| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_pgi | 4,234,667 | Δ1 bp | intergenic (‑1434/‑499) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 4,234,666 | 0 | A | . | 92.3% | 41.2 / ‑2.7 | 13 | intergenic (‑1433/‑500) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (6/6); minor base C (0/1); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAA > NC_000913_3_pae_pgi/4234542‑4234795 | aCCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATa > 4:116760/1‑150 (MQ=255) ttttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGAATTGAAGAGGCGCTAGTAGTG‑ATCATAGCCGATTTGTAAGCCGGATAAGGCGTTCAcgc > 1:140411/1‑150 (MQ=255) aCGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCGTGCAGT‑CATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc < 1:116060/150‑1 (MQ=255) tgtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc < 4:119417/150‑1 (MQ=255) ggCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa > 1:33731/1‑150 (MQ=255) cGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCgg < 2:33731/150‑2 (MQ=255) cGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc < 3:116760/150‑1 (MQ=255) aTCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCATGCCTACAGGTCGATGc < 2:122130/150‑1 (MQ=255) gggCCAGCCCGAGAATAGAGGAATTGAAGAGGCGCTTGGAGTG‑ATGAGCGTCGATATGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCAACCGATGCCGGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat < 2:175277/149‑1 (MQ=25) ccACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑CTCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTa > 2:68592/1‑150 (MQ=255) aTTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa > 3:5750/1‑150 (MQ=255) tGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc < 3:21474/45‑1 (MQ=21) tGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc > 4:21474/1‑45 (MQ=21) | ACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAA > NC_000913_3_pae_pgi/4234542‑4234795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |