Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,669,460 T→C 6.4% I120I (ATT→ATC yicL → DMT superfamily drug/metabolite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,669,4600TC 6.4% 180.3 / 3.4 62I120I (ATT→ATCyicLDMT superfamily drug/metabolite transporter
Reads supporting (aligned to +/- strand):  ref base T (29/29);  new base C (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

ATGATGCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTC  >  CP000730/2669320‑2669600
                                                                                                                                            |                                                                                                                                            
atgatgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGAtt                                                                                                                                              <  2:531333/141‑1 (MQ=255)
   atgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                                                                           >  2:115931/1‑141 (MQ=255)
   atgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                                                                           >  2:624360/1‑141 (MQ=255)
   atgCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                                                                           >  1:483229/1‑141 (MQ=255)
      cAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACa                                                                                                                                        >  2:94013/1‑141 (MQ=255)
      cAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACa                                                                                                                                        <  1:312785/141‑1 (MQ=255)
       aaCCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCAt                                                                                                                                       <  2:138075/141‑1 (MQ=255)
          ccAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtac                                                                                                                                    <  1:212354/141‑1 (MQ=255)
            aaaCGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtacta                                                                                                                                  <  1:115931/141‑1 (MQ=255)
                             cGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGt                                                                                                                 <  2:483229/141‑1 (MQ=255)
                               tATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGttt                                                                                                               >  1:366823/1‑141 (MQ=255)
                                   ccAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTTGTTATCTCTGCTTATATGGCATCTATTAATACAGGTATTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTatat                                                                                                           <  1:493835/141‑1 (MQ=255)
                                      aGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattat                                                                                                        >  2:225841/1‑141 (MQ=255)
                                       gATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattatt                                                                                                       <  1:386360/141‑1 (MQ=255)
                                         tCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattattat                                                                                                     <  2:80434/141‑1 (MQ=255)
                                             cGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTgg                                                                                                 >  1:355484/1‑141 (MQ=255)
                                              gTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGt                                                                                                >  2:663459/1‑141 (MQ=255)
                                                 tGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTg                                                                                             >  1:463943/1‑141 (MQ=255)
                                                                ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                                                                              >  2:482762/1‑141 (MQ=255)
                                                                ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                                                                              >  2:87416/1‑141 (MQ=255)
                                                                       cAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACt                                                                       >  1:676680/1‑141 (MQ=255)
                                                                        aGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                      <  1:195176/141‑1 (MQ=255)
                                                                           ataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa                                                                   >  2:231827/1‑141 (MQ=255)
                                                                           ataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa                                                                   >  2:290633/1‑141 (MQ=255)
                                                                               tGGGCATGTTGGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCCCTTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat                                                               <  1:12386/141‑1 (MQ=255)
                                                                               tGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat                                                               <  1:328547/141‑1 (MQ=255)
                                                                                    aTGTTAGTAGTACAATATGCTTTTATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAt                                                          <  1:93210/141‑1 (MQ=255)
                                                                                       ttAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATGTTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAtgtg                                                       >  2:90929/1‑141 (MQ=255)
                                                                                          gtagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgctt                                                    <  1:526090/141‑1 (MQ=255)
                                                                                           tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttg                                                   >  2:593081/1‑141 (MQ=255)
                                                                                           tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttg                                                   <  1:303465/141‑1 (MQ=255)
                                                                                            agtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttgc                                                  <  2:509852/141‑1 (MQ=255)
                                                                                              taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCta                                                <  1:134585/141‑1 (MQ=255)
                                                                                              taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCta                                                <  1:110451/141‑1 (MQ=255)
                                                                                                 aaTATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCtatta                                             <  1:123015/141‑1 (MQ=255)
                                                                                                     tGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCAt                                         <  1:586802/141‑1 (MQ=255)
                                                                                                      gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                        <  2:223291/141‑1 (MQ=255)
                                                                                                      gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                        >  2:83846/1‑141 (MQ=255)
                                                                                                      gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                        <  1:467833/141‑1 (MQ=255)
                                                                                                       cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                      <  1:290817/110‑1 (MQ=255)
                                                                                                       cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                      >  2:290817/1‑110 (MQ=255)
                                                                                                               gCATCTAATGATAGAGGTAATGCTGCGATTGCAACTTTACTACAATACATTGCGCCAGTTTATATTATTATCTGGTTTGTCATAAGAGGCGTTGCAAAACTATCATTATTTGATGTGCTTGCTATTATCATGACACTATTa                               >  2:365981/1‑141 (MQ=255)
                                                                                                                 aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAgg                             <  1:469468/141‑1 (MQ=255)
                                                                                                                 aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAgg                             >  1:62175/1‑141 (MQ=255)
                                                                                                                     aTTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACa                         <  2:226667/141‑1 (MQ=255)
                                                                                                                       tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtt                       >  2:592302/1‑141 (MQ=255)
                                                                                                                       tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtt                       <  1:663459/141‑1 (MQ=255)
                                                                                                                         attgtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                     >  1:523337/7‑141 (MQ=255)
                                                                                                                         attgtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                     >  1:523333/7‑141 (MQ=255)
                                                                                                                         aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                     <  1:518221/141‑1 (MQ=255)
                                                                                                                         aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                     <  1:518247/141‑1 (MQ=255)
                                                                                                                             aGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTttatt                 >  1:515827/1‑141 (MQ=255)
                                                                                                                                tAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTttattatt              <  2:112653/141‑1 (MQ=255)
                                                                                                                                  aTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTaa            >  1:143816/1‑141 (MQ=255)
                                                                                                                                   tgctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAAc           <  2:339061/141‑1 (MQ=255)
                                                                                                                                    gctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACa          >  2:496152/1‑141 (MQ=255)
                                                                                                                                    gctgcGATTGCAACATTACTACAATACATTGCGCCAGGTTATATTATTATTTGGTTCGTCATAAGAGGCGTTGCAAAACTAACATTATCTGATGTGCTTGCTATTATCATGACACTATTAGGCACATTTTTATTATTAACa          >  2:548428/1‑141 (MQ=255)
                                                                                                                                     ctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaa         >  1:104300/1‑141 (MQ=255)
                                                                                                                                      tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaaa        >  2:607808/1‑141 (MQ=255)
                                                                                                                                      tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaaa        >  2:76279/1‑141 (MQ=255)
                                                                                                                                       gcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAAt       >  2:58554/1‑141 (MQ=255)
                                                                                                                                        cGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATg      <  2:85590/141‑1 (MQ=255)
                                                                                                                                        cGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATg      <  2:359295/141‑1 (MQ=255)
                                                                                                                                            tGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTc  <  1:369618/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATGATGCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTC  >  CP000730/2669320‑2669600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: