BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,513,975	C→T	5.2%	R54Q (CGA→CAA)	nreA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,513,975	0	C	T	5.2%	578.5 / 11.1	178	R54Q (CGA→CAA)	nreA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (87/81); new base T (6/4); total (93/85)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

TTCGCTCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCTT  >  CP000730/2513836‑2514113

ttCGCTCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCg                                                                                                                                           <  1:357389/141‑1 (MQ=255)

       aaTAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTAttt                                                                                                                                    >  2:717948/1‑141 (MQ=255)

       aaTAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTAttt                                                                                                                                    <  1:195810/141‑1 (MQ=255)

       aaTAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTAttt                                                                                                                                    <  1:1234034/141‑1 (MQ=255)

        aTAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCGATTTAAATTGATTAtttt                                                                                                                                   <  2:952848/141‑1 (MQ=255)

         tAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCGATTTAAATTGATTAtttta                                                                                                                                  <  1:454969/141‑2 (MQ=255)

          aaaaTTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTtta                                                                                                                                 <  1:1254433/141‑1 (MQ=255)

          aaaaTTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTtta                                                                                                                                 >  1:295445/1‑141 (MQ=255)

           aaaTTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTttat                                                                                                                                <  2:1092556/141‑1 (MQ=255)

            aaTTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCGATTTAAATTGATTAttttaaga                                                                                                                               <  2:181357/141‑5 (MQ=255)

            aaTTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTttatt                                                                                                                               >  2:1225676/1‑141 (MQ=255)

             aTTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTttatta                                                                                                                              <  1:492712/141‑1 (MQ=255)

              ttGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTAc                                                                                                                             >  2:1371876/1‑141 (MQ=255)

                ggATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCt                                                                                                                           >  1:321090/1‑141 (MQ=255)

                 gATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTg                                                                                                                          <  1:91594/141‑1 (MQ=255)

                 gATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTg                                                                                                                          <  1:585002/141‑1 (MQ=255)

                     gTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCAAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAAc                                                                                                                      >  1:449945/1‑141 (MQ=255)

                       cAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACAt                                                                                                                    <  1:996419/141‑1 (MQ=255)

                          tCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGt                                                                                                                 >  1:681817/1‑141 (MQ=255)

                            ttttCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGc                                                                                                               <  1:235791/141‑1 (MQ=255)

                            ttttCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGc                                                                                                               <  1:873075/141‑1 (MQ=255)

                               tcttcAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCAt                                                                                                            >  2:1555316/1‑141 (MQ=255)

                                 ttcAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCAttt                                                                                                          >  1:547590/1‑141 (MQ=255)

                                  tcAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCAtttt                                                                                                         <  2:1150711/141‑1 (MQ=255)

                                   cAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTa                                                                                                        >  2:42434/1‑141 (MQ=255)

                                        ccTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGga                                                                                                   >  1:912319/1‑141 (MQ=255)

                                          tAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGgaga                                                                                                 <  1:1468582/141‑1 (MQ=255)

                                          tAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGgaga                                                                                                 <  2:749848/141‑1 (MQ=255)

                                             aGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatga                                                                                              <  1:1080325/141‑1 (MQ=255)

                                                  aaCCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATCGTGCCATTTTATTGGAGATGATGGtt                                                                                         >  1:403587/1‑141 (MQ=255)

                                                   aCCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGttt                                                                                        >  2:149174/1‑141 (MQ=255)

                                                   aCCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGttt                                                                                        <  1:688875/141‑1 (MQ=255)

                                                    ccAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGcc                                                                                                              >  2:791645/1‑118 (MQ=255)

                                                    ccAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGcc                                                                                                              <  1:791645/118‑1 (MQ=255)

                                                    ccAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTg                                                                                       >  2:217765/1‑141 (MQ=255)

                                                     cAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCAAGACCATTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAAATAATGtttttt                                                                                      >  2:493428/1‑139 (MQ=255)

                                                      aaCATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGtt                                                                                     <  1:1367652/141‑1 (MQ=255)

                                                       aCATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTa                                                                                    >  1:680731/1‑141 (MQ=255)

                                                        cATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAg                                                                                   >  1:1394133/1‑141 (MQ=255)

                                                            ggCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTc                                                                               >  2:1011223/1‑141 (MQ=255)

                                                             gCTATTACCATACGCTTTCCTGTTGTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCa                                                                              >  2:1005798/1‑141 (MQ=255)

                                                              cTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCAt                                                                             <  2:66480/141‑1 (MQ=255)

                                                              cTATTACCATACGCTTTCCTGTTTTCATAACAGTTACAGCTAATACACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTGTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCAt                                                                             >  2:1319406/1‑141 (MQ=255)

                                                               tATTACCATACGCTTTCCTGTTTTCATAACAGCTCCAGCCAATCCACGACCTTTTCTTAAAATACTCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATa                                                                            >  1:1636523/1‑141 (MQ=255)

                                                                  tACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAGATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGaa                                                                         >  2:654468/1‑141 (MQ=255)

                                                                  tACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGaa                                                                         <  1:284955/141‑1 (MQ=255)

                                                                    ccATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatgat                                                                                             >  1:1374988/1‑119 (MQ=255)

                                                                      aTACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCg                                                                     >  2:1262426/1‑141 (MQ=255)

                                                                        aCGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGag                                                                   <  2:595899/141‑2 (MQ=255)

                                                                         cGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGAtt                                                                  <  1:217765/141‑1 (MQ=255)

                                                                         cGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCGTAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGAtt                                                                  >  1:326624/1‑141 (MQ=255)

                                                                            tttCCTGTTTTCATAACAGTTCCAGCTGATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGcc                                                               <  1:1411980/141‑1 (MQ=255)

                                                                             ttCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCg                                                              <  1:82830/141‑1 (MQ=255)

                                                                             ttCCTGTTTTCATAACAGCTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCg                                                              >  2:546356/1‑141 (MQ=255)

                                                                                  gTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCaaaa                                                         >  2:1435941/1‑141 (MQ=255)

                                                                                          aaCAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGt                                                                                                                 >  1:1036592/1‑77 (MQ=255)

                                                                                          aaCAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGt                                                                                                                 <  2:1036592/77‑1 (MQ=255)

                                                                                          aaCAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAAcc                                                 <  1:717948/141‑1 (MQ=255)

                                                                                           aCAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAAccc                                                <  2:547590/141‑1 (MQ=255)

                                                                                            cAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCct                                               >  1:1121735/1‑141 (MQ=255)

                                                                                            cAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCct                                               <  1:120535/141‑1 (MQ=255)

                                                                                            cAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCct                                               >  1:48522/1‑141 (MQ=255)

                                                                                             aGTTCCAGCTAATCCACGGCCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCctc                                              >  2:479763/1‑141 (MQ=255)

                                                                                                 ccAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCtcttct                                          <  2:1641713/141‑1 (MQ=255)

                                                                                                  cAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTcttctt                                         >  1:518860/1‑141 (MQ=255)

                                                                                                   aGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCttt                                        >  1:25407/1‑141 (MQ=255)

                                                                                                   aGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCttt                                        >  2:1537680/1‑141 (MQ=255)

                                                                                                   aGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCttt                                        >  1:70349/1‑141 (MQ=255)

                                                                                                   aGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCttt                                        <  1:810393/141‑1 (MQ=255)

                                                                                                     cTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGGAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGc                                      <  2:129893/141‑1 (MQ=255)

                                                                                                     cTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGc                                      <  1:1353965/141‑1 (MQ=255)

                                                                                                        aTCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTa                                   >  2:77935/1‑141 (MQ=255)

                                                                                                        aTCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTCTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTa                                   >  2:1589839/1‑141 (MQ=255)

                                                                                                          ccACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTAtt                                 <  1:173949/141‑1 (MQ=255)

                                                                                                               aCCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATATTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATc                            <  2:227596/141‑1 (MQ=255)

                                                                                                                ccTTTTCTTAGAATAATCAATTTAAATCTATTATTTTTATTACCTGAAACATAGTGCCATTTGATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCt                           <  2:1422184/141‑1 (MQ=255)

                                                                                                                ccTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCt                           >  2:897190/1‑141 (MQ=255)

                                                                                                                 cTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTa                          >  2:334565/1‑141 (MQ=255)

                                                                                                                  ggtaataaaaaTAATCGATTTAAATTGATTATTTTTATTAACTGAAACATAGTGCTATTTTATTGGAGATGTTGGTTTGTTAGATTCATAGAAAGCGATTGCCACAAAATCATAATCCTCTTCTTTGCGTAGTTTATCTaa                         >  2:1215752/8‑141 (MQ=37)

                                                                                                                    ttCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATg                       <  1:149174/141‑1 (MQ=255)

                                                                                                                     tCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGt                      >  2:540275/1‑141 (MQ=255)

                                                                                                                         aaaaTAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGtctca                  >  1:760837/1‑140 (MQ=255)

                                                                                                                          aaaTAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCtt                 <  2:287177/141‑1 (MQ=255)

                                                                                                                            ataatCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGa               <  2:822803/141‑1 (MQ=255)

                                                                                                                               atCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATGACCCTCTTCTTTGCGTATTTTAGCTAATGTCTCTTGAAAt            >  1:575651/1‑141 (MQ=255)

                                                                                                                                tCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAg                                                                       <  2:435499/81‑1 (MQ=255)

                                                                                                                                tCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAg                                                                       >  1:435499/1‑81 (MQ=255)

                                                                                                                                tCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATc           <  1:1045188/141‑1 (MQ=255)

                                                                                                                                tCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATc           >  1:1430300/1‑141 (MQ=255)

                                                                                                                                 cgaTTTAAATTGATTATTTTTATTACCTGAAACATAGTGCCAATTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCt          >  2:408499/3‑141 (MQ=255)

                                                                                                                                  gaTTTAAATTGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTg                >  1:1375377/2‑134 (MQ=255)

                                                                                                                                  gaTTTAAATTGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTg                <  2:1375377/133‑1 (MQ=255)

                                                                                                                                  aaTTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTa         >  1:431715/1‑141 (MQ=255)

                                                                                                                                  aaTTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTa         >  1:959713/1‑141 (MQ=255)

                                                                                                                                  aaTTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTa         >  1:526267/1‑141 (MQ=255)

                                                                                                                                      tAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGAt     <  1:1608284/141‑1 (MQ=255)

                                                                                                                                       aaaTCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATc    <  2:1394133/141‑1 (MQ=255)

                                                                                                                                       aaaTCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATc    >  2:1431003/1‑141 (MQ=255)

                                                                                                                                         aTCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTg                                                                                                     >  2:819865/1‑42 (MQ=255)

                                                                                                                                         aTCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCtt  >  2:886445/1‑141 (MQ=255)

TTCGCTCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCTT  >  CP000730/2513836‑2514113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`