| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 3,342,320 | 0 | C | G | 33.3% | 20.7 / 10.1 | 15 | S16R (AGC→AGG) | kdsC | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (6/4); new base G (3/2); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCATTCGTTGTGCG > NC_000913_3_pae_pgi/3342209‑3342467 | gtgtgtTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTctgct > 2:199395/1‑150 (MQ=255) tGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACt < 1:199395/150‑1 (MQ=255) aacaaTGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGa > 3:83242/1‑150 (MQ=255) cAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGCGCTGAAAGCGt < 4:83242/150‑1 (MQ=255) gCGTCGCTTGCGACCTGTTACGGCGCTGACAGGGCCg > 3:93609/1‑37 (MQ=21) gCGTCGCTTGCGACCTGTTACGGCGCTGACAGGGCCg < 4:93609/37‑1 (MQ=18) cgtcGGCGCTGACAGGGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATgg > 4:96995/4‑150 (MQ=255) gtcGGCGCTGACAGGGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCaataa < 3:33896/105‑1 (MQ=255) gtcGGCGCTGACAGGGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCaataa > 4:33896/3‑107 (MQ=255) ggCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCAtt > 3:23768/1‑150 (MQ=255) ggCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCAtt > 4:85777/1‑150 (MQ=255) cTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCATTCGtt < 4:23768/150‑1 (MQ=255) tGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGCTGGcc > 4:37081/1‑80 (MQ=255) tGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGcc < 3:37081/80‑1 (MQ=255) aGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCATTCGTTGTgcg > 2:111282/1‑150 (MQ=255) | GTGTGTTACATATGCATGATTTACTGCGTGCAGGCGTAGTGTAAAGATTCAAGGATAAACAACAATGAGCAAAGCAGGTGCGTCGCTTGCGACCTGTTACGGCCCTGTCAGCGCCGACGTTATAGCAAAAGCAGAGAACATTCGTCTGCTGATCCTCGATGTCGATGGCGTACTGTCAGATGGCCTGATTTATATGGGCAATAATGGCGAAGAGCTGAAAGCGTTCAATGTTCGTGACGGTTATGGCATTCGTTGTGCG > NC_000913_3_pae_pgi/3342209‑3342467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |