New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 1205383 =105 (0.960)85 (0.960)
+TTCCTAGGGTGCCGTCTCAGCCTCG
8/218 11.5 55.9% intergenic (+519/‑305) yfiE/lspA possible lactoylglutathione lyase/A8 family signal peptidase II
?CP000730 2478476 = 60 (0.550)intergenic (+363/+708) USA300HOU_2340/htrB hypothetical membrane protein/hemin ABC superfamily ATP binding cassette transporter, ABC protein
Rejected: Coverage evenness skew score above cutoff.

ATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGCGACA  >  CP000730/2478336‑2478612
                                                                                                                                            |                                                                                                                                        
aTGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTg                                                                                                                                          <  1:1032930/141‑1 (MQ=35)
aTGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTg                                                                                                                                          <  1:525888/141‑1 (MQ=37)
aTGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTg                                                                                                                                          >  1:1269939/1‑141 (MQ=37)
 tGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGt                                                                                                                                         >  1:1008924/1‑141 (MQ=35)
 tGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGt                                                                                                                                         <  2:698526/141‑1 (MQ=35)
 tGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGt                                                                                                                                         >  1:1305551/1‑141 (MQ=35)
   cccATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCt                                                                                                                                       <  1:154299/141‑1 (MQ=35)
      aTCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCg                                                                                                                                    >  2:1316154/1‑141 (MQ=33)
        cTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTATGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGAc                                                                                                                                  >  1:200239/1‑141 (MQ=17)
        cTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGAc                                                                                                                                  >  2:11755/1‑141 (MQ=32)
         tGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACt                                                                                                                                 >  2:683576/1‑141 (MQ=32)
           gCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTgg                                                                                                                               >  2:1332994/1‑141 (MQ=11)
                                                                      ccGTCTCAGCCACCCCTACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTACCGCGTCGCGCCATTAATACTACGTATTAACATGTAATTTTACtttt                                                                    <  1:1014265/141‑1 (MQ=11)
                                                                        gTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTaa                                                                  >  2:555867/1‑141 (MQ=32)
                                                                             aGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACt                                                             <  1:683576/141‑1 (MQ=33)
                                                                              gCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACtt                                                            <  1:893841/141‑1 (MQ=34)
                                                                              gCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACtt                                                            >  1:236398/1‑141 (MQ=34)
                                                                              gCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACtt                                                            <  2:708070/141‑1 (MQ=34)
                                                                                cACCCCGCCCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCTCTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTa                                                          <  1:1087053/141‑1 (MQ=255)
                                                                                cACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTa                                                          <  1:1035786/141‑1 (MQ=35)
                                                                                cACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTa                                                          >  1:817745/1‑141 (MQ=35)
                                                                                cACCCCAACAAGCACCTTGATGAAACCTGCCTATATGTTAGCGCCTGTGTTGGTGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTa                                                          <  1:1308014/141‑1 (MQ=11)
                                                                                  ccccAACCGGCGCAGTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCACGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTaaa                                                        >  2:420122/1‑141 (MQ=255)
                                                                                      aaCCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTaaaaaaa                                                    <  2:1036038/141‑1 (MQ=39)
                                                                                        ccGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATa                                                  >  1:322235/1‑141 (MQ=40)
                                                                                          ggCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAg                                                >  2:19508/1‑141 (MQ=255)
                                                                                            cacaTTGTTGTAGGCTGACTATATGTCAGCTTCTGTGTTGGGGGCCCTGTCTTCGACTGGCACTGCTCCCTCAGTAGTCTCGCAATTAATACTACGTATTCACATGTAATTTTACTTTTAAATACTTTAACAAAATAAGac                                              >  2:336442/1‑141 (MQ=14)
                                                                                            cacaTTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTTGGGCCCCTTTCTTCGACTGGCACTGCTCCCTCAGGCGTCTCGCCATTAATCCTACGTATTAACATGTAATTTTACTTTTAAATACTCTAAAAAAATAATac                                              >  2:34855/1‑141 (MQ=14)
                                                                                            cacaTTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGac                                              <  1:854731/141‑1 (MQ=255)
                                                                                            cacaTTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGac                                              >  1:587070/1‑141 (MQ=255)
                                                                                            cacaTTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGac                                              >  1:975460/1‑141 (MQ=255)
                                                                                            cacaTTGTTGTAAGCTGACGATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGac                                              >  2:1310952/1‑141 (MQ=255)
                                                                                               aTTGTTGTAAGCTGACTATCGGGCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACt                                           <  1:11755/141‑1 (MQ=255)
                                                                                                 tgttgtAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACttt                                         <  1:654351/141‑1 (MQ=255)
                                                                                                       aaGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACt                                                             <  1:483852/115‑1 (MQ=34)
                                                                                                       aaGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACt                                                             >  2:483852/1‑115 (MQ=34)
                                                                                                        aGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGCGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAAc                                  >  2:205303/1‑141 (MQ=255)
                                                                                                        aGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAAc                                  <  2:876478/141‑1 (MQ=255)
                                                                                                           tGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTa                               <  1:329316/141‑1 (MQ=255)
                                                                                                             acatatagTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAg                             >  2:811009/8‑141 (MQ=255)
                                                                                                             aCTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAg                             <  1:171805/141‑1 (MQ=255)
                                                                                                                atatGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCa                          <  2:1269939/141‑1 (MQ=255)
                                                                                                                  atGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAgg                        >  1:121356/1‑141 (MQ=255)
                                                                                                                  atGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAgg                        <  1:792604/141‑1 (MQ=255)
                                                                                                                    gTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACGTGTAATTTTACTTTGAAATACTTTAAAAAAATaa                                                 <  2:955196/114‑1 (MQ=255)
                                                                                                                    gTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACGTGTAATTTTACTTTGAAATACTTTAAAAAAATaa                                                 >  1:955196/1‑114 (MQ=255)
                                                                                                                      cAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATAc                    >  1:93604/1‑141 (MQ=255)
                                                                                                                      cAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATAc                    >  2:76292/1‑141 (MQ=255)
                                                                                                                      cAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATAc                    >  2:628760/1‑141 (MQ=255)
                                                                                                                       aGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACa                   <  2:1305551/141‑1 (MQ=255)
                                                                                                                       aGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACa                   <  2:1008924/141‑1 (MQ=255)
                                                                                                                          ttCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGCGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAAtt                <  1:568649/141‑1 (MQ=255)
                                                                                                                                   ggggCCCCTGTCTTCGACTGGCCCTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAg       <  2:411609/141‑1 (MQ=255)
                                                                                                                                    gggCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATTCTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGc      <  1:559342/141‑1 (MQ=255)
                                                                                                                                    gggCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGc      >  2:285548/1‑141 (MQ=255)
                                                                                                                                    gggCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGc      <  1:1234284/141‑1 (MQ=255)
                                                                                                                                     ggCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGCg     <  2:828338/141‑1 (MQ=255)
                                                                                                                                     ggCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATAATTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGCg     >  1:827152/1‑141 (MQ=255)
                                                                                                                                      gCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGCGa    >  1:914775/1‑141 (MQ=255)
                                                                                                                                        cccTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGCGACa  >  2:909155/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
ATGCCCATCTGGCACATTACTGTAAAATTCTATAAATAGAATTTTTGATGACGGGTCCCTTCCTAGGGTGCCGTCTCAGCCACCCCAACCGGCACATTGTTGTAAGCTGACTATATGTCAGCTTCTGTGTTGGGGCCCCTGTCTTCGACTGGCACTGCTCCCTCAGGAGTCTCGCCATTAATACTACGTATTAACATGTAATTTTACTTTTAAATACTTTAAAAAAATAAGACACTTTGGCCAACTTAAGCCAGGATACAATTTACAAATAGCGACA  >  CP000730/2478336‑2478612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.