Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,622,711:1 +C 5.4% intergenic (‑41/+3995) USA300HOU_2485 ← / ← sarT MFS family major facilitator transporter, cation symporter/staphylococcal accessory regulator T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,622,7111.C 5.4% 413.1 / 16.0 93intergenic (‑41/+3995)USA300HOU_2485/sarTMFS family major facilitator transporter, cation symporter/staphylococcal accessory regulator T
Reads supporting (aligned to +/- strand):  ref base . (40/48);  new base C (3/2);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAACCCCCAAAATGACTACTCAGA  >  CP000730/2622572‑2622851
                                                                                                                                            |                                                                                                                                            
tattTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑c                                                                                                                                             <  2:579396/141‑1 (MQ=255)
  tttGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑Ctt                                                                                                                                           <  1:112257/141‑1 (MQ=255)
     gTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑Cttttt                                                                                                                                        <  1:476265/141‑1 (MQ=255)
     gTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑Cttttt                                                                                                                                        <  1:402624/141‑1 (MQ=255)
     gTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑Cttttt                                                                                                                                        >  1:176949/1‑141 (MQ=255)
      tAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑Ctttttt                                                                                                                                       >  1:694734/1‑141 (MQ=255)
          gAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGAc                                                                                                                                   >  2:703628/1‑141 (MQ=255)
            aaaCAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACct                                                                                                                                 <  1:790486/141‑1 (MQ=255)
             aaCAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACctt                                                                                                                                <  1:533085/141‑1 (MQ=255)
             aaCAGCACAAAACAACTCCGACGTAAGAAACTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACctt                                                                                                                                >  1:201454/1‑141 (MQ=255)
                 gCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCttcttc                                                                                                                            >  2:396784/1‑141 (MQ=255)
                 gCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCttcttc                                                                                                                            <  2:371064/141‑1 (MQ=255)
                   acaAAGCAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTtcttct                                                                                                                           <  1:84166/141‑1 (MQ=255)
                   acaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTa                                                                                                                          <  2:318480/141‑1 (MQ=255)
                   acaAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTCAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTa                                                                                                                          >  2:843895/1‑141 (MQ=255)
                       agcaacTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAAc                                                                                                                       >  1:440213/3‑141 (MQ=255)
                           acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAActctct                                                                                                                  >  2:10433/1‑141 (MQ=255)
                           acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAActctct                                                                                                                  >  2:81073/1‑141 (MQ=255)
                           acTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAActctct                                                                                                                  <  1:208387/141‑1 (MQ=255)
                                  cGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCa                                                                                                           >  1:808307/1‑141 (MQ=255)
                                     aaGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAg                                                                                                        <  2:680749/141‑1 (MQ=255)
                                       aaaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcg                                                                                                      <  1:569508/140‑1 (MQ=255)
                                        aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                                                                     <  1:683980/141‑1 (MQ=255)
                                        aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                                                                     <  2:408240/141‑1 (MQ=255)
                                        aaaTTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATAGTTATCACCTCTCATAAGAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAgcgc                                                                                                     <  2:459379/141‑1 (MQ=255)
                                           ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaa                                                                                                   >  1:124994/1‑141 (MQ=255)
                                           ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                                                                  <  1:946265/141‑1 (MQ=255)
                                           ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                                                                  <  1:297852/141‑1 (MQ=255)
                                           ttACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaat                                                                                                  <  1:324452/141‑1 (MQ=255)
                                                aatcaatAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCaattaatt                                                                                             >  1:79376/1‑141 (MQ=255)
                                                  tcaatAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCt                                                                                           <  2:592932/141‑1 (MQ=255)
                                                    aataaataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTaa                                                                                         <  2:787519/141‑1 (MQ=255)
                                                      taaataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAata                                                                                       <  1:746963/141‑1 (MQ=255)
                                                        aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAataa                                                                                      >  1:740635/1‑140 (MQ=255)
                                                        aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAAtata                                                                                     >  2:453281/1‑141 (MQ=255)
                                                        aataTGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAAtata                                                                                     <  2:591771/141‑1 (MQ=255)
                                                           atGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAAtt                                                                                  <  1:437884/141‑1 (MQ=255)
                                                              tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCTACCAC‑CTATCCTCGACATTCTTCTACACTCTCTTTATCAAAAACGCCATTAATTCTAATATAATTTaa                                                                               >  2:672031/1‑140 (MQ=255)
                                                              tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                                                                               >  2:872120/1‑141 (MQ=255)
                                                              tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                                                                               <  1:87416/141‑1 (MQ=255)
                                                              tAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAg                                                                               <  1:703628/141‑1 (MQ=255)
                                                                     tCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTa                                                                        <  1:966559/141‑1 (MQ=255)
                                                                       tATTTTTCAAAACTTCTAAGTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAGCCTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATAAAATTAAGTCAGTTaa                                                                       <  1:7775/141‑1 (MQ=255)
                                                                       tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                                                                      <  1:438497/141‑1 (MQ=255)
                                                                       tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                                                                      >  1:706897/1‑141 (MQ=255)
                                                                       tATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTaaa                                                                      >  1:471751/1‑141 (MQ=255)
                                                                       tATTTTTCAAAACTTCTAAATACAACATATTTATCACCGCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATAAACTTTAGTCAGTTaaa                                                                      >  2:236559/1‑141 (MQ=255)
                                                                        aTTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTTTTTTTTTTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAat                                                                     >  2:402296/1‑141 (MQ=255)
                                                                         tttttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGCCATTTAGGTGAACTCTCTTTATCCAAAGCGCACTTAATTCTAATATAATTTAGTCATTTAAata                                                                    >  1:836145/1‑141 (MQ=255)
                                                                         tttttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAata                                                                    >  1:176987/1‑141 (MQ=255)
                                                                           tttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATc                                                                  <  1:599164/141‑1 (MQ=255)
                                                                           tttCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATc                                                                  <  2:694734/141‑1 (MQ=255)
                                                                              cAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAat                                                               <  2:454798/141‑1 (MQ=255)
                                                                              cAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAat                                                               <  1:317751/141‑1 (MQ=255)
                                                                                 aaCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattat                                                            >  1:72357/1‑141 (MQ=255)
                                                                                 aaCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattat                                                            >  1:72368/1‑141 (MQ=255)
                                                                                  aCTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAattatt                                                           >  1:1079574/1‑141 (MQ=255)
                                                                                     tCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCg                                                        <  1:579539/141‑1 (MQ=255)
                                                                                      cTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGa                                                       <  2:68913/141‑1 (MQ=255)
                                                                                      cTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGa                                                       <  2:375442/141‑1 (MQ=255)
                                                                                          aTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAata                                                   >  1:1029786/1‑141 (MQ=255)
                                                                                          aTACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAata                                                   >  2:808325/1‑141 (MQ=255)
                                                                                           tACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAatat                                                  <  1:725896/141‑1 (MQ=255)
                                                                                             caacaTATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATatac                                                <  2:77919/141‑1 (MQ=255)
                                                                                              aacaTATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATataca                                               >  2:220707/1‑141 (MQ=255)
                                                                                                caTATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATatacatt                                             >  2:937326/1‑140 (MQ=255)
                                                                                                 atatTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATatacatac                                            <  1:730104/141‑1 (MQ=255)
                                                                                                  tatTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCATTTAAATATCAATTATTTCGAAATANACAtact                                           >  2:813961/1‑141 (MQ=255)
                                                                                                  tatTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACAtact                                           >  2:267129/1‑141 (MQ=255)
                                                                                                    tttATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACAtactac                                         <  2:279403/141‑1 (MQ=255)
                                                                                                    tccaccACCTCCCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCGTCTAAACTCTCTTTCCCCAACGCGCAATTCATTCAACTATAGTCTAGTCAGTTAACCATCAACCATTTCGAAAActacctacacc                                         >  2:277557/6‑138 (MQ=255)
                                                                                                     ttataaaaaataaTAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATactact                                        <  2:827832/129‑1 (MQ=255)
                                                                                                     ttATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATactact                                        <  2:526470/141‑1 (MQ=255)
                                                                                                     ttATCACCTATCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATAAATTATTTCGAAATATACATactact                                        <  2:526384/141‑1 (MQ=255)
                                                                                                      tATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACtt                                       <  1:101773/141‑1 (MQ=255)
                                                                                                      tATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACtt                                       <  1:160025/141‑1 (MQ=255)
                                                                                                      tATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACtt                                       <  1:326217/141‑1 (MQ=255)
                                                                                                       aTCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTg                                      <  1:528113/141‑1 (MQ=255)
                                                                                                        tCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGa                                     >  2:93629/1‑141 (MQ=255)
                                                                                                          aCCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTTGTTTTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCCGTTAAATATCAATTATTTCGAAATATACATACTACTTTaaa                                   >  2:466863/1‑141 (MQ=255)
                                                                                                          aCCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGaaa                                   >  2:582789/1‑141 (MQ=255)
                                                                                                           ccTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAac                                  <  2:264279/141‑1 (MQ=255)
                                                                                                                cATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACcat                             >  1:479865/1‑141 (MQ=255)
                                                                                                                 aTAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAGGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACcata                            >  1:987587/1‑141 (MQ=255)
                                                                                                                 aTAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACcata                            <  2:201454/141‑1 (MQ=255)
                                                                                                                     aaaTAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACcatacata                        >  1:690787/1‑141 (MQ=255)
                                                                                                                      aataatTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATaa                       <  2:338432/141‑1 (MQ=255)
                                                                                                                         aatTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAAccc                    >  2:577382/1‑141 (MQ=255)
                                                                                                                           ttGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTACACTCTCTTTATCCAAAGCGCAATTACTTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGACAACCAATACATATacccc                  >  2:373084/1‑141 (MQ=255)
                                                                                                                           ttGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAAccccc                  >  1:958357/1‑141 (MQ=255)
                                                                                                                            tGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAACCCCCa                 >  2:903712/1‑141 (MQ=255)
                                                                                                                            tGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAACCCCCa                 >  2:959756/1‑141 (MQ=255)
                                                                                                                                  cATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAACCCCCAAAATGa           <  1:795909/141‑1 (MQ=255)
                                                                                                                                      caccaG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAACCCCCAAAATGactac       <  2:887172/141‑1 (MQ=255)
                                                                                                                                           g‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAACCCCCAAAATGACTACTCaga  <  2:1077455/141‑1 (MQ=255)
                                                                                                                                           g‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTGGAAACACCATACATAACCCCCAAAATGACTACTCaga  <  2:836145/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TATTTGTAACGAAAACAGCACAAAACAACTCCGACGTAAGAAATTACCAATCAATAAATATGTAAAGTTTCTATTTTTCAAAACTTCTAAATACAACATATTTATCACCTCTCATAAAAATAATTGAATGCATCCACCAG‑CTTTTTTAGACCTTCTTCTAAACTCTCTTTATCCAAAGCGCAATTAATTCTAATATAATTTAGTCAGTTAAATATCAATTATTTCGAAATATACATACTACTTGAAACACCATACATAACCCCCAAAATGACTACTCAGA  >  CP000730/2622572‑2622851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑