| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 3,342,316 | 0 | T | A | 35.7% | 10.1 / 12.0 | 14 | V15D (GTC→GAC) | kdsC | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (5/4); new base A (3/2); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCATTCGTT > NC_000913_3_pae_pgi/3342209‑3342462 | gtgtgtTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgct > 2:199395/1‑150 (MQ=255) tGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACt < 1:199395/150‑1 (MQ=255) aacaaTGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGa > 3:83242/1‑150 (MQ=255) cAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGCGCTGAAAGCGt < 4:83242/150‑1 (MQ=255) gCGTCGCTTGCGACCTGTTACGGCGCTGACAGGGCCg > 3:93609/1‑37 (MQ=21) gCGTCGCTTGCGACCTGTTACGGCGCTGACAGGGCCg < 4:93609/37‑1 (MQ=18) cgtcGGCGCTGACAGGGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATgg > 4:96995/4‑150 (MQ=255) gtcGGCGCTGACAGGGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCaataa < 3:33896/105‑1 (MQ=255) gtcGGCGCTGACAGGGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCaataa > 4:33896/3‑107 (MQ=255) ggCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCAtt > 3:23768/1‑150 (MQ=255) ggCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCAtt > 4:85777/1‑150 (MQ=255) cTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCATTCGtt < 4:23768/150‑1 (MQ=255) tGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGCTGGcc > 4:37081/1‑80 (MQ=255) tGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGcc < 3:37081/80‑1 (MQ=255) | GTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCATTCGTT > NC_000913_3_pae_pgi/3342209‑3342462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |