Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 745,839 G→C 9.3% A46P (GCT→CCT)  yeeN → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730745,8390GC 9.3% 129.0 / 4.8 43A46P (GCT→CCT) yeeNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/17);  new base C (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

ATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTAAGATATGAAGGATTTGGCCCAAGCGGATCA  >  CP000730/745701‑745979
                                                                                                                                          |                                                                                                                                            
aTTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGACAAAAACACAAGTAGAATTTATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCt                                                                                                                                            <  2:609375/141‑1 (MQ=255)
   aTGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTa                                                                                                                                         >  2:696473/1‑141 (MQ=255)
    tGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGGATCTGACCAACCttaaa                                                                                                                                        <  1:522326/141‑4 (MQ=255)
         cGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTg                                                                                                                                   >  2:371588/1‑141 (MQ=255)
           tAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCtt                                                                                                                                           <  1:442802/131‑1 (MQ=255)
           tAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCtt                                                                                                                                           >  2:442802/1‑131 (MQ=255)
           tAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGt                                                                                                                                 >  1:58970/1‑141 (MQ=255)
                ggAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAAGTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAGTCTAACCAAGCTTTACGGTTGGTGCTTg                                                                                                                            >  1:265197/1‑141 (MQ=255)
                  aaTAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGaa                                                                                                                          <  2:108075/141‑1 (MQ=255)
                             aGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGAc                                                                                                               <  1:548811/141‑1 (MQ=255)
                                  aaaaGGCCCAAAAAGATAAAAACACAAGTAGAGTATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAACCTTAAAGCTTGGttaga                                                                                                                             <  1:149876/122‑5 (MQ=255)
                                  aaaaGGCCCAAAAAGATAAAAACACAAGTAGAGTATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAACCTTAAAGCTTGGttaga                                                                                                                             >  2:149876/1‑118 (MQ=255)
                                     aGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAg                                                                                                       <  2:631066/141‑1 (MQ=255)
                                        cccAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGc                                                                                                    >  2:362905/1‑141 (MQ=255)
                                         ccAAAAAGATAAAAACACAAGTAGAATATATGCGAAATGTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGcc                                                                                                   <  1:449331/141‑1 (MQ=255)
                                          cAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCg                                                                                                  >  1:39289/1‑141 (MQ=255)
                                                   aaaaaCACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCAtatt                                                                                         <  1:696473/141‑1 (MQ=255)
                                                   aaaaaCACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCAtatt                                                                                         <  2:566701/141‑1 (MQ=255)
                                                    aaaaCACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCAtatta                                                                                        <  1:647518/141‑1 (MQ=255)
                                                    aaaaCACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAAACTAACAACCATTCCAGGTCGGTGCTTCACAGCGCTCAGCCATATTCAGTGCCGAATCAtatta                                                                                        >  1:418124/1‑141 (MQ=255)
                                                       acacAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTg                                                                                     <  1:221186/141‑1 (MQ=255)
                                                       acacAAGTAGAATATATGCAAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTg                                                                                     <  1:389120/141‑1 (MQ=255)
                                                              tAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAACTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTAAGTGCCGAATCATATTATTGAAAAAGc                                                                              >  2:680432/1‑141 (MQ=255)
                                                                       tGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCACTAGATaa                                                                     >  1:557570/1‑141 (MQ=255)
                                                                       tGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATaa                                                                     >  1:486443/1‑141 (MQ=255)
                                                                                tGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAggg                                                            >  1:615437/1‑141 (MQ=255)
                                                                                tGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTAAAAAAGCAATAGATAAAGCTAAggg                                                            <  2:402548/141‑1 (MQ=255)
                                                                                 ggTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGt                                                           >  2:328324/1‑141 (MQ=255)
                                                                                  gTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTg                                                          >  2:283768/1‑141 (MQ=255)
                                                                                             tATGTTGCAGCAAAATCTGGCGCACCCAATCCAGGCTCTAACCACGCACTTAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTCTTGAACAAGCAATAGATAAAGCCAAGGGTTCTGGAGACGaa                                               >  2:114828/1‑141 (MQ=255)
                                                                                              aTGTTGCAGCAAAATCTGGTGAACACAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTATAGGTGCTGGAGACGaaa                                              >  1:213430/1‑141 (MQ=255)
                                                                                                     agcaAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGa                                       <  1:313436/141‑1 (MQ=255)
                                                                                                       caAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATc                                     <  2:105581/141‑1 (MQ=255)
                                                                                                         aaaTCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCAc                                   >  1:235001/1‑141 (MQ=255)
                                                                                                         aaaTCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCAc                                   >  2:648517/1‑141 (MQ=255)
                                                                                                            tcgggTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTa                                <  2:265197/138‑1 (MQ=255)
                                                                                                              tGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTAAg                              >  2:626051/1‑141 (MQ=255)
                                                                                                                      ccAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTAAGATATGAAg                      >  2:541681/1‑141 (MQ=255)
                                                                                                                           ccAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTAAGATATGAAGGAttt                 >  1:172929/1‑141 (MQ=255)
                                                                                                                               aaTCTAACCAAGCGTTAGGGTTGGTGCTGGAACGCGCTAAGACATATTCAGTGCTGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTTAGCACCTAAGATATGAAGGATTTGGcc             >  2:278370/1‑141 (MQ=255)
                                                                                                                                     aCCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCAtatt                                                                                         <  1:215753/59‑1 (MQ=255)
                                                                                                                                     aCCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCAtatt                                                                                         >  2:215753/1‑59 (MQ=255)
                                                                                                                                     aCCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTAAGATATGAAGGATTTGGCCCAAGCg       <  2:153633/141‑1 (MQ=255)
                                                                                                                                          gCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTAAGATATGAAGGATTTGGCCCAAGCGGATCa  <  1:466371/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCGAATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGATCACCTAAGATATGAAGGATTTGGCCCAAGCGGATCA  >  CP000730/745701‑745979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑