| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 1,664,005 | G→A | P134L (CCT→CTT) | pbp3 ← | penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,664,005 | 0 | G | A | 95.5% | 59.6 / NA | 22 | P134L (CCT→CTT) | pbp3 | penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/11); minor base T (1/0); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTAGAAGACAATTCATCTAATTGTGATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAGGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACATTTCCGATTGTGTTGTTTTTCGACCCCTAGTATATG > CP000730/1663875‑1664137 | tttAGAAGACAATTCATCTAATTGTGATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTg < 2:403878/141‑1 (MQ=255) aagaCAATTCATCTAATTGTGATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATcc > 1:50191/1‑141 (MQ=255) aagaCAATTCATCTAATTGTGATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATcc < 2:334067/141‑1 (MQ=255) gaCAATTCATCTAATTGTGATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAg > 2:236532/1‑141 (MQ=255) aTTGTGATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTAt < 1:269723/141‑1 (MQ=255) gtgATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCAc < 1:72389/141‑1 (MQ=255) ttATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTg < 1:237390/141‑1 (MQ=255) tatTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGc < 1:295053/141‑1 (MQ=255) gATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTg > 1:20237/1‑141 (MQ=255) tCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGAt < 1:187821/141‑1 (MQ=255) tGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAAc < 1:44650/141‑1 (MQ=255) tAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACtttt > 1:285889/1‑141 (MQ=255) aTACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCa > 2:94938/1‑141 (MQ=255) aTCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCCTTATTAGTTTTGATAACTTTTCATCCGTATc > 2:262315/1‑141 (MQ=255) cTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACa > 1:314691/1‑141 (MQ=255) aCATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACAttt > 2:24064/1‑141 (MQ=255) tGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCAACTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACATTTCCGATtgt > 1:239251/1‑141 (MQ=255) tttGTCATCATTGCTTTTGCTTTTTTATTTTGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACATTTCCGATTGTgttgtt > 2:135580/1‑141 (MQ=255) tttGTCATCATTGCTTTTGCTTTTTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATTCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACATTTCCGATTGTgttgtt < 2:23160/141‑1 (MQ=255) tatTGCTTTTCCATCTTTAAGATCCATCTAAACTGAGACATCTGTATTCTCTCGTTCTGTAATTTTATATGTAAACAACTATAATAGCTTTGATAACTTTTCAGCCTTATCCAATAGTTCCGATTGGGTTGtttttgtacc < 2:191926/140‑6 (MQ=255) ttgcttttTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACATTTCCGATTGTGTTGTTTTTCGACCCCTAGtat < 1:402683/141‑1 (MQ=255) cttttTTAAGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACATTTCCGATTGTGTTGTTTTTCGACCCCTAGTATATg > 2:1678/1‑141 (MQ=255) | TTTAGAAGACAATTCATCTAATTGTGATTTTCCGATTTTCGATAACAGTTGTTTATCATATTGATCTTGTTTAATACTTCCATCTGCTAACATAGCTTGTTCTTTTGTCATCATTGCTTTTGCTTTTTTAGGATGCAACTGAATCCAGAAATCTTTCTTATCACGTTCTGTAATTTTCTTAGTATCCATCTTGATTAGCTTTGATAACTTTTCAGCCGTATCCAACATTTCCGATTGTGTTGTTTTTCGACCCCTAGTATATG > CP000730/1663875‑1664137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |