Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 4,233,804 1557 bp→GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT coding (47‑1603/1650 nt) pgi → glucosephosphate isomerase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4233804 4235360 1557 88 [0] [0] 65 pgi glucosephosphate isomerase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 42338030 (0.000)52 (0.630)
+GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT
40/334 0.8 100% coding (46/1650 nt) pgi glucosephosphate isomerase
?NC_000913 4235361 = 0 (0.000)coding (1604/1650 nt) pgi glucosephosphate isomerase

CGCGAAGTGGTTGAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAAT  >  NC_000913/4235066‑4235659
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
tttttcatccactcctggcggtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTAGa                                                                                                                                                                                                                                                                                                       >  1:93730‑M2/296‑301 (MQ=255)
       tccactcctggcggtcagtagttcagctaataaatgcttcactgcgctaagggtttacacgcaacattacgcaaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattcacaaaatcagaagagtattgcgaatgaaaaacatcaatccaacgcataccgctgccttgcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAAtgt                                                                                                                                                                                                                                                                                                <  1:126232‑M2/13‑2 (MQ=255)
           ctcctggcggtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagcataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGt                                                                                                                                                                                                                                                                                               >  1:724978‑M2/285‑298 (MQ=255)
                    gtcagtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCg                                                                                                                                                                                                                                                                                    >  1:701597‑M2/276‑300 (MQ=255)
                        gtagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCtata                                                                                                                                                                                                                                                                               >  1:351985‑M2/272‑301 (MQ=255)
                         tagttcagctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATaa                                                                                                                                                                                                                                                                              <  1:457451‑M2/31‑1 (MQ=255)
                           tttcagctaataaatgcttcactgcgctaagggtttacacgcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAg                                                                                                                                                                                                                                                                            <  1:301306‑M2/33‑1 (MQ=255)
                                gctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatctccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTgg                                                                                                                                                                                                                                                                       >  1:297476‑M2/264‑301 (MQ=255)
                                gctaataaatgcttcactgcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATTGTCTGATTAACCGCTATAAAGCGTgg                                                                                                                                                                                                                                                                       >  1:253278‑M2/264‑301 (MQ=255)
                                             tcaatgcgctaagtgtgtacactcaacataaagctaacggcactaaaaccaccacatttttctgtgactggcgctacaatctgccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatcat                                                                                                                                                                                                                                                          <  1:501832‑M2/51‑1 (MQ=255)
                                                  gcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGgttaatcatctccg                                                                                                                                                                                                                                                     >  1:521126‑M2/246‑297 (MQ=255)
                                                  gcgctaagggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCAtcgtcg                                                                                                                                                                                                                                                     >  1:469459‑M2/246‑301 (MQ=255)
                                                          ggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                               <  1:64156‑M2/62‑1 (MQ=255)
                                                          ggtttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAg                                                                                                                                                                                                                                             >  1:268234‑M2/238‑301 (MQ=255)
                                                             ttacactcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtnggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGcc                                                                                                                                                                                                                                          <  1:1112001‑M2/67‑1 (MQ=255)
                                                                      acattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGAAAaggg                                                                                                                                                                                                                                 >  1:825390‑M2/226‑300 (MQ=255)
                                                                                            accatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTc                                                                                                                                                                                                                                                                                              <  2:514279‑M2/15‑1 (MQ=255)
                                                                                                   cattgtgctgtgactggcgctacaatcatccaaagtcacaattctaaaaatcagaagagtaatgctgatgaaaaacataaatccaacgcaggccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttactatgctttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATAGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCt                                                                                                                                                                                                    <  1:269109‑M2/105‑1 (MQ=255)
                                                                                                      ntttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCg                                                                                                                                                                                                                                                                                    <  2:1041791‑M2/25‑1 (MQ=255)
                                                                                                        ttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCg                                                                                                                                                                                                                                                                                    >  2:590650‑M2/192‑216 (MQ=255)
                                                                                                        ttccttcactggcgctacaatcttccaaagtcacaattctcaaaatcacaagagtattgctaattaaaaccatcaatccaacgcagacccctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatcggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                                                                                                                                                                               <  1:377973‑M2/110‑1 (MQ=255)
                                                                                                               nctggcgctagaatcttccacagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaagccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGc                                                                                                                                                                                                                                                                           <  2:972102‑M2/34‑1 (MQ=255)
                                                                                                                ctggcgctacaatcttccaaagtcacaattctcaaaatcagaaaagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                                                                                                                                       <  1:182501‑M2/118‑1 (MQ=255)
                                                                                                                           atcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTa                                                                                                                                                                                                                                                               >  2:639812‑M2/173‑218 (MQ=255)
                                                                                                                              ttccaaagtcacaattctcaaaagcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatc                                                                                                                                                                                                                                                            <  2:293663‑M2/49‑1 (MQ=255)
                                                                                                                                ncaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatcat                                                                                                                                                                                                                                                          <  2:1133862‑M2/51‑1 (MQ=255)
                                                                                                                                ncaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAatcat                                                                                                                                                                                                                                                          <  2:1081105‑M2/51‑1 (MQ=255)
                                                                                                                                           attctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGt                                                                                                                                                                                                                                               <  2:351976‑M2/62‑1 (MQ=255)
                                                                                                                                               tcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGc                                                                                                                                                                                                                                           <  2:253272‑M2/66‑1 (MQ=255)
                                                                                                                                                caaaatcagaagagtattgctaatgaaagacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGcc                                                                                                                                                                                                                                          <  2:286314‑M2/67‑1 (MQ=255)
                                                                                                                                                  aaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                                                                                                                                     <  1:100351‑M2/152‑1 (MQ=255)
                                                                                                                                                   aatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGa                                                                                                                                                                                                                                       >  2:88437‑M2/149‑218 (MQ=255)
                                                                                                                                                    atcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                                                                                                                                   <  1:894016‑M2/154‑1 (MQ=255)
                                                                                                                                                    atcagaagagtaatgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGAt                                                                                                                                                                                                                                      <  2:297468‑M2/71‑1 (MQ=255)
                                                                                                                                                      cagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTa                                                                                                                                                 >  1:595917‑M2/146‑301 (MQ=255)
                                                                                                                                                         aagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcaagacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                <  1:385019‑M2/157‑1 (MQ=255)
                                                                                                                                                             gtattgctaatgaaaaacctcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggctccccggaatGCTCGACCAATGGTCTGATTAACCGCTATACAGCGTGGCGCGGTTAATCATCGTCGATATGTATGCCGGATAAGTCgttn                                                                                                                                                                                                                             >  2:1010185‑M2/139‑217 (MQ=255)
                                                                                                                                                             gtattgccaatgaaaaacatcaatccaacgcagaccgcggcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                                                                                                                                                     <  1:1010226‑M2/104‑1 (MQ=255)
                                                                                                                                                              tattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGt                                                                                                                                           <  1:592064‑M2/162‑1 (MQ=255)
                                                                                                                                                                   ctaatgaaaaacatcaatccaacgcagaccgctgccgggcaggcactacgttgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                    <  1:517073‑M2/169‑1 (MQ=255)
                                                                                                                                                                        gaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGTCAATTa                                                                                                                               >  1:472222‑M2/128‑301 (MQ=255)
                                                                                                                                                                              catcaatccaacgcggcccgcggcctggcaggcactacggggtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAcag                                                                                                                         <  1:155128‑M2/180‑2 (MQ=255)
                                                                                                                                                                              catcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                                         <  1:639829‑M2/180‑1 (MQ=255)
                                                                                                                                                                                   atccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGatn                                                                                                                                                                                                       >  2:851438‑M2/117‑217 (MQ=255)
                                                                                                                                                                                   atccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                <  1:851468‑M2/157‑1 (MQ=255)
                                                                                                                                                                                     ccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                                                                                                                                                     <  2:724957‑M2/104‑1 (MQ=255)
                                                                                                                                                                                                cgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATa                                                                                                       <  1:590665‑M2/198‑1 (MQ=255)
                                                                                                                                                                                                         gcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                                                                                                                                  <  2:624032‑M2/123‑1 (MQ=255)
                                                                                                                                                                                                             gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACaa                                                                                          >  1:395204‑M2/91‑301 (MQ=255)
                                                                                                                                                                                                             gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAAATAGCACGATTGATTAAATAACCTTAATAACaa                                                                                          >  1:990169‑M2/91‑301 (MQ=255)
                                                                                                                                                                                                              cactacggtgtagtctggagctgcttcgaagttcctatacttgctagagaataggaacttcgagctgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                            <  2:93726‑M2/129‑1 (MQ=255)
                                                                                                                                                                                                              cactacggtgtaggctggagctgcttcgaagttcctatactttcgagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                                                                                                                            <  2:320522‑M2/129‑1 (MQ=255)
                                                                                                                                                                                                                                          aagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                                                                                                                                <  2:493204‑M2/157‑1 (MQ=255)
                                                                                                                                                                                                                                          aagttcctagagttgctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATc                                                             <  1:88441‑M2/240‑1 (MQ=255)
                                                                                                                                                                                                                                            attcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATcaa                                                           >  1:132491‑M2/60‑300 (MQ=255)
                                                                                                                                                                                                                                                    actttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                                                                                                                                   >  1:273192‑M2/52‑205 (MQ=255)
                                                                                                                                                                                                                                                    actttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                                                                                                                                   <  2:273185‑M2/154‑1 (MQ=255)
                                                                                                                                                                                                                                                      tttctagagaataggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                                                                                                                                    <  2:268227‑M2/169‑1 (MQ=255)
                                                                                                                                                                                                                                                         atagagaataggaagttcgcactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCAGCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTg                                              <  1:810328‑M2/255‑1 (MQ=255)
                                                                                                                                                                                                                                                                 taggaacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                                                                                                                         <  2:593436‑M2/180‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gaccttcgaactgcagccagaaggatcctcggagtGCTCGACCAATGTGCTGATTTACCGCTATCACGCGTGGCGCGATTAATCATCTGCGATTTGTAGGCCGGATAAGTCGTTCCCGCCGCATCCGGCAACCGTGGCCTGATGCGACGCGGTCGNGGCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAAttt                                    <  1:1109238‑M2/265‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aacttcgaactgcaggtcgacggatccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa                                                                                                                     <  2:472210‑M2/184‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            atccccggaatGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTtaata                                                                                              <  2:469447‑M2/207‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               cccggaatGCTCGACCAATGGGCTTATTAACCGCTATAAAGCGGGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCTCATCCGGCAACCGATGCCAGATGCGACGCGGTCGCGTCTTATCAGTCCTACAGGTCGATGCCNATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATcc        <  1:1120232‑M2/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     atGCTCGACCAATTGGTTGATTAACCTGTATAAAGAGTGGATCGGTTAATCTGCGTCGATATGTAGGCCTGATAAGGCGTTCATGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAAt  <  1:666286‑M2/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
CGCGAAGTGGTTGAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAAT  >  NC_000913/4235066‑4235659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: