Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 36,378 C→T L58L (TTG→TTA)  tpn ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3780CT86.0% 1626.8 / 263.8 791L58L (TTG→TTA) tpnIS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (54/57);  new base T (313/367);  total (367/424)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 794 total aligned reads displayed.

GCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATC  >  CP000730/36239‑36518
                                                                                                                                             |                                                                                                                                            
gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACa                                                                                                                                             >  1:706884/1‑141 (MQ=255)
gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACa                                                                                                                                             <  2:461852/141‑1 (MQ=255)
    ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                         >  2:506921/1‑141 (MQ=255)
    ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                         >  1:833254/1‑141 (MQ=255)
    ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAACAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                         >  1:802058/1‑141 (MQ=255)
     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                          <  1:145170/141‑1 (MQ=11)
       aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                      <  2:435146/141‑1 (MQ=255)
        atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                                                                                     >  2:796077/1‑141 (MQ=255)
         tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                    <  1:879551/141‑1 (MQ=255)
         tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCACATATGATGTAAAATTgggg                                                                                                                                    >  1:283687/1‑141 (MQ=255)
          atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGc                                                                                                                                   >  2:23359/1‑141 (MQ=255)
           tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                                                                                  >  1:96357/1‑141 (MQ=255)
             tAATGTATGTCTTGCTGCATCTTTGTGTCGATATAAATGGATTCATTTTGCTTTTATTATGATGTACATCTCATCTATTAGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCATATTTGATATAAAATTGAGGCata                                                                                                                                <  2:638771/141‑1 (MQ=2)
               atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                              >  2:663623/1‑141 (MQ=255)
               atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGAAtttt                                                                                                                              >  1:651461/1‑141 (MQ=11)
               atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                              <  1:554893/141‑1 (MQ=38)
                 gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                            <  1:404090/141‑1 (MQ=255)
                  tatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt                                                                                                                           <  2:67283/141‑1 (MQ=255)
                     gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                        <  2:918203/141‑1 (MQ=21)
                     gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGaa                                                                                                                        <  2:289748/141‑1 (MQ=255)
                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                       >  1:434041/1‑141 (MQ=255)
                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                       <  2:485536/141‑1 (MQ=255)
                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                       <  1:137720/141‑1 (MQ=255)
                      tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAACATTGGGGCATATTCTTGAAc                                                                                                                       <  1:26214/141‑1 (MQ=255)
                       cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                                                                                      <  2:38196/141‑1 (MQ=255)
                       cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTATGTTTCATCTTTACGCCTTTTTTAATAAGCTTTTT‑‑TATGCTTTTTCTTTCAAATTTGATACAAAATTGGGGCATATTCTTGAAcc                                                                                                                      >  1:348170/1‑141 (MQ=14)
                       ccatctGTATCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCTATTTGTTATAATCTTTTT‑‑TATGCTTTTTCTTCCATATTTGATATTAAATTGGGGCATATTCTTGAAcc                                                                                                                      >  2:68987/4‑141 (MQ=11)
                          tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTGGAACCCaa                                                                                                                   >  1:389752/1‑141 (MQ=255)
                            tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                                                                                 >  1:845830/4‑141 (MQ=255)
                             gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                                <  1:128621/139‑1 (MQ=255)
                             gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                                <  1:694184/141‑1 (MQ=21)
                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATACGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGCAg                                                                                                             >  1:783289/1‑141 (MQ=255)
                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             <  1:86201/141‑1 (MQ=21)
                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             <  2:651463/141‑1 (MQ=25)
                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             <  1:42153/141‑1 (MQ=21)
                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             <  2:814528/141‑1 (MQ=21)
                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             >  2:331139/1‑141 (MQ=25)
                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             <  1:499283/141‑1 (MQ=19)
                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGGTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                            <  1:293820/141‑1 (MQ=255)
                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                            >  1:310043/1‑141 (MQ=21)
                                  aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGat                                                                                                           >  2:888944/1‑140 (MQ=19)
                                    tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                         <  1:682173/141‑1 (MQ=40)
                                    tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatcg                                                                                                         >  2:354001/1‑138 (MQ=255)
                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                        <  2:341157/141‑1 (MQ=255)
                                       cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                      >  2:182681/1‑141 (MQ=21)
                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGc                                                                                                                                   <  1:119288/110‑1 (MQ=255)
                                          gATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAat                                                                                                   >  2:766702/1‑141 (MQ=21)
                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATAGTTGAatg                                                                                                  >  2:869427/1‑141 (MQ=11)
                                            tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                 >  1:731227/1‑141 (MQ=255)
                                             ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                                                                                >  1:897074/1‑141 (MQ=40)
                                               aaaTAGCTCCTTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                              <  1:588911/141‑1 (MQ=255)
                                               aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                              >  1:766661/1‑141 (MQ=21)
                                                     cTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                        >  2:398664/1‑141 (MQ=255)
                                                        cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                                                                                     >  2:834968/1‑141 (MQ=21)
                                                        cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                     <  2:581479/141‑1 (MQ=255)
                                                        cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                     <  1:45842/141‑1 (MQ=255)
                                                        cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                     <  1:544475/141‑1 (MQ=255)
                                                         aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                    <  1:448552/141‑1 (MQ=21)
                                                         aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                    >  1:336107/1‑141 (MQ=255)
                                                          ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATAc                                                                                                                                              <  1:371706/82‑1 (MQ=255)
                                                          ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                   <  2:706884/141‑1 (MQ=40)
                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                   <  1:818071/141‑1 (MQ=255)
                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                   >  2:128964/1‑141 (MQ=255)
                                                           tttCCTGTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                  <  2:410668/141‑1 (MQ=255)
                                                            ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                 <  2:540509/141‑1 (MQ=18)
                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtggtgtaaac                                                                                 <  2:564879/141‑10 (MQ=11)
                                                             tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                                <  1:612692/141‑1 (MQ=21)
                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                               <  2:905020/141‑1 (MQ=21)
                                                                 tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                            >  1:526861/1‑140 (MQ=255)
                                                                 tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                            >  2:72350/1‑140 (MQ=255)
                                                                  ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                           >  1:751762/1‑141 (MQ=21)
                                                                  ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                           <  2:446343/141‑1 (MQ=21)
                                                                   tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                          <  1:671321/141‑1 (MQ=255)
                                                                    aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                         <  1:65728/141‑1 (MQ=18)
                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                         <  2:270973/141‑1 (MQ=11)
                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                         <  2:246188/141‑1 (MQ=11)
                                                                      tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                       <  2:365564/141‑1 (MQ=11)
                                                                         gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                    >  2:495261/5‑141 (MQ=11)
                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccatttcagatctacaa                                                                    >  1:632621/1‑127 (MQ=11)
                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                    >  1:829794/1‑141 (MQ=25)
                                                                          atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                   <  2:153415/138‑1 (MQ=11)
                                                                           tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                  <  1:240109/139‑1 (MQ=255)
                                                                             tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                <  2:750663/141‑1 (MQ=21)
                                                                             tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                <  2:618044/141‑1 (MQ=21)
                                                                               tttttcatcaAAACGCCATTTGTAATAAGCTTTTT‑‑TTTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGTTTTTTTTTTTATCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                              >  2:318355/5‑141 (MQ=11)
                                                                                  ctcATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                           <  1:567760/141‑1 (MQ=255)
                                                                                   tcatcaATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                          >  1:491108/1‑141 (MQ=255)
                                                                                        aaTACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCTAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                     <  1:473485/141‑1 (MQ=11)
                                                                                        aaTACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                     <  2:141358/141‑1 (MQ=21)
                                                                                         aTACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                    >  2:196986/1‑141 (MQ=255)
                                                                                             gCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                <  1:188903/141‑1 (MQ=40)
                                                                                              ccATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGGAGATCGTTGAATGATTAACGCTTACACCACGCTCTCTTAATATTTCAAATAAATCCCGATAGCt                                               >  2:851551/1‑141 (MQ=11)
                                                                                              ccATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                               >  2:161111/1‑141 (MQ=25)
                                                                                                 tttGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                            >  1:102763/1‑141 (MQ=11)
                                                                                                   tGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                          <  1:161111/141‑1 (MQ=18)
                                                                                                   tGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTGAACCCAAAGGTAGATCGTTGAATGATGAACGTATACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                          <  1:91382/141‑1 (MQ=11)
                                                                                                     taataaGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                        <  2:526861/141‑3 (MQ=11)
                                                                                                     taataaGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                        <  2:605273/141‑3 (MQ=17)
                                                                                                        taaGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTatat                                     >  1:214611/1‑141 (MQ=40)
                                                                                                                     aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTatat                                     <  1:205655/130‑1 (MQ=37)
                                                                                                                                             |                                                                                                                                            
GCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATC  >  CP000730/36239‑36518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑