Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,432,985	A→G	5.9%	intergenic (‑43/+27)	folC ← / ← accD	bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,432,985	0	A	G	5.9%	162.6 / 3.0	68	intergenic (‑43/+27)	folC/accD	bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
Reads supporting (aligned to +/- strand):  ref base A (37/26);  new base G (2/2);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

GCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCG  >  NC_000913/2432841‑2433128                                                                                                                                                 |                                                                                                                                                gcgcTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCAAGAGGCGCCGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCa                                                                                                                                             >  1:1016560/1‑149 (MQ=255)  cgcTCACGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAg                                                                                                                                            <  1:895539/149‑1 (MQ=255)     tCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTgg                                                                                                                                         >  1:790967/1‑149 (MQ=255)        aGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccc                                                                                                                                      >  2:629195/1‑149 (MQ=255)        aGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGGTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccc                                                                                                                                      >  1:833631/1‑149 (MQ=255)         ggCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccct                                                                                                                                     >  2:489382/1‑149 (MQ=255)          gCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctg                                                                                                                                    <  2:22482/149‑1 (MQ=255)           ccGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTTCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGgctctgc                                                                                                                                   <  1:570508/149‑1 (MQ=255)            cGAGATCGATAGTTTTAATGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctgcc                                                                                                                                  <  1:572904/149‑1 (MQ=255)                aTCGATAGTTTTCCTGTGCAGGTGTTCCAGATAAGAAAGCCACTAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGCAATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCtta                                                                                                                              <  2:147434/149‑1 (MQ=255)                 tCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAACGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTTAGAATAGCAAAAGGGCAGGGCCACTGGCTCTGCCCtttt                                                                                                                             <  1:541042/149‑3 (MQ=255)                  cGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGAGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatc                                                                                                                            >  2:821297/1‑149 (MQ=255)                   gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCTCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                           >  1:910076/1‑149 (MQ=255)                   gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                           >  1:617059/1‑149 (MQ=255)                    aTAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGTCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGTCCCTGCCGttatcag                                                                                                                          >  1:691912/1‑149 (MQ=255)                    aTAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcat                                                                                                                          >  1:216120/1‑148 (MQ=255)                    aTAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcag                                                                                                                          >  1:393299/1‑149 (MQ=255)                         tttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatc                                                                                                                     <  2:617059/149‑1 (MQ=255)                          ttAATGAGCAGGTTTTCCACTTAAGAAAGCCACCAAGGCAGAGGCGAAGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGATACGGTGATAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatca                                                                                                                    <  1:584424/149‑1 (MQ=255)                              tgtgCATGTTTTCCAGATAAGAAAGCCACGAAGCCAGATGCGACGCGCCTAGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTATTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGcc                                                                                                                >  2:254325/1‑149 (MQ=255)                                      ttttCCAGATATGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGGGCGTTTGATAATCATGGTAGCCGCTGATTCGTTACGGTGAGAATAGCAAAAGCGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCCGGTTc                                                                                                        <  1:691276/149‑1 (MQ=255)                                        ttCCAGATAAGAAAGCCACGAAGCCAGAGGCGCCGCGGCTCGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCt                                                                                                      >  1:590930/1‑149 (MQ=255)                                             gATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGTCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATcc                                                                                                 >  2:185846/1‑149 (MQ=255)                                             gATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATcc                                                                                                 >  2:182490/1‑149 (MQ=255)                                                aaGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCATGCCTCAGGTTCCTGATCCGGt                                                                                              >  2:298742/1‑149 (MQ=255)                                                  gAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCCTGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCAGATCAGATAGCCGGCCTCAGGTTCCTGATCCGGTAc                                                                                            >  1:557900/1‑149 (MQ=255)                                                  gAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTAc                                                                                            >  1:109988/1‑149 (MQ=255)                                                       ccACGACGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgggg                                                                                       >  1:135367/1‑149 (MQ=255)                                                         ccGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTCCGGAGAGAATAGCAACAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGt                                                                                     <  1:568333/148‑1 (MQ=255)                                                           gAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTAc                                                                                   <  1:555181/149‑1 (MQ=255)                                                             aGCCAGAGGCGACGCGGCGTGAGGAGTGCGTTAGATAATCATGGTATCCGCTGATTCCTTACGGTGAGAATAGCAAAAGGGCAGCGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGATCCTGATCCGGTACCGGGGGTACCa                                                                                 <  2:286359/149‑1 (MQ=255)                                                              gCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCAc                                                                                >  1:460774/1‑149 (MQ=255)                                                                    ggCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGcc                                                                          >  2:123514/1‑149 (MQ=255)                                                                     gCGACGCGGCTTGAGTAGTGCGTTTGATAATCATGGTATCCGAAGATTAGTTACGGTGAGAATAGCAAAAGGGCAGAGACAGTGGCCCTGACCTTAACAGTTATCAGGCCTCATGTTCCTGATCCGGTACCGGGGGTACCACTACGCCt                                                                         >  1:110515/1‑149 (MQ=255)                                                                     gCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGCGGGTACCACTACGCCt                                                                         >  2:782827/1‑149 (MQ=255)                                                                     ccGACGCGGCTTGAGGAGAGCGTTTGATAATCAAGGTATCCGCTGATTCGTTACGGAGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCt                                                                         <  1:432171/148‑1 (MQ=255)                                                                                  aggagTGCGTTTGATAATCATGGTTTCAGCTGATTCGTTACGGTGAGAAAAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCtt                                                            <  1:900163/149‑1 (MQ=255)                                                                                    gagTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCa                                                          >  2:596889/1‑149 (MQ=255)                                                                                      gTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGGGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAgg                                                        <  1:424395/149‑1 (MQ=255)                                                                                       tGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGa                                                       >  2:382731/1‑149 (MQ=255)                                                                                                 aaTCCTGGTATCCGCTGATTCGTTACGGGGAGAATAGCAAACGGGCCGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTGCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTg                                             <  1:1002757/149‑1 (MQ=255)                                                                                                  aTCATGGTATCCGCTGATTCGTTACGGTGCGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCCGGTTCCTGTTCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTgg                                            <  1:489382/149‑1 (MQ=255)                                                                                                   tCATGGGAGCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGc                                           <  1:852260/149‑1 (MQ=255)                                                                                                    cATGGTATCTGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCa                                          <  2:370847/149‑1 (MQ=255)                                                                                                     aTGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAg                                         >  2:771941/1‑149 (MQ=255)                                                                                                      tGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGa                                        >  2:801921/1‑149 (MQ=255)                                                                                                       ggTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCGCAGGTTCCTGATCCGGGACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGAt                                       <  2:1019138/149‑1 (MQ=255)                                                                                                        gTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGAtt                                      >  2:915385/1‑149 (MQ=255)                                                                                                             cGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcatca                                 >  1:961695/1‑149 (MQ=255)                                                                                                                      gTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTACGCCa                        >  2:69825/1‑149 (MQ=255)                                                                                                                          cGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGCTCCGGTACCGGGGGTACCACTACGCCTTCACGCCGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGaaa                    >  1:936379/1‑148 (MQ=255)                                                                                                                               agaATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTTCAACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGCTTCATCCACTTCGCCAGCATGCTCg               >  2:922476/1‑149 (MQ=255)                                                                                                                               agaATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCg               >  1:293170/1‑149 (MQ=255)                                                                                                                                taaTAGCAAAAGGGCAGAGCCAGTGGCCGTGCACTTATCAGTTATCAGGCCTCAGGGTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGc              <  2:990193/148‑1 (MQ=255)                                                                                                                                 aaTAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGcc             >  1:303275/1‑149 (MQ=255)                                                                                                                                   tAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAg           <  2:109988/149‑1 (MQ=255)                                                                                                                                     gCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGGGCTTCAGGATTCGGCGCTGGCCGCTTCATCAACTTCGCCAGACTGCTCGCCAGtt         >  1:123988/1‑149 (MQ=255)                                                                                                                                       aaaaGGTCAGATCAATTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGTTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACTCTGCGATTCAGGATTCGTCGCTGGCATATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc       <  2:188097/149‑1 (MQ=255)                                                                                                                                       aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc       <  1:407877/149‑1 (MQ=255)                                                                                                                                       aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc       <  1:483373/149‑1 (MQ=255)                                                                                                                                         aaGGGCTGAGCCAGTGGCCCTGCCATTATCAGTTATCCGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg     <  2:961695/149‑1 (MQ=255)                                                                                                                                         aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg     <  1:262182/149‑1 (MQ=255)                                                                                                                                         aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg     <  1:802171/149‑1 (MQ=255)                                                                                                                                          agggcagggcCACTGGCTCTGCCCTTATCAGTTATCAGGCCTCAGGTTGCTGAGCCGGTACCGGGGGTTCCACTACGCCTTCAAGCGGCGCGGAAGGATTCGGCGCTGGCAGATTCATCAACATCGCCCGAATGATCACAAGATTCAgg    >  1:422588/1‑149 (MQ=255)                                                                                                                                           gggCAGGGCCACTGGCTCTGCCCTGATAAGTTATCAGGCCTAAGGTTCCTGATCCGGTACCGGGGGTACCACAAAGCCTTCACGCGCCGCTTCAGCATTCGGCGCTGGCTGCTTCATCCACTTCGCCCGAAAGCTCGCCAGTTTCCGgc   >  2:127326/1‑149 (MQ=255)                                                                                                                                           gggCAGAGCCAGT‑GCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcg  >  1:316470/1‑149 (MQ=255)                                                                                                                                            ggCAGGGCCACTGGCTCTGCCCTTTTCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcg  >  1:545123/1‑149 (MQ=255)                                                                                                                                             gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg     >  2:250850/1‑145 (MQ=255)                                                                                                                                             gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg     <  1:250850/145‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                GCGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCG  >  NC_000913/2432841‑2433128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑