| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 2,561,205 | 0 | A | T | 23.8% | 33.7 / 12.3 | 21 | intergenic (+307/‑163) | yffL/yffM | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base A (8/8); new base T (3/2); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCC > NC_000913_3_bme_pgi/2561102‑2561345 | tcATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCaa < 1:136917/105‑1 (MQ=255)tcATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCaa > 2:136917/1‑105 (MQ=255) gCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACcatcat < 2:8705/150‑1 (MQ=255) gCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACcatcat > 2:42040/1‑150 (MQ=255) ccTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATg > 3:22429/1‑150 (MQ=255) aaaCAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGtt > 3:67402/1‑150 (MQ=255) agaagaATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATtca > 2:19045/1‑97 (MQ=255) agaagaATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATtca < 1:19045/97‑1 (MQ=255) gaagaaTTATTTCGCCGCCCGATAGGTCGGCTAGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAACAAGCTGGAAACGCCCATTCACTCCCGCACAACCTCAACAGCAGACCAACAAACCATCCTGGCATTCGTTCATgg < 4:20356/150‑1 (MQ=255) agaaTTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATgggg < 4:70875/150‑1 (MQ=255) ttCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGt > 1:64115/1‑150 (MQ=255) ccgccTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTatat < 1:42040/150‑1 (MQ=255) cTGGTTGCGAGCATTAACCCTGCTGATTCGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCCCTCACGCCCAACCTGAACAGCAGACCAACAAACCCTCATGGCATTCGTTCATTGGGAGCGCCGTATATCTCAAAGTGTGACTTc < 4:977/150‑1 (MQ=255) gCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATtg > 3:50227/1‑150 (MQ=255) aTTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGc > 1:51593/1‑150 (MQ=255) aCCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGta > 1:99780/1‑115 (MQ=255) aCCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGta < 2:99780/115‑1 (MQ=255) aGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGa < 2:64115/150‑1 (MQ=255) gcgtttCCAGCTTGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGc > 1:55558/4‑127 (MQ=255) gcgtttCCAGCTTGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGc < 2:55558/124‑1 (MQ=255) gtttCCAGCTTGCTGGAAATGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGcc > 1:13809/2‑150 (MQ=255) gtttCCAGCTTGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGTATTCGTTCATGGGGAGCGCGGTATAtc > 1:104008/2‑98 (MQ=255) gtttCCAGCTTGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATAtc < 2:104008/97‑1 (MQ=255) | TCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCC > NC_000913_3_bme_pgi/2561102‑2561345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |