Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,228 G→C 10.1% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2280GC10.1% 1201.5 / 22.3 386intergenic (‑189/+179)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base G (130/209);  new base C (40/4);  total (173/213)
Fisher's exact test for biased strand distribution p-value = 9.91e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 397 total aligned reads displayed.

TCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351080‑1351377
                                                                                                                                                    |                                                                                                                                                      
tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                       <  2:2886826/150‑1 (MQ=255)
        ttCGTCTTTTTAGTAAAGAGTACGAACAGATAAACGGTTTTTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCAGACCGGATCATGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTTACCCTCTCCCCAGAGGGGCgtg                                                                                                                                               <  1:4375413/150‑3 (MQ=255)
         tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCTGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                              <  1:2912102/150‑1 (MQ=255)
         tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                              <  2:6584297/150‑1 (MQ=255)
             cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAAACCACACCCCACAGGGGCGAGGGGAc                                                                                                                                          >  1:4641254/1‑150 (MQ=255)
               ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg                                                                                                                                        >  2:9130356/1‑150 (MQ=255)
               ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCGAACCCACACCCCAGAGGGGCGAGGGGCCCg                                                                                                                                        >  2:1935461/1‑150 (MQ=255)
                    gTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCCCCCCAACCCACACCCCAGAGGGGCGAGGGGACCGTATtg                                                                                                                                   >  1:355031/1‑150 (MQ=255)
                       aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGGATAGTGc                                                                                                                                >  2:8903031/1‑150 (MQ=255)
                          aGTACGAACAGATAACCGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                             <  1:5885138/150‑1 (MQ=255)
                          aGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                             <  1:1368177/150‑1 (MQ=255)
                              cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                        <  2:565334/150‑1 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                       <  1:8307356/150‑1 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCGAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAAAATTg                                                                                                                       >  2:3621454/1‑150 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCACACCCCACAGGGGCGCGGGGACCCTATTGTGGAAATATTg                                                                                                                       >  1:3415118/1‑150 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                       >  2:5522293/1‑150 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                                                                      <  2:1399691/150‑1 (MQ=255)
                                aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTCACCCGAACCCTCACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                                                                      >  2:3089843/1‑150 (MQ=255)
                                 aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGtt                                                                                                                     <  2:4325685/150‑1 (MQ=255)
                                 aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCAAACCCAAAACCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGAAAATATtttt                                                                                                                     >  2:2032448/1‑150 (MQ=255)
                                  cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTCACCCAAACACACAACCCCCAGGGGCGAGGGGACCCGAATGTGCAAATATAGTTa                                                                                                                    >  1:5772730/1‑150 (MQ=255)
                                   aGATAACCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                                                                                   <  2:4828013/150‑1 (MQ=255)
                                     gtAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTAGAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCCCACCCCAACACACACCCCAGAGGGGCGCGGGGCCCGGATTGGGGCAAGATTGTTTccc                                                                                                                 >  1:4111164/2‑150 (MQ=255)
                                      tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcccc                                                                                                                <  2:797396/150‑1 (MQ=255)
                                       aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCa                                                                                                               <  1:8675792/150‑1 (MQ=255)
                                       aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCa                                                                                                               <  1:8012308/150‑1 (MQ=255)
                                       aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCCAACCCACAACCCACAGGGGCGAGGGGCCCGTATTGTTGAAATATtttttaaccc                                                                                                               >  1:2601464/1‑144 (MQ=255)
                                            gTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAAACCACACCCCACAGGGGCGCGGGGGCCGGATTTTGGAAAAAATTTTACACCACcaac                                                                                                          >  1:653088/1‑150 (MQ=255)
                                               attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                       <  1:399569/150‑1 (MQ=255)
                                               attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCCCACCCAAAACCCCACCCCCGAGGGGCGCGGGGGCCGGATTGTGCCAAAATTGTTTACCCCCCaacaaa                                                                                                       >  2:8544693/1‑150 (MQ=255)
                                                ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaac                                                                                                      >  1:9015498/1‑150 (MQ=255)
                                                 tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCATATTGTGCAAATATTGTTACCCCAGCaacaaaca                                                                                                     <  2:7851934/150‑1 (MQ=255)
                                                 tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCAGAGGGGCGAGGGGACCGGAGTGTGCAAATATTGTTACCCCAGCaacaaaca                                                                                                     >  2:5920892/1‑150 (MQ=255)
                                                   taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                   <  1:1059825/150‑1 (MQ=255)
                                                   taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                   <  1:8822146/150‑1 (MQ=255)
                                                    aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc                                                                                                  >  2:2174848/1‑150 (MQ=255)
                                                    aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCAGACCCAAAACCAAAACCCAGAGGGGCGGGGGGGCCGTTTTTTGGAAAAATTTTTTACCCAGCCACAAAAAGGc                                                                                                  >  1:7521901/1‑150 (MQ=255)
                                                     aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGGTTGCCCGCCCCCAAACCCACACCCCCCCGGGGCGGGGGGGCCGGCTTGTGGAAATATTTTTTACCCACCAACAAAAAGGcg                                                                                                 >  1:4294983/1‑149 (MQ=255)
                                                      tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                                <  1:5272840/150‑1 (MQ=255)
                                                          ccTGGCTGAGAGTAGCTATAGTTGCCAGGTCCCACCGGAGCAGGCTGCGGCAGGGGGGGCGCAATTCCCCTCACCCTAACCCACTCCCCACAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCACCAACAAACAGGCACATAc                                                                                            <  2:4389759/150‑1 (MQ=255)
                                                            tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAg                                                                                          <  1:2670783/150‑1 (MQ=255)
                                                            tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTCACCCGAACCCACACCCCCGAGGGGCGCGGGGACCGTATTGTGCAAATAATGTTAACCCACCAACAAAAAGGCGCATACAg                                                                                          >  2:2525321/1‑150 (MQ=255)
                                                                 gtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCCAAACCACACCCCACAGGGGCGAGGGGGCCCGAGTGTGCAAAAATTTTTACCCCCACAACAAAAACGCGCATACACccccc                                                                                     >  1:6169973/1‑149 (MQ=255)
                                                                   gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                   <  1:4878690/150‑1 (MQ=255)
                                                                     ggaGCGATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  2:9019071/148‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCACACCCCAAACCACACCCCCCACGGGGGAGGGGGCCCGAATGTGGAAAAATTTTTACCCCACCAACAAAAAAGCGCAGACAGCCCCCAAcc                                                                                 >  1:7206201/1‑150 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  1:7635251/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  2:640923/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  2:7515055/150‑1 (MQ=255)
                                                                       aGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCCCACCCCAACCCCCAACCCACAGGGGGGCGGGGGCCGGCTTTTGGAAATAATTTTTAACCCGCAACCAACACGCGCAAACAGCCCCAAAaccg                                                                               >  1:705288/1‑149 (MQ=255)
                                                                          tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCAGAGGGGCGAGGGGCCCGTAATTTGCAAATATTGTTACCCCCGCCACAAACAGGCTCATACCGCCCCTAAcccctgc                                                                            >  2:413933/1‑146 (MQ=38)
                                                                          tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCGAACCCGCACCCCAGAGGGGCGAGGGGCCCCGATTGTGCAAATATTGTTACCCCACCAACAAACAGGCGCATACAGCCCCGAACCCATTc                                                                            >  2:862808/1‑150 (MQ=255)
                                                                           ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCTCCCCAGAGGGGCGAGGGGACCGTAATGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                                                                           >  2:8432546/1‑150 (MQ=255)
                                                                            taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCAt                                                                          >  2:5402064/1‑150 (MQ=255)
                                                                            taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTGTTCCCCACCACCGAAACCGCACCCCCAACGGGCGAGGGGACCGGATTGTGCAAAAAATTTTAACCCCCCACAAAACAGGCGAAGTCAGCCCCTAACCCAGTCAt                                                                          >  2:1749950/1‑150 (MQ=255)
                                                                              gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                        <  1:5327616/150‑1 (MQ=255)
                                                                               ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc                                                                       <  2:7643619/150‑1 (MQ=255)
                                                                               ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc                                                                       <  1:6799122/150‑1 (MQ=255)
                                                                               ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCGAAACCACACCCCAGAGGGGCGAGGGGACCGTAATTTGCCAATATTTTTACCCCAGCAACAAACAGGCGCATACCCCCCCAAACCCATTCATGGc                                                                       >  2:3427797/1‑150 (MQ=255)
                                                                                 gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGa                                                                     <  1:7330284/150‑1 (MQ=255)
                                                                                      gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCt                                                                <  2:6854922/150‑1 (MQ=255)
                                                                                         cGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTGCCCCAGCAACAAACAGGCTCAAACAGCCCCGAACCCTTTCATGGCGATGGCTggg                                                             >  2:8371396/1‑150 (MQ=255)
                                                                                          gACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa                                                            <  1:1129458/150‑1 (MQ=255)
                                                                                           aCCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGACCCTAACCCTTTCATGGCGATGGCTGGGAc                                                           >  2:4921522/1‑150 (MQ=255)
                                                                                           aCCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAAACCACACCCCACAGGGGCGAGGGGGCCCTAATGTTCAAAAATTTTTAACCCAGCAAAAAACAGGCTCAAACAGCCCCAAACCCATTAAAGGCGATGGCTggggc                                                           >  2:66528/1‑148 (MQ=255)
                                                                                            ccGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACg                                                          <  1:2134368/150‑1 (MQ=255)
                                                                                            ccGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACg                                                          <  2:6190294/150‑1 (MQ=255)
                                                                                             cGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATTTTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACgg                                                         <  2:3765735/150‑1 (MQ=255)
                                                                                               gAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                       <  1:1868976/150‑1 (MQ=255)
                                                                                                  taGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCACTCCCCAGAGGGGCGAGGGGACCCGATTGTGCAAATATTTTTAACCCAGCAACAAACAGGCTCAAACAGCCCCAAACCCTTTCATGGCGATGGCTGGGCCGGTTCAg                                                    >  2:7295330/2‑150 (MQ=38)
                                                                                                    ggCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc                                                  <  1:9201333/150‑1 (MQ=255)
                                                                                                     gCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAcc                                                 <  2:322077/150‑1 (MQ=255)
                                                                                                             aGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGaa                                         <  1:6395821/150‑1 (MQ=255)
                                                                                                               ggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAata                                       <  2:4579212/150‑1 (MQ=255)
                                                                                                                    gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  <  1:7840257/150‑1 (MQ=255)
                                                                                                                    gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  <  1:7980529/150‑1 (MQ=255)
                                                                                                                    gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  <  2:6115357/150‑1 (MQ=255)
                                                                                                                    gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGGTCAGACCTTGCCGAATATtctc                                  >  2:4383893/1‑150 (MQ=255)
                                                                                                                      gcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCa                                >  1:6652684/1‑150 (MQ=255)
                                                                                                                                tCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtctc                      <  1:1998914/150‑1 (MQ=255)
                                                                                                                                  aCCCTAACCCTCTCCCCAGAGGGGCGAGTGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACATTGCCGAATATTCTCCAGCACCGTCTCCa                    <  2:5489772/150‑1 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCAt                   <  2:8148037/150‑1 (MQ=255)
                                                                                                                                     cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt                 >  2:1124512/1‑150 (MQ=255)
                                                                                                                                          ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            <  1:8544223/150‑1 (MQ=255)
                                                                                                                                          ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            <  1:2444543/150‑1 (MQ=255)
                                                                                                                                          ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            <  1:3180304/150‑1 (MQ=255)
                                                                                                                                          ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            <  1:1616541/150‑1 (MQ=255)
                                                                                                                                           ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac           >  1:3700407/1‑150 (MQ=255)
                                                                                                                                           ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac           <  2:3871517/150‑1 (MQ=255)
                                                                                                                                            tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAGTATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc          >  2:6455509/1‑150 (MQ=255)
                                                                                                                                            tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc          >  1:5378183/1‑150 (MQ=255)
                                                                                                                                             ctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacca         <  2:5279671/150‑1 (MQ=255)
                                                                                                                                                cccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaa      >  2:1578880/1‑150 (MQ=255)
                                                                                                                                                cccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCCATGGCTGGGACGGATCAGACCTTGCCGAATATTCTCCAGCACCGTCACCATGTTTTAACCacaa      >  2:409912/1‑150 (MQ=255)
                                                                                                                                                 ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac     >  1:3238203/1‑150 (MQ=255)
                                                                                                                                                 ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac     >  2:472304/1‑150 (MQ=255)
                                                                                                                                                 ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac     >  2:2339509/1‑150 (MQ=255)
                                                                                                                                                  cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca    >  2:8270506/1‑150 (MQ=255)
                                                                                                                                                   agagGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAg   <  2:7120875/150‑1 (MQ=255)
                                                                                                                                                    gagGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGc  <  1:5522293/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351080‑1351377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: