Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 433,119 T→G 5.1% intergenic (‑528/+449) tcyP ← / ← USA300HOU_0408 possible DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730433,1190TG 5.1% 261.4 / 6.1 90intergenic (‑528/+449)tcyP/USA300HOU_0408possible DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/56);  new base G (3/2);  total (32/58)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

GCTATGGCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACATTTAAATT  >  CP000730/432984‑433259
                                                                                                                                       |                                                                                                                                            
gCTATGGCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCg                                                                                                                                         <  1:650502/141‑1 (MQ=255)
 cTATGGCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCgt                                                                                                                                        >  1:398873/1‑141 (MQ=255)
  tATGGCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCgtg                                                                                                                                       <  2:957922/141‑1 (MQ=255)
   aTGGCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCgtgt                                                                                                                                      >  2:236773/1‑141 (MQ=255)
     ggCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCgtgtgt                                                                                                                                    <  2:801158/141‑1 (MQ=255)
      gCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGtgtgtg                                                                                                                                   <  2:419483/141‑1 (MQ=255)
       cGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGc                                                                                                                                  >  1:606909/1‑141 (MQ=255)
         gTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAg                                                                                                                                >  2:507492/1‑141 (MQ=255)
         gTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAg                                                                                                                                <  2:590986/141‑1 (MQ=255)
              aTAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAggtggt                                                                                                                           <  1:635840/141‑1 (MQ=255)
              aTAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAggtggt                                                                                                                           <  1:937541/141‑1 (MQ=255)
              aTAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAggtggt                                                                                                                           <  2:585953/141‑1 (MQ=255)
              aTAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAggtggt                                                                                                                           >  2:772653/1‑141 (MQ=255)
              aTAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAggtggt                                                                                                                           <  1:726156/141‑1 (MQ=255)
                   tctggcgtggcTCAGAGTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTc                                                                                                                      <  2:313495/131‑1 (MQ=255)
                    aTTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCa                                                                                                                     <  1:567798/141‑1 (MQ=255)
                    aTTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGATGTTATTTCa                                                                                                                     <  2:767552/141‑1 (MQ=255)
                      tGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCaaa                                                                                                                   <  2:385119/141‑1 (MQ=255)
                       gCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGATCAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCaaaa                                                                                                                  <  2:573205/141‑1 (MQ=255)
                        cTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAg                                                                                                                 >  1:525625/1‑141 (MQ=255)
                         tAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGGGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGc                                                                                                                >  2:98553/1‑141 (MQ=255)
                               cATATTTATTTAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGt                                                                                                          <  1:490831/141‑1 (MQ=255)
                                    ttattGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACa                                                                                                     <  1:780201/141‑1 (MQ=255)
                                       ttGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAat                                                                                                  >  2:578019/1‑141 (MQ=255)
                                        tgaatgaaAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAata                                                                                                 <  1:309087/141‑1 (MQ=255)
                                          aatgaaAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACaaaaata                                                                                               <  1:429979/141‑5 (MQ=255)
                                          aatgaaAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAat                                                                                                  <  1:499908/138‑1 (MQ=255)
                                          aatgaaAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAat                                                                                                  >  2:499908/1‑138 (MQ=255)
                                           atgaaAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATAttt                                                                                              <  2:835914/141‑1 (MQ=255)
                                           aagaaAAAATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGAAGGTGGTAGTTCAAAAGCTACCGTAAACAAATAttt                                                                                              <  2:111935/139‑1 (MQ=255)
                                            tgaaAAGATAATAAATGATCAACCTTGAAAATACGTTTCGAACAAACACGTTGTAAAAAATGCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATAtttt                                                                                             >  1:432405/1‑141 (MQ=255)
                                                aaGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTtttgtt                                                                                         >  1:202963/1‑141 (MQ=255)
                                                  gATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACCTTTTTTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTtttgtttg                                                                                       <  1:556363/141‑1 (MQ=255)
                                                  gATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTtttgtttg                                                                                       <  1:743869/141‑1 (MQ=255)
                                                  gATAATAAATGATCAACCTTGAAAAGACGCTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGGGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTtttgtttg                                                                                       >  2:558080/1‑141 (MQ=255)
                                                   ataataAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTtttgtttgt                                                                                      >  2:203337/1‑140 (MQ=255)
                                                      ataaATGATCAACCTTGAAAAGACGCTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGGGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAgaa                                                                                   >  2:86924/1‑139 (MQ=255)
                                                       taaATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCaa                                                                                  >  1:151622/1‑141 (MQ=255)
                                                        aaaTGATCAACCTTGAAAAGACGCTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGGGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGaaaa                                                                                 <  1:564071/141‑1 (MQ=255)
                                                               cAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGt                                                                          >  1:341917/1‑141 (MQ=255)
                                                                aaCCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGtt                                                                         <  1:769426/141‑1 (MQ=255)
                                                                 aCCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGttt                                                                        <  1:392026/141‑1 (MQ=255)
                                                                     tGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTgtag                                                                    >  1:270624/1‑141 (MQ=255)
                                                                      gAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTgtagt                                                                   <  1:269373/141‑1 (MQ=255)
                                                                      gAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTgtagt                                                                   <  1:177043/141‑1 (MQ=255)
                                                                       aaaaGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTTAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTTCCGTAAACAAATATTTATAATTGAACAAAAAGGCGTTTgtagta                                                                  <  2:51442/141‑1 (MQ=255)
                                                                        aaaGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTAAAAAAAAAAAAAGGCGTTTGTAGTaa                                                                 <  2:332637/141‑1 (MQ=255)
                                                                             cGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAAAAAATATTCTTAATAAAACAAAAAGGCGTTTGTAGTAAAtgtg                                                            <  2:593947/141‑1 (MQ=255)
                                                                              gTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGa                                                           >  2:725403/1‑141 (MQ=255)
                                                                                ttCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAGAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAat                                                         >  1:782698/1‑141 (MQ=255)
                                                                                ttCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAat                                                         <  1:989331/141‑1 (MQ=255)
                                                                                 tCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAata                                                        <  2:355670/141‑1 (MQ=255)
                                                                                   gAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATg                                                      <  1:408685/141‑1 (MQ=255)
                                                                                     acaaacaCGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAg                                                    >  1:374995/1‑141 (MQ=255)
                                                                                     acaaacaCGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTATGTAAATGGGGTAGTGCAGAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGATTGTAGGAAATGTGAAGATGAg                                                    >  1:648488/1‑141 (MQ=255)
                                                                                        aacaCGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAgtgt                                                 <  2:544317/141‑1 (MQ=255)
                                                                                        aacaCGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAgtgt                                                 >  1:306136/1‑141 (MQ=255)
                                                                                        aacaCGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAgtgt                                                 >  1:306103/1‑141 (MQ=255)
                                                                                           acGTTGTAAAAAATTCTGTCGAATCAAAATTAGGATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTAAAAAAAAGAAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCa                                              <  2:1043773/141‑1 (MQ=255)
                                                                                            cGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCAc                                             >  1:401923/1‑141 (MQ=255)
                                                                                            cGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCAc                                             <  1:624055/141‑1 (MQ=255)
                                                                                             gTTGTAAAAAATTCTGTCGAATCAAGATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTTCCGTAAACAAATATTTTTATTAGAACAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACt                                            <  2:283049/141‑1 (MQ=255)
                                                                                              ttGTAAAAAATTCTGTCGAATCAAAATTAGCTTTTTATTTTGGTTTTTTGGTTCAGGTAGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACtt                                           <  1:209494/141‑1 (MQ=255)
                                                                                              ttGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACtt                                           <  1:438457/141‑1 (MQ=255)
                                                                                              ttGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACtt                                           <  1:559620/141‑1 (MQ=255)
                                                                                                gTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAATAAATATTTAAATAAAAAAAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTtgt                                         <  2:915577/141‑1 (MQ=255)
                                                                                                gTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTtgt                                         >  2:633790/1‑141 (MQ=255)
                                                                                                gTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGCGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAACTGTGAATATGAGTGTGCACTtgt                                         >  2:84099/1‑141 (MQ=255)
                                                                                                 tAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTtgtg                                        <  1:1055618/141‑1 (MQ=255)
                                                                                                 tAAAAAATTCTGTCGAATCAAAATTAGCATCTTGTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTtgtg                                        <  1:442875/141‑1 (MQ=255)
                                                                                                  aaaaaaTTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGc                                       <  1:772653/141‑1 (MQ=255)
                                                                                                   aaaaaTTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCg                                      <  2:975743/141‑1 (MQ=255)
                                                                                                    aaaaTTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTGCGGGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGGGAATATGAGTGTGCACTTGTGCGa                                     >  2:377171/1‑141 (MQ=255)
                                                                                                     aaaTTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGAt                                    <  1:344463/141‑1 (MQ=255)
                                                                                                        ttCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCg                                 <  1:806585/141‑1 (MQ=255)
                                                                                                        ttCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCg                                 <  2:284818/141‑1 (MQ=255)
                                                                                                        ttCTGTCGAATCAAAATTAGCATCGTTTGAGTGGTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTAAGTAAGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCg                                 <  1:24181/141‑1 (MQ=255)
                                                                                                          ttGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTAACGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTc                               <  2:480299/140‑1 (MQ=255)
                                                                                                            gTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAg                             >  2:578468/1‑141 (MQ=255)
                                                                                                             tCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGt                            >  1:23040/1‑141 (MQ=255)
                                                                                                              cGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGtt                           <  1:756156/141‑1 (MQ=255)
                                                                                                                        ttAGCATCTTTTGAGGGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTTTTTGTGAAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCCCTTGTGTGATTCGTCATTTACTAACACAg                 >  2:151435/1‑141 (MQ=255)
                                                                                                                         tAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGc                <  1:284637/141‑1 (MQ=255)
                                                                                                                          aGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGTGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGcc               >  2:318925/1‑141 (MQ=255)
                                                                                                                           gCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCa              >  1:259175/1‑141 (MQ=255)
                                                                                                                                ttttGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACAt         <  1:629992/141‑1 (MQ=255)
                                                                                                                                 tttGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACAtt        <  2:841301/141‑1 (MQ=255)
                                                                                                                                 tttGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACAtt        <  1:728894/141‑1 (MQ=255)
                                                                                                                                 tttGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACAtt        <  2:270624/141‑1 (MQ=255)
                                                                                                                                     aGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACATTTaaa    <  2:398873/141‑1 (MQ=255)
                                                                                                                                      gtgtTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACATTTAAAt   <  1:884929/141‑1 (MQ=255)
                                                                                                                                       tgtTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACATTTAAAtt  <  1:335217/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                            
GCTATGGCGGTTTGATAAAAATTGCTAACCTCATATTTATTGAATGAAAAGATAATAAATGATCAACCTTGAAAAGACGTTTCGAACAAACACGTTGTAAAAAATTCTGTCGAATCAAAATTAGCATCTTTTGAGTGTTCGTGTGTGCAGGTGGTAGTTCAAAAGCTACCGTAAACAAATATTTTTGTTTGAGCAAAAAGGCGTTTGTAGTAAATGTGAATATGAGTGTGCACTTGTGCGATTCGTCAGTTACTAACACAGCCAAACATTTAAATT  >  CP000730/432984‑433259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: