Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,669,457 G→A 6.5% A119A (GCG→GCA yicL → DMT superfamily drug/metabolite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,669,4570GA 6.5% 192.2 / 2.5 63A119A (GCG→GCAyicLDMT superfamily drug/metabolite transporter
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base A (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

AAAATGATGCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATG  >  CP000730/2669317‑2669596
                                                                                                                                            |                                                                                                                                           
aaaaTGATGCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCg                                                                                                                                             >  1:253638/1‑141 (MQ=255)
   atgatgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGAtt                                                                                                                                          <  2:531333/141‑1 (MQ=255)
      atgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                                                                       >  1:483229/1‑141 (MQ=255)
      atgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                                                                       >  2:624360/1‑141 (MQ=255)
      atgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                                                                       >  2:115931/1‑141 (MQ=255)
         cAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACa                                                                                                                                    >  2:94013/1‑141 (MQ=255)
         cAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACa                                                                                                                                    <  1:312785/141‑1 (MQ=255)
          aaCCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCAt                                                                                                                                   <  2:138075/141‑1 (MQ=255)
             ccAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtac                                                                                                                                <  1:212354/141‑1 (MQ=255)
               aaaCGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtacta                                                                                                                              <  1:115931/141‑1 (MQ=255)
                                cGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGt                                                                                                             <  2:483229/141‑1 (MQ=255)
                                  tATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGttt                                                                                                           >  1:366823/1‑141 (MQ=255)
                                      ccAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTTGTTATCTCTGCTTATATGGCATCTATTAATACAGGTATTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTatat                                                                                                       <  1:493835/141‑1 (MQ=255)
                                         aGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattat                                                                                                    >  2:225841/1‑141 (MQ=255)
                                          gATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattatt                                                                                                   <  1:386360/141‑1 (MQ=255)
                                            tCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattattat                                                                                                 <  2:80434/141‑1 (MQ=255)
                                                cGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTgg                                                                                             >  1:355484/1‑141 (MQ=255)
                                                 gTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGt                                                                                            >  2:663459/1‑141 (MQ=255)
                                                    tGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTg                                                                                         >  1:463943/1‑141 (MQ=255)
                                                                   ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                                                                          >  2:482762/1‑141 (MQ=255)
                                                                   ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                                                                          >  2:87416/1‑141 (MQ=255)
                                                                          cAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACt                                                                   >  1:676680/1‑141 (MQ=255)
                                                                           aGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                  <  1:195176/141‑1 (MQ=255)
                                                                              ataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa                                                               >  2:231827/1‑141 (MQ=255)
                                                                              ataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa                                                               >  2:290633/1‑141 (MQ=255)
                                                                                  tGGGCATGTTGGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCCCTTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat                                                           <  1:12386/141‑1 (MQ=255)
                                                                                  tGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat                                                           <  1:328547/141‑1 (MQ=255)
                                                                                       aTGTTAGTAGTACAATATGCTTTTATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAt                                                      <  1:93210/141‑1 (MQ=255)
                                                                                          ttAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATGTTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAtgtg                                                   >  2:90929/1‑141 (MQ=255)
                                                                                             gtagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgctt                                                <  1:526090/141‑1 (MQ=255)
                                                                                              tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttg                                               <  1:303465/141‑1 (MQ=255)
                                                                                              tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttg                                               >  2:593081/1‑141 (MQ=255)
                                                                                               agtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttgc                                              <  2:509852/141‑1 (MQ=255)
                                                                                                 taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCta                                            <  1:110451/141‑1 (MQ=255)
                                                                                                 taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCta                                            <  1:134585/141‑1 (MQ=255)
                                                                                                    aaTATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCtatta                                         <  1:123015/141‑1 (MQ=255)
                                                                                                        tGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCAt                                     <  1:586802/141‑1 (MQ=255)
                                                                                                         gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                    <  1:467833/141‑1 (MQ=255)
                                                                                                         gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                    <  2:223291/141‑1 (MQ=255)
                                                                                                         gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                    >  2:83846/1‑141 (MQ=255)
                                                                                                          cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                  <  1:290817/110‑1 (MQ=255)
                                                                                                          cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                  >  2:290817/1‑110 (MQ=255)
                                                                                                                  gCATCTAATGATAGAGGTAATGCTGCGATTGCAACTTTACTACAATACATTGCGCCAGTTTATATTATTATCTGGTTTGTCATAAGAGGCGTTGCAAAACTATCATTATTTGATGTGCTTGCTATTATCATGACACTATTa                           >  2:365981/1‑141 (MQ=255)
                                                                                                                    aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAgg                         <  1:469468/141‑1 (MQ=255)
                                                                                                                    aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAgg                         >  1:62175/1‑141 (MQ=255)
                                                                                                                        aTTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACa                     <  2:226667/141‑1 (MQ=255)
                                                                                                                          tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtt                   >  2:592302/1‑141 (MQ=255)
                                                                                                                          tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtt                   <  1:663459/141‑1 (MQ=255)
                                                                                                                            attgtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                 >  1:523333/7‑141 (MQ=255)
                                                                                                                            attgtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                 >  1:523337/7‑141 (MQ=255)
                                                                                                                            aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                 <  1:518247/141‑1 (MQ=255)
                                                                                                                            aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                 <  1:518221/141‑1 (MQ=255)
                                                                                                                                aGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTttatt             >  1:515827/1‑141 (MQ=255)
                                                                                                                                   tAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTttattatt          <  2:112653/141‑1 (MQ=255)
                                                                                                                                     aTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTaa        >  1:143816/1‑141 (MQ=255)
                                                                                                                                      tgctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAAc       <  2:339061/141‑1 (MQ=255)
                                                                                                                                       gctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACa      >  2:496152/1‑141 (MQ=255)
                                                                                                                                       gctgcGATTGCAACATTACTACAATACATTGCGCCAGGTTATATTATTATTTGGTTCGTCATAAGAGGCGTTGCAAAACTAACATTATCTGATGTGCTTGCTATTATCATGACACTATTAGGCACATTTTTATTATTAACa      >  2:548428/1‑141 (MQ=255)
                                                                                                                                        ctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaa     >  1:104300/1‑141 (MQ=255)
                                                                                                                                         tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaaa    >  2:607808/1‑141 (MQ=255)
                                                                                                                                         tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaaa    >  2:76279/1‑141 (MQ=255)
                                                                                                                                          gcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAAt   >  2:58554/1‑141 (MQ=255)
                                                                                                                                           cGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATg  <  2:85590/141‑1 (MQ=255)
                                                                                                                                           cGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATg  <  2:359295/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AAAATGATGCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATG  >  CP000730/2669317‑2669596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: