Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,044,729	T→A	7.6%	intergenic (+105/‑209)	asnT → / → yeeJ	tRNA‑Asn/putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,729	0	T	A	7.6%	171.1 / 4.6	66	intergenic (+105/‑209)	asnT/yeeJ	tRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base T (30/31);  new base A (2/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

GTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTT  >  NC_000913/2044582‑2044877                                                                                                                                                    |                                                                                                                                                     gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                                     <  1:1000604/149‑1 (MQ=255)  ttAATCCGTATGTCACTGGTTCGAGTCAAGTCAGATGAGCCAAAATCTAAAAAATCGCTTTTTTATCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATgg                                                                                                                                                    <  1:251703/149‑1 (MQ=39)    aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGTAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                                  <  1:599119/149‑1 (MQ=255)     aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                                  <  2:510961/148‑1 (MQ=255)     aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                                  >  1:510961/1‑148 (MQ=255)      tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGTGTTTTTAACATCTTAAGCGCCCTCGACATTTATGGTTGa                                                                                                                                                >  2:943525/1‑149 (MQ=255)        cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCTATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                              <  2:851091/149‑1 (MQ=255)        cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                              <  2:444660/149‑1 (MQ=255)         gTATGGCACTGGTTAGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTATAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAggg                                                                                                                                             <  2:608372/149‑1 (MQ=38)          tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGc                                                                                                                                            <  1:686208/149‑1 (MQ=39)          tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGc                                                                                                                                            <  1:949127/149‑1 (MQ=39)             gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGATTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGtt                                                                                                                                         >  1:503058/1‑149 (MQ=255)                aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTg                                                                                                                                      <  2:1004677/149‑1 (MQ=255)                    gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                                  >  2:765397/1‑149 (MQ=255)                    gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTAAGCGCAATGTCACTCACCTTAGTTGAACATTGTTTTTTAACTGATAGAGGGTTTTTAACATCTTAATCGCCCTCGACATTTATGCTTGAGGGCGTTATGCTAt                                                                                                                                  >  1:16351/1‑149 (MQ=255)                        gAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGGTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAAc                                                                                                                              <  2:82074/149‑1 (MQ=255)                             cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAAGATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAGCATTATGGTTGCGGGCGTTTTGCTATTAACGCCAt                                                                                                                         >  1:25847/1‑149 (MQ=255)                             cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                         >  1:390299/1‑149 (MQ=255)                             cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                         <  1:10975/149‑1 (MQ=255)                               gTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCa                                                                                                                       <  1:114165/149‑1 (MQ=255)                                  agagGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCa                                                                                                                    >  2:394611/1‑149 (MQ=255)                                    aggagCCAAATTCTAAAAAATCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTTTTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAtt                                                                                                                  <  1:271715/149‑1 (MQ=255)                                       agCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTc                                                                                                               <  2:438117/149‑1 (MQ=255)                                         ccAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                             >  1:809320/1‑149 (MQ=255)                                           aaaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGATATGAACGCCATCACCATTTTCCCCt                                                                                                           >  1:105584/1‑149 (MQ=255)                                            aaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTc                                                                                                          >  1:96633/1‑149 (MQ=255)                                                cTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGagt                                                                                                      >  2:821471/1‑147 (MQ=255)                                                  gaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGTGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                                    >  2:435783/2‑149 (MQ=255)                                                  aaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                                    <  2:378712/149‑1 (MQ=255)                                                   aaaaTTCGCTTTTTTAGCGCAATGTCACGGCCCTTAGTTGAACATTGTTTTTTAACGGATAGAGGCTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGACGGCGTTTTGCTATGAACCCCATCACCATTTTCCCCTCGAAtata                                                                                                   >  1:104800/1‑149 (MQ=255)                                                    aaaTTCGCTTTTTTAGCGCAATGTCCCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCCTCACCATTTTCCCCTCGATTATaa                                                                                                  >  1:252730/1‑149 (MQ=255)                                                      aTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATaaaa                                                                                                <  1:468807/149‑1 (MQ=255)                                                       ttCGCTTTTTTAGCGCAATGTCACTGCCCTTTGGTGTACATTTTTTTTTAACGGATAGGGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAGCACCATTTTCCCCTCGATTATAAAAc                                                                                               <  1:633733/149‑1 (MQ=255)                                                            ttttttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                          >  2:9487/1‑149 (MQ=255)                                                                 tAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAtt                                                                                     <  1:645924/149‑1 (MQ=255)                                                                  aGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTc                                                                                    <  1:532185/149‑1 (MQ=255)                                                                      cAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCTACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagta                                                                                <  1:435206/149‑1 (MQ=255)                                                                        aTGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagtagt                                                                              >  2:515266/1‑149 (MQ=255)                                                                         tGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCTTCACCATTTTCCCCTCGATTATAAAACTTTAGTTATTCaggagtg                                                                             >  2:188123/1‑148 (MQ=255)                                                                           tCACTGACCTTAGTTGAACATTTTTTTTTTACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAACTTTTTGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGtctc                                                                           <  2:581070/149‑1 (MQ=255)                                                                             aCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                                                         <  1:646546/149‑1 (MQ=255)                                                                                gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctc                                                                      <  1:325204/149‑1 (MQ=255)                                                                                        ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAAGAAAGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTAATTCAGTAGTCTCCCCTCTTCCAAca                                                              >  1:146861/1‑148 (MQ=255)                                                                                          gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTAGCTATGAACGCCATCACCATTTTCACCTCGATTATAAAACTTGAGTTATTCTGTAGTCTCCCCTCTTGCAACTca                                                            >  2:235529/1‑149 (MQ=255)                                                                                              aTTGTTTTTTAACGGATAGCGTGTTTTTAACATCTTAAGCGCCATCTACATTTATGGTTGAGGTCGTTTTGCTATGAACGCCATCACAATTTTACCCTCGATTATAAAACTTGAGTTAGTCAGTAGTCTCCCCTATTGCAACTcaaccc                                                        >  2:988987/1‑145 (MQ=255)                                                                                              aTTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAcc                                                        >  2:919739/1‑149 (MQ=255)                                                                                                       tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTTAGGGCGTTTTGCTATTAACGCCATCACCATTTTCCCCTCGATTAAAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                               >  1:160067/1‑149 (MQ=255)                                                                                                       tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                               >  1:262163/1‑149 (MQ=255)                                                                                                         aCGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCt                                             <  2:406182/149‑1 (MQ=255)                                                                                                          aggATAGCGGGTTTTTAACATCTTAAGCTCCATCGACATTTATGGTTGAGGTCGTTTTGCTATGATCGCGATCACCATTTTCCACTCGATTATAATTGTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCGAATACTTCCTa                                            <  2:104800/148‑1 (MQ=255)                                                                                                           ggATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTaa                                           >  1:774499/1‑149 (MQ=255)                                                                                                            gATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAAc                                          <  2:390299/149‑1 (MQ=255)                                                                                                                 cGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGaaaa                                     <  1:33538/149‑1 (MQ=255)                                                                                                                    gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtt                                  >  2:647905/1‑149 (MQ=255)                                                                                                                    gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGGTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtt                                  <  1:118493/149‑1 (MQ=255)                                                                                                                        ttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatta                              >  1:42345/1‑149 (MQ=255)                                                                                                                          aaCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAt                            >  2:415371/1‑149 (MQ=255)                                                                                                                               catAAGCGCCCTCGCCCTATATGGGTGAGGGCGTTTTGCTATGAACGCCAACACCACTGTCCCCGCGAATATCAAACTCGAGATATTCAGTAGGCTCCCCTCTTCCACCTCACACCCACACCTCCCTAACGAAAAGTTATTAATTTTCa                       <  2:62441/147‑1 (MQ=255)                                                                                                                                       cccTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCAACGATTATAAAACTTGAGTTATTCAGTAGTAACCCCTCTTGCACCTCACACCCAAACCTGCCTAACGATTAGTTAATAATATTCAATCATAtt               >  1:54212/1‑149 (MQ=255)                                                                                                                                       cccTCAACCATAAAGGTCGAGGGCGCTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATAtt               >  2:312900/1‑149 (MQ=255)                                                                                                                                        ccTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTg              <  2:646833/149‑1 (MQ=255)                                                                                                                                        ccTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTg              >  1:739422/1‑149 (MQ=255)                                                                                                                                          tCGACCTTTATGGTTGAGGGCTTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCt            <  1:972466/149‑1 (MQ=255)                                                                                                                                            gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAACACTGCCTAACGAAAAGTTATTAATTTTCAATGATATTGCTAt          <  1:968271/149‑1 (MQ=255)                                                                                                                                             aCCTTTATGGTTGAGGGCGTTTTGCTAAGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTAATTCAGTAGTCTCCCCTCTTGCAAATCCCACCCAAAACTGCCTAACGAAAAGTAATTAATTTTCAATCACATTCCAATc         >  2:989310/1‑149 (MQ=255)                                                                                                                                              ccTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCa        <  1:188123/149‑1 (MQ=255)                                                                                                                                                    tGGTTGAGGGCGTTTTGCTATGTACGCCATCACCATTTTCCCCTCGATTTTAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTAttt  <  2:146861/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTT  >  NC_000913/2044582‑2044877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑