Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,044,733 T→C 7.8% intergenic (+109/‑205) asnT → / → yeeJ tRNA‑Asn/putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,044,7330TC 7.8% 177.9 / 5.8 64intergenic (+109/‑205)asnT/yeeJtRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base C (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

AATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACAT  >  NC_000913/2044585‑2044881
                                                                                                                                                    |                                                                                                                                                    
aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGTAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                                      <  1:599119/149‑1 (MQ=255)
 aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                                      >  1:510961/1‑148 (MQ=255)
 aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt                                                                                                                                                      <  2:510961/148‑1 (MQ=255)
  tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGTGTTTTTAACATCTTAAGCGCCCTCGACATTTATGGTTGa                                                                                                                                                    >  2:943525/1‑149 (MQ=255)
    cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCTATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                                  <  2:851091/149‑1 (MQ=255)
    cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg                                                                                                                                                  <  2:444660/149‑1 (MQ=255)
     gTATGGCACTGGTTAGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTATAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAggg                                                                                                                                                 <  2:608372/149‑1 (MQ=38)
      tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGc                                                                                                                                                <  1:686208/149‑1 (MQ=39)
      tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCAACCATAAAGGTCGAGGGc                                                                                                                                                <  1:949127/149‑1 (MQ=39)
         gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGATTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGtt                                                                                                                                             >  1:503058/1‑149 (MQ=255)
            aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTg                                                                                                                                          <  2:1004677/149‑1 (MQ=255)
                gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAt                                                                                                                                      >  2:765397/1‑149 (MQ=255)
                gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTAAGCGCAATGTCACTCACCTTAGTTGAACATTGTTTTTTAACTGATAGAGGGTTTTTAACATCTTAATCGCCCTCGACATTTATGCTTGAGGGCGTTATGCTAt                                                                                                                                      >  1:16351/1‑149 (MQ=255)
                    gAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGGTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAAc                                                                                                                                  <  2:82074/149‑1 (MQ=255)
                         cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAAGATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAGCATTATGGTTGCGGGCGTTTTGCTATTAACGCCAt                                                                                                                             >  1:25847/1‑149 (MQ=255)
                         cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                             >  1:390299/1‑149 (MQ=255)
                         cAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAt                                                                                                                             <  1:10975/149‑1 (MQ=255)
                           gTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCa                                                                                                                           <  1:114165/149‑1 (MQ=255)
                              agagGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCa                                                                                                                        >  2:394611/1‑149 (MQ=255)
                                aggagCCAAATTCTAAAAAATCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTTTTTTTTAACGGATAGCGGGGTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAtt                                                                                                                      <  1:271715/149‑1 (MQ=255)
                                   agCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTc                                                                                                                   <  2:438117/149‑1 (MQ=255)
                                     ccAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTccc                                                                                                                 >  1:809320/1‑149 (MQ=255)
                                       aaaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGATATGAACGCCATCACCATTTTCCCCt                                                                                                               >  1:105584/1‑149 (MQ=255)
                                        aaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTc                                                                                                              >  1:96633/1‑149 (MQ=255)
                                            cTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGagt                                                                                                          >  2:821471/1‑147 (MQ=255)
                                              gaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGTGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                                        >  2:435783/2‑149 (MQ=255)
                                              aaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATtat                                                                                                        <  2:378712/149‑1 (MQ=255)
                                               aaaaTTCGCTTTTTTAGCGCAATGTCACGGCCCTTAGTTGAACATTGTTTTTTAACGGATAGAGGCTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGACGGCGTTTTGCTATGAACCCCATCACCATTTTCCCCTCGAAtata                                                                                                       >  1:104800/1‑149 (MQ=255)
                                                aaaTTCGCTTTTTTAGCGCAATGTCCCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCCTCACCATTTTCCCCTCGATTATaa                                                                                                      >  1:252730/1‑149 (MQ=255)
                                                  aTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATaaaa                                                                                                    <  1:468807/149‑1 (MQ=255)
                                                   ttCGCTTTTTTAGCGCAATGTCACTGCCCTTTGGTGTACATTTTTTTTTAACGGATAGGGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCAGCACCATTTTCCCCTCGATTATAAAAc                                                                                                   <  1:633733/149‑1 (MQ=255)
                                                        ttttttAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAg                                                                                              >  2:9487/1‑149 (MQ=255)
                                                             tAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTAtt                                                                                         <  1:645924/149‑1 (MQ=255)
                                                              aGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTc                                                                                        <  1:532185/149‑1 (MQ=255)
                                                                  cAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCTACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagta                                                                                    <  1:435206/149‑1 (MQ=255)
                                                                    aTGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCagtagt                                                                                  >  2:515266/1‑149 (MQ=255)
                                                                     tGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCTTCACCATTTTCCCCTCGATTATAAAACTTTAGTTATTCaggagtg                                                                                 >  2:188123/1‑148 (MQ=255)
                                                                       tCACTGACCTTAGTTGAACATTTTTTTTTTACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAACTTTTTGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGtctc                                                                               <  2:581070/149‑1 (MQ=255)
                                                                         aCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                                                             <  1:646546/149‑1 (MQ=255)
                                                                            gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctc                                                                          <  1:325204/149‑1 (MQ=255)
                                                                                    ttGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTAAGAAAGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTAATTCAGTAGTCTCCCCTCTTCCAAca                                                                  >  1:146861/1‑148 (MQ=255)
                                                                                      gAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTAGCTATGAACGCCATCACCATTTTCACCTCGATTATAAAACTTGAGTTATTCTGTAGTCTCCCCTCTTGCAACTca                                                                >  2:235529/1‑149 (MQ=255)
                                                                                          aTTGTTTTTTAACGGATAGCGTGTTTTTAACATCTTAAGCGCCATCTACATTTATGGTTGAGGTCGTTTTGCTATGAACGCCATCACAATTTTACCCTCGATTATAAAACTTGAGTTAGTCAGTAGTCTCCCCTATTGCAACTcaaccc                                                            >  2:988987/1‑145 (MQ=255)
                                                                                          aTTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAcc                                                            >  2:919739/1‑149 (MQ=255)
                                                                                                   tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTTAGGGCGTTTTGCTATTAACGCCATCACCATTTTCCCCTCGATTAAAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                                   >  1:160067/1‑149 (MQ=255)
                                                                                                   tAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGc                                                   >  1:262163/1‑149 (MQ=255)
                                                                                                     aCGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCt                                                 <  2:406182/149‑1 (MQ=255)
                                                                                                      aggATAGCGGGTTTTTAACATCTTAAGCTCCATCGACATTTATGGTTGAGGTCGTTTTGCTATGATCGCGATCACCATTTTCCACTCGATTATAATTGTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCGAATACTTCCTa                                                <  2:104800/148‑1 (MQ=255)
                                                                                                       ggATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTaa                                               >  1:774499/1‑149 (MQ=255)
                                                                                                        gATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAAc                                              <  2:390299/149‑1 (MQ=255)
                                                                                                             cGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGaaaa                                         <  1:33538/149‑1 (MQ=255)
                                                                                                                gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtt                                      >  2:647905/1‑149 (MQ=255)
                                                                                                                gTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGGTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGtt                                      <  1:118493/149‑1 (MQ=255)
                                                                                                                    ttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatta                                  >  1:42345/1‑149 (MQ=255)
                                                                                                                      aaCATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAAt                                >  2:415371/1‑149 (MQ=255)
                                                                                                                           catAAGCGCCCTCGCCCTATATGGGTGAGGGCGTTTTGCTATGAACGCCAACACCACTGTCCCCGCGAATATCAAACTCGAGATATTCAGTAGGCTCCCCTCTTCCACCTCACACCCACACCTCCCTAACGAAAAGTTATTAATTTTCa                           <  2:62441/147‑1 (MQ=255)
                                                                                                                                   cccTCAACCATAAAGGTCGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCAACGATTATAAAACTTGAGTTATTCAGTAGTAACCCCTCTTGCACCTCACACCCAAACCTGCCTAACGATTAGTTAATAATATTCAATCATAtt                   >  1:54212/1‑149 (MQ=255)
                                                                                                                                   cccTCAACCATAAAGGTCGAGGGCGCTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATAtt                   >  2:312900/1‑149 (MQ=255)
                                                                                                                                    ccTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTg                  >  1:739422/1‑149 (MQ=255)
                                                                                                                                    ccTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTg                  <  2:646833/149‑1 (MQ=255)
                                                                                                                                      tCGACCTTTATGGTTGAGGGCTTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCt                <  1:972466/149‑1 (MQ=255)
                                                                                                                                        gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAACACTGCCTAACGAAAAGTTATTAATTTTCAATGATATTGCTAt              <  1:968271/149‑1 (MQ=255)
                                                                                                                                         aCCTTTATGGTTGAGGGCGTTTTGCTAAGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTAATTCAGTAGTCTCCCCTCTTGCAAATCCCACCCAAAACTGCCTAACGAAAAGTAATTAATTTTCAATCACATTCCAATc             >  2:989310/1‑149 (MQ=255)
                                                                                                                                          ccTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCa            <  1:188123/149‑1 (MQ=255)
                                                                                                                                                tGGTTGAGGGCGTTTTGCTATGTACGCCATCACCATTTTCCCCTCGATTTTAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTAttt      <  2:146861/149‑1 (MQ=255)
                                                                                                                                                 ggTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTa     <  1:943525/149‑1 (MQ=255)
                                                                                                                                                  gTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCGCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCACCTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATTGTGATATCAGTATTTAc    >  2:474575/1‑149 (MQ=255)
                                                                                                                                                    tGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACAt  <  2:252730/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACAT  >  NC_000913/2044585‑2044881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑