Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 942,230 C→T 6.5% intergenic (‑201/+48) USA300HOU_0905 ← / ← mnhG2 hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730942,2300CT 6.5% 436.1 / 14.0 139intergenic (‑201/+48)USA300HOU_0905/mnhG2hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G
Reads supporting (aligned to +/- strand):  ref base C (65/64);  new base T (7/2);  total (73/66)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

Only 100 of 149 total aligned reads displayed.

TACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCATAA  >  CP000730/942090‑942369
                                                                                                                                             |                                                                                                                                           
   aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa                                                                                                                                          >  2:359720/1‑141 (MQ=255)
      aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTActg                                                                                                                                       <  2:1530055/141‑1 (MQ=38)
      aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg                                                                                                                                       >  2:1190374/1‑141 (MQ=255)
      aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg                                                                                                                                       <  2:183094/141‑1 (MQ=255)
       cGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATATGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAACTGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTActgc                                                                                                                                      <  2:153209/141‑1 (MQ=37)
         aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActgctg                                                                                                                                    >  2:732198/1‑141 (MQ=255)
         aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActgctg                                                                                                                                    <  2:872434/141‑1 (MQ=255)
            tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgttt                                                                                                                                 >  2:187834/1‑141 (MQ=255)
             gAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTTACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttg                                                                                                                                <  1:486325/141‑1 (MQ=255)
             gAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttg                                                                                                                                <  1:273724/141‑1 (MQ=255)
             gAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttg                                                                                                                                <  1:438324/141‑1 (MQ=255)
                  gCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgt                                                                                                                            <  2:553887/141‑1 (MQ=255)
                    aattaaattaAAGAGATATATGTAGATAGGGCGAATCTGTAGTAAAAAAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGa                                                                                                                         <  2:1268467/141‑1 (MQ=255)
                      ttaaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGAtt                                                                                                                       >  1:872921/1‑141 (MQ=255)
                       taaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGAttt                                                                                                                      >  2:1036357/1‑141 (MQ=255)
                       taaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGAttt                                                                                                                      >  1:217849/1‑141 (MQ=255)
                            taaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATAtt                                                                                                                 <  2:663884/141‑1 (MQ=255)
                             aaaGATATATATGTAGATAGGGCGAATCAGAAGAAAAAAAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTc                                                                                                                <  2:63834/141‑1 (MQ=255)
                             aaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTc                                                                                                                <  2:1154046/141‑1 (MQ=255)
                               agagATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCAt                                                                                                              >  1:741152/1‑141 (MQ=255)
                                   atatatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAtt                                                                                                          >  1:1208909/1‑141 (MQ=255)
                                   atatatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAtt                                                                                                          >  1:70091/1‑141 (MQ=255)
                                    tatatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAttt                                                                                                         >  2:1569205/1‑141 (MQ=255)
                                       atGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAGACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGt                                                                                                      <  1:83482/141‑1 (MQ=255)
                                           aGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATGATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTtata                                                                                                  >  2:855535/1‑141 (MQ=255)
                                           aGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTtata                                                                                                  >  1:67736/1‑141 (MQ=255)
                                            gATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTtatat                                                                                                 >  2:1134142/1‑141 (MQ=255)
                                            gATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTtatat                                                                                                 >  1:16036/1‑141 (MQ=255)
                                                gggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc                                                                                             <  1:933027/141‑1 (MQ=255)
                                                gggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc                                                                                             <  2:1074441/141‑1 (MQ=255)
                                                 ggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCt                                                                                            <  2:341537/141‑1 (MQ=255)
                                                  gCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa                                                                                           >  1:127139/1‑141 (MQ=255)
                                                  gCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa                                                                                           >  2:926134/1‑141 (MQ=255)
                                                   cGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACATTGTCAAGAGAAGACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTAc                                                                                          <  1:1396943/141‑1 (MQ=255)
                                                     aaTCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACaa                                                                                        <  1:1214347/141‑1 (MQ=255)
                                                      aTCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAt                                                                                       >  2:519241/1‑141 (MQ=255)
                                                           tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                                  >  1:1006456/1‑141 (MQ=255)
                                                           tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                                  <  2:618093/141‑1 (MQ=255)
                                                           tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                                  <  1:300787/141‑1 (MQ=255)
                                                                  aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                                           <  1:798873/141‑1 (MQ=255)
                                                                  aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                                           <  1:767857/141‑1 (MQ=255)
                                                                  aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATATTACAGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                                           >  2:1513493/1‑141 (MQ=255)
                                                                     aaaaaTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAg                                                                        <  1:1499062/141‑1 (MQ=255)
                                                                         aTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCtt                                                                    <  1:971993/141‑1 (MQ=255)
                                                                         aTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCtt                                                                    >  2:119698/1‑141 (MQ=255)
                                                                         aTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCtt                                                                    >  2:1419638/1‑141 (MQ=255)
                                                                          tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc                                                                   >  2:1404787/1‑141 (MQ=255)
                                                                          tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc                                                                   >  2:594281/1‑141 (MQ=255)
                                                                             ttGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGa                                                                <  2:1284380/141‑1 (MQ=255)
                                                                                     agagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCa                                                         <  2:966474/141‑1 (MQ=255)
                                                                                       agGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATc                                                       >  1:1517575/1‑141 (MQ=255)
                                                                                          tAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGAc                                                    >  2:137321/1‑141 (MQ=255)
                                                                                             cAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACttt                                                 <  2:602497/141‑1 (MQ=255)
                                                                                              aaTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATGATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTa                                                <  1:748865/141‑1 (MQ=255)
                                                                                              aaTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTa                                                <  2:904614/141‑1 (MQ=255)
                                                                                               aTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAg                                               <  2:67736/141‑1 (MQ=255)
                                                                                                    aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt                                          >  2:431075/1‑141 (MQ=255)
                                                                                                    aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt                                          >  1:1067560/1‑141 (MQ=255)
                                                                                                    aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt                                          >  1:254902/1‑141 (MQ=255)
                                                                                                     aaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCtttt                                         >  2:627192/1‑141 (MQ=255)
                                                                                                     aaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCtttt                                         <  2:217849/141‑1 (MQ=255)
                                                                                                          aaaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTc                                             <  2:1405353/132‑1 (MQ=255)
                                                                                                          aaaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGta                                    <  2:750021/141‑1 (MQ=255)
                                                                                                           aaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGtat                                   <  2:870556/141‑1 (MQ=255)
                                                                                                            aCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGtata                                  >  2:227611/1‑141 (MQ=255)
                                                                                                             cagcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATaa                                 >  1:531916/1‑141 (MQ=255)
                                                                                                             cagcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTATAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATaa                                 <  2:1507731/141‑1 (MQ=255)
                                                                                                              agcagTAAAATCATTCCTAATTTGAAAACATCTTCCTGCTGATTGTTGTTGATTTCTATACATGATTAAGTTATATAATCAAACATCTTGTGTTTAATAAGTCTTACGAAATATCAACGACTTTAGTCTTTTTAGTATAAg                                >  1:486588/1‑141 (MQ=255)
                                                                                                               gcagTAAGATGATTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAgg                               >  1:697985/1‑141 (MQ=37)
                                                                                                                cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                              <  2:15737/141‑1 (MQ=255)
                                                                                                                cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                              <  1:1399026/141‑1 (MQ=255)
                                                                                                                cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                              <  1:186608/141‑1 (MQ=255)
                                                                                                                cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                              <  2:762438/141‑1 (MQ=255)
                                                                                                                 agTAAGATGATTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCg                             >  1:348363/1‑141 (MQ=38)
                                                                                                                 agTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCg                             <  1:1036357/141‑1 (MQ=255)
                                                                                                                  gTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGt                            <  2:931390/141‑1 (MQ=255)
                                                                                                                   tAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtt                           <  2:690777/141‑1 (MQ=255)
                                                                                                                     agatgaTTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt                         >  1:1250214/6‑141 (MQ=38)
                                                                                                                     agatgaTTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt                         >  1:1043900/6‑141 (MQ=38)
                                                                                                                       aTCATTCCTAATTTGGNATCATCTTACTGNTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTaa                       >  1:1328384/1‑141 (MQ=255)
                                                                                                                            tCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAAtatta                  >  2:727256/1‑141 (MQ=255)
                                                                                                                              cTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATtata                <  1:872289/141‑1 (MQ=255)
                                                                                                                                 aTTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATAtgc             >  1:1244681/1‑141 (MQ=255)
                                                                                                                                 aTTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATAtgc             >  1:610515/1‑141 (MQ=255)
                                                                                                                                   ttGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATAtgctg           <  1:732198/141‑1 (MQ=255)
                                                                                                                                    tGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATAtgcttc          >  1:1594892/1‑139 (MQ=255)
                                                                                                                                    tGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATAtgctgc          >  1:236186/1‑141 (MQ=255)
                                                                                                                                    tGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATAtgctgc          <  2:1023316/141‑1 (MQ=255)
                                                                                                                                     ggAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATgctgct         <  1:1433027/141‑1 (MQ=255)
                                                                                                                                      gAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCtt        >  2:1477848/1‑141 (MQ=255)
                                                                                                                                       aaTCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCttt       >  1:446161/1‑141 (MQ=255)
                                                                                                                                       aaTCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCttt       >  1:533332/1‑141 (MQ=255)
                                                                                                                                        atcatcTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTc      >  1:173156/1‑141 (MQ=255)
                                                                                                                                        atcatcTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTc      <  2:1373229/141‑1 (MQ=255)
                                                                                                                                         tcatcTTACTGCTGTTTGTTGTTGATTTATATTCGTGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCa     <  2:440769/141‑1 (MQ=255)
                                                                                                                                         tcatcTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCa     >  1:41376/1‑141 (MQ=255)
                                                                                                                                         tcatcTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCa     <  1:431075/141‑1 (MQ=255)
                                                                                                                                         tcatcTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCa     <  1:11580/141‑1 (MQ=255)
                                                                                                                                           atcTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCATa   <  1:1419638/141‑1 (MQ=255)
                                                                                                                                            tcTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCATaa  <  2:900229/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGCTTTCATAA  >  CP000730/942090‑942369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: