BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	434,024	T→A	5.2%	A167A (GCA→GCT)	USA300HOU_0408 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	434,024	0	T	A	5.2%	343.8 / 5.8	108	A167A (GCA→GCT)	USA300HOU_0408	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (52/50); new base A (4/2); total (56/52)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GCCTGCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATTCAT  >  CP000730/433886‑434164

gCCTGCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGc                                                                                                                                            >  2:435576/1‑141 (MQ=255)

gCCTGCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGc                                                                                                                                            >  2:472019/1‑141 (MQ=255)

gCCTGCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGc                                                                                                                                            <  1:403188/141‑1 (MQ=255)

 ccTGCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCt                                                                                                                                           <  2:77835/141‑1 (MQ=255)

  cTGCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCtt                                                                                                                                          <  1:32963/141‑1 (MQ=255)

  cTGCATTTTCTGCTTTAGTATCCGTCCTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCtt                                                                                                                                          >  2:744819/1‑141 (MQ=255)

    gCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTga                                                                                                                                        >  1:1037841/1‑141 (MQ=255)

    gCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTNTGCTTga                                                                                                                                        >  2:814460/1‑141 (MQ=255)

      aTTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTgaga                                                                                                                                      <  1:341915/141‑1 (MQ=255)

      aTTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTgaga                                                                                                                                      >  1:1058133/1‑141 (MQ=255)

      aTTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATATATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTgaga                                                                                                                                      >  1:785698/1‑141 (MQ=255)

         ttCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGc                                                                                                                                   <  2:119294/141‑1 (MQ=255)

            tGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTg                                                                                                                                >  2:132034/1‑141 (MQ=255)

            tGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTg                                                                                                                                <  1:286986/141‑1 (MQ=255)

             gCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTgc                                                                                                                               <  1:121543/141‑1 (MQ=255)

               tttAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTgcgc                                                                                                                             <  1:322850/141‑1 (MQ=255)

               tttAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTgcgc                                                                                                                             <  1:643086/141‑1 (MQ=255)

                ttAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGct                                                                                                                            <  2:874469/141‑1 (MQ=255)

                  aGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGcttc                                                                                                                          <  2:416940/141‑1 (MQ=255)

                   gTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGcttct                                                                                                                         >  1:787117/1‑141 (MQ=255)

                   gTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGcttct                                                                                                                         <  1:1012244/141‑1 (MQ=255)

                     aTCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTg                                                                                                                       >  1:890877/1‑141 (MQ=255)

                      tCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTgg                                                                                                                      >  2:518450/1‑141 (MQ=255)

                       ccGTCTTAGCGTCTGTTGATGTCTTCACTCCAGCAACCTTCGCATCTTTCCTGTCAGCTTTATCTTCAGTAACAGCATTCCTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGa                                                                                                                     <  2:1076183/141‑1 (MQ=255)

                       ccGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCtatccaagaagcgc                                                                                                                     <  1:536692/141‑14 (MQ=255)

                       ccGTCTTAGCCTCTGTTGATGTATTCACTCCAGCCATCTTCGAATCTTTTCTGTCAGCTTTATCTTCAGTAACAGTATTCTTTTCATCTTTCCTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGa                                                                                                                     <  2:1031755/141‑1 (MQ=255)

                         gTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCTGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATa                                                                                                                   >  2:674182/1‑141 (MQ=255)

                          tCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAg                                                                                                                  >  2:51013/1‑141 (MQ=255)

                           cTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGc                                                                                                                 <  1:584792/141‑1 (MQ=255)

                             tAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCct                                                                                                               >  1:453407/1‑141 (MQ=255)

                              aGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCctc                                                                                                              >  1:223180/1‑141 (MQ=255)

                                cGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCtt                                                                                                            <  1:983000/141‑1 (MQ=255)

                                 gTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTtg                                                                                                           >  1:885693/1‑141 (MQ=255)

                                 gTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTtg                                                                                                           >  1:885716/1‑141 (MQ=255)

                                    tGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGa                                                                                                        <  1:695448/141‑1 (MQ=255)

                                        taTGTATTCACTCCAGCAGCCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGAGAGCCTCTTGTGATGtt                                                                                                    <  2:86880/140‑1 (MQ=255)

                                          tGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTg                                                                                                  >  2:36596/1‑141 (MQ=255)

                                             aTTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGAt                                                                                               <  1:65687/141‑1 (MQ=255)

                                             aTTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGAt                                                                                               <  2:351160/141‑1 (MQ=255)

                                             aTTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGAt                                                                                               <  1:819569/141‑1 (MQ=255)

                                             aTTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGAt                                                                                               <  1:952394/141‑1 (MQ=255)

                                             aTTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGAt                                                                                               <  1:1029335/141‑1 (MQ=255)

                                                  cTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTCTTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCtt                                                                                          >  2:223223/1‑141 (MQ=255)

                                                    ccAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCtttt                                                                                        >  2:394798/1‑141 (MQ=255)

                                                    ccAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATCTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCtttt                                                                                        >  2:116900/1‑141 (MQ=255)

                                                    ccAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTCTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCtttt                                                                                        >  1:549981/1‑141 (MQ=255)

                                                        cAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAgg                                                                                    >  2:1061677/1‑141 (MQ=255)

                                                         aaCCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGa                                                                                   >  2:710667/1‑141 (MQ=255)

                                                          aCCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGAt                                                                                  <  1:10128/141‑1 (MQ=255)

                                                          aCCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGAt                                                                                  >  2:288821/1‑141 (MQ=255)

                                                              tCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGc                                                                              <  1:1025393/141‑1 (MQ=255)

                                                                     tttACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGAt                                                                                  >  2:517170/1‑130 (MQ=255)

                                                                     tttACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGAt                                                                                  <  1:517170/130‑1 (MQ=255)

                                                                     tttACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGa                                                                       >  2:428896/1‑141 (MQ=255)

                                                                      ttACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGaa                                                                      >  2:759080/1‑141 (MQ=255)

                                                                      ttACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGaa                                                                      <  1:463914/141‑1 (MQ=255)

                                                                       tACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAAt                                                                     >  2:419160/1‑141 (MQ=255)

                                                                       tACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAAt                                                                     <  1:735321/141‑1 (MQ=255)

                                                                        aCTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATc                                                                    <  1:522613/141‑1 (MQ=255)

                                                                             cAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGAttt                                                               >  1:344096/1‑141 (MQ=255)

                                                                             cAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGAttt                                                               <  1:288451/141‑1 (MQ=255)

                                                                             cAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGAttt                                                               >  1:668014/1‑141 (MQ=255)

                                                                                 tttATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCCAATACTTTAGCTTGAGATGCTTGTGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGa                                                           >  1:651915/1‑141 (MQ=255)

                                                                                     tcctCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAACACCTTAGCCTGAGAGGCCTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGACTCGCTTTTAGATGTa                                                       >  2:491442/4‑141 (MQ=255)

                                                                                     tCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTa                                                       >  1:430260/1‑141 (MQ=255)

                                                                                     tCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTa                                                       >  1:599737/1‑141 (MQ=255)

                                                                                       ttCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAg                                                     <  1:16871/141‑1 (MQ=255)

                                                                                       ttCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAg                                                     >  1:309233/1‑141 (MQ=255)

                                                                                       ttCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAg                                                     >  1:563048/1‑141 (MQ=255)

                                                                                         cAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGTATATCCTCTGGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGtt                                                   >  1:652394/1‑141 (MQ=255)

                                                                                         cAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGtt                                                   <  2:1045416/141‑1 (MQ=255)

                                                                                         cAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGtt                                                   <  2:26148/141‑1 (MQ=255)

                                                                                               cAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGAtttt                                             >  1:1011727/1‑141 (MQ=255)

                                                                                                aGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTg                                            <  2:401179/141‑1 (MQ=255)

                                                                                                aGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTg                                            >  2:891902/1‑141 (MQ=255)

                                                                                                   aTTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAg                                         <  1:969805/141‑1 (MQ=255)

                                                                                                    ttCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGt                                        <  2:519345/141‑1 (MQ=255)

                                                                                                        tttCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCtt                                    <  2:691038/141‑1 (MQ=255)

                                                                                                        tttCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCtt                                    <  2:734704/141‑1 (MQ=255)

                                                                                                        tttCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCtt                                    <  2:333735/141‑1 (MQ=255)

                                                                                                         ttCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCttt                                   >  1:672604/1‑141 (MQ=255)

                                                                                                         ttCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCttt                                   >  1:150095/1‑141 (MQ=255)

                                                                                                         ttCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCttt                                   >  1:418844/1‑141 (MQ=255)

                                                                                                          tCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCtttt                                  >  1:963025/1‑141 (MQ=255)

                                                                                                             aCTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAAt                               >  1:5876/1‑141 (MQ=255)

                                                                                                               tttATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTCTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTc                             <  2:572279/141‑1 (MQ=255)

                                                                                                                   tttGTTGATTCAGCTAATGCTTTTGCTTGTGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCttgt                         <  1:726271/141‑1 (MQ=255)

                                                                                                                     tgttgATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCttgttg                       >  2:116122/1‑141 (MQ=255)

                                                                                                                      gttgATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCttgttgt                      >  1:882824/1‑141 (MQ=255)

                                                                                                                             cAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGAt               <  2:556097/141‑1 (MQ=255)

                                                                                                                              aGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATg              >  2:178381/1‑141 (MQ=255)

                                                                                                                               gccaatacTTTAGCTTGAGATGCTTGTGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGt             >  2:657346/8‑141 (MQ=255)

                                                                                                                               gCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGt             >  1:742966/1‑141 (MQ=255)

                                                                                                                                     gCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGa       >  1:183671/1‑141 (MQ=255)

                                                                                                                                        tttGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATtc    <  1:46398/141‑1 (MQ=255)

                                                                                                                                         ttGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATtca   <  1:727214/141‑1 (MQ=255)

                                                                                                                                          tGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATtcat  <  2:310112/141‑1 (MQ=255)

                                                                                                                                          tGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATtcat  >  1:548868/1‑141 (MQ=255)

                                                                                                                                          tGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATtcat  >  2:891653/1‑141 (MQ=255)

                                                                                                                                          tGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATtcat  <  2:625786/141‑1 (MQ=255)

GCCTGCATTTTCTGCTTTAGTATCCGTCTTAGCGTCTGTTGATGTATTCACTCCAGCAACCTTCGAATCTTTACTGTCAGCTTTATCTTCAGTAACAGCATTCTTTTCAACTTTATTTGTTGATTCAGCTAATGCTTTTGCTTGAGAGGCTTGCGCTTCTTGGATAGCCTCTTGTGATGTTTGGATGGCTTTTAAGGATTCGCTATTTGAATCGATTTTAGATGTAAGTTGATTTTGAAGTTCTTTTAATTCTTGTTGTTGCTGATGTACTTGATTCAT  >  CP000730/433886‑434164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`