BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	2,662,004	T→A	E43V (GAA→GTA)	iscR ←	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	2,662,004	0	T	A	100.0%	576.4 / NA	150	E43V (GAA→GTA)	iscR	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (80/70); total (80/70)

Only 100 of 152 total aligned reads displayed.

AACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCAC  >  NC_000913_3_KO_pgi/2661858‑2662143

aaCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCa                                                                                                                                          >  4:183298/1‑150 (MQ=255)

   gggTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGAt                                                                                                                                       <  3:275648/150‑1 (MQ=255)

          aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAg                                                                                                                                <  3:887260/150‑1 (MQ=255)

          aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCCGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAg                                                                                                                                <  1:422340/150‑1 (MQ=255)

            cTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAggg                                                                                                                              >  2:943257/1‑150 (MQ=255)

             tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGa                                                                                                                             >  1:1085087/1‑150 (MQ=255)

              aCAGATTCGTCAACGGCGCTAGTTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGaaa                                                                                                                                 <  4:233118/145‑1 (MQ=255)

              aCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGaa                                                                                                                            >  3:766389/1‑150 (MQ=255)

                  aTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTc                                                                                                                        >  4:761023/1‑150 (MQ=255)

                    tCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTccc                                                                                                                      >  2:1093638/1‑150 (MQ=255)

                     cGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCt                                                                                                                     <  4:148967/150‑1 (MQ=255)

                      gTCAACTGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTg                                                                                                                    >  1:896020/1‑150 (MQ=255)

                      gTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTcc                                                                                                                       >  4:418333/1‑147 (MQ=255)

                      gTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTg                                                                                                                    >  1:1536576/1‑150 (MQ=255)

                          aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGt                                                                                                                >  3:663762/1‑150 (MQ=255)

                            ggCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTc                                                                                                              >  2:1493960/1‑150 (MQ=255)

                                cTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaa                                                                                                          <  2:1079257/150‑1 (MQ=255)

                                  aaTTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat                                                                                                        >  3:392663/1‑150 (MQ=255)

                                  aaTTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat                                                                                                        <  1:281676/150‑1 (MQ=255)

                                      aCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa                                                                                                           >  2:633873/1‑143 (MQ=255)

                                      aCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa                                                                                                           <  1:633873/143‑1 (MQ=255)

                                      aCTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa                                                                                                    <  1:834065/150‑1 (MQ=255)

                                       cTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg                                                                                                   >  1:826530/1‑150 (MQ=255)

                                        ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTccc                                                                                                                      >  1:1039653/1‑130 (MQ=255)

                                        ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc                                                                                                  >  3:342027/1‑150 (MQ=255)

                                          cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa                                                                                                >  3:538335/1‑150 (MQ=255)

                                                 ggCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGAc                                                                                                                  <  2:456738/125‑1 (MQ=255)

                                                    gATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCgg                                                                                      <  3:761023/150‑1 (MQ=255)

                                                         tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaa                                                                                                          >  4:941871/1‑125 (MQ=255)

                                                         tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCg                                                                                 >  2:6516/1‑150 (MQ=255)

                                                            tgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt                                                                              >  1:1086901/1‑150 (MQ=255)

                                                              gCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                                                            >  2:54849/1‑150 (MQ=255)

                                                              gCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCa                                                                            >  3:61874/1‑150 (MQ=255)

                                                               cATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTAcc                                                                                                                                           <  2:1341073/86‑1 (MQ=255)

                                                               cATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGACAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTCCag                                                                           >  4:446/1‑150 (MQ=255)

                                                                aTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                                                          >  1:1069283/1‑150 (MQ=255)

                                                                aTCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCaga                                                                          <  2:1536576/150‑1 (MQ=255)

                                                                  cTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGt                                                                        <  1:1093638/150‑1 (MQ=255)

                                                                   tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                                                       >  3:809178/1‑150 (MQ=255)

                                                                   tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                                                       >  3:251238/1‑150 (MQ=255)

                                                                   tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                                                       <  1:735244/150‑1 (MQ=255)

                                                                   cgtGCCCAACAGATAACGACCGCCTGGTTCACGTACGGGGAAATCTAGACGGTTGTTACGCAGACGGGAAAGCAGTTGTACCAGATAAGAAATGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTCCAGAGtt                                                                       <  4:878594/148‑1 (MQ=255)

                                                                     tGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa                                                                     <  2:1412138/150‑1 (MQ=255)

                                                                        cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcg                                                                  >  1:931079/1‑150 (MQ=255)

                                                                        cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcg                                                                  >  4:240345/1‑150 (MQ=255)

                                                                         tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcgc                                                                 >  1:228724/1‑150 (MQ=255)

                                                                         tAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcgc                                                                 >  2:995866/1‑150 (MQ=255)

                                                                         tAACAGATAACCACCGCCTGGTACACGTACGATGGAAACCAGACCATCTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcgc                                                                 >  4:93734/1‑150 (MQ=255)

                                                                          aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCg                                                                                                             <  4:705019/105‑1 (MQ=255)

                                                                          aaCAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                                                       >  1:1454635/1‑143 (MQ=255)

                                                                            cAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAAc                                                              <  4:342027/150‑1 (MQ=255)

                                                                               aTAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTc                                                           >  1:1496510/1‑150 (MQ=255)

                                                                                 aaCCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCaa                                                         <  2:339484/150‑1 (MQ=255)

                                                                                   ccaccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGc                                                       >  1:1056213/1‑150 (MQ=255)

                                                                                      ccGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAtt                                                    >  2:1662479/1‑150 (MQ=255)

                                                                                      ccGCCTGGTCCACGTACGCTGGAAACCAAACCATTTTTCCGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAtt                                                    <  1:1109844/150‑1 (MQ=255)

                                                                                        gCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATCTCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGc                                                  >  2:1047845/1‑150 (MQ=255)

                                                                                        gCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGc                                                  <  1:128239/150‑1 (MQ=255)

                                                                                            ggTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg                                                                    <  3:974616/128‑1 (MQ=255)

                                                                                            ggTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAg                                                                    >  4:974616/1‑128 (MQ=255)

                                                                                               ccACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                                                       >  1:1166911/1‑122 (MQ=255)

                                                                                               ccACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACg                                           >  2:1225632/1‑150 (MQ=255)

                                                                                                cACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg                                          >  2:1173191/1‑150 (MQ=255)

                                                                                                  cGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCa                                        <  1:995866/150‑1 (MQ=255)

                                                                                                   gTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCAt                                       <  1:54849/150‑1 (MQ=255)

                                                                                                   gTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCAt                                       <  1:358986/150‑1 (MQ=255)

                                                                                                        cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAgcgc                                  >  2:680613/1‑150 (MQ=255)

                                                                                                         tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGcc                                 <  3:240394/150‑1 (MQ=255)

                                                                                                         tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGcc                                 >  3:291155/1‑150 (MQ=255)

                                                                                                          ggAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGccc                                <  3:263640/150‑1 (MQ=255)

                                                                                                              aCCATATCATTTGTACGCAGACGGGAAAACAGTAGTACCAGATAAGAAAGGAAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCATCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTa                            <  2:1083868/150‑1 (MQ=255)

                                                                                                              aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCCGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTa                            <  1:18507/150‑1 (MQ=255)

                                                                                                              aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTa                            <  4:309936/150‑1 (MQ=255)

                                                                                                               ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTa                            >  1:1041103/1‑149 (MQ=255)

                                                                                                               ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTa                            <  2:1041103/149‑1 (MQ=255)

                                                                                                               ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCAAATGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCTGTCACGGCATAGCGCCCTTTAg                           <  2:345589/150‑1 (MQ=255)

                                                                                                                 aGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg                                                   <  2:636985/124‑1 (MQ=255)

                                                                                                                 aGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg                                                   >  1:636985/1‑124 (MQ=255)

                                                                                                                 aGACCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGAt                         <  3:793176/150‑1 (MQ=255)

                                                                                                                 aGACCATTTTTACGAAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGAt                         <  2:836073/150‑1 (MQ=255)

                                                                                                                   aCCATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGACATAGCGCCCttt                             <  1:1188892/144‑1 (MQ=255)

                                                                                                                    ccATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATgcc                      >  3:847581/1‑148 (MQ=255)

                                                                                                                    ccATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTc                      >  2:1129582/1‑150 (MQ=255)

                                                                                                                     cATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCa                     >  2:1650220/1‑150 (MQ=255)

                                                                                                                     cATTTTTACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCa                     <  3:984964/150‑1 (MQ=255)

                                                                                                                          ttACGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGtctc                <  2:1276950/150‑1 (MQ=255)

                                                                                                                            aCGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCAt              <  4:663762/150‑1 (MQ=255)

                                                                                                                            aCGCAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCAt              <  3:240345/150‑1 (MQ=255)

                                                                                                                               cAGACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTc           <  2:1086920/150‑1 (MQ=255)

                                                                                                                                 gACGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCtt         <  3:201143/150‑1 (MQ=255)

                                                                                                                                  aCGGGAAAACAGTTGTACCAGATAAGAAATGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTa        >  2:1160148/1‑150 (MQ=255)

                                                                                                                                  aCGGGAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTa        <  2:235551/150‑1 (MQ=255)

                                                                                                                                      gAAAACTGTTGTAGTAGATAAGAGAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGGTGAGCGCAACGTCAAGCATTTCGGTCACGGCATAGTGACATTGAGATGTCAGGCTCATGTCTTACtgc    >  4:423081/1‑148 (MQ=255)

                                                                                                                                      gAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAg                           >  3:227590/1‑127 (MQ=255)

                                                                                                                                      gAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTc    >  1:49194/1‑150 (MQ=255)

                                                                                                                                      gAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTc    >  1:1331778/1‑150 (MQ=255)

                                                                                                                                      gAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTc    >  4:506660/1‑150 (MQ=255)

                                                                                                                                      gAAAACAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTc    >  1:851909/1‑150 (MQ=255)

                                                                                                                                        aaaCAGTTGTACCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCAc  <  2:116889/150‑1 (MQ=255)

                                                                                                                                             gttgtaCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCtt                              >  2:782568/1‑117 (MQ=255)

AACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCAC  >  NC_000913_3_KO_pgi/2661858‑2662143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`