Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	CP000730	2,144,161	4 bp→TGGTTTATCT	coding (480‑483/1254 nt)	sdrH ←	Ser‑Asp rich fibrinogen/bone sialoprotein bindin protein SdrH

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP000730	2144161	2144164	4	22 [0]	[0] 17	sdrH	Ser‑Asp rich fibrinogen/bone sialoprotein bindin protein SdrH

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 2144160	0 (0.000)	17 (0.540) +TGGTTTATCT	13/250	1.8	100%	coding (484/1254 nt)	sdrH	Ser‑Asp rich fibrinogen/bone sialoprotein bindin protein SdrH
	?	CP000730	2144165 =	0 (0.000)					coding (479/1254 nt)	sdrH	Ser‑Asp rich fibrinogen/bone sialoprotein bindin protein SdrH

CACCAGAATGATTGGAATCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTATTAGGGTCTGGTTTTGGATCTGGATTTGGTTTCGGGTTATCTGGATCTGGTTTTGGATCCGGAT  >  CP000730/2144028‑2144225                                                                                                                                          |                                                             aatgattggaatccccaggttgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGt                                                           >  1:219668‑M2/138‑141 (MQ=255)           atccccaggttgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTgg                                                 >  1:289374‑M2/128‑141 (MQ=255)             ccccaggttgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGAt                                               <  1:191847‑M2/16‑1 (MQ=255)               ccaggttgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggcttcggatttggttttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGGTTTGGAtcc                                             >  2:271765‑M2/124‑140 (MQ=255)                  ggttgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGATCcggt                                          >  2:161366‑M2/121‑140 (MQ=255)                    ttgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTttggatccggttt                                        <  1:33102‑M2/23‑7 (MQ=255)                    ttgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTttggatccggttt                                        <  2:202415‑M2/23‑7 (MQ=255)                     tgatcgggatctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTtttggatccggtttt                                       <  1:327699‑M2/24‑8 (MQ=255)                               ctggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGt                             <  2:92742‑M2/34‑1 (MQ=255)                                 ggtgacgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGgttt                           >  2:228004‑M2/106‑140 (MQ=255)                                     acgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTatctg                       >  1:394833‑M2/102‑141 (MQ=255)                                      cgggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGG‑TTTAGATCCGGTTTTGGTTTCGGGTTatctgga                     <  2:161156‑M2/41‑1 (MQ=255)                                       gggttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTatctgga                     >  1:86978‑M2/100‑141 (MQ=255)                                          ttaggatttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctgtgggttatcgGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTatctggatct                  >  1:236664‑M2/97‑141 (MQ=255)                                                tttggcttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGgttta            >  1:354086‑M2/91‑140 (MQ=255)                                                      ttattagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggcttgggtttatctGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGGATCTGGTTTAGgattc      >  1:337314‑M2/85‑139 (MQ=255)                                                          tagggtctggttttggattcggatttggctttggtttatctggatctggttttggatccggatttggctttggtttatctGGGTCTGGTTTTGGATCCGGTTTTGGTTTCGGGTTATCTGTATCTGGTTTAGGATTCGgac  <  1:42489‑M2/61‑2 (MQ=255)                                                                                                                                          |                                                             CACCAGAATGATTGGAATCCCCAGGTTGATCGGGATCTGGTGACGGGTTAGGATTTGGCTTATTAGGGTCTGGTTTTGGATTCGGATTTGGCTTTGGTTTATCTGGATCTGGTTTTGGATCCGGATTTGGCTTATTAGGGTCTGGTTTTGGATCTGGATTTGGTTTCGGGTTATCTGGATCTGGTTTTGGATCCGGAT  >  CP000730/2144028‑2144225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑