Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,231,175 G→A 8.1% D53N (GAC→AAC)  yohK → LrgB family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,231,1750GA 8.1% 125.9 / 3.2 50D53N (GAC→AAC) yohKLrgB family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (17/28);  new base A (2/2);  total (19/31)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

TATCTGGTGGTCATTACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGC  >  NC_000913/2231030‑2231312
                                                                                                                                                 |                                                                                                                                         
tatCTGGTGGTCATTCCCGCTGACATTGATTGTCTTTTTTGCCGCCCCCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGAGCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACa                                                                                                                                        <  1:760135/149‑1 (MQ=255)
    tggtggTCATTACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGGTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTa                                                                                                                                    <  1:304833/149‑1 (MQ=255)
     ggtggtCATTACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCTTTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTAc                                                                                                                                   <  2:918107/149‑1 (MQ=255)
         gtCATTACCGCTGACAATGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTa                                                                                                                               <  1:785857/149‑1 (MQ=255)
          tgaTTACCGCTGACATTGATTTTCTTTTTTGCCGCCCTCATACTGGCGGCACGGTATAAGTTTGCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTaa                                                                                                                              <  2:662174/147‑1 (MQ=255)
          tCATTACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACAACAGCTACTTTaa                                                                                                                              >  2:528199/1‑149 (MQ=255)
          tCATTACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACAACAGCTACTTTaa                                                                                                                              >  2:662587/1‑149 (MQ=255)
          tCATAACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTaa                                                                                                                              <  2:525910/149‑1 (MQ=255)
           cATTACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTaaa                                                                                                                             <  1:62116/149‑1 (MQ=255)
                    tGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGaa                                                                                                                    >  1:156552/1‑149 (MQ=255)
                             ttgTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAAc                                                                                                           >  1:723857/1‑149 (MQ=255)
                              tgTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACg                                                                                                          <  2:27112/149‑1 (MQ=255)
                                  ttttttGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATct                                                                                                      >  1:488326/1‑149 (MQ=255)
                                    ttttGCCGCCCGCAAACTGGCTGCACGGTCTAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATctgc                                                                                                    <  2:600393/149‑1 (MQ=255)
                                     tttGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATctgct                                                                                                   <  2:573108/149‑1 (MQ=255)
                                          cgcccgcAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAAc                                                                                              <  1:702699/149‑1 (MQ=255)
                                            cccgcAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCACCcg                                                                                            >  1:22731/1‑149 (MQ=255)
                                              cgcAAACTGGCGGCACGGTATAAGGTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGCTCAGTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGGGCTAAACGATCTGCTGCAACcggc                                                                                          >  1:727250/1‑149 (MQ=255)
                                               gcAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACcggcg                                                                                         <  2:406436/149‑1 (MQ=255)
                                                  aaCTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGgtc                                                                                      <  1:23237/149‑1 (MQ=255)
                                                     tGGCGGCACTGTATAAGATTCCCTTGCATAACCCGTTGCTGGTAGCAACGGGGGTGATCCTTCCTTTTTTAATGCCGACTGGCATCTCTTCCGCCAGCTACTTAAACGGTAGCGAAGGGCTAAACGATCTGCTGCAACCGGCGgtcgtc                                                                                   <  2:56684/149‑1 (MQ=255)
                                                       gcggcACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGAAACCGGCGGTCGTcgc                                                                                 >  2:908255/1‑149 (MQ=255)
                                                         ggcACGGTATAAGTTTCAGTTGCTTAAGCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTAAGAAAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGGCGTCgcgc                                                                               <  2:975014/149‑1 (MQ=255)
                                                         ggcACGGTATAAGTTTACCTTGCTTAACCCGTTGCTGGTATCAATGGTGGTGATCATTCCTTTATAAATGCTGACAGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTACACGATCAGCTGCAACCGTCGTTCTTCgagc                                                                               >  2:998592/1‑149 (MQ=255)
                                                           cACGGTATAAGATTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTTGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGAAACCTGCGTTCGTCGCGCTg                                                                             >  2:733900/1‑149 (MQ=255)
                                                            aCGGTATAAGTGTCGCTTGCTTAACCCGTAGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCAGCTCTTACGACCGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTgg                                                                            <  2:283060/149‑1 (MQ=255)
                                                             cGGTTTAAGTTTCGCTTGCTTAACCGGTTGCTGGTAGCAATGGTGGTTATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGTCAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGc                                                                           <  2:144328/149‑1 (MQ=255)
                                                             cGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTACTGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGGCGCGCTGGc                                                                           <  2:55894/149‑1 (MQ=255)
                                                             cGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGc                                                                           <  2:121660/149‑1 (MQ=255)
                                                                tataAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTa                                                                        >  2:1005777/1‑149 (MQ=255)
                                                                   aaGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTAGCGCTGGCCTATcc                                                                     >  1:890115/1‑149 (MQ=255)
                                                                    aGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGAAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCt                                                                    >  1:395424/1‑149 (MQ=255)
                                                                        tCCCTTTCTTAACCCGTTGCTGGTAGAAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCTTCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCTACCGGCGGTCGGCGCGCTGGCCTATCCTTtat                                                                <  1:711470/149‑1 (MQ=255)
                                                                        tCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTtat                                                                <  2:377195/149‑1 (MQ=255)
                                                                           cTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACAACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATg                                                             <  1:528199/149‑1 (MQ=255)
                                                                           cTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACAACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATg                                                             <  1:662587/149‑1 (MQ=255)
                                                                                 tAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGagcagc                                                       <  2:331522/149‑1 (MQ=255)
                                                                                    cccGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTac                                                    <  1:262779/149‑1 (MQ=255)
                                                                                              gTAGCAATGGTGTTGATCATTCCTTTTTTAATGCTGACTGGCCTCTCTTACGACAGCTACTATAAAGGTAACGAAGAGCTAAACGATCTGCTTCAACCGGCGGTCTTCGCGCTGGCCTATCCTTTATATGAGCAGCTAGACCaaagacg                                          >  1:703551/1‑143 (MQ=255)
                                                                                                 ctaaTGTTGGTGTTCATTAATTTATTACTGCTGACTGGCATCTATGACTAAAGATATTTTCAAGGTATCGAAGTGCTAAACGACCGGCTGCAAACGGCTTTCGGCGCGCTGGCCTATCCTTGATATGAGCAGCTACACCAGATCcgcgc                                       <  1:676183/147‑1 (MQ=255)
                                                                                                   aaTGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAATGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCcgcgcgc                                     >  1:270062/1‑149 (MQ=255)
                                                                                                     tggtggTGATCATTCCTTTTTTAATGCTGACTTGCATGTATTTCGACAGCTAATTTAAAGTTATCGCAGTGCTAATCGATCAGCTGCAATCGGCGGGCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCgcgcgcgc                                   <  2:417836/149‑1 (MQ=255)
                                                                                                     tggtggTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCgcgcgcgc                                   >  1:159583/1‑149 (MQ=255)
                                                                                                       gtggtgATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCGCGCGCGCTg                                 <  1:987328/149‑1 (MQ=255)
                                                                                                               cATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGa                         <  2:22731/149‑1 (MQ=255)
                                                                                                                    cTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGATCAGCTACACCAGATCCGCGCGCGCTGGAAATCGatcatc                    >  1:171298/1‑149 (MQ=255)
                                                                                                                           aaTGCAGACGGGCATCTCATACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCGCGCGCGCGGGAAATCGATCATCACCATCt             <  1:315427/149‑1 (MQ=255)
                                                                                                                              gCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTCCACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGtt          >  1:307939/1‑149 (MQ=255)
                                                                                                                                   cTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATc     <  2:457800/149‑1 (MQ=255)
                                                                                                                                      gCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCACCCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGc  >  1:902597/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
TATCTGGTGGTCATTACCGCTGACATTGATTGTCTTTTTTGCCGCCCGCAAACTGGCGGCACGGTATAAGTTTCCCTTGCTTAACCCGTTGCTGGTAGCAATGGTGGTGATCATTCCTTTTTTAATGCTGACTGGCATCTCTTACGACAGCTACTTTAAAGGTAGCGAAGTGCTAAACGATCTGCTGCAACCGGCGGTCGTCGCGCTGGCCTATCCTTTATATGAGCAGCTACACCAGATCCGCGCGCGCTGGAAATCGATCATCACCATCTGTTTTATCGGC  >  NC_000913/2231030‑2231312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑