                                                                              >  2:821471/1‑147 (MQ=255)
                                                 gaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGTGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                            >  2:435783/2‑149 (MQ=255)
                                                 aaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                            <  2:378712/149‑1 (MQ=255)
                                                  aaaaTTCGCTTTTTTAGCGCAATGTCACGGCCCTTAGTTGAACATTGTTTTTTAACGGATAGAGGCTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGACGGCGTTTTGCTATGAACCCCATCACCATTTTCCCCTCGAAtata                                                                                           >  1:104800/1‑149 (MQ=255)
                                                   aaaTTCGCTTTTTTAGCGCAATGTCCCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCCTCACCATTTTCCCCTCGATTATaa                                                                                          >  1:252730/1‑149 (MQ=255)
                                                     aTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATaaaa                                                                                        <  1:468807/149‑1 (MQ=255)
                                                      ttCGCTTTTTTAGCGCAATGTCACTGCCCTTTGGTGTACATTTTTTTTTAACGGATAGGGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAGCACCATTTTCCCCTCGATTATAAAAc                                                                                       <  1:633733/149‑1 (MQ=255)
                                                           ttttttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                  >  2:9487/1‑149 (MQ=255)
                                                                tAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAtt                                                                             <  1:645924/149‑1 (MQ=255)
                                                                 aGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTc                                                                            <  1:532185/149‑1 (MQ=255)
                                                                     cAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCTACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagta                                                                        <  1:435206/149‑1 (MQ=255)
                                                                       aTGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagtagt                                                                      >  2:515266/1‑149 (MQ=255)
                                                                        tGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCTTCACCATTTTCCCCTCGATTATAAAACTTTAGTTATTCaggagtg                                                                     >  2:188123/1‑148 (MQ=255)
                                                                          tCACTGACCTTAGTTGAACATTTTTTTTTTACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAACTTTTTGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGtctc                                                                   <  2:581070/149‑1 (MQ=255)
                                                                            aCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                                                 <  1:646546/149‑1 (MQ=255)
                                                                               gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctc                                                              <  1:325204/149‑1 (MQ=255)
                                                                                       ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAAGAAAGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTAATTCAGTAGTCTCCCCTCTTCCAAca                                                      >  1:146861/1‑148 (MQ=255)
                                                                                         gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTAGCTATGAACGCCATCACCATTTTCACCTCGATTATAAAACTTGAGTTATTCTGTAGTCTCCCCTCTTGCAACTca                                                    >  2:235529/1‑149 (MQ=255)
                                                                                             aTTGTTTTTTAACGGATAGCGTGTTTTTAACATCTTAAGCGCCATCTACATTTATGGTTGAGGTCGTTTTGCTATGAACGCCATCACAATTTTACCCTCGATTATAAAACTTGAGTTAGTCAGTAGTCTCCCCTATTGCAACTcaaccc                                                >  2:988987/1‑145 (MQ=255)
                                                                                             aTTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAcc                                                >  2:919739/1‑149 (MQ=255)
                                                                                                      tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTTAGGGCGTTTTGCTATTAACGCCATCACCATTTTCCCCTCGATTAAAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                       >  1:160067/1‑149 (MQ=255)
                                                                                                      tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                       >  1:262163/1‑149 (MQ=255)
                                                                                                        aCGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCt                                     <  2:406182/149‑1 (MQ=255)
                                                                                                         aggATAGCGGGTTTTTAACATCTTAAGCTCCATCGACATTTATGGTTGAGGTCGTTTTGCTATGATCGCGATCACCATTTTCCACTCGATTATAATTGTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCGAATACTTCCTa                                    <  2:104800/148‑1 (MQ=255)
                                                                                                          ggATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTaa                                   >  1:774499/1‑149 (MQ=255)
                                                                                                           gATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAAc                                  <  2:390299/149‑1 (MQ=255)
                                                                                                                cGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGaaaa                             <  1:33538/149‑1 (MQ=255)
                                                                                                                   gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtt                          >  2:647905/1‑149 (MQ=255)
                                                                                                                   gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGGTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtt                          <  1:118493/149‑1 (MQ=255)
                                                                                                                       ttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatta                      >  1:42345/1‑149 (MQ=255)
                                                                                                                         aaCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAt                    >  2:415371/1‑149 (MQ=255)
                                                                                                                              catAAGCGCCCTCGCCCTATATGGGTGAGGGCGTTTTGCTATGAACGCCAACACCACTGTCCCCGCGAATATCAAACTCGAGATATTCAGTAGGCTCCCCTCTTCCACCTCACACCCACACCTCCCTAACGAAAAGTTATTAATTTTCa               <  2:62441/147‑1 (MQ=255)
                                                                                                                                      cccTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCAACGATTATAAAACTTGAGTTATTCAGTAGTAACCCCTCTTGCACCTCACACCCAAACCTGCCTAACGATTAGTTAATAATATTCAATCATAtt       >  1:54212/1‑149 (MQ=255)
                                                                                                                                      cccTCAACCATAAAGGTCGAGGGCGCTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATAtt       >  2:312900/1‑149 (MQ=255)
                                                                                                                                       ccTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTg      <  2:646833/149‑1 (MQ=255)
                                                                                                                                       ccTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTg      >  1:739422/1‑149 (MQ=255)
                                                                                                                                         tCGACCTTTATGGTTGAGGGCTTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCt    <  1:972466/149‑1 (MQ=255)
                                                                                                                                           gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAACACTGCCTAACGAAAAGTTATTAATTTTCAATGATATTGCTAt  <  1:968271/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
GTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTAT  >  NC_000913/2044582‑2044869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑