Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,745,325 T→A 6.3% N143I (AAT→ATT)  mqo2 ← malate dehydrogenase (acceptor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,745,3250TA 6.3% 255.6 / 5.6 79N143I (AAT→ATT) mqo2malate dehydrogenase (acceptor)
Reads supporting (aligned to +/- strand):  ref base T (37/37);  new base A (3/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ACGTGTTAATTCACCGAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCGCTTTTCACTA  >  CP000730/2745186‑2745463
                                                                                                                                           |                                                                                                                                          
aCGTGTTAATTCACCGAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGAtatt                                                                                                                                           >  1:14553/1‑141 (MQ=255)
  gtgtTAATTCACCGAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGAtattat                                                                                                                                         >  1:97292/1‑141 (MQ=255)
       aaTTCACCGAAGTTTACATCTGTACATTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATGTTCGATAATATCGaat                                                                                                                                    <  2:186314/141‑2 (MQ=255)
        aTTCACCGAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACa                                                                                                                                   >  1:474865/1‑141 (MQ=255)
            aCCGAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAgg                                                                                                                               <  2:301993/141‑1 (MQ=255)
             ccGAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAggg                                                                                                                              >  1:216958/1‑141 (MQ=255)
               gAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGTaa                                                                                                                            <  2:601602/141‑1 (MQ=255)
                 aGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAg                                                                                                                          <  1:683285/141‑1 (MQ=255)
                 aGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAg                                                                                                                          <  1:499900/141‑1 (MQ=255)
                   tttACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTCCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCt                                                                                                                        >  1:415648/1‑141 (MQ=255)
                      aCATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTc                                                                                                                     >  1:127951/1‑141 (MQ=255)
                      aCATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTc                                                                                                                     >  1:686177/1‑141 (MQ=255)
                              aCCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTTTTTTTTTTTTTTTCTTTATTATATTCGATATTATCGAACCTTGGAAAGGTTTTCATCGCttt                                                                                                             >  2:674817/1‑140 (MQ=255)
                               ccTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCg                                                                                                            <  2:474865/141‑1 (MQ=255)
                                 ttCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTa                                                                                                          >  2:253062/1‑141 (MQ=255)
                                  tCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTaa                                                                                                         >  1:349977/1‑141 (MQ=255)
                                        aTTTTCCTTGCCGACATGATACCAGGGTTCTCCTCAAGGCCTTTCATCACCCCTAGAACCCACTTTATCCTTCCTTAGATCACTTCAGTATATTCGATATTATCGAACATAGGGACAGCTTTCATCGCTTCGTAACGATCt                                                                                                   <  1:709736/141‑1 (MQ=255)
                                        aTTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCt                                                                                                   <  2:343606/141‑1 (MQ=255)
                                         ttttACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCtt                                                                                                  >  2:649162/1‑141 (MQ=255)
                                           ttACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTTGTTGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCtttt                                                                                                >  2:574559/1‑141 (MQ=255)
                                              cTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAg                                                                                             >  2:660460/1‑141 (MQ=255)
                                              cTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAg                                                                                             >  1:191558/1‑141 (MQ=255)
                                              cTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAg                                                                                             >  1:412053/1‑141 (MQ=255)
                                              cTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAg                                                                                             >  1:130126/1‑141 (MQ=255)
                                                ttccgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGaa                                                                                           <  2:628062/139‑1 (MQ=255)
                                                tGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGTAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGaa                                                                                           <  1:696179/141‑1 (MQ=255)
                                                 gccgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAt                                                                                          >  2:122033/1‑141 (MQ=255)
                                                  ccgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAtt                                                                                         >  1:1739/1‑141 (MQ=255)
                                                   cgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGCACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAttt                                                                                        <  1:577091/141‑1 (MQ=255)
                                                   cgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAttt                                                                                        >  2:118978/1‑141 (MQ=255)
                                                    gccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTa                                                                                       <  1:146394/141‑1 (MQ=255)
                                                       aTGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACa                                                                                    <  2:632026/141‑1 (MQ=255)
                                                       aTGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACa                                                                                    <  1:571728/141‑1 (MQ=255)
                                                        tGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACAt                                                                                   >  1:205274/1‑141 (MQ=255)
                                                           tACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGt                                                                                <  2:392219/141‑1 (MQ=255)
                                                            aCCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGtt                                                                               >  1:227063/1‑141 (MQ=255)
                                                             ccAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGttt                                                                              <  1:34011/141‑1 (MQ=255)
                                                             ccAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGttt                                                                              >  2:489517/1‑141 (MQ=255)
                                                             ccAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGttt                                                                              >  1:653752/1‑141 (MQ=255)
                                                             ccAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACAATGttt                                                                              >  2:575912/1‑141 (MQ=255)
                                                                     aTCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGGACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACctct                                                                      <  2:309545/141‑1 (MQ=255)
                                                                       cTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTaa                                                                    >  2:686509/1‑141 (MQ=255)
                                                                             ggCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAAc                                                              <  2:226166/141‑1 (MQ=255)
                                                                              gCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACt                                                             <  1:459327/141‑1 (MQ=255)
                                                                                 tttCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAt                                                          >  2:86850/1‑141 (MQ=255)
                                                                                 tttCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAAAATAAATGAt                                                          >  1:683169/1‑141 (MQ=255)
                                                                                     atcatcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtgtgt                                                      >  1:518346/1‑141 (MQ=255)
                                                                                       catcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTgg                                                    <  1:1823/141‑1 (MQ=255)
                                                                                       catcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTgg                                                    <  1:94068/141‑1 (MQ=255)
                                                                                       catcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTgg                                                    <  2:70907/141‑1 (MQ=255)
                                                                                         tcaATGGAATCCATTTTTTAAATACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTa                                                  <  1:180478/141‑1 (MQ=255)
                                                                                               gAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGAt                                            >  1:486416/1‑141 (MQ=255)
                                                                                                 aTCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTg                                          >  1:173425/1‑141 (MQ=255)
                                                                                                     aTTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTGGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATaa                                      <  2:170038/141‑1 (MQ=255)
                                                                                                     aTTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATaa                                      <  1:478717/141‑1 (MQ=255)
                                                                                                     aTTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATaa                                      <  1:16995/141‑1 (MQ=255)
                                                                                                     aTTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATaa                                      <  1:478569/141‑1 (MQ=255)
                                                                                                      ttttttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATaaa                                     <  1:600784/141‑1 (MQ=255)
                                                                                                      ttttttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATaaa                                     <  1:149879/141‑1 (MQ=255)
                                                                                                       tttttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAAt                                    <  1:320331/141‑1 (MQ=255)
                                                                                                        ttttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGTTTGATAAAtt                                   >  1:126184/1‑141 (MQ=255)
                                                                                                            cATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAAAATTGTTTTTACCTCTAACATAACTGATGTGTGGGAATGGATTGATAAATTctct                               >  1:49760/1‑141 (MQ=255)
                                                                                                             aTTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCCCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCtt                              >  1:293995/1‑141 (MQ=255)
                                                                                                              ttACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTg                             <  2:80828/141‑1 (MQ=255)
                                                                                                               tACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTgg                            <  1:660460/141‑1 (MQ=255)
                                                                                                               tACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATCTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTgg                            <  2:191558/141‑1 (MQ=255)
                                                                                                                 cTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAGAGATTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAATTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGt                          >  2:65545/1‑141 (MQ=255)
                                                                                                                 cTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGt                          >  1:407060/1‑141 (MQ=255)
                                                                                                                   tCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTtc                        <  1:190375/141‑1 (MQ=255)
                                                                                                                           ttCAGTATATTCGATATTATCTAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCt                <  1:618328/141‑1 (MQ=255)
                                                                                                                             cAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTAc              <  1:109422/141‑1 (MQ=255)
                                                                                                                             cAGTATATTCGATATTATCGAACATAGGCAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTATTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTAc              >  2:676986/1‑141 (MQ=255)
                                                                                                                               gTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATATTTTAAGAATTTAACATTGTTTTTACCTCTAACATAAATGATGTGTGGTAATGGATGAATAACTTCTGTTGGGTTCTCGATGCTGCCg            >  2:520748/1‑141 (MQ=255)
                                                                                                                                 atatTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCGCt          <  2:127951/141‑1 (MQ=255)
                                                                                                                                  tatTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCACtt         >  2:666204/1‑141 (MQ=255)
                                                                                                                                     tCGATAATATCGAATATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCt                >  1:241346/1‑131 (MQ=255)
                                                                                                                                     tCGATAATATCGAATATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCt                <  2:241346/131‑1 (MQ=255)
                                                                                                                                      cGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTAc                                                                          >  2:277490/1‑72 (MQ=255)
                                                                                                                                      cGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTAc                                                                          <  1:277490/72‑1 (MQ=255)
                                                                                                                                      cGATAATATCGAATATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCGCTTTTCa     >  1:214161/1‑141 (MQ=255)
                                                                                                                                       gATAATATCGAATATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCGCTTTTCAc    >  1:124535/1‑141 (MQ=255)
                                                                                                                                        atatTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCGCTTTTCACt   <  2:12083/141‑1 (MQ=255)
                                                                                                                                         tattatCGAACATAGGGAAAGCTTTCATCGCTTGGTAACACTCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGACGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCGCTTTTCACTa  >  2:378228/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ACGTGTTAATTCACCGAAGTTTACATCTGTACCTTCGTCAATTTTACTTGCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGTAATGGATTGATAAATTCTCTTGGGTTCTCGATGCTACCGCTTTTCACTA  >  CP000730/2745186‑2745463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑